FlyBase Allele Report: Dmel\frac[Δ1]

General Information

Symbol

Dmel\frac^Δ1

Species

D. melanogaster

Name

FlyBase ID

FBal0266870

Feature type

allele

Associated gene

Dmel\frac

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

transposable element activity

Nature of the Allele

Allele class

Mutagen

transposable element activity

(Miller et al., 2011)

Progenitor genotype

frac^MB05690

(Miller et al., 2011)

Cytology

Description

Imprecise excision of Mi{ET1}frac^MB05690 results in a 1.2kb deletion, removing part of the first intron and second exon of the frac gene.

(Miller et al., 2011)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | embryonic stage

(Miller et al., 2011)

abnormal neuroanatomy | embryonic stage (with Df(3L)pbl-X1)

(Miller et al., 2011)

fertile

(Miller et al., 2011)

viable

(Miller et al., 2011)

Phenotype Manifest In

larval intersegmental nerve branch ISNb of A1-7 | embryonic stage

(Miller et al., 2011)

larval intersegmental nerve branch ISNb of A1-7 | embryonic stage (with Df(3L)pbl-X1)

(Miller et al., 2011)

larval segmental nerve branch SNa of A1-7 | embryonic stage

(Miller et al., 2011)

larval segmental nerve branch SNa of A1-7 | embryonic stage (with Df(3L)pbl-X1)

(Miller et al., 2011)

Detailed Description

Statement

Reference

frac^Δ1 mutants are viable and display no overt behavioral defects.

frac^Δ1 mutants do not display defects in muscle size, number, or epidermal attachment.

frac^Δ1 mutant embryos exhibit prominent axon guidance defects. Approximately 64% of ISNb axons in frac^Δ1 mutant embryos do not stay tightly bundled and display targeting errors in which individual axons circle back or project to neighbouring nerve bundles. Further classification of these defects reveals that 51% of ISNb nerves display hypo-fasciculated or frayed axons, and 29% include axons that make connections with other nerve branches or other ISNb axons. Approximately 44% of ISNb nerves exhibit ectopic splintering of axons, and 22% display erroneous projections. The penetrance of ISNb guidance defects is not affected by maternal frac levels.

Approximately 49% of hemisegments in frac^Δ1 contain axons that separate inappropriately and project ectopically. The majority of defects are hypo-fasciculation errors in which axons inappropriately branch away from SNa axons.

frac^Δ1/Df(3L)pbl-X1 mutants exhibit the same guidance defects as frac^Δ1 homozygotes.

(Miller et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

frac^Δ1 has abnormal neuroanatomy | embryonic stage phenotype, enhanceable by Mmp2[+]/Mmp2^W307stop

(Miller et al., 2011)

frac^Δ1 has abnormal neuroanatomy | embryonic stage phenotype, enhanceable by LIMK1^EY08757/LIMK1[+]

(Miller et al., 2011)

Suppressed by

Statement

Reference

frac^Δ1 has abnormal neuroanatomy | embryonic stage phenotype, suppressible by Sema1a^k13702/Sema-1a[+]

(Miller et al., 2011)

frac^Δ1 has abnormal neuroanatomy | embryonic stage phenotype, suppressible by Df(2L)N22-5/+

(Miller et al., 2011)

frac^Δ1 has abnormal neuroanatomy | embryonic stage phenotype, suppressible by beat-Ia^C163/beat-Ia[+]

(Miller et al., 2011)

frac^Δ1 has abnormal neuroanatomy | embryonic stage phenotype, suppressible by Scer\GAL4^elav-C155/sax^{Q263D.UAS.Tag:HA}

(Miller et al., 2011)

NOT suppressed by

Statement

Reference

frac^Δ1 has abnormal neuroanatomy | embryonic stage phenotype, non-suppressible by Scer\GAL4^repo.PU/Mmp2^UAS.cPa

(Miller et al., 2011)

Enhancer of

Statement

Reference

frac^Δ1/frac[+] is an enhancer of abnormal neuroanatomy | embryonic stage phenotype of Mmp2^W307stop

(Miller et al., 2011)

frac^Δ1/frac[+] is an enhancer of abnormal neuroanatomy | embryonic stage phenotype of LIMK1^EY08757

(Miller et al., 2011)

Suppressor of

Statement

Reference

frac^Δ1 is a suppressor of abnormal neuroanatomy | embryonic stage phenotype of Mmp2^UAS.cPa, Scer\GAL4^repo.PU

(Miller et al., 2011)

Phenotype Manifest In

Enhanced by

Statement

Reference

frac^Δ1 has larval intersegmental nerve branch ISNb of A1-7 | embryonic stage phenotype, enhanceable by Mmp2[+]/Mmp2^W307stop

(Miller et al., 2011)

frac^Δ1 has larval intersegmental nerve branch ISNb of A1-7 | embryonic stage phenotype, enhanceable by LIMK1^EY08757/LIMK1[+]

(Miller et al., 2011)

Suppressed by

Statement

Reference

frac^Δ1 has larval segmental nerve branch SNa of A1-7 | embryonic stage phenotype, suppressible by Scer\GAL4^elav-C155/sax^{Q263D.UAS.Tag:HA}

(Miller et al., 2011)

frac^Δ1 has larval intersegmental nerve branch ISNb of A1-7 | embryonic stage phenotype, suppressible by Sema1a^k13702/Sema-1a[+]

(Miller et al., 2011)

frac^Δ1 has larval intersegmental nerve branch ISNb of A1-7 | embryonic stage phenotype, suppressible by Df(2L)N22-5/+

(Miller et al., 2011)

frac^Δ1 has larval intersegmental nerve branch ISNb of A1-7 | embryonic stage phenotype, suppressible by beat-Ia^C163/beat-Ia[+]

(Miller et al., 2011)

frac^Δ1 has larval intersegmental nerve branch ISNb of A1-7 | embryonic stage phenotype, suppressible by Scer\GAL4^elav-C155/sax^{Q263D.UAS.Tag:HA}

(Miller et al., 2011)

NOT suppressed by

Statement

Reference

frac^Δ1 has larval intersegmental nerve branch ISNb of A1-7 | embryonic stage phenotype, non-suppressible by Scer\GAL4^repo.PU/Mmp2^UAS.cPa

(Miller et al., 2011)

frac^Δ1 has larval segmental nerve branch SNa of A1-7 | embryonic stage phenotype, non-suppressible by Scer\GAL4^repo.PU/Mmp2^UAS.cPa

(Miller et al., 2011)

Enhancer of

Statement

Reference

frac^Δ1/frac[+] is an enhancer of larval intersegmental nerve branch ISNb of A1-7 | embryonic stage phenotype of Mmp2^W307stop

(Miller et al., 2011)

frac^Δ1/frac[+] is an enhancer of larval intersegmental nerve branch ISNb of A1-7 | embryonic stage phenotype of LIMK1^EY08757

(Miller et al., 2011)

Suppressor of

Statement

Reference

frac^Δ1 is a suppressor of larval intersegmental nerve branch ISNb of A1-7 | embryonic stage phenotype of Mmp2^UAS.cPa, Scer\GAL4^repo.PU

(Miller et al., 2011)

frac^Δ1 is a suppressor of larval segmental nerve branch SNa of A1-7 | embryonic stage phenotype of Mmp2^UAS.cPa, Scer\GAL4^repo.PU

(Miller et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Mmp2^W307stop/+ ; frac^Δ1/+ double heterozygotes exhibit elevated levels of ISNb and SNa guidance defects. The incidence of ISNb mis-projection rises from 1% and 2% in frac^Δ1 and Mmp2^W307stop single heterozygotes, respectively, to 18% in the double heterozygote.

A heterozygous Sema-1a^k13702 background dominantly suppresses the ISNb pathfinding phenotypes from 64% in frac^Δ1 homozygotes to 18% in double mutants.

A Df(2L)N22-5 background dominantly suppresses the ISNb pathfinding phenotypes from 64% in frac^Δ1 homozygotes to 18% in double mutants.

A heterozygous beat-Ia^C163 background dominantly suppresses the ISNb pathfinding phenotypes from 64% in frac^Δ1 homozygotes to 18% in double mutants.

A homozygous frac^Δ1 mutant background suppresses the excess fasciculation seen in embryos ove-rexpressing Mmp2^Scer\UAS.cPa under the control of Scer\GAL4^repo.PU. Furthermore, these embryos display diminished motor axon bundling similar to that observed in frac^Δ1 homozygotes.

Pan-neuronal expression of sax^{Q263D.Scer\UAS.T:Ivir\HA1} reduces the incidence of defasciculation from 64% to 30% in frac^Δ1 mutants.

The ISNb of LIMK1^EY08757; frac^Δ1 double heterozygous mutant embryos is defasciculated and has ectopic projections. These double mutants display an increase in mis-projections relative to either heterozygote alone.

(Miller et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

frac^Δ1

(Miller et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (1)

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