FlyBase Allele Report: Dmel\Sdc[UAS.cJa]

General Information

Symbol

Dmel\Sdc^UAS.cJa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Johnson

FlyBase ID

FBal0159762

Feature type

allele

Associated gene

Dmel\Sdc

Associated Insertion(s)

Carried in Construct

P{UAS-Sdc.J}

Also Known As

UAS-Sdc, UAS-syndecan

Key Links

Transgenic product class

cDNA

(Johnson et al., 2004)

wild_type

(Johnson et al., 2004)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Johnson et al., 2004)

wild_type

(Johnson et al., 2004)

Mutagen

in vitro construct

(Johnson et al., 2004)

Progenitor genotype

Carried in construct

P{UAS-Sdc.J}

Cytology

Description

UAS regulatory sequences drive expression of a Sdc cDNA.

(Johnson et al., 2004)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Sdc

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^how-24B

(Fox and Zinn, 2005)

abnormal neuroanatomy | larval stage, with Scer\GAL4^elav.PU

(Johnson et al., 2006)

abnormal neuroanatomy | larval stage, with Scer\GAL4^how-24B

(Johnson et al., 2006)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^elav.PU

(Nguyen et al., 2016)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^how-24B

(Nguyen et al., 2016)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Sdc^Scer\UAS.cJa driven post-synaptically by Scer\GAL4^how-24B or presynaptically by Scer\GAL4^elav.PU leads to a significant increase in the number of boutons per neuromuscular junction, compared to controls.

(Nguyen et al., 2016)

Expression of Sdc^Scer\UAS.cJa under the control of either Scer\GAL4^how-24B or Scer\GAL4^elav.PU results in an increase in bouton number at the neuromuscular junction compared to wild type.

(Johnson et al., 2006)

Expression of Sdc^Scer\UAS.cJa, under the control of Scer\GAL4^how-24B, leads to SNa bifurcation with a penetrance of 18%. No ISNb phenotype is observed.

(Fox and Zinn, 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^how-24B, Sdc^UAS.cJa has abnormal neuroanatomy phenotype, suppressible by Lar^5.5/Lar^13.2

(Fox and Zinn, 2005)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^how-24B, Sdc^UAS.cJa has larval abdominal segmental nerve phenotype, suppressible by Lar^5.5/Lar^13.2

(Fox and Zinn, 2005)

Additional Comments

Genetic Interactions

Statement

Reference

Sdc¹⁰⁶⁰⁸/Df(2R)48 third instar larvae (in which Sara function has been rescued by Sara^Ubi.PJ) have a decrease in the number of boutons per neuromuscular junction, compared to controls; this phenotype is completely rescued by pre- (Scer\GAL4^elav.PU) or post- (Scer\GAL4^how-24B) synaptic expression of Sdc^{Scer\UAS.FL.T:Hsap\MYC}, or pre- (and partial rescue with post-) synaptic expression of Sdc^Scer\UAS.cJa.

(Nguyen et al., 2016)

Larvae co-expressing Sdc^Scer\UAS.cJa and dlp^Scer\UAS.cBa under the control of Scer\GAL4^how-24B show a reduction in bouton number at the neuromuscular junction compared to wild type, as occurs in larvae expressing dlp^Scer\UAS.cBa alone under the control of Scer\GAL4^how-24B.

(Johnson et al., 2006)

When Sdc^Scer\UAS.cJa is expressed, under the control of Scer\GAL4^how-24B, in a Lar^13.2/Lar^5.5 background, the SNa bifurcation phenotype seen when the transgene is expressed in a wild-type background is suppressed.

(Fox and Zinn, 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^elav.PU/Sdc^UAS.cJa rescues Sdc¹⁰⁶⁰⁸/Df(2R)48

(Nguyen et al., 2016)

Scer\GAL4^elav.PU/Sdc^UAS.cJa rescues Sdc⁴⁸/Sdc¹⁰⁶⁰⁸

(Johnson et al., 2006)

Scer\GAL4^elav.PLu/Sdc^UAS.cJa rescues Sdc⁴⁸

(Johnson et al., 2004)

Partially rescues

Scer\GAL4^how-24B/Sdc^UAS.cJa partially rescues Sdc¹⁰⁶⁰⁸/Df(2R)48

(Nguyen et al., 2016)

Scer\GAL4^how-24B/Sdc^UAS.cJa partially rescues Sdc¹⁰⁶⁰⁸

(Johnson et al., 2006)

Fails to rescue

Scer\GAL4^sli.PS/Sdc^UAS.cJa fails to rescue Sdc⁴⁸

(Johnson et al., 2004)

Comments

Sdc¹⁰⁶⁰⁸/Df(2R)48 third instar larvae (in which Sara function has been rescued by Sara^Ubi.PJ) have a decrease in the number of boutons per neuromuscular junction, compared to controls; this phenotype is completely rescued by pre- (Scer\GAL4^elav.PU) and partial rescue with post- (Scer\GAL4^how-24B) synaptic expression of Sdc^Scer\UAS.cJa.

(Nguyen et al., 2016)

Expression of Sdc^Scer\UAS.cJa under the control of Scer\GAL4^elav.PU almost completely rescues the reduction in bouton number at the neuromuscular junction which is seen in Sdc¹⁰⁶⁰⁸/Df(2R)48 larvae (carrying Sara^Ubi.PJ to provide Sara function).

Expression of Sdc^Scer\UAS.cJa under the control of Scer\GAL4^how-24B provides limited rescue of the reduction in bouton number at the neuromuscular junction which is seen in homozygous Sdc¹⁰⁶⁰⁸ larvae.

(Johnson et al., 2006)

Expression of Sdc^Scer\UAS.cJa under the control of Scer\GAL4^sli.PS fails to rescue the axon midline crossing phenotype seen in Df(2R)48 embryos in which Sara function has been rescued by Sara^Ubi.PJ. Expression of Sdc^Scer\UAS.cJa under the control of Scer\GAL4^elav.PLu rescues the axon midline crossing phenotype seen in Df(2R)48 embryos in which Sara function has been rescued by Sara^Ubi.PJ.

(Johnson et al., 2004)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

8564

y¹ w^*; P{UAS-Sdc.J}3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Sdc^Scer\UAS.cJa

Sdc^UAS.cJa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Johnson

(Johnson et al., 2004)

Secondary FlyBase IDs

References (9)

Report Sections

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