FlyBase Allele Report: Dmel\Lar[C12]

General Information

Symbol

Dmel\Lar^C12

Species

D. melanogaster

Name

FlyBase ID

FBal0128171

Feature type

allele

Associated gene

Dmel\Lar

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Maurel-Zaffran et al., 2001)

Mutagen

ethyl methanesulfonate

(Maurel-Zaffran et al., 2001)

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy (with Lar²¹²⁷)

(Cameron et al., 2013, Hofmeyer et al., 2006)

abnormal neuroanatomy | somatic clone

(Hofmeyer et al., 2006)

abnormal neuroanatomy | third instar larval stage (with Lar²¹²⁷)

(Hofmeyer and Treisman, 2009)

abnormal optomotor response | somatic clone

(Clandinin et al., 2001)

abnormal visual behavior | somatic clone

(Clandinin et al., 2001)

Phenotype Manifest In

axon (with Lar²¹²⁷)

(Cameron et al., 2013)

eye disc | third instar larval stage (with Lar²¹²⁷)

(Hofmeyer and Treisman, 2009)

NMJ bouton | third instar larval stage (with Lar²¹²⁷)

(Hofmeyer and Treisman, 2009)

photoreceptor cell R7 (with Lar²¹²⁷)

(Hofmeyer et al., 2006)

photoreceptor cell R7 & axon (with Lar²¹²⁷)

(Maurel-Zaffran et al., 2001)

photoreceptor cell R7 | somatic clone

(Hofmeyer et al., 2006)

photoreceptor cell R7 | third instar larval stage (with Lar²¹²⁷)

(Hofmeyer and Treisman, 2009)

rhabdomere R7 (with Lar²¹²⁷)

(Cameron et al., 2013)

Detailed Description

Statement

Reference

In Lar^C12/Lar²¹²⁷ mutants, many R7 axons are mis-targeted in the retina.

(Cameron et al., 2013)

Most R7 photoreceptor axons terminate at the more superficial R8 layer in Lar^C12/Lar²¹²⁷ mutant eye discs.

Reduced synaptic growth at the neuromuscular junction is observed in Lar^C12/Lar²¹²⁷ mutant third instar larvae.

(Hofmeyer and Treisman, 2009)

Lar²¹²⁷/Lar^C12 mutants exhibit aberrant R7 photoreceptor targeting, where approximately 15% of R7 axons project beyond the R8 layer. R1-R6 targeting is as wild-type.

(Hofmeyer et al., 2006)

Stage 14 oocytes from Lar²¹²⁷/Lar^C12 mutants are defective in egg elongation.

(Hofmeyer, 2006)

Mosaic flies in which the photoreceptor cells are homozygous show defects in the optomotor response (58% fail to respond to a motion stimulus) and the UV/visual light choice test (43% show phototaxis towards visible light, compared to only 12% of wild-type flies).

(Clandinin et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Lar^C12 has abnormal neuroanatomy | somatic clone phenotype, enhanceable by Liprin-α^oos

(Hofmeyer et al., 2006)

Suppressed by

Statement

Reference

Lar^C12/Lar²¹²⁷ has abnormal neuroanatomy phenotype, suppressible by bdl^EX2/bdl[+]

(Cameron et al., 2013)

Lar^C12/Lar²¹²⁷ has abnormal neuroanatomy phenotype, suppressible by Scer\GAL4^GMR.PF/Liprin-α^UAS.Tag:HA

(Hofmeyer et al., 2006)

Enhancer of

Statement

Reference

Lar^C12 is an enhancer of abnormal neuroanatomy | somatic clone phenotype of Liprin-α^oos

(Hofmeyer et al., 2006)

Phenotype Manifest In

Enhanced by

Statement

Reference

Lar^C12 has photoreceptor cell R7 | somatic clone phenotype, enhanceable by Liprin-α^oos

(Hofmeyer et al., 2006)

Suppressed by

Statement

Reference

Lar^C12/Lar²¹²⁷ has axon phenotype, suppressible by bdl^EX2/bdl[+]

(Cameron et al., 2013)

Lar^C12/Lar²¹²⁷ has rhabdomere R7 phenotype, suppressible by bdl^EX2/bdl[+]

(Cameron et al., 2013)

Lar^C12/Lar²¹²⁷ has photoreceptor cell R7 phenotype, suppressible by Scer\GAL4^GMR.PF/Liprin-α^UAS.Tag:HA

(Hofmeyer et al., 2006)

Lar^C12/Lar²¹²⁷ has photoreceptor cell R7 & axon phenotype, suppressible | partially by Scer\GAL4^Pan-R7/ena^{UAS.Tag:polyHis}

(Maurel-Zaffran et al., 2001)

Enhancer of

Statement

Reference

Lar^C12 is an enhancer of photoreceptor cell R7 | somatic clone phenotype of Liprin-α^oos

(Hofmeyer et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Overexpression of Liprin-α^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^elav-C155 or Scer\GAL4^GMR.PF in a Lar²¹²⁷/Lar^C12 mutant background partially restores R7 targeting.

Liprin-α^oos Lar^C12 somatic clones exhibit defects in R7 targeting in over 80% of cases. This is a statistically significant difference to wild-type. They have fewer correctly targeted R7 axons than clones homozygous for Lar^C12.

(Hofmeyer et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Lar^unspecified

(Maurel-Zaffran et al., 2001)

Rescued by

Lar^C12/Lar²¹²⁷ is rescued by Scer\GAL4^elav-C155/Lar^UAS.Tag:HA

(Hofmeyer and Treisman, 2009)

Lar^C12/Lar²¹²⁷ is rescued by Scer\GAL4^elav-C155/Lar^ΔIg123.UAS

(Hofmeyer and Treisman, 2009)

Lar^C12/Lar²¹²⁷ is rescued by Scer\GAL4^elav-C155/Lar^ΔFn123.UAS

(Hofmeyer and Treisman, 2009)

Lar^C12/Lar²¹²⁷ is rescued by Scer\GAL4^elav-C155/Lar^ΔFn456.UAS

(Hofmeyer and Treisman, 2009)

Lar^C12/Lar²¹²⁷ is rescued by Scer\GAL4^elav-C155/Lar^C1929S.UAS

(Hofmeyer and Treisman, 2009)

Lar^C12/Lar²¹²⁷ is rescued by Scer\GAL4^elav-C155/Lar^{ΔIg123ΔFn1-6.UAS.Tag:SS(wg),Tag:HA}

(Hofmeyer and Treisman, 2009)

Lar^C12/Lar²¹²⁷ is rescued by Scer\GAL4^elav-C155/Lar^UAS.Tag:HA

(Hofmeyer et al., 2006)

Lar^C12/Lar²¹²⁷ is rescued by Lar^UAS.Tag:HA

(Hofmeyer, 2006)

Partially rescued by

Lar^C12/Lar²¹²⁷ is partially rescued by Scer\GAL4^elav-C155/Lar^CSX2.UAS

(Hofmeyer and Treisman, 2009)

Lar^C12/Lar²¹²⁷ is partially rescued by Scer\GAL4^elav-C155/Lar^C1638S.UAS

(Hofmeyer and Treisman, 2009)

Lar^C12/Lar²¹²⁷ is partially rescued by Scer\GAL4^elav-C155/Lar^D1YLCS.UAS

(Hofmeyer and Treisman, 2009)

Lar^C12/Lar²¹²⁷ is partially rescued by Scer\GAL4^elav-C155/Lar^PPLL.UAS

(Hofmeyer and Treisman, 2009)

Lar^C12/Lar²¹²⁷ is partially rescued by Scer\GAL4^elav-C155/Lar^{PPLL.D1CS.UAS}

(Hofmeyer and Treisman, 2009)

Not rescued by

Lar^C12/Lar²¹²⁷ is not rescued by Scer\GAL4^elav-C155/Lar^ΔFn789.UAS

(Hofmeyer and Treisman, 2009)

Lar^C12/Lar²¹²⁷ is not rescued by Scer\GAL4^elav-C155/Lar^ΔFn2-9.UAS

(Hofmeyer and Treisman, 2009)

Lar^C12/Lar²¹²⁷ is not rescued by Scer\GAL4^elav-C155/Lar^ΔPTP-D2.UAS

(Hofmeyer et al., 2006)

Lar^C12/Lar²¹²⁷ is not rescued by Scer\GAL4^T155/Lar^{ΔC.UAS.Tag:SS(wg),Tag:HA}

(Hofmeyer, 2006)

Comments

Expression of Lar^{Scer\UAS.T:Ivir\HA1}, Lar^{C1638S.Scer\UAS}, Lar^{C1929S.Scer\UAS}, Lar^{CSX2.Scer\UAS}, Lar^{D1YLCS.Scer\UAS}, Lar^{ΔIg123.Scer\UAS}, Lar^{ΔFn123.Scer\UAS}, Lar^{ΔFn456.Scer\UAS}, or Lar^{ΔIg123ΔFn1-6.Scer\UAS.T:wg,T:Ivir\HA1} under the control of Scer\GAL4^elav-C155 in Lar^C12/Lar²¹²⁷ mutant eye discs rescues axon mistargeting. Expression of Lar^{PPLL.Scer\UAS} or Lar^{PPLL.D1CS.Scer\UAS} only partially rescues the axon targeting phenotype. Expression of Lar^{ΔFn789.Scer\UAS} or Lar^{ΔFn2-9.Scer\UAS} fail to rescue the axon targeting phenotype.

Expression of Lar^{Scer\UAS.T:Ivir\HA1} or Lar^{PPLL.Scer\UAS} under the control of Scer\GAL4^elav-C155 in Lar^C12/Lar²¹²⁷ mutant third instar larvae rescues synaptic growth at the neuromuscular junction. Expression of Lar^{C1638S.Scer\UAS}, Lar^{D1YLCS.Scer\UAS}, or Lar^{CSX2.Scer\UAS} only partially rescues this phenotype.

(Hofmeyer and Treisman, 2009)

Expression of Lar^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^elav-C155 rescues R7 targeting in Lar²¹²⁷/Lar^C12 mutants.

Expression of Lar^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^elav-C155 fails to rescue R7 targeting in Lar²¹²⁷/Lar^C12 mutants.

(Hofmeyer et al., 2006)

Expression of Lar^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^T155 rescues the egg elongation phenotype observed in stage 14 oocytes from Lar²¹²⁷/Lar^C12 mutants.

Expression of Lar^{ΔC.Scer\UAS.T:wg,T:Ivir\HA1} under the control of Scer\GAL4^T155 fails to rescue the egg elongation phenotype observed in stage 14 oocytes from Lar²¹²⁷/Lar^C12 mutants and indeed exacerbates the phenotype, with approximately 44% of the oocytes displaying a 'rounded phenotype', compared to approximately 30% in the Lar²¹²⁷/Lar^C12 mutant.

(Hofmeyer, 2006)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Selected as: a mutation that results in photoreceptor projection defects in mosaic animals in which the retina, but no other tissue is homozygous.

(Maurel-Zaffran et al., 2001)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Dlar^C12

(Nern et al., 2008)

LAR^C12

(Clandinin et al., 2001, Maurel-Zaffran et al., 2001)

LAR^c12

(Cameron et al., 2013)

Lar^C12

(Hofmeyer and Treisman, 2009)

Lar^c12

(Astigarraga et al., 2010, Hofmeyer, 2006, Hofmeyer et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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