FlyBase Allele Report: Dmel\eyg[M3-12]

eyg^22-2 in combination with the allele eyg^M3-12 causes a range of phenotypes in pharate adults: 20% are headless, 35% are eyeless, and 45% have eyes smaller than 400 ommatidia. Furthermore, 100% have a grooved thorax phenotype. The following transgenes cause a dominant enhancement of the grooved thorax and reduced eye phenotype of eyg^22-2/eyg^M3-12 mutants, when expressed under the control of Scer\GAL4^eyg-22-2 : eyg^{ΔPD.Scer\UAS}, eyg^{ΔRED.Scer\UAS}, eyg^{ΔL.ΔRED.Scer\UAS}, eyg^C.Scer\UAS, and eyg^{ΔPAI.ΔL.Scer\UAS}. eyg^{Scer\UAS.T:Hsim\VP16} and eyg^mPD.Scer\UAS expression enhances the grooved thorax phenotype. Expression of eyg^{mPD.mHD.Scer\UAS} or eyg^{Scer\UAS.T:Hsim\VP16} causes a headless phenotype with 100% penetrance.

(Yao and Sun, 2005)

eyg¹/eyg^M3-12 flies have no eyes. eyg^M3-12 homozygous flies have no head.

(Chao et al., 2004)

eyg^XE119/eyg^M3-12 transheterozygotes show a phenotype in which flies have tiny eyes or completely lack eyes altogether. These mutants also have a severe groove on the thorax.

(Dominguez et al., 2004)

Pharate adults have a headless phenotype - all structures derived from the eye-antennal discs are missing. The prominent remaining structure is the labellum. The fish-trap bristles are also present. In eyg^M3-12 larvae, no eye-antennal discs can be found. eyg¹/eyg^M3-12 animals exhibit a small eye phenotype.

(Jang et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

eyg^M3-12/eyg[+] is an enhancer of visible phenotype of Caf1-105^NIG.12892R, Scer\GAL4^ey.PH

(Yu et al., 2013)

Other

Statement

Reference

N^ICN.UAS, Scer\GAL4^ey.PH, eyg^M3-12 has partially lethal - majority die phenotype

(Chao et al., 2004)

Phenotype Manifest In

Enhanced by

Statement

Reference

eyg^22-2/eyg^M3-12 has eye | pharate adult stage phenotype, enhanceable by Scer\GAL4^eyg-22-2/Hsap\PAX6^5a.UAS

(Yao and Sun, 2005)

eyg^22-2/eyg^M3-12 has thorax | pharate adult stage phenotype, enhanceable by Scer\GAL4^eyg-22-2/Hsap\PAX6^5a.UAS

(Yao and Sun, 2005)

Suppressed by

Statement

Reference

eyg^22-2/eyg^M3-12 has eye | pharate adult stage phenotype, suppressible by ey::eyg^GGEGGG.UAS/Scer\GAL4^eyg-22-2

(Yao and Sun, 2005)

eyg^22-2/eyg^M3-12 has thorax | pharate adult stage phenotype, suppressible by ey::eyg^GGEGGG.UAS/Scer\GAL4^eyg-22-2

(Yao and Sun, 2005)

eyg^22-2/eyg^M3-12 has head | pharate adult stage phenotype, suppressible by ey::eyg^GGEGGG.UAS/Scer\GAL4^eyg-22-2

(Yao and Sun, 2005)

eyg¹/eyg^M3-12 has eye phenotype, suppressible | partially by H[+]/H^E31

(Chao et al., 2004)

eyg¹/eyg^M3-12 has eye phenotype, suppressible | partially by N^ICN.UAS/Scer\GAL4^dpp.blk1

(Chao et al., 2004)

eyg¹/eyg^M3-12 has eye phenotype, suppressible | partially by Scer\GAL4^dpp.blk1/Su(H)^UAS.cNa

(Chao et al., 2004)

eyg^M3-12 has head phenotype, suppressible by H[+]/H^E31

(Chao et al., 2004)

eyg^M3-12 has head phenotype, suppressible by N^ICN.UAS/Scer\GAL4^ey.PH

(Chao et al., 2004)

eyg¹/eyg^M3-12 has eye phenotype, suppressible by Scer\GAL4^dpp.blk1/upd1^UAS.cZa

(Chao et al., 2004)

eyg¹/eyg^M3-12 has eye phenotype, suppressible | partially by ey^UAS.cHa/Scer\GAL4^ey.PH

(Jang et al., 2003)

NOT suppressed by

Statement

Reference

eyg^22-2/eyg^M3-12 has eye | pharate adult stage phenotype, non-suppressible by Scer\GAL4^eyg-22-2/ey::eyg^GGEGEG.UAS

(Yao and Sun, 2005)

eyg^22-2/eyg^M3-12 has eye | pharate adult stage phenotype, non-suppressible by Scer\GAL4^eyg-22-2/ey::eyg^GGGGEG.UAS

(Yao and Sun, 2005)

eyg^22-2/eyg^M3-12 has head | pharate adult stage phenotype, non-suppressible by Scer\GAL4^eyg-22-2/ey::eyg^GGEGEG.UAS

(Yao and Sun, 2005)

eyg^22-2/eyg^M3-12 has head | pharate adult stage phenotype, non-suppressible by Scer\GAL4^eyg-22-2/ey::eyg^GGGGEG.UAS

(Yao and Sun, 2005)

eyg^22-2/eyg^M3-12 has thorax | pharate adult stage phenotype, non-suppressible by Scer\GAL4^eyg-22-2/ey::eyg^GGEGEG.UAS

(Yao and Sun, 2005)

eyg^22-2/eyg^M3-12 has thorax | pharate adult stage phenotype, non-suppressible by Scer\GAL4^eyg-22-2/ey::eyg^GGGGEG.UAS

(Yao and Sun, 2005)

eyg¹/eyg^M3-12 has eye phenotype, non-suppressible by Scer\GAL4^ey.PH/BacA\p35^UAS.cHa

(Jang et al., 2003)

eyg¹/eyg^M3-12 has eye phenotype, non-suppressible by Scer\GAL4^dpp.blk1/BacA\p35^UAS.cHa

(Jang et al., 2003)

Enhancer of

Statement

Reference

eyg^M3-12/eyg[+] is an enhancer of eye phenotype of Caf1-105^NIG.12892R, Scer\GAL4^ey.PH

(Yu et al., 2013)

eyg^M3-12/eyg[+] is an enhancer of eye phenotype of Df(1)os¹

(Chao et al., 2004)

eyg^M3-12 is an enhancer of eye phenotype of pb^Rev3.HSPB

(Benassayag et al., 2003)

NOT Suppressor of

Statement

Reference

eyg^M3-12/Scer\GAL4^dpp.blk1 is a non-suppressor of eye | ectopic phenotype of Scer\GAL4^dpp.blk1, ey^UAS.cHa

(Jang et al., 2003)

Other

Statement

Reference

Delta^RevF10, Ser[+]/Ser^RX82, eyg^M3-12/eyg[+] has eye phenotype

(Chao et al., 2004)

Delta^RevF10/Dl[+], Ser[+]/Ser^RX82, eyg^M3-12 has eye phenotype

(Chao et al., 2004)

N^ICN.UAS, Scer\GAL4^ey.PH, eyg^M3-12 has eye disc phenotype

(Chao et al., 2004)

N^ICN.UAS, Scer\GAL4^ey.PH, eyg^M3-12 has antennal disc | increased number phenotype

(Chao et al., 2004)

N^ICN.UAS, Scer\GAL4^ey.PH, eyg^M3-12 has eye disc | ectopic phenotype

(Chao et al., 2004)

Delta^RevF10/Dl[+], Ser^RX82, eyg^M3-12/eyg[+] has eye phenotype

(Chao et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

One copy of eyg^M3-12 enhances the small eye phenotype seen when Caf1-105^NIG.12892R is expressed under the control of Scer\GAL4^ey.PH.

(Yu et al., 2013)

Expression of ey::eyg^{GGEGGG.Scer\UAS} rescues the eyg^22-2/eyg^M3-12 or ey::eyg^{GGEGEG.Scer\UAS}, fails to rescue the mutant phenotype.

(Yao and Sun, 2005)

Dl^RevF10, Ser^RX82 transheterozygotes have normal sized eyes, but eyg^M3-12; Dl^RevF10, Ser^RX82 triple heterozygous flies have small eyes. H^E31/+ partially restores eye development in eyg¹/eyg^M3-12 flies, as does N^ICN.Scer\UAS; Scer\GAL4^dpp.blk1 or Su(H)^Scer\UAS.cNa; Scer\GAL4^dpp.blk1. Head development in eyg^M3-12 homozygous flies is partially rescued by H^E31/+: the resulting flies appear to have a complete head apart from lacking eyes. Head development in these flies is also partially rescued by N^ICN.Scer\UAS; Scer\GAL4^ey.PH : although only 6% of these animals survive to the pharate stage, those that do have heads but usually lack eyes. The eye-antennal discs of eyg^M3-12/eyg^M3-12; N^ICN.Scer\UAS; Scer\GAL4^ey.PH larvae have a highly reduced eye field with no photoreceptor differentiation, but an extra eye field with photoreceptor duplication is present dorsal to the endogenous eye field in about a third of cases. In contrast the antennal field of these discs is always present and is sometimes duplicated or triplicated. upd1^Scer\UAS.cZa; Scer\GAL4^dpp.blk1 completely restores eye development in eyg¹/eyg^M3-12 flies.

(Chao et al., 2004)

The addition of ey^Scer\UAS.cHa driven by Scer\GAL4^ey.PH can partially rescue the eyg^M3-12/eyg¹ mutant eye phenotype.

(Jang et al., 2003)

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\PAX6^5a.Scer\UAS, under the control of Scer\GAL4^eyg-22-2, enhances the eyg^22-2/eyg^M3-12 mutant eye and thorax phenotypes.

(Yao and Sun, 2005)

Complementation and Rescue Data

Rescued by

eyg^22-2/eyg^M3-12 is rescued by Scer\GAL4^eyg-22-2/eyg^ΔC.UAS

(Yao and Sun, 2005)

eyg^22-2/eyg^M3-12 is rescued by Scer\GAL4^eyg-22-2/eyg^{554.UAS.Tag:NLS(SV40-largeT),Tag:FLAG}

(Yao and Sun, 2005)

eyg^22-2/eyg^M3-12 is rescued by Scer\GAL4^eyg-22-2/eyg^ΔPAI.UAS

(Yao and Sun, 2005)

eyg^22-2/eyg^M3-12 is rescued by eyg^325.UAS/Scer\GAL4^eyg-22-2

(Yao and Sun, 2005)

eyg^22-2/eyg^M3-12 is rescued by Scer\GAL4^eyg-22-2/eyg^en.UAS

(Yao and Sun, 2005)

eyg^22-2/eyg^M3-12 is rescued by Scer\GAL4^eyg-22-2/eyg^{670.UAS.Tag:FLAG}

(Yao and Sun, 2005)

eyg^22-2/eyg^M3-12 is rescued by eyg^438.UAS/Scer\GAL4^eyg-22-2

(Yao and Sun, 2005)

eyg^22-2/eyg^M3-12 is rescued by Scer\GAL4^eyg-22-2/eyg^{554.UAS.Tag:FLAG}

(Yao and Sun, 2005)

eyg^22-2/eyg^M3-12 is rescued by Scer\GAL4^eyg-22-2/eyg^{643.UAS.Tag:FLAG}

(Yao and Sun, 2005)

eyg¹/eyg^M3-12 is rescued by Scer\GAL4^upd1-E132/eyg^UAS.lune

(Jang et al., 2003)

eyg¹/eyg^M3-12 is rescued by Scer\GAL4^ey.PH/eyg^UAS.lune

(Jang et al., 2003)

eyg¹/eyg^M3-12 is rescued by eyg^hs.lune

(Jang et al., 2003)

Partially rescued by

eyg^M3-12 is partially rescued by Scer\GAL4^CD/eyg^UAS.lune

(Wang et al., 2008)

eyg^M3-12 is partially rescued by Scer\GAL4^EX/eyg^UAS.lune

(Wang et al., 2008)

eyg^M3-12 is partially rescued by Scer\GAL4^F1/eyg^UAS.lune

(Wang et al., 2008)

eyg^M3-12 is partially rescued by Scer\GAL4^XS/eyg^UAS.lune

(Wang et al., 2008)

eyg^M3-12 is partially rescued by Scer\GAL4^CD/Scer\GAL80^ts.αTub84B/eyg^UAS.lune

(Wang et al., 2008)

eyg^22-2/eyg^M3-12 is partially rescued by Scer\GAL4^eyg-22-2/eyg^mPD.UAS

(Yao and Sun, 2005)

eyg^22-2/eyg^M3-12 is partially rescued by eyg^mHD.UAS/Scer\GAL4^eyg-22-2

(Yao and Sun, 2005)

eyg^22-2/eyg^M3-12 is partially rescued by Scer\GAL4^eyg-22-2/eyg^ΔHD.UAS

(Yao and Sun, 2005)

eyg¹/eyg^M3-12 is partially rescued by Scer\GAL4^eyg.E2-EX/eyg^UAS.lune

(Chao et al., 2004)

eyg^M3-12 is partially rescued by Scer\GAL4^eyg.E2-EX/eyg^UAS.lune

(Chao et al., 2004)

Not rescued by

eyg^22-2/eyg^M3-12 is not rescued by Scer\GAL4^eyg-22-2/eyg^ΔPD.UAS

(Yao and Sun, 2005)

eyg^22-2/eyg^M3-12 is not rescued by eyg^ΔRED.UAS/Scer\GAL4^eyg-22-2

(Yao and Sun, 2005)

eyg^22-2/eyg^M3-12 is not rescued by Scer\GAL4^eyg-22-2/eyg^{ΔL.ΔRED.UAS}

(Yao and Sun, 2005)

eyg^22-2/eyg^M3-12 is not rescued by Scer\GAL4^eyg-22-2/eyg^C.UAS

(Yao and Sun, 2005)

eyg^22-2/eyg^M3-12 is not rescued by Scer\GAL4^eyg-22-2/eyg^{ΔPAI.ΔL.UAS}

(Yao and Sun, 2005)

eyg^22-2/eyg^M3-12 is not rescued by eyg^VP16.UAS/Scer\GAL4^eyg-22-2

(Yao and Sun, 2005)

eyg^22-2/eyg^M3-12 is not rescued by Scer\GAL4^eyg-22-2/eyg^mPD.mHD.UAS

(Yao and Sun, 2005)

Comments

Expression of eyg^{Scer\UAS.lune} under the control of Scer\GAL4^CD rescues the loss of the eye-antennal disc and headless phenotype of the eyg^M3-12 mutant. In some cases, duplication of the larval eye-antennal disc and adult antenna occurs. The notum of these animals remains grooved.

Expression of eyg^{Scer\UAS.lune} under the control of Scer\GAL4^EX, Scer\GAL4^F1, or Scer\GAL4^XS partially rescues the eyg^M3-12 mutant. In all cases a small head with antennae but without eyes develops. The rescue efficiency of Scer\GAL4^XS (76%) and Scer\GAL4^EX (60%) are both much higher than Scer\GAL4^F1 (7%).

Expression of eyg^{Scer\UAS.lune} under the combined control of Scer\GAL4^EX and Scer\GAL4^XS partially rescues the eyg^M3-12 mutant to a slightly higher percentage (85%) than either driver alone. In this case the same level of rescue is observed, with flies having a small head with antennae but no eyes.

Expression of eyg^{Scer\UAS.lune} under the transient control of Scer\GAL4^CD during stage L2 (expression restricted by Scer\GAL80^ts.αTub84B throughout other stages of development) significantly rescues the eyg^M3-12 eye mutant phenotype. Earlier and later expression are not sufficient to rescue the eye development.

(Wang et al., 2008)

Expression of eyg^mPD.Scer\UAS, under the control of Scer\GAL4^eyg-22-2, partially rescues the eye phenotype of eyg^22-2/eyg^M3-12 mutants, but has a dominant-negative effect on the thorax phenotype. Expression of eyg^{ΔHD.Scer\UAS} in a eyg^22-2/eyg^M3-12 background leads to partial rescue of the mutant eye and mutant thorax phenotypes. Expression of the eyg^mHD.Scer\UAS transgene in a eyg^22-2/eyg^M3-12 background leads to the full rescue of eyes in 21% of mutants. The mutant thorax is fully rescued, although there are still many extra macrochaetes at lateral regions. Coexpression of the transgenes eyg^mHD.Scer\UAS and eyg^mPD.Scer\UAS in a eyg^22-2/eyg^M3-12 background fully rescues the mutant eye and thorax phenotypes.

(Yao and Sun, 2005)

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Mutant

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Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

eyg^M3-12

(Yu et al., 2013, Gutierrez-Aviño et al., 2009, Wang et al., 2008)

eyg^M

(Benassayag et al., 2003)

Name Synonyms

Secondary FlyBase IDs

References (8)

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