FlyBase Allele Report: Dmel\Mmp2[Y53N]

General Information

Symbol

Dmel\Mmp2^Y53N

Species

D. melanogaster

Name

FlyBase ID

FBal0299609

Feature type

allele

Associated gene

Dmel\Mmp2

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Wang and Page-McCaw, 2014)

Progenitor genotype

Cytology

Description

Amino acid replacement: Y53N.

(Wang and Page-McCaw, 2014)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:9,667,768..9,667,768 [-]

Nucleotide change:

T9667768A

Amino acid change:

Y53N | Mmp2-PB

Reported amino acid change:

Y53N

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. Mutation mapped relative to isoform Mmp2-PB.

(Wang and Page-McCaw, 2014)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell number | adult stage | female | heat sensitive (with Df(2R)BSC132)

(Wang and Page-McCaw, 2014)

increased cell number | adult stage | female | heat sensitive (with Mmp2²¹⁸)

(Wang and Page-McCaw, 2014)

increased cell number | adult stage | female | heat sensitive (with Mmp2⁰²³⁵³)

(Wang and Page-McCaw, 2014)

increased cell number | adult stage | female | heat sensitive (with Mmp2^W307stop)

(Wang and Page-McCaw, 2014)

increased cell number | oogenesis | heat sensitive (with Df(2R)BSC132)

(Wang and Page-McCaw, 2014)

increased cell number | oogenesis | heat sensitive (with Mmp2²¹⁸)

(Wang and Page-McCaw, 2014)

increased cell number | oogenesis | heat sensitive (with Mmp2^W307stop)

(Wang and Page-McCaw, 2014)

increased occurrence of cell division | adult stage | female | heat sensitive (with Mmp2^W307stop)

(Wang and Page-McCaw, 2014)

short lived (with Df(2R)BSC132)

(Wang and Page-McCaw, 2014)

short lived (with Mmp2²¹⁸)

(Wang and Page-McCaw, 2014)

Phenotype Manifest In

Detailed Description

Statement

Reference

Mmp2^Y53N/Mmp2^W307stop, Mmp2^Y53N/Mmp2²¹⁸, Mmp2^Y53N/Df(2R)BSC132 and Mmp2^Y53N/Mmp2⁰²³⁵³ females show increased numbers of stalk cells between egg chambers compared to wild type when assayed 7-9 days after being shifted to the restrictive temperature.

Mmp2^Y53N/Mmp2^W307stop females shifted to the restrictive temperature show increased follicle precursor cell proliferation in region 2b of the germarium compared to controls. The mutant follicle stem cells show a significant increase in cell division compared to controls, while the cell division rate of mutant germline stem cells is normal.

(Wang and Page-McCaw, 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Mmp2^Y53N/Mmp2^W307stop has increased cell number | adult stage | female | heat sensitive phenotype, enhanceable by dlp^UAS.cBa/Scer\GAL4^C587

(Wang and Page-McCaw, 2014)

Suppressed by

Statement

Reference

Df(2R)BSC132/Mmp2^Y53N has increased cell number | female | adult stage | heat sensitive phenotype, suppressible by dlp[+]/dlp²

(Wang and Page-McCaw, 2014)

Df(2R)BSC132/Mmp2^Y53N has increased cell number | female | adult stage | heat sensitive phenotype, suppressible by dlp¹/dlp[+]

(Wang and Page-McCaw, 2014)

Df(2R)BSC132/Mmp2^Y53N has increased cell number | female | adult stage | heat sensitive phenotype, suppressible by wg[+]/wg^l-8

(Wang and Page-McCaw, 2014)

Df(2R)BSC132/Mmp2^Y53N has increased cell number | female | adult stage | heat sensitive phenotype, suppressible by wg[+]/wg^l-17

(Wang and Page-McCaw, 2014)

Mmp2^Y53N/Mmp2^W307stop has increased cell number | female | adult stage | heat sensitive phenotype, suppressible by arm²/arm[+]

(Wang and Page-McCaw, 2014)

Mmp2^Y53N/Mmp2^W307stop has increased cell number | female | adult stage | heat sensitive phenotype, suppressible by dlp[+]/dlp²

(Wang and Page-McCaw, 2014)

Mmp2^Y53N/Mmp2^W307stop has increased cell number | female | adult stage | heat sensitive phenotype, suppressible by dlp¹/dlp[+]

(Wang and Page-McCaw, 2014)

Phenotype Manifest In

Enhanced by

Statement

Reference

Mmp2^Y53N/Mmp2^W307stop has stalk follicle cell | increased number | heat sensitive phenotype, enhanceable by dlp^UAS.cBa/Scer\GAL4^C587

(Wang and Page-McCaw, 2014)

Suppressed by

Statement

Reference

Df(2R)BSC132/Mmp2^Y53N has stalk follicle cell | increased number | heat sensitive phenotype, suppressible by wg[+]/wg^l-8

(Wang and Page-McCaw, 2014)

Df(2R)BSC132/Mmp2^Y53N has stalk follicle cell | increased number | heat sensitive phenotype, suppressible by wg[+]/wg^l-17

(Wang and Page-McCaw, 2014)

Mmp2^Y53N/Mmp2^W307stop has stalk follicle cell | increased number | heat sensitive phenotype, suppressible by arm²/arm[+]

(Wang and Page-McCaw, 2014)

Df(2R)BSC132/Mmp2^Y53N has stalk follicle cell | increased number | heat sensitive phenotype, suppressible by dlp¹/dlp[+]

(Wang and Page-McCaw, 2014)

Df(2R)BSC132/Mmp2^Y53N has stalk follicle cell | increased number | heat sensitive phenotype, suppressible by dlp[+]/dlp²

(Wang and Page-McCaw, 2014)

Mmp2^Y53N/Mmp2^W307stop has stalk follicle cell | increased number | heat sensitive phenotype, suppressible by dlp¹/dlp[+]

(Wang and Page-McCaw, 2014)

Mmp2^Y53N/Mmp2^W307stop has stalk follicle cell | increased number | heat sensitive phenotype, suppressible by dlp[+]/dlp²

(Wang and Page-McCaw, 2014)

Additional Comments

Genetic Interactions

Statement

Reference

The increase in average cell number in the stalks between egg chambers which is seen in Mmp2^Y53N/Df(2R)BSC132 females after being shifted to the restrictive temperature is dominantly suppressed if the females also carry a single copy of either wg^l-8, wg^l-17, dlp¹ or dlp².

The increase in average cell number in the stalks between egg chambers which is seen in Mmp2^Y53N/Mmp2^W307stop females after being shifted to the restrictive temperature is dominantly suppressed if the females also carry a single copy of either arm², dlp¹ or dlp².

The increase in average cell number in the stalks between egg chambers which is seen in Mmp2^Y53N/Mmp2^W307stop females after being shifted to the restrictive temperature is enhanced if they are also expressing dlp^Scer\UAS.cBa under the control of Scer\GAL4^C587.

(Wang and Page-McCaw, 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Df(2R)BSC132/Mmp2^Y53N is rescued by Mmp2^GPI.EGFP

(Wang and Page-McCaw, 2014)

Comments

Mmp2^{GPI.T:Avic\GFP-EGFP} completely rescues the pupal lethality of Mmp2 mutants. It rescues the lifespan, fertility and follicle cell defects of Mmp2^Y53N/Df(2R)BSC132 flies.

(Wang and Page-McCaw, 2014)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Mmp2^Y53N

(Wang and Page-McCaw, 2014)

Name Synonyms

Secondary FlyBase IDs

References (1)

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