FB2024_03 , released June 25, 2024
Allele: Dmel\Mmp1E225A.f1.UAS
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General Information
Symbol
Dmel\Mmp1E225A.f1.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0193507
Feature type
allele
Associated gene
Dmel\Mmp1
Associated Insertion(s)
Carried in Construct
P{UAS-Mmp1.E225A.f1}
Key Links
Transgenic product class
missense_variant
(Zhang et al., 2006)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Zhang et al., 2006)
Mutagen
in vitro construct
(Zhang et al., 2006)
Progenitor genotype
Carried in construct
P{UAS-Mmp1.E225A.f1}
Cytology
Description

Amino acid replacement: E225A.

(Zhang et al., 2006)

UAS regulatory sequences drive expression of mutated, full-length Mmp1 coding sequences, corresponding to isoform 1 of the protein.

(Zhang et al., 2006)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Mmp1
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Almost no third instar larvae expressing Mmp1E225A.f1.Scer\UAS under the control of Scer\GAL4αTub84B.PL display broken tracheal dorsal trunks. 88% survive to pupariation. The majority of dissected pupae show defects in head eversion.

      Expression of Mmp1E225A.f1.Scer\UAS under the control of Scer\GAL4αTub84B.PL strongly enhances the phenotype of Mmp1Q273stop/Mmp1W439stop transheterozygous mutant third instar larvae. 15% display broken tracheal dorsal trunks and appear sluggish, and the majority of dissected pupae show defects in wing and head eversion. These phenotypes are stronger than either Mmp1Q273stop/Mmp1W439stop or Scer\GAL4αTub84B.PL driving Mmp1E225A.f1.Scer\UAS alone.

      Very few third instar larvae expressing Mmp1E225A.f1.Scer\UAS under the control of Scer\GAL4btl.PS display broken tracheal dorsal trunks.

      (Glasheen et al., 2009)

      Scer\GAL4elav.PU- or Scer\GAL4repo.PU-mediated expression of Mmp1E225A.f1.Scer\UAS results in aberrant ISNb morphology - defasciculation with loose bundling and ectopic branches.

      (Miller et al., 2008)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of Mmp1E225A.f1.MtnA, with NijAA.MtnA.T:Ivir\HA1 suppresses the loss of cellular adherence observed in NijAA.MtnA.T:Ivir\HA1 mutants.

      (Zhang et al., 2006)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      "UAS-Mmp1.f1E225A" is transfected into S2 cells to study cell adhesion pathways.

      (Zhang et al., 2006)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Mmp1E225A.f1.Scer\UAS
      Mmp1E225A.f1.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (4)