FlyBase Allele Report: Dmel\Liprin-α[oos]

General Information

Symbol

Dmel\Liprin-α^oos

Species

D. melanogaster

Name

FlyBase ID

FBal0193553

Feature type

allele

Associated gene

Dmel\Liprin-α

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

Nucleotide substitution: C?T.

(Hofmeyer et al., 2006)

Amino acid replacement: Q307term.

(Hofmeyer et al., 2006)

Mutation is within the N-terminal coiled-coil domain.

(Hofmeyer et al., 2006)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:6,726,484..6,726,484 [+]

Nucleotide change:

C6726484T

Reported nucleotide change:

C?T

Amino acid change:

Reported amino acid change:

Q307term

(Hofmeyer et al., 2006)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy

(Astigarraga et al., 2010, Hofmeyer et al., 2006)

abnormal neuroanatomy | somatic clone

(Hofmeyer et al., 2006)

male sterile | recessive

(Astigarraga et al., 2010)

partially lethal - majority die | recessive

(Astigarraga et al., 2010)

Phenotype Manifest In

Detailed Description

Statement

Reference

Liprin-α^oos mutants show mis-targeting of R7 axons from the medulla M6 layer to the M3 layer.

(Douthit et al., 2021)

R7 axons terminate prematurely in the same target layer in the medulla, M3, as R8 axons.

The number of synaptic boutons at the muscle 6/7 synapses in abdominal segments A2 and A3 in Liprin-α^oos animals is ~70% of that from control larvae.

Viability is 19-22% of the expected number.

(Astigarraga et al., 2010)

Liprin-α^oos mutants exhibit aberrant R7 photoreceptor targeting, where approximately 37% of R7 axons project beyond the R8 layer. R1-R6 targeting is as wild-type.

Liprin-α^oos mutants exhibit a normal R7 photoreceptor projection pattern at 24hrs.

There is no obvious difference between the adult medulla of Liprin-α^oos heterozygotes and Liprin-α^oos homozygous mutants.

Embryos lacking both maternal and zygotic Liprin-α^oos do not display a 'bypass' phenotype (resulting from a failure of the ISNb branch of the projection to defasciculate from the ISN branch).

Liprin-α^oos somatic clones exhibit approximately 50% normal R7 targeting.

(Hofmeyer et al., 2006)

Stage 14 oocytes from Liprin-α^oos females are indistinguishable from wild-type.

Stage 14 oocytes from Liprin-α^oos/Df(2L)Exel7027 females are indistinguishable from wild-type.

(Hofmeyer, 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Liprin-α^oos has partially lethal - majority die | recessive phenotype, enhanceable by Liprin-β^Δ51

(Astigarraga et al., 2010)

Liprin-α^oos has abnormal neuroanatomy | somatic clone phenotype, enhanceable by Lar^C12

(Hofmeyer et al., 2006)

Suppressed by

Statement

Reference

Liprin-α^oos has abnormal neuroanatomy phenotype, suppressible | partially by Liprin-γ^H1/Liprin-γ^S1

(Astigarraga et al., 2010)

Liprin-α^oos has partially lethal - majority die | recessive phenotype, suppressible | partially by Liprin-γ^H1/Liprin-γ^S1

(Astigarraga et al., 2010)

Liprin-α^oos has male sterile | recessive phenotype, suppressible by Liprin-γ^H1/Liprin-γ^S1

(Astigarraga et al., 2010)

Liprin-α^oos has abnormal neuroanatomy phenotype, suppressible | partially by trio^GMR.PN

(Astigarraga et al., 2010)

NOT suppressed by

Statement

Reference

Liprin-α^oos has abnormal neuroanatomy phenotype, non-suppressible by Scer\GAL4^elav-C155/Lar^UAS.Tag:HA

(Hofmeyer et al., 2006)

Enhancer of

Statement

Reference

Liprin-α^oos is an enhancer of partially lethal - majority die | recessive phenotype of Liprin-β^Δ51

(Astigarraga et al., 2010)

Liprin-α^oos is an enhancer of abnormal neuroanatomy | somatic clone phenotype of Lar^C12

(Hofmeyer et al., 2006)

Other

Statement

Reference

Liprin-α^oos, Liprin-β^Δ83 has abnormal neuroanatomy phenotype

(Astigarraga et al., 2010)

Liprin-α^oos, Liprin-β^Δ51 has abnormal neuroanatomy phenotype

(Astigarraga et al., 2010)

Liprin-α^oos, Liprin-β^RNAi.UAS, Scer\GAL4^sev.EP has abnormal neuroanatomy phenotype

(Astigarraga et al., 2010)

Phenotype Manifest In

Enhanced by

Statement

Reference

Liprin-α^oos has photoreceptor cell R7 | adult stage phenotype, enhanceable by loaf^JF03040/Scer\GAL4^GMR.long

(Douthit et al., 2021)

Liprin-α^oos has axon terminus | adult stage phenotype, enhanceable by loaf^JF03040/Scer\GAL4^GMR.long

(Douthit et al., 2021)

Liprin-α^oos has outer medulla | adult stage phenotype, enhanceable by loaf^JF03040/Scer\GAL4^GMR.long

(Douthit et al., 2021)

Liprin-α^oos has NMJ bouton | larval stage phenotype, enhanceable by Liprin-β^Δ51

(Astigarraga et al., 2010)

Liprin-α^oos has photoreceptor cell R7 | somatic clone phenotype, enhanceable by Lar^C12

(Hofmeyer et al., 2006)

NOT Enhanced by

Statement

Reference

Liprin-α^oos has NMJ bouton | larval stage phenotype, non-enhanceable by Liprin-γ^H1/Liprin-γ^S1

(Astigarraga et al., 2010)

Suppressed by

Statement

Reference

Liprin-α^oos has photoreceptor cell R7 phenotype, suppressible | partially by trio^GMR.PN

(Astigarraga et al., 2010)

Liprin-α^oos has medulla phenotype, suppressible | partially by trio^GMR.PN

(Astigarraga et al., 2010)

Liprin-α^oos, Liprin-β^Δ51 has NMJ bouton | larval stage phenotype, suppressible | partially by Liprin-γ^H1/Liprin-γ^S1

(Astigarraga et al., 2010)

Liprin-α^oos has photoreceptor cell R7 phenotype, suppressible | partially by Liprin-γ^H1/Liprin-γ^S1

(Astigarraga et al., 2010)

Liprin-α^oos has medulla phenotype, suppressible | partially by Liprin-γ^H1/Liprin-γ^S1

(Astigarraga et al., 2010)

Liprin-α^oos has NMJ bouton | larval stage phenotype, suppressible by Scer\GAL4^elav-C155/trio^UAS.cBa

(Astigarraga et al., 2010)

NOT suppressed by

Statement

Reference

Liprin-α^oos has NMJ bouton | larval stage phenotype, non-suppressible by Liprin-γ^H1/Liprin-γ^S1

(Astigarraga et al., 2010)

Liprin-α^oos has photoreceptor cell R7 phenotype, non-suppressible by Scer\GAL4^elav-C155/Lar^UAS.Tag:HA

(Hofmeyer et al., 2006)

Enhancer of

Statement

Reference

Liprin-α^oos is an enhancer of axon terminus | adult stage phenotype of Scer\GAL4^GMR.long, loaf^JF03040

(Douthit et al., 2021)

Liprin-α^oos is an enhancer of outer medulla | adult stage phenotype of Scer\GAL4^GMR.long, loaf^JF03040

(Douthit et al., 2021)

Liprin-α^oos is an enhancer of photoreceptor cell R7 | adult stage phenotype of Scer\GAL4^GMR.long, loaf^JF03040

(Douthit et al., 2021)

Liprin-α^oos is an enhancer of NMJ bouton | larval stage phenotype of Liprin-β^Δ51

(Astigarraga et al., 2010)

Liprin-α^oos is an enhancer of photoreceptor cell R7 | somatic clone phenotype of Lar^C12

(Hofmeyer et al., 2006)

Other

Statement

Reference

Liprin-α^oos, Liprin-β^Δ83 has photoreceptor cell R7 phenotype

(Astigarraga et al., 2010)

Liprin-α^oos, Liprin-β^Δ83 has medulla phenotype

(Astigarraga et al., 2010)

Liprin-α^oos, Liprin-β^Δ51 has photoreceptor cell R7 phenotype

(Astigarraga et al., 2010)

Liprin-α^oos, Liprin-β^Δ51 has medulla phenotype

(Astigarraga et al., 2010)

Liprin-α^oos, Liprin-β^RNAi.UAS, Scer\GAL4^sev.EP has photoreceptor cell R7 phenotype

(Astigarraga et al., 2010)

Liprin-α^oos, Liprin-β^RNAi.UAS, Scer\GAL4^sev.EP has medulla phenotype

(Astigarraga et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

In Liprin-α^oos, Liprin-β^Δ83 or Liprin-α^oos, Liprin-β^Δ51 double mutants, many R7 axons terminate prematurely in the M3 layer of the medulla. However, a few R7 axons extend far beyond the boundary of the medulla.

Expression of Liprin-β^{dsRNA.Scer\UAS} using Scer\GAL4^sev.EP in a Liprin-α^oos background results in many R7 axons terminating prematurely in the M3 layer of the medulla, with a few R7 axons extending far beyond the boundary of the medulla.

The premature termination of R7 axons in Liprin-α^oos mutants is strongly rescued by trio^GMR.PN.

Liprin-α^oos, Liprin-β^Δ51 double mutants show an additional 25-30% decrease in bouton number compared with either single mutant. The viability of double mutants (4% of the expected number) is reduced compared to either single mutant.

Liprin-α^oos, Liprin-β^Δ51, Liprin-γ^S1/Liprin-γ^H1 triple mutant synapses are larger than Liprin-α^oos, Liprin-β^Δ51 double mutants.

Liprin-α^oos, Liprin-γ^S1/Liprin-γ^H1 double mutants show significantly improved R7 targeting compared with Liprin-α^oos single mutants. Double mutants also show increased viability compared to Liprin-α^oos single mutants, and double mutant males are fertile whereas Liprin-α^oos single mutant males are sterile.

Scer\GAL4^elav-C155-mediated expression of trio^Scer\UAS.cBa restores the number of boutons in Liprin-α^oos animals to control levels.

(Astigarraga et al., 2010)

Overexpression of Lar^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^elav-C155 in a Liprin-α^oos mutant background has little effect on R7 targeting.

Liprin-α^oos Lar^C12 somatic clones exhibit defects in R7 targeting in over 80% of cases. This is a statistically significant difference to wild-type. They have fewer correctly targeted R7 axons than clones homozygous for Lar^C12.

(Hofmeyer et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Liprin-α^oos is rescued by Scer\GAL4^elav-C155/Liprin-α^UAS.Tag:HA

(Hofmeyer et al., 2006)

Not rescued by

Liprin-α^oos is not rescued by Scer\GAL4^elav-C155/Liprin-α^{ΔN.UAS.Tag:HA}

(Hofmeyer et al., 2006)

Comments

Expression of Liprin-α^{Scer\UAS.T:Ivir\HA1} in the neurons, under the control of Scer\GAL4^elav-C155 rescues aberrant R7 photoreceptor targeting in Liprin-α^oos mutants.

Expression of Liprin-α^ΔN.Scer\UAS under the control of Scer\GAL4^elav-C155 does not rescue the Liprin-α^oos mutant phenotype.

(Hofmeyer et al., 2006)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

63808

w^*; Liprin-α^oos P{neoFRT}40A/T(2;3)SM6a-TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Liprin-α^OOS

(Holbrook et al., 2012)

Liprin-α^oos

(Douthit et al., 2021, Astigarraga et al., 2010, Hofmeyer, 2006, Hofmeyer et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (5)

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