FlyBase Allele Report: Dmel\dlp[1]

General Information

Symbol

Dmel\dlp¹

Species

D. melanogaster

Name

FlyBase ID

FBal0190800

Feature type

allele

Associated gene

Dmel\dlp

Associated Insertion(s)

P{?PZ}dlp¹

Carried in Construct

Key Links

Allele class

loss of function allele

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

loss of function allele

(Kirkpatrick et al., 2004)

Mutagen

Delta2-3 transposase

(Kirkpatrick et al., 2004)

Progenitor genotype

P{PZ}Q5

(Kirkpatrick et al., 2004)

Associated Insertion(s)

P{?PZ}dlp¹

Cytology

Description

Imprecise excision of the P{PZ}Q5 element, resulting in a 504bp deletion that begins at the insertion site and extends 2bp beyond the putative ATG for the dlp open reading frame. 8bp of the P{PZ} element inverted repeat sequence remain.

(Kirkpatrick et al., 2004)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

denticle | ectopic | ventral (with dlp²)

(Kirkpatrick et al., 2004)

embryonic/first instar larval cuticle (with dlp²)

(Kirkpatrick et al., 2004)

eye (with Df(3L)fz-D21)

(Rawson, 2005)

eye photoreceptor cell & axon (with Df(3L)fz-D21)

(Rawson et al., 2005)

eye photoreceptor cell & axon (with dlp²)

(Rawson, 2005)

interommatidial bristle (with Df(3L)fz-D21)

(Rawson, 2005)

lamina plexus (with Df(3L)fz-D21)

(Rawson et al., 2005)

lamina plexus (with dlp²)

(Rawson, 2005)

medulla (with Df(3L)fz-D21)

(Rawson et al., 2005)

medulla (with dlp²)

(Rawson, 2005)

ommatidium (with Df(3L)fz-D21)

(Rawson, 2005, Kirkpatrick et al., 2004)

presynaptic active zone | third instar larval stage (with dlp²)

(Johnson et al., 2006)

wing disc (with Df(3L)fz-D21)

(Kirkpatrick et al., 2004)

wing margin bristle, with Scer\GAL4^ap-md544, dlp^UAS.cBa

(Kirkpatrick et al., 2004)

wing margin bristle | ectopic (with Df(3L)fz-D21)

(Kirkpatrick et al., 2004)

wing margin bristle | ectopic (with dlp²)

(Kirkpatrick et al., 2004)

Detailed Description

Statement

Reference

dlp¹/dlp² larvae have a normal number of boutons at the neuromuscular junction. Mean active zone area is significantly decreased and the number of active zones per bouton is significantly increased compared to wild type.

The amplitude of the evoked excitatory junctional potential (EJP) is increased compared to wild type at the neuromuscular junction of dlp¹/dlp² larvae. The amplitude and frequency of miniature EJPs is normal. Quantal content is increased.

(Johnson et al., 2006)

dlp¹/Df(3L)fz-D21 mutants show axon guidance defects. At the third instar larval stage, half of mutants show thickening of the lamina plexus, and 80% show crossovers between ommatidial axon bundles and abnormal photoreceptor process expansion outside the lamina plexus. Mosaic studies show that dlp¹ homozygous photoreceptors misguide axons to the lamina plexus, but do not affect axon guidance to the medulla. At the pupal level, 80% of dlp¹/Df(3L)fz-D21 mutants have brains with irregularities in the R7 and R8 medulla termini, ~50% show medulla cartridge crossover and ~20% show misrouting of R7/R8 axons. Electrophysiological analysis shows that dlp¹/Df(3L)fz-D21 flies have grossly abnormal ERGs, with defective photoreceptor polarization and complete absence of on- and off-transients. Mosaic flies with dlp¹ mutant photoreceptors and wild-type brains have normal ERGs, suggesting that the ERG defect is due to the presence of mutation in the optic lobe, not the eye.

(Rawson et al., 2005)

dlp¹/dlp² mutants show axon guidance defects. At the third instar larval stage, ~30% of mutants show thickening of the lamina plexus, and >60% show crossovers between ommatidial axon bundles and abnormal photoreceptor process expansion outside the lamina plexus. At the pupal level, 80% of dlp¹/dlp² mutants have brains with irregularities in the R7 and R8 medulla termini and over 20% show medulla cartridge crossover. The eyes of dlp¹/Df(3L)fz-D21 adults show a rough eye phenotype with eyes that are reduced in size. However, the mutant eyes contain all photoreceptors and ommatidial polarity is normal. Differentiation of dlp¹/dlp² photoreceptors is not defective.

(Rawson, 2005)

Less than 5% of dlp¹ mutants survive to adulthood. dlp¹/dlp² embryos from dlp¹ germline clone females and dlp² males show a severe segment polarity defect with denticles covering the ventral surface. Rare dlp¹/Df(3L)fz-D21 escapers show a ~50% reduction in ommatidia and disordered ommatidial structure compared to wild type. Both dlp¹/dlp² and dlp¹/Df(3L)fz-D21 escapers show patterning defects at the anterior margin, with ectopic mechanosensory bristles on both dorsal and ventral surfaces, that are positioned further from the wing margin than bristles in wild type. This phenotype is fully penetrant for both mutants, but the number of ectopic bristles varies. Expression of dlp^Scer\UAS.cBa, under the control of Scer\GAL4^ap-md544, in a dlp¹/+ background leads to bristle loss at the anterior wing margin. BrdU incorporation shows that the zone of nonproliferating cells along the DV boundary of the third instar wing disc in dlp¹/Df(3L)fz-D21 larvae is expanded compared to dlp¹/+ controls. The average width of the wing pouch is reduced in these mutants.

(Kirkpatrick et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

dlp¹/dlp[+] is a non-enhancer of abnormal neuroanatomy | embryonic stage phenotype of Scer\GAL4^P52, Sema1a^UAS.cYa, Sema1a^k13702

(Cho et al., 2012)

Suppressor of

Statement

Reference

dlp¹/dlp[+] is a suppressor of increased cell number | female | adult stage | heat sensitive phenotype of Mmp2^Y53N/Mmp2^W307stop

(Wang and Page-McCaw, 2014)

dlp¹/dlp[+] is a suppressor of increased cell number | female | adult stage | heat sensitive phenotype of Df(2R)BSC132/Mmp2^Y53N

(Wang and Page-McCaw, 2014)

NOT Suppressor of

Statement

Reference

dlp¹/dlp[+] is a non-suppressor of abnormal neuroanatomy | embryonic stage phenotype of Scer\GAL4^P52, Sema1a^UAS.cYa, Sema1a^k13702

(Cho et al., 2012)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

dlp¹/dlp² has eye photoreceptor cell & axon phenotype, non-suppressible by Sdc^UAS.cSa/Scer\GAL4^da.G32

(Rawson et al., 2005)

NOT Enhancer of

Statement

Reference

dlp¹/dlp[+] is a non-enhancer of larval posterior commissure | embryonic stage phenotype of Scer\GAL4^P52, Sema1a^UAS.cYa, Sema1a^k13702

(Cho et al., 2012)

dlp¹ is a non-enhancer of anterior fascicle & axon | ectopic phenotype of Lar^bypass

(Fox and Zinn, 2005)

Suppressor of

Statement

Reference

dlp¹/dlp[+] is a suppressor of stalk follicle cell | increased number | heat sensitive phenotype of Df(2R)BSC132/Mmp2^Y53N

(Wang and Page-McCaw, 2014)

dlp¹/dlp[+] is a suppressor of stalk follicle cell | increased number | heat sensitive phenotype of Mmp2^Y53N/Mmp2^W307stop

(Wang and Page-McCaw, 2014)

dlp¹/dlp[+] is a suppressor of wing sensillum phenotype of Hsepi^d12

(Dejima et al., 2013)

dlp¹/dlp[+] is a suppressor | partially of wing phenotype of Notum^UAS.cGa, Scer\GAL4^ap-md544

(Kirkpatrick et al., 2004)

NOT Suppressor of

Statement

Reference

dlp¹/dlp[+] is a non-suppressor of larval posterior commissure | embryonic stage phenotype of Scer\GAL4^P52, Sema1a^UAS.cYa, Sema1a^k13702

(Cho et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

The increase in average cell number in the stalks between egg chambers which is seen in Mmp2^Y53N/Df(2R)BSC132 and Mmp2^Y53N/Mmp2^W307stop females after being shifted to the restrictive temperature is dominantly suppressed if the females also carry a single copy of dlp¹.

(Wang and Page-McCaw, 2014)

A heterozygous dlp¹

background does not result in a decreased number of chemosensory bristles in a Hsepi^d12 homozygous mutants.

(Dejima et al., 2013)

The commissural axon phenotype (failure to cross the midline) seen in embryos expressing Sema-1a^Scer\UAS.cYa under the control of Scer\GAL4^P52 in a Sema-1a^k13702 null background is not affected if the embryos are also heterozygous for dlp¹.

(Cho et al., 2012)

The dlp¹ mutation does not increase the frequency of the Lar^bypass ISNb bypass phenotype.

(Fox and Zinn, 2005)

Expression of Sdc^Scer\UAS.cSa, under the control of Scer\GAL4^da.G32, fails to rescue the axon guidance defects of dlp¹/dlp² mutants.

(Rawson et al., 2005)

Heterozygosity for dlp¹ partially suppresses the wingless phenotype seen when Notum^Scer\UAS.cGa is expressed under the control of Scer\GAL4^ap-md544. Approximately 40% of such flies display a winglet on one side of the body, but are usually wingless on the other side. These winglets often display substantially normal wing patterning, although this phenotype is variable.

(Kirkpatrick et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

dlp²

(Kirkpatrick et al., 2004)

Rescued by

dlp¹ is rescued by dlp^UAS.Tag:HA/Scer\GAL4^ey.PH

(Rawson et al., 2005)

dlp¹ is rescued by dlp^UAS.Tag:HA

(Rawson et al., 2005)

dlp¹/dlp² is rescued by dlp^UAS.cBa/Scer\GAL4^ap-md544

(Kirkpatrick et al., 2004)

dlp¹/Df(3L)fz-D21 is rescued by dlp^UAS.cBa/Scer\GAL4^ap-md544

(Kirkpatrick et al., 2004)

Partially rescued by

dlp¹/Df(3L)fz-D21 is partially rescued by dlp^UAS.cBa

(Kirkpatrick et al., 2004)

Comments

Expression of dlp^Scer\UAS.cBa without a driver is sufficient to rescue the eye phenotype, but not the wing phenotype of dlp¹/Df(3L)fz-D21 mutants.

(Kirkpatrick et al., 2004)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

dlp¹

(Kanai et al., 2018, Wang and Page-McCaw, 2014, Dejima et al., 2013, Cho et al., 2012, Wojcinski et al., 2011, Ren et al., 2009, Johnson et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (11)

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