FlyBase Allele Report: Dmel\Lar[bypass]

General Information

Symbol

Dmel\Lar^bypass

Species

D. melanogaster

Name

FlyBase ID

FBal0086815

Feature type

allele

Associated gene

Dmel\Lar

Associated Insertion(s)

Carried in Construct

Also Known As

Dlar^bypass, Dlar^byp

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Krueger et al., 2003)

Mutagen

Progenitor genotype

Cytology

Description

4bp deletion between the two PTPase domains of Lar, leading to truncation of the gene product shortly before the beginning of PTP-D2.

(Krueger et al., 2003)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2L:19,729,085..19,729,088 [+]

Comment:

Position of mutation on reference sequence inferred by FlyBase curator based on author statement (Figure 4). 4bp deletion between the two PTPase domains of Lar, leading to truncation of the gene product shortly before the beginning of PTP-D2.

(Krueger et al., 2003)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy

(Kaufmann et al., 2002)

abnormal neuroanatomy (with Lar²¹²⁷)

(Hofmeyer et al., 2006)

abnormal neuroanatomy | cell autonomous | pupal stage | somatic clone

(Prakash et al., 2009)

partially lethal - majority die | recessive

(Krueger et al., 2003)

Phenotype Manifest In

anterior fascicle & axon | ectopic

(Fox and Zinn, 2005, Fox and Zinn, 2005)

bouton

(Kaufmann et al., 2002)

larval intersegmental nerve

(Wills et al., 1999)

neuromuscular junction

(Kaufmann et al., 2002)

photoreceptor cell R7 (with Lar²¹²⁷)

(Hofmeyer et al., 2006)

photoreceptor neuron | cell autonomous | pupal stage | somatic clone

(Prakash et al., 2009)

Detailed Description

Statement

Reference

43% of pupal R1-R6 axons mutant for Lar^bypass do not extend their axons, or show aberrant axon extension, targeting, and morphology.

(Prakash et al., 2009)

Stage 14 oocytes from Lar²¹²⁷/Lar^bypass females are indistinguishable from wild-type.

(Hofmeyer, 2006)

Around 10% of Lar^bypass mutants show an ISNb bypass phenotype.

(Fox and Zinn, 2005)

Around 10% Lar^bypass mutants display a motor axon guidance phenotype known as "ISNb bypass" in which ISNb axons successfully split away from the ISN pathway at the exit junction, but can fail to grow into the muscle field at their normal entry point. Instead, such axons travel along parallel to the ISN, underneath the muscles, until they reach the dorsal edge of the VLM field.

(Fox and Zinn, 2005)

Homozygous and heterozygous larvae have fewer boutons (both type Ib and type Is) at the muscle 6/7 neuromuscular junction than wild type.

(Kaufmann et al., 2002)

ISNb axons often show a "bypass" phenotype (failing to enter the normal muscle target domain just outside the ventral nerve cord and instead following the intersegmental nerve towards dorsal targets) in homozygous embryos. ISNb axons also show a bypass phenotype in Lar^bypass/Lar^5.5 or Lar^bypass/Lar^13.2 embryos. ISNb axons also show a bypass phenotype in Lar^bypass/Lar^E55 embryos.

(Wills et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Lar^bypass/Lar^bypass is a suppressor of abnormal neuroanatomy phenotype of Abl²

(Wills et al., 2002)

Phenotype Manifest In

Enhanced by

Statement

Reference

Lar^bypass has anterior fascicle & axon | ectopic phenotype, enhanceable by Sdc⁴⁸

(Fox and Zinn, 2005)

Lar^bypass has anterior fascicle & axon | ectopic phenotype, enhanceable by Sdc^KG06163

(Fox and Zinn, 2005)

Lar^bypass has anterior fascicle & axon | ectopic phenotype, enhanceable by Sdc¹⁰⁶⁰⁸

(Fox and Zinn, 2005)

Lar^bypass has phenotype, enhanceable by Scer\GAL4^elav-C155/Rac1^N17.UAS

(Kaufmann et al., 1998)

NOT Enhanced by

Statement

Reference

Lar^bypass has anterior fascicle & axon | ectopic phenotype, non-enhanceable by Fkbp14⁰⁰⁷³⁴

(Fox and Zinn, 2005)

Lar^bypass has anterior fascicle & axon | ectopic phenotype, non-enhanceable by dally⁰⁶⁴⁶⁴

(Fox and Zinn, 2005)

Lar^bypass has anterior fascicle & axon | ectopic phenotype, non-enhanceable by dlp¹

(Fox and Zinn, 2005)

Lar^bypass, dlp^A187 has anterior fascicle & axon | ectopic phenotype, non-enhanceable by dally⁸⁰

(Fox and Zinn, 2005)

Lar^bypass, dally⁸⁰ has anterior fascicle & axon | ectopic phenotype, non-enhanceable by dlp^A187

(Fox and Zinn, 2005)

Lar^bypass has anterior fascicle & axon | ectopic phenotype, non-enhanceable by Sara²⁵⁰

(Fox and Zinn, 2005)

Suppressed by

Statement

Reference

Lar^bypass/Lar^5.5 has larval intersegmental nerve phenotype, suppressible by Abl¹

(Wills et al., 1999)

Lar^bypass/Lar^13.2 has larval intersegmental nerve phenotype, suppressible by Abl¹

(Wills et al., 1999)

Lar^bypass/Lar^E55 has larval intersegmental nerve phenotype, suppressible by Abl¹

(Wills et al., 1999)

Lar^bypass/Lar^E55 has larval intersegmental nerve phenotype, suppressible by Abl²

(Wills et al., 1999)

Lar^bypass/Lar^E55 has larval intersegmental nerve phenotype, suppressible by Abl⁴

(Wills et al., 1999)

Lar^bypass has larval intersegmental nerve phenotype, suppressible by Abl¹

(Wills et al., 1999)

Suppressor of

Statement

Reference

Lar^bypass/Lar^bypass is a suppressor of presumptive embryonic/larval central nervous system phenotype of Abl²

(Wills et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

The following mutations do not increase the frequency of the Lar^bypass ISNb bypass phenotype: Sara²⁵⁰, Fkbp13⁰⁰⁷³⁴, dally⁰⁶⁴⁶⁴, and dlp¹. The Lar^bypass ISNb phenotype is also not enhanced in Lar^bypass; dlp^A187, dally⁸⁰ triple mutants.

(Fox and Zinn, 2005)

Sdc alleles (Sdc¹⁰⁶⁰⁸, Sdc^KG06163 and Sdc⁴⁸ in which Sara is rescued by the Sara^Ubi.PJ transgene) increase the penetrance of the Lar^bypass ISNb bypass phenotype by more than 2-fold.

(Fox and Zinn, 2005)

The midline crossing errors seen in the central nervous system of Abl²/Abl² embryos are suppressed by Lar^bypass/Lar^bypass.

(Wills et al., 2002)

The Lar^bypass/Lar^bypass and Lar^bypass/Lar^13.2 ISNb "bypass" phenotype is suppressed by Abl¹. The Lar^bypass/Lar^E55 ISNb "bypass" phenotype is suppressed by Abl¹, Abl² or Abl⁴. The ISNd bypass phenotype of Lar^bypass/Lar^E55 embryos is also suppressed by Abl¹.

(Wills et al., 1999)

Mutation causes synergy of the Scer\GAL4^elav-C155, Rac1^N17.Scer\UAS full ISNb bypass phenotype.

(Kaufmann et al., 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Shows phenotypic penetrance of approximately one-third to one-half of Lar null alleles.

(Krueger et al., 2003)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

DLAR^bypass

(Prakash et al., 2009)

Dlar^byp

(Kaufmann et al., 2002, Wills et al., 2002)

Dlar^bypass

(Krueger et al., 2003, Wills et al., 1999)

LAR^bypass

(Prakash et al., 2009)

Lar^bypass

(Hofmeyer, 2006)

lar^byp

(Fox and Zinn, 2005)

Name Synonyms

Secondary FlyBase IDs

References (10)

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