FlyBase Allele Report: Dmel\Sdc[48]

General Information

Symbol

Dmel\Sdc⁴⁸

Species

D. melanogaster

Name

FlyBase ID

FBal0159765

Feature type

allele

Associated gene

Dmel\Sdc

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Johnson et al., 2004)

Progenitor genotype

P{PZ}Sdc¹⁰⁶⁰⁸

(Johnson et al., 2004)

Caused by aberration

Df(2R)48

Cytology

Description

Imprecise excision resulting in a deletion which removes the first two exons of Sdc, including the promoter and 5' untranslated region, the translation start codon and the signal sequence.

(Johnson et al., 2004)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy

(Johnson et al., 2004)

abnormal neuroanatomy (with Sdc¹⁰⁶⁰⁸)

(Rawson et al., 2005)

abnormal neuroanatomy | larval stage (with Sdc¹⁰⁶⁰⁸), with Sara^Ubi.PJ

(Johnson et al., 2006)

abnormal neurophysiology (with Sdc¹⁰⁶⁰⁸)

(Rawson et al., 2005)

lethal (with Df(2R)Exel6067)

(Schulz et al., 2011)

partially lethal - majority die (with Sdc²³)

(Schulz et al., 2011)

partially lethal - majority die (with Sdc⁹⁷)

(Schulz et al., 2011)

partially lethal - majority die (with Sdc²⁶³⁹)

(Schulz et al., 2011)

Phenotype Manifest In

anterior fascicle & axon | ectopic

(Fox and Zinn, 2005)

eye photoreceptor cell & axon (with Sdc¹⁰⁶⁰⁸)

(Rawson et al., 2005)

lamina plexus (with Sdc¹⁰⁶⁰⁸)

(Rawson et al., 2005)

medulla (with Sdc¹⁰⁶⁰⁸)

(Rawson et al., 2005)

NMJ bouton | larval stage (with Sdc¹⁰⁶⁰⁸), with Sara^Ubi.PJ

(Johnson et al., 2006)

presumptive embryonic/larval central nervous system

(Johnson et al., 2004)

Detailed Description

Statement

Reference

Sdc¹⁰⁶⁰⁸/Df(2R)48 larvae (carrying Sara^Ubi.PJ to provide Sara function) show a significant reduction in bouton number at the neuromuscular junction compared to wild type. Mean active zone area and number of active zones per bouton are normal.

Lar^5.5/+ enhances the reduction in bouton number at the neuromuscular junction which is seen in Sdc^KG06163/Df(2R)48 larvae (carrying Sara^Ubi.PJ to provide Sara function).

(Johnson et al., 2006)

Around 8% of Sdc⁴⁸ mutants (in which Sara has been rescued by expression of the Sara^Ubi.PJ transgene) show an ISNb bypass phenotype.

(Fox and Zinn, 2005)

Half of Df(2R)48/Sdc¹⁰⁶⁰⁸ larvae (in which Sara function has been rescued by Sara^Ubi.PJ) show photoreceptor projection and lamina-plexus defects. At the pupal level, these mutants show crossover of R7 axons between medullary cartridges and defective axon pathfinding to the medulla, with a low penetrance of R7/R8 terminal disruption. Electrophysiological analysis shows that as adults, these mutants have grossly abnormal ERGs, with defective photoreceptor polarization and complete absence of on- and off-transients.

(Rawson et al., 2005)

Df(2R)48/Sdc¹⁰⁶⁰⁸ mutants (in which Sara function has been rescued by Sara^Ubi.PJ) have no rough eye phenotype and show no defects in the specification of photoreceptors, glia or lamina neurons.

(Rawson, 2005)

Df(2R)48 embryos (in which Sara function has been rescued by Sara^Ubi.PJ) show a high frequency of axons crossing the midline in the central nervous system.

(Johnson et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Sara^Ubi.PJ, Sdc⁴⁸/Sdc^KG06163 has abnormal neuroanatomy | larval stage phenotype, enhanceable by Lar^5.5/Lar[+]

(Johnson et al., 2006)

Suppressed by

Statement

Reference

Sdc⁴⁸/Sdc¹⁰⁶⁰⁸ has abnormal neuroanatomy phenotype, suppressible | partially by Scer\GAL4^elav-C155/dlp^UAS.Tag:HA

(Rawson et al., 2005)

Sdc⁴⁸ has abnormal neuroanatomy phenotype, suppressible by dlp^UAS.cBa/Scer\GAL4^elav.PLu

(Johnson et al., 2004)

NOT suppressed by

Statement

Reference

Sdc⁴⁸/Sdc¹⁰⁶⁰⁸ has abnormal neurophysiology phenotype, non-suppressible by Scer\GAL4^elav-C155/dlp^UAS.Tag:HA

(Rawson et al., 2005)

Sdc⁴⁸ has abnormal neuroanatomy phenotype, non-suppressible by dlp^UAS.cBa/Scer\GAL4^sli.PS

(Johnson et al., 2004)

Other

Statement

Reference

Sara^Ubi.PJ, Sdc⁴⁸ has lethal phenotype

(Schulz et al., 2011)

Sara^Ubi.PJ, Sdc⁴⁸/Sdc²⁶³⁹ has partially lethal - majority die phenotype

(Schulz et al., 2011)

Sara^Ubi.PJ, Sdc⁴⁸/Sdc²³ has partially lethal - majority die phenotype

(Schulz et al., 2011)

Sara^Ubi.PJ, Sdc⁹⁷/Sdc⁴⁸ has partially lethal - majority die phenotype

(Schulz et al., 2011)

Sdc⁴⁸/Df(2R)Exel6067, Sara^Ubi.PJ has lethal phenotype

(Schulz et al., 2011)

Sdc⁴⁸/Df(2R)PK1, Sara^Ubi.PJ has lethal phenotype

(Schulz et al., 2011)

Sdc⁴⁸/Df(2R)PI12, Sara^Ubi.PJ has partially lethal - majority live phenotype

(Schulz et al., 2011)

Df(2R)XE-2900/Sdc⁴⁸, Sara^Ubi.PJ has viable phenotype

(Schulz et al., 2011)

Phenotype Manifest In

Enhanced by

Statement

Reference

Sara^Ubi.PJ, Sdc⁴⁸/Sdc^KG06163 has NMJ bouton | larval stage phenotype, enhanceable by Lar^5.5/Lar[+]

(Johnson et al., 2006)

Suppressed by

Statement

Reference

Sdc⁴⁸/Sdc¹⁰⁶⁰⁸ has medulla phenotype, suppressible by Scer\GAL4^elav-C155/dlp^UAS.Tag:HA

(Rawson et al., 2005)

Sdc⁴⁸ has presumptive embryonic/larval central nervous system phenotype, suppressible by dlp^UAS.cBa/Scer\GAL4^elav.PLu

(Johnson et al., 2004)

NOT suppressed by

Statement

Reference

Sdc⁴⁸ has presumptive embryonic/larval central nervous system phenotype, non-suppressible by dlp^UAS.cBa/Scer\GAL4^sli.PS

(Johnson et al., 2004)

Enhancer of

Statement

Reference

Sdc⁴⁸/Sdc¹⁰⁶⁰⁸ is an enhancer of anterior fascicle & axon | ectopic phenotype of Lar^5.5/Lar^13.2

(Fox and Zinn, 2005)

Sdc⁴⁸ is an enhancer of anterior fascicle & axon | ectopic phenotype of Lar^bypass

(Fox and Zinn, 2005)

Suppressor of

Statement

Reference

Sdc⁴⁸/Sdc[+] is a suppressor of wing sensillum phenotype of Hsepi^d12

(Dejima et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

A heterozygous Sdc⁴⁸ background does not result in a decreased number of chemosensory bristles in a Hsepi^d12 homozygous mutants.

(Dejima et al., 2013)

Sdc⁴⁸ (in which Sara has been rescued by expression of the Sara^Ubi.PJ transgene) increases the penetrance of the Lar^bypass ISNb bypass phenotype by more than 2-fold. Sdc⁴⁸/Sdc¹⁰⁶⁰⁸; Lar^13.2/Lar^5.5 (in which Sara has been rescued by expression of the Sara^Ubi.PJ transgene) mutants display an increased penetrance of the bypass phenotype (43% vs. 28%) relative to the corresponding Lar^13.2/Lar^5.5 transheterozygote.

(Fox and Zinn, 2005)

Expression of dlp^{Scer\UAS.T:Ivir\HA1}, under the control of Scer\GAL4^elav-C155, rescues the medulla patterning defects of Sdc¹⁰⁶⁰⁸/Df(2R)48 mutants (in which Sara function has been rescued by Sara^Ubi.PJ). However, this expression fails to rescue the ERG defects.

(Rawson et al., 2005)

Expression of dlp^Scer\UAS.cBa under the control of Scer\GAL4^sli.PS fails to rescue the axon midline crossing phenotype seen in Df(2R)48 embryos in which Sara function has been rescued by Sara^Ubi.PJ. Expression of dlp^Scer\UAS.cBa under the control of Scer\GAL4^elav.PLu significantly rescues the axon midline crossing phenotype seen in Df(2R)48 embryos in which Sara function has been rescued by Sara^Ubi.PJ.

(Johnson et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Sdc⁴⁸/Sdc¹⁰⁶⁰⁸ is rescued by Scer\GAL4^elav.PU/Sdc^UAS.cJa

(Johnson et al., 2006)

Sdc⁴⁸ is rescued by Scer\GAL4^elav.PLu/Sdc^UAS.cJa

(Johnson et al., 2004)

Partially rescued by

Sdc⁴⁸/Sdc¹⁰⁶⁰⁸ is partially rescued by Scer\GAL4^elav-C155/Sdc^UAS.cSa

(Rawson et al., 2005)

Not rescued by

Sdc⁴⁸ is not rescued by Scer\GAL4^sli.PS/Sdc^UAS.cJa

(Johnson et al., 2004)

Comments

Expression of Sdc^Scer\UAS.cJa under the control of Scer\GAL4^elav.PU almost completely rescues the reduction in bouton number at the neuromuscular junction which is seen in Sdc¹⁰⁶⁰⁸/Df(2R)48 larvae (carrying Sara^Ubi.PJ to provide Sara function).

(Johnson et al., 2006)

Expression of Sdc^Scer\UAS.cSa, under the control of Scer\GAL4^elav-C155, rescues the medulla cartridge crossover and R7/R8 termini disruption pupal phenotypes of Df(2R)48/Sdc¹⁰⁶⁰⁸ mutants (in which Sara function has been rescued by Sara^Ubi.PJ). However, this expression fails to rescue photoreceptor projection defects to the larval lamina or ERG abnormalities in adults.

(Rawson et al., 2005)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Sdc⁴⁸

sdc⁴⁸

(Dejima et al., 2013, Schulz et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (9)

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