FlyBase Allele Report: Dmel\Lar[2127]

General Information

Symbol

Dmel\Lar²¹²⁷

Species

D. melanogaster

Name

FlyBase ID

FBal0128174

Feature type

allele

Associated gene

Dmel\Lar

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Maurel-Zaffran et al., 2001)

Mutagen

ethyl methanesulfonate

(Maurel-Zaffran et al., 2001)

Progenitor genotype

Cytology

Description

Amino acid replacement: W834term.

(Maurel-Zaffran et al., 2001)

Nucleotide substitution: G?A.

(Maurel-Zaffran et al., 2001)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:19,726,369..19,726,369 [+]

Nucleotide change:

G19726369A

Reported nucleotide change:

G?A

Amino acid change:

Reported amino acid change:

W834term

Comment:

G to A nucleotide change at the second or third position of the wild type Trp codon leads to a nonsense mutation (exact site of mutation unspecified). The mutation was annotated at the second base of the codon.

(Maurel-Zaffran et al., 2001)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

axon (with Lar^C12)

(Cameron et al., 2013)

axon | pupal stage | somatic clone

(Özel et al., 2019)

eye disc | third instar larval stage (with Lar^C12)

(Hofmeyer and Treisman, 2009)

growth cone filopodium | pupal stage | somatic clone

(Özel et al., 2019)

NMJ bouton | third instar larval stage (with Lar^C12)

(Hofmeyer and Treisman, 2009)

optic cartridge

(Choe et al., 2006)

photoreceptor cell R4 | pupal stage | somatic clone

(Prakash et al., 2009)

photoreceptor cell R7

(Nern et al., 2008, Choe et al., 2006)

photoreceptor cell R7 (with Lar^bypass)

(Hofmeyer et al., 2006)

photoreceptor cell R7 (with Lar^C12)

(Hofmeyer et al., 2006)

photoreceptor cell R7 & axon (with Lar^5.5)

(Maurel-Zaffran et al., 2001)

photoreceptor cell R7 & axon (with Lar^C12)

(Maurel-Zaffran et al., 2001)

photoreceptor cell R7 & axon | somatic clone

(Clandinin et al., 2001)

photoreceptor cell R7 & growth cone (with Lar^5.5)

(Maurel-Zaffran et al., 2001)

photoreceptor cell R7 & growth cone | somatic clone

(Ting et al., 2005)

photoreceptor cell R7 | pupal stage | somatic clone

(Özel et al., 2019)

photoreceptor cell R7 | somatic clone

(Holbrook et al., 2012)

photoreceptor cell R7 | third instar larval stage (with Lar^C12)

(Hofmeyer and Treisman, 2009)

photoreceptor neuron | cell autonomous | pupal stage | somatic clone

(Prakash et al., 2009)

rhabdomere R7 (with Lar^C12)

(Cameron et al., 2013)

Detailed Description

Statement

Reference

Lar²¹²⁷ homozygous MARCM clones of R7 photoreceptor neurons at 40-60 percent through pupal development (P40-60) have significantly reduced lifespan of bulbous growth cone filopodia (bulbs) at axon terminals, with no change in the number of transient bulbs, but significantly fewer stable bulbs and total bulbs, compared to controls, with no effect on other filopodia; unlike controls there are many timepoints during P60 where no bulbs are present; axons begin to retract at P40 and most are retracted by P70, unlike controls, which do not retract.

(Özel et al., 2019)

In Lar^C12/Lar²¹²⁷ mutants, many R7 axons are mis-targeted in the retina.

(Cameron et al., 2013)

Axon terminals of homozygous R7 photoreceptor cell clones often fail to contact the M6 layer of the medulla.

(Holbrook et al., 2012)

Most R7 photoreceptor axons terminate at the more superficial R8 layer in Lar^C12/Lar²¹²⁷ mutant eye discs.

Reduced synaptic growth at the neuromuscular junction is observed in Lar^C12/Lar²¹²⁷ mutant third instar larvae.

(Hofmeyer and Treisman, 2009)

57% of pupal R1-R6 axons mutant for Lar²¹²⁷ do not extend their axons, or show aberrant axon extension, targeting, and morphology.

Pupal R4 axons frequently fail to extend, or extend aberrantly when mutant for Lar²¹²⁷.

(Prakash et al., 2009)

Using MARCM, Lar²¹²⁷ mutant lamina neurons do not show mistargeting phenotypes in lamina cells L1-L5, whilst 95% of mutant R7 cells show mistargeting.

(Nern et al., 2008)

Lar²¹²⁷ mutants exhibit a specific pattern of disruption in the structure of the cartridge, the synaptic unit in the lamina. Some cartridges have either >6 or <6 R cells axons, and some adjacent cartridges fuse.

In Lar²¹²⁷ mutants, R7 axons frequently stop at abnormally distal positions within the R8 recipient layer, leaving gaps in the array of otherwise regular R7 termini. However the ganglion-specific targeting of R1-R6 axons to the lamina nor the layer-specific targeting of R8 axons within the medulla are affected.

(Choe et al., 2006)

Lar²¹²⁷/Lar^C12 mutants exhibit aberrant R7 photoreceptor targeting, where approximately 15% of R7 axons project beyond the R8 layer. R1-R6 targeting is as wild-type.

In Lar²¹²⁷/Lar^bypass mutants, wild-type targeting of R7 photoreceptor cells occurs in approximately 20% of cases.

(Hofmeyer et al., 2006)

Stage 14 oocytes from Lar²¹²⁷/Lar^C12 mutants are defective in egg elongation in 30% of cases.

Stage 14 oocytes from Lar²¹²⁷/Lar^bypass females are indistinguishable from wild-type.

(Hofmeyer, 2006)

Lar²¹²⁷ R7 axons (analysed as single cell mutant clones) target correctly in the medulla at both 17% and 35% APF, although some growth cones have a collapsed morphology.

(Ting et al., 2005)

Mosaic flies in which the photoreceptor cells are homozygous show defects in the optomotor response (75% fail to respond to a motion stimulus) and the UV/visual light choice test (69% show phototaxis towards visible light, compared to only 12% of wild-type flies). 74% of single homozygous R7 photoreceptor cells in the lamina (induced in a heterozygous background) fail to terminate at their normal target layer. Mosaic animals in which the whole eye is homozygous show 81% mistargeted R7 axons.

(Clandinin et al., 2001)

Homozygous clones in the eye contain the normal arrangement of photoreceptors. R7 growth cones extend beyond the R8 termini in Lar²¹²⁷/Lar^5.5 animals 15 hours after pupariation as occurs in wild type, although the R7 growth cones have a compact, club-like shape instead of the normal expanded morphology. Only 52.9% of Lar²¹²⁷/Lar^5.5 R7 axons select their correct target layer.

(Maurel-Zaffran et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Lar²¹²⁷/Lar⁴⁵¹ has lethal phenotype, enhanceable by Liprin-α^E/Liprin-alpha[+]

(Prakash et al., 2009)

Lar²¹²⁷/Lar⁴⁵¹ has lethal phenotype, enhanceable by CadN[+]/CadN^Δ14

(Prakash et al., 2009)

Lar²¹²⁷ has abnormal neuroanatomy | pupal stage | cell autonomous | somatic clone phenotype, enhanceable by Scer\GAL4^elav-C155/CadN^UAS.cIa

(Prakash et al., 2009)

Suppressed by

Statement

Reference

Lar^C12/Lar²¹²⁷ has abnormal neuroanatomy phenotype, suppressible by bdl^EX2/bdl[+]

(Cameron et al., 2013)

Lar²¹²⁷ has abnormal neuroanatomy | somatic clone | adult stage phenotype, suppressible by Scer\GAL4^Act.PU/trio^UAS.cBa

(Holbrook et al., 2012)

Lar²¹²⁷ has abnormal neuroanatomy | somatic clone | adult stage phenotype, suppressible by Scer\GAL4^Act.PU/RhoGAP100F^UAS.Tag:FLAG

(Holbrook et al., 2012)

Lar^C12/Lar²¹²⁷ has abnormal neuroanatomy phenotype, suppressible by Scer\GAL4^GMR.PF/Liprin-α^UAS.Tag:HA

(Hofmeyer et al., 2006)

Suppressor of

Statement

Reference

Lar²¹²⁷/Lar[+] is a suppressor | partially of lethal phenotype of Liprin-α^E/Liprin-α¹

(Prakash et al., 2009)

Lar²¹²⁷/Lar[+] is a suppressor | partially of lethal phenotype of Liprin-α^F/Liprin-α^E

(Prakash et al., 2009)

Other

Statement

Reference

CadN[+]/CadN^Δ14, Lar²¹²⁷/Lar[+], Liprin-α^F has partially lethal - majority die | dominant phenotype

(Prakash et al., 2009)

Phenotype Manifest In

Enhanced by

Statement

Reference

Lar²¹²⁷ has photoreceptor neuron | pupal stage | cell autonomous | somatic clone phenotype, enhanceable by Scer\GAL4^elav-C155/CadN^UAS.cIa

(Prakash et al., 2009)

Lar^5.5/Lar²¹²⁷ has photoreceptor cell R7 & axon phenotype, enhanceable by ena[+]/ena^GC1

(Maurel-Zaffran et al., 2001)

Lar^5.5/Lar²¹²⁷ has photoreceptor cell R7 & axon phenotype, enhanceable by trio¹/trio[+]

(Maurel-Zaffran et al., 2001)

Suppressed by

Statement

Reference

Lar^C12/Lar²¹²⁷ has axon phenotype, suppressible by bdl^EX2/bdl[+]

(Cameron et al., 2013)

Lar^C12/Lar²¹²⁷ has rhabdomere R7 phenotype, suppressible by bdl^EX2/bdl[+]

(Cameron et al., 2013)

Lar²¹²⁷ has photoreceptor cell R7 | somatic clone phenotype, suppressible by Scer\GAL4^Act.PU/trio^UAS.cBa

(Holbrook et al., 2012)

Lar²¹²⁷ has photoreceptor cell R7 | somatic clone phenotype, suppressible by Scer\GAL4^Act.PU/RhoGAP100F^UAS.Tag:FLAG

(Holbrook et al., 2012)

Lar^C12/Lar²¹²⁷ has photoreceptor cell R7 phenotype, suppressible by Scer\GAL4^GMR.PF/Liprin-α^UAS.Tag:HA

(Hofmeyer et al., 2006)

Lar^5.5/Lar²¹²⁷ has photoreceptor cell R7 & axon phenotype, suppressible by trio^GMR.PN

(Maurel-Zaffran et al., 2001)

Lar^C12/Lar²¹²⁷ has photoreceptor cell R7 & axon phenotype, suppressible | partially by Scer\GAL4^Pan-R7/ena^{UAS.Tag:polyHis}

(Maurel-Zaffran et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

bdl^EX2/+ significantly increases the number of correctly targeted R7 axons in Lar^C12/Lar²¹²⁷ mutants.

(Cameron et al., 2013)

Expression of either trio^Scer\UAS.cBa or RhoGAP100F^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^Act.PU in Lar²¹²⁷ R7 photoreceptor cell clones partially suppresses the failure of the axon terminals to contact the M6 layer of the medulla.

(Holbrook et al., 2012)

Expression of CadN^Scer\UAS.cIa under the control of Scer\GAL4^elav-C155 in Lar²¹²⁷ somatic mutant clones enhances the photoreceptor targeting defects observed in single mutants.

When large clones of cells mutant for both Lar²¹²⁷ and CadN^Δ14 are generated in the eye, R4 axon targeting errors are more frequently observed than in single mutants.

When large clones of cells mutant for both Liprin-α^E and Lar²¹²⁷ are generated in the eye, R4 axon targeting errors are more frequently observed than in single mutants.

When large clones of cells mutant for Liprin-α^E, Lar²¹²⁷, and CadN^Δ14 are generated in the eye, R4 axon targeting errors are observed, but the frequency of defects is similar to Liprin-α^E; Lar²¹²⁷, Lar²¹²⁷; CadN^Δ14, or Liprin-α^E; CadN^Δ14 double mutant combinations.

(Prakash et al., 2009)

Overexpression of Liprin-α^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^elav-C155 or Scer\GAL4^GMR.PF in a Lar²¹²⁷/Lar^C12 mutant background partially restores R7 targeting.

(Hofmeyer et al., 2006)

CadN⁴⁰⁵ Lar²¹²⁷ double mutant R7 growth cones show similar phenotypes to CadN⁴⁰⁵ single mutant R7 growth cones.

(Ting et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Lar^unspecified

(Maurel-Zaffran et al., 2001)

Rescued by

Lar^C12/Lar²¹²⁷ is rescued by Scer\GAL4^elav-C155/Lar^UAS.Tag:HA

(Hofmeyer and Treisman, 2009)

Lar^C12/Lar²¹²⁷ is rescued by Scer\GAL4^elav-C155/Lar^ΔIg123.UAS

(Hofmeyer and Treisman, 2009)

Lar^C12/Lar²¹²⁷ is rescued by Scer\GAL4^elav-C155/Lar^ΔFn123.UAS

(Hofmeyer and Treisman, 2009)

Lar^C12/Lar²¹²⁷ is rescued by Scer\GAL4^elav-C155/Lar^ΔFn456.UAS

(Hofmeyer and Treisman, 2009)

Lar^C12/Lar²¹²⁷ is rescued by Scer\GAL4^elav-C155/Lar^C1929S.UAS

(Hofmeyer and Treisman, 2009)

Lar^C12/Lar²¹²⁷ is rescued by Scer\GAL4^elav-C155/Lar^{ΔIg123ΔFn1-6.UAS.Tag:SS(wg),Tag:HA}

(Hofmeyer and Treisman, 2009)

Lar²¹²⁷ is rescued by Scer\GAL4^elav-C155/Lar^UAS.cKa

(Prakash et al., 2009)

Lar²¹²⁷ is rescued by Scer\GAL4^elav-C155/Lar^CSX2.UAS

(Prakash et al., 2009)

Lar²¹²⁷ is rescued by Scer\GAL4^elav-C155/Lar^C1929S.UAS

(Prakash et al., 2009)

Lar^C12/Lar²¹²⁷ is rescued by Scer\GAL4^elav-C155/Lar^UAS.Tag:HA

(Hofmeyer et al., 2006)

Lar^C12/Lar²¹²⁷ is rescued by Lar^UAS.Tag:HA

(Hofmeyer, 2006)

Lar^5.5/Lar²¹²⁷ is rescued by Lar^UAS.cKa/Scer\GAL4^hs.2sev

(Maurel-Zaffran et al., 2001)

Partially rescued by

Lar^C12/Lar²¹²⁷ is partially rescued by Scer\GAL4^elav-C155/Lar^CSX2.UAS

(Hofmeyer and Treisman, 2009)

Lar^C12/Lar²¹²⁷ is partially rescued by Scer\GAL4^elav-C155/Lar^C1638S.UAS

(Hofmeyer and Treisman, 2009)

Lar^C12/Lar²¹²⁷ is partially rescued by Scer\GAL4^elav-C155/Lar^D1YLCS.UAS

(Hofmeyer and Treisman, 2009)

Lar^C12/Lar²¹²⁷ is partially rescued by Scer\GAL4^elav-C155/Lar^PPLL.UAS

(Hofmeyer and Treisman, 2009)

Lar^C12/Lar²¹²⁷ is partially rescued by Scer\GAL4^elav-C155/Lar^{PPLL.D1CS.UAS}

(Hofmeyer and Treisman, 2009)

Lar²¹²⁷ is partially rescued by Scer\GAL4^elav-C155/Lar^ΔIg123.UAS

(Prakash et al., 2009)

Lar²¹²⁷ is partially rescued by Scer\GAL4^elav-C155/Lar^ΔFn2-9.UAS

(Prakash et al., 2009)

Lar^5.5/Lar²¹²⁷ is partially rescued by Lar^UAS.cKa/Scer\GAL4^sca-109-68

(Maurel-Zaffran et al., 2001)

Lar^5.5/Lar²¹²⁷ is partially rescued by Lar^{ΔC.UAS.Tag:SS(wg),Tag:HA}/Scer\GAL4^sca-109-68

(Maurel-Zaffran et al., 2001)

Not rescued by

Lar^C12/Lar²¹²⁷ is not rescued by Scer\GAL4^elav-C155/Lar^ΔFn789.UAS

(Hofmeyer and Treisman, 2009)

Lar^C12/Lar²¹²⁷ is not rescued by Scer\GAL4^elav-C155/Lar^ΔFn2-9.UAS

(Hofmeyer and Treisman, 2009)

Lar^C12/Lar²¹²⁷ is not rescued by Scer\GAL4^elav-C155/Lar^ΔPTP-D2.UAS

(Hofmeyer et al., 2006)

Lar^C12/Lar²¹²⁷ is not rescued by Scer\GAL4^T155/Lar^{ΔC.UAS.Tag:SS(wg),Tag:HA}

(Hofmeyer, 2006)

Lar^5.5/Lar²¹²⁷ is not rescued by Scer\GAL4^hs.2sev/Lar^{ΔC.UAS.Tag:SS(wg),Tag:HA}

(Maurel-Zaffran et al., 2001)

Comments

Expression of Lar^{Scer\UAS.T:Ivir\HA1}, Lar^{C1638S.Scer\UAS}, Lar^{C1929S.Scer\UAS}, Lar^{CSX2.Scer\UAS}, Lar^{D1YLCS.Scer\UAS}, Lar^{ΔIg123.Scer\UAS}, Lar^{ΔFn123.Scer\UAS}, Lar^{ΔFn456.Scer\UAS}, or Lar^{ΔIg123ΔFn1-6.Scer\UAS.T:wg,T:Ivir\HA1} under the control of Scer\GAL4^elav-C155 in Lar^C12/Lar²¹²⁷ mutant eye discs rescues axon mistargeting. Expression of Lar^{PPLL.Scer\UAS} or Lar^{PPLL.D1CS.Scer\UAS} only partially rescues the axon targeting phenotype. Expression of Lar^{ΔFn789.Scer\UAS} or Lar^{ΔFn2-9.Scer\UAS} fails to rescue the axon targeting phenotype.

Expression of Lar^{Scer\UAS.T:Ivir\HA1} or Lar^{PPLL.Scer\UAS} under the control of Scer\GAL4^elav-C155 in Lar^C12/Lar²¹²⁷ mutant third instar larvae rescues synaptic growth at the neuromuscular junction. Expression of Lar^{C1638S.Scer\UAS}, Lar^{D1YLCS.Scer\UAS}, or Lar^{CSX2.Scer\UAS} only partially rescues this phenotype.

(Hofmeyer and Treisman, 2009)

Expression of Lar^Scer\UAS.cKa under the control of Scer\GAL4^elav-C155 almost completely rescues the aberrant axon extension in Lar²¹²⁷ mutant photoreceptor cells.

Expression of Lar^{CSX2.Scer\UAS} under the control of Scer\GAL4^elav-C155 almost completely rescues the aberrant axon extension in Lar²¹²⁷ mutant photoreceptor cells.

Expression of Lar^{C1929S.Scer\UAS} under the control of Scer\GAL4^elav-C155 completely rescues the aberrant axon extension in Lar²¹²⁷ mutant photoreceptor cells.

Expression of Lar^{ΔIg123.Scer\UAS} under the control of Scer\GAL4^elav-C155 partially rescues the aberrant axon extension in Lar²¹²⁷ mutant photoreceptor cells.

Expression of Lar^{ΔFn2-9.Scer\UAS} under the control of Scer\GAL4^elav-C155 partially rescues the aberrant axon extension in Lar²¹²⁷ mutant photoreceptor cells.

(Prakash et al., 2009)

Expression of Lar^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^elav-C155 rescues R7 targeting in Lar²¹²⁷/Lar^C12 mutants.

Expression of Lar^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^elav-C155 fails to rescue R7 targeting in Lar²¹²⁷/Lar^C12 mutants.

(Hofmeyer et al., 2006)

Expression of Lar^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^T155 rescues the egg elongation phenotype observed in stage 14 oocytes from Lar²¹²⁷/Lar^C12 mutants.

Expression of Lar^{ΔC.Scer\UAS.T:wg,T:Ivir\HA1} under the control of Scer\GAL4^T155 fails to rescue the egg elongation phenotype observed in stage 14 oocytes from Lar²¹²⁷/Lar^C12 mutants and indeed exacerbates the phenotype, with approximately 44% of the oocytes displaying a 'rounded phenotype', compared to approximately 30% in the Lar²¹²⁷/Lar^C12 mutant.

(Hofmeyer, 2006)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Selected as: a mutation that results in photoreceptor projection defects in mosaic animals in which the retina, but no other tissue is homozygous.

(Maurel-Zaffran et al., 2001)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

DLAR²¹²⁷

(Prakash et al., 2009)

Dlar^2-127

(Nern et al., 2008)

LAR²¹²⁷

(Cameron et al., 2013, Holbrook et al., 2012, Prakash et al., 2009, Choe et al., 2006, Ting et al., 2005, Clandinin et al., 2001, Maurel-Zaffran et al., 2001)

Lar²¹²⁷

(Gontang et al., 2011, Astigarraga et al., 2010, Hofmeyer and Treisman, 2009, Hofmeyer, 2006, Hofmeyer et al., 2006)

lar²¹²⁷

(Zang and Bashaw, 2023, Özel et al., 2019, Gontang et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (16)

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