FB2024_03 , released June 25, 2024
Allele: Dmel\bdlEX2
Open Close
General Information
Symbol
Dmel\bdlEX2
Species
D. melanogaster
Name
FlyBase ID
FBal0301364
Feature type
allele
Associated gene
Dmel\bdl
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Cameron et al., 2013)
Mutagen
P-element activity
(Cameron et al., 2013)
Progenitor genotype
P{SUPor-P}KG06404
(Cameron et al., 2013)
Cytology
Description

Imprecise excision of P{SUPor-P}KG06404 resulting in the deletion of the sequence encoding amino acids 317-719 of bdl.

(Cameron et al., 2013)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
2L:4,329,729..4,332,075 [+]
Comment:

Inferred boundaries of the bdlEX2 deletion resulting from the imprecise excision of P{SUPor-P}KG06404, which removes amino acids 317-719 of bdl (from aa317 to the end of the protein).

(Cameron et al., 2013)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Third instar eye discs of bdlEX2/bdlEX2 mutants do not show significantly different numbers of wrapping glia or total glia, and photoreceptor cell axons project normally into the optic lobe, but wrapping glia exhibit significantly shorter processes, with fewer processes present in the lamina, as compared to wild type. These mutants also exhibit defects in photoreceptor-cell bundle ensheathment by wrapping glia in the optic stalk.

      (Cameron et al., 2016)

      Neither synaptic-layer selection of R1-R6, R7, and R8 axons, nor tiling of R7 and R8 axons, is affected in bdlEX2 mutants.

      (Cameron et al., 2013)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Enhancer of
      Statement
      Reference

      bdlEX2/bdl[+] is a non-enhancer of abnormal neuroanatomy | third instar larval stage phenotype of Scer\GAL4Ntan1-Mz97, htlGD84

      (Cameron et al., 2016)
      Suppressor of
      Statement
      Reference

      bdlEX2/bdl[+] is a suppressor of abnormal neuroanatomy phenotype of LarC12/Lar2127

      (Cameron et al., 2013)

      bdlEX2 is a suppressor of abnormal neuroanatomy phenotype of tutl23

      (Cameron et al., 2013)

      bdlΔIg1-2.UAS, bdlEX2, Scer\GAL4GMR.long is a suppressor of abnormal neuroanatomy phenotype of tutl23

      (Cameron et al., 2013)
      NOT Suppressor of
      Phenotype Manifest In
      NOT Enhancer of
      Suppressor of
      Statement
      Reference

      bdlEX2 is a suppressor of rhabdomere R7 phenotype of tutl23

      (Cameron et al., 2013)

      bdlEX2 is a suppressor of axon phenotype of tutl23

      (Cameron et al., 2013)

      bdlΔIg1-2.UAS, bdlEX2, Scer\GAL4GMR.long is a suppressor of rhabdomere R7 phenotype of tutl23

      (Cameron et al., 2013)

      bdlΔIg1-2.UAS, bdlEX2, Scer\GAL4GMR.long is a suppressor of axon phenotype of tutl23

      (Cameron et al., 2013)

      bdlEX2/bdl[+] is a suppressor of axon phenotype of LarC12/Lar2127

      (Cameron et al., 2013)

      bdlEX2/bdl[+] is a suppressor of rhabdomere R7 phenotype of LarC12/Lar2127

      (Cameron et al., 2013)
      NOT Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      bdlEX2/+ or co-expression of bdlScer\UAS.T:Zzzz\FLAG does not modify the wrapping glial extension phenotype seen in third instar larvae expressing htlGD84 under the control of Scer\GAL4Mz97.

      (Cameron et al., 2016)

      The R7 tiling pattern in homozygous tutl23, bdlEX2 mutants is normal. This effect appears to be R-cell-specific, as similar suppression is observed when bdl is selectively removed in homozygous tutl R cells by performing eye-specific mosaic analysis.

      bdlEX2/+ significantly increases the number of correctly targeted R7 axons in LarC12/Lar2127 mutants.

      (Cameron et al., 2013)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      bdlEX2 is rescued by bdlUAS.Tag:FLAG

      (Cameron et al., 2016)
      Comments

      Expression of bdlScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4Mz97 in third instar larvae rescues the wrapping glial extension defects seen in bdlEX2/bdlEX2 mutants.

      (Cameron et al., 2016)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      bdlEX2
      (Shaw et al., 2019, Chen et al., 2017, Cameron et al., 2016, Cameron et al., 2013)
      Name Synonyms
      Secondary FlyBase IDs
        References (4)