FlyBase Allele Report: Dmel\eyg[22-2]

General Information

Symbol

Dmel\eyg^22-2

Species

D. melanogaster

Name

FlyBase ID

FBal0150505

Feature type

allele

Associated gene

Dmel\eyg

Associated Insertion(s)

P{GawB}eyg^22-2

Carried in Construct

Also Known As

eyg-Gal4

Key Links

Allele class

hypomorphic allele - genetic evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Yao and Sun, 2005)

Mutagen

P-element activity

(Jang et al., 2003)

Progenitor genotype

P{GawB}EM458

(Jang et al., 2003)

Associated Insertion(s)

P{GawB}eyg^22-2

Cytology

Description

The P{GawB} has transposed 8bp downstream and is accompanied by an 159bp deletion.

(Jang et al., 2003)

Allele components

Component

Use(s)

Inserted element

P{GawB}

enhancer trap

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

eye | pharate adult stage (with eyg^M3-12)

(Yao and Sun, 2005)

eye | pharate adult stage (with eyg^M3-12), with Scer\GAL4^eyg-22-2, eyg^C.UAS

(Yao and Sun, 2005)

eye | pharate adult stage (with eyg^M3-12), with Scer\GAL4^eyg-22-2, eyg^{ΔL.ΔRED.UAS}

(Yao and Sun, 2005)

eye | pharate adult stage (with eyg^M3-12), with Scer\GAL4^eyg-22-2, eyg^{ΔPAI.ΔL.UAS}

(Yao and Sun, 2005)

eye | pharate adult stage (with eyg^M3-12), with Scer\GAL4^eyg-22-2, eyg^ΔPD.UAS

(Yao and Sun, 2005)

eye | pharate adult stage (with eyg^M3-12), with Scer\GAL4^eyg-22-2, eyg^ΔRED.UAS

(Yao and Sun, 2005)

head (with eyg^M3-12), with Scer\GAL4^eyg-22-2, eyg^mPD.mHD.UAS

(Yao and Sun, 2005)

head (with eyg^M3-12), with Scer\GAL4^eyg-22-2, eyg^VP16.UAS

(Yao and Sun, 2005)

head | pharate adult stage (with eyg^M3-12)

(Yao and Sun, 2005)

head | pharate adult stage (with eyg^M3-12), with Scer\GAL4^eyg-22-2, eyg^mPD.mHD.UAS

(Yao and Sun, 2005)

head | pharate adult stage (with eyg^M3-12), with Scer\GAL4^eyg-22-2, eyg^VP16.UAS

(Yao and Sun, 2005)

thorax | pharate adult stage (with eyg^M3-12)

(Yao and Sun, 2005)

thorax | pharate adult stage (with eyg^M3-12), with Scer\GAL4^eyg-22-2, eyg^C.UAS

(Yao and Sun, 2005)

thorax | pharate adult stage (with eyg^M3-12), with Scer\GAL4^eyg-22-2, eyg^mPD.UAS

(Yao and Sun, 2005)

thorax | pharate adult stage (with eyg^M3-12), with Scer\GAL4^eyg-22-2, eyg^VP16.UAS

(Yao and Sun, 2005)

thorax | pharate adult stage (with eyg^M3-12), with Scer\GAL4^eyg-22-2, eyg^{ΔL.ΔRED.UAS}

(Yao and Sun, 2005)

thorax | pharate adult stage (with eyg^M3-12), with Scer\GAL4^eyg-22-2, eyg^{ΔPAI.ΔL.UAS}

(Yao and Sun, 2005)

thorax | pharate adult stage (with eyg^M3-12), with Scer\GAL4^eyg-22-2, eyg^ΔPD.UAS

(Yao and Sun, 2005)

thorax | pharate adult stage (with eyg^M3-12), with Scer\GAL4^eyg-22-2, eyg^ΔRED.UAS

(Yao and Sun, 2005)

Detailed Description

Statement

Reference

eyg^22-2 in combination with the allele eyg^M3-12 causes a range of phenotypes in pharate adults: 20% are headless, 35% are eyeless, and 45% have eyes smaller than 400 ommatidia. Furthermore, 100% have a grooved thorax phenotype. The following transgenes cause a dominant enhancement of the grooved thorax and reduced eye phenotype of eyg^22-2/eyg^M3-12 mutants, when expressed under the control of Scer\GAL4^eyg-22-2 : eyg^{ΔPD.Scer\UAS}, eyg^{ΔRED.Scer\UAS}, eyg^{ΔL.ΔRED.Scer\UAS}, eyg^C.Scer\UAS, and eyg^{ΔPAI.ΔL.Scer\UAS}. eyg^{Scer\UAS.T:Hsim\VP16} and eyg^mPD.Scer\UAS expression enhances the grooved thorax phenotype. Expression of eyg^{mPD.mHD.Scer\UAS} or eyg^{Scer\UAS.T:Hsim\VP16} causes a headless phenotype with 100% penetrance.

(Yao and Sun, 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

eyg^22-2/eyg^M3-12 has eye | pharate adult stage phenotype, enhanceable by Scer\GAL4^eyg-22-2/Hsap\PAX6^5a.UAS

(Yao and Sun, 2005)

eyg^22-2/eyg^M3-12 has thorax | pharate adult stage phenotype, enhanceable by Scer\GAL4^eyg-22-2/Hsap\PAX6^5a.UAS

(Yao and Sun, 2005)

Suppressed by

Statement

Reference

eyg^22-2/eyg^M3-12 has eye | pharate adult stage phenotype, suppressible by ey::eyg^GGEGGG.UAS/Scer\GAL4^eyg-22-2

(Yao and Sun, 2005)

eyg^22-2/eyg^M3-12 has thorax | pharate adult stage phenotype, suppressible by ey::eyg^GGEGGG.UAS/Scer\GAL4^eyg-22-2

(Yao and Sun, 2005)

eyg^22-2/eyg^M3-12 has head | pharate adult stage phenotype, suppressible by ey::eyg^GGEGGG.UAS/Scer\GAL4^eyg-22-2

(Yao and Sun, 2005)

NOT suppressed by

Statement

Reference

eyg^22-2/eyg^M3-12 has eye | pharate adult stage phenotype, non-suppressible by Scer\GAL4^eyg-22-2/ey::eyg^GGEGEG.UAS

(Yao and Sun, 2005)

eyg^22-2/eyg^M3-12 has eye | pharate adult stage phenotype, non-suppressible by Scer\GAL4^eyg-22-2/ey::eyg^GGGGEG.UAS

(Yao and Sun, 2005)

eyg^22-2/eyg^M3-12 has head | pharate adult stage phenotype, non-suppressible by Scer\GAL4^eyg-22-2/ey::eyg^GGEGEG.UAS

(Yao and Sun, 2005)

eyg^22-2/eyg^M3-12 has head | pharate adult stage phenotype, non-suppressible by Scer\GAL4^eyg-22-2/ey::eyg^GGGGEG.UAS

(Yao and Sun, 2005)

eyg^22-2/eyg^M3-12 has thorax | pharate adult stage phenotype, non-suppressible by Scer\GAL4^eyg-22-2/ey::eyg^GGEGEG.UAS

(Yao and Sun, 2005)

eyg^22-2/eyg^M3-12 has thorax | pharate adult stage phenotype, non-suppressible by Scer\GAL4^eyg-22-2/ey::eyg^GGGGEG.UAS

(Yao and Sun, 2005)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of ey::eyg^{GGEGGG.Scer\UAS} rescues the eyg^22-2/eyg^M3-12 mutant phenotype. However, expression of either ey::eyg^{GGGGEG.Scer\UAS} or ey::eyg^{GGEGEG.Scer\UAS}, fails to rescue the mutant phenotype.

(Yao and Sun, 2005)

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\PAX6^5a.Scer\UAS, under the control of Scer\GAL4^eyg-22-2, enhances the eyg^22-2/eyg^M3-12 mutant eye and thorax phenotypes.

(Yao and Sun, 2005)

Complementation and Rescue Data

Fails to complement

eyg¹

(Jang et al., 2003)

Rescued by

eyg^22-2/eyg^M3-12 is rescued by Scer\GAL4^eyg-22-2/eyg^ΔC.UAS

(Yao and Sun, 2005)

eyg^22-2/eyg^M3-12 is rescued by Scer\GAL4^eyg-22-2/eyg^{554.UAS.Tag:NLS(SV40-largeT),Tag:FLAG}

(Yao and Sun, 2005)

eyg^22-2/eyg^M3-12 is rescued by Scer\GAL4^eyg-22-2/eyg^ΔPAI.UAS

(Yao and Sun, 2005)

eyg^22-2/eyg^M3-12 is rescued by eyg^325.UAS/Scer\GAL4^eyg-22-2

(Yao and Sun, 2005)

eyg^22-2/eyg^M3-12 is rescued by Scer\GAL4^eyg-22-2/eyg^en.UAS

(Yao and Sun, 2005)

eyg^22-2/eyg^M3-12 is rescued by Scer\GAL4^eyg-22-2/eyg^{670.UAS.Tag:FLAG}

(Yao and Sun, 2005)

eyg^22-2/eyg^M3-12 is rescued by eyg^438.UAS/Scer\GAL4^eyg-22-2

(Yao and Sun, 2005)

eyg^22-2/eyg^M3-12 is rescued by Scer\GAL4^eyg-22-2/eyg^{554.UAS.Tag:FLAG}

(Yao and Sun, 2005)

eyg^22-2/eyg^M3-12 is rescued by Scer\GAL4^eyg-22-2/eyg^{643.UAS.Tag:FLAG}

(Yao and Sun, 2005)

Partially rescued by

eyg^22-2/eyg^M3-12 is partially rescued by Scer\GAL4^eyg-22-2/eyg^mPD.UAS

(Yao and Sun, 2005)

eyg^22-2/eyg^M3-12 is partially rescued by eyg^mHD.UAS/Scer\GAL4^eyg-22-2

(Yao and Sun, 2005)

eyg^22-2/eyg^M3-12 is partially rescued by Scer\GAL4^eyg-22-2/eyg^ΔHD.UAS

(Yao and Sun, 2005)

Not rescued by

eyg^22-2/eyg^M3-12 is not rescued by Scer\GAL4^eyg-22-2/eyg^ΔPD.UAS

(Yao and Sun, 2005)

eyg^22-2/eyg^M3-12 is not rescued by eyg^ΔRED.UAS/Scer\GAL4^eyg-22-2

(Yao and Sun, 2005)

eyg^22-2/eyg^M3-12 is not rescued by Scer\GAL4^eyg-22-2/eyg^{ΔL.ΔRED.UAS}

(Yao and Sun, 2005)

eyg^22-2/eyg^M3-12 is not rescued by Scer\GAL4^eyg-22-2/eyg^C.UAS

(Yao and Sun, 2005)

eyg^22-2/eyg^M3-12 is not rescued by Scer\GAL4^eyg-22-2/eyg^{ΔPAI.ΔL.UAS}

(Yao and Sun, 2005)

eyg^22-2/eyg^M3-12 is not rescued by eyg^VP16.UAS/Scer\GAL4^eyg-22-2

(Yao and Sun, 2005)

eyg^22-2/eyg^M3-12 is not rescued by Scer\GAL4^eyg-22-2/eyg^mPD.mHD.UAS

(Yao and Sun, 2005)

Comments

Expression of eyg^mPD.Scer\UAS, under the control of Scer\GAL4^eyg-22-2, partially rescues the eye phenotype of eyg^22-2/eyg^M3-12 mutants, but has a dominant-negative effect on the thorax phenotype. Expression of eyg^{ΔHD.Scer\UAS} in a eyg^22-2/eyg^M3-12 background leads to partial rescue of the mutant eye and mutant thorax phenotypes. Expression of the eyg^mHD.Scer\UAS transgene in a eyg^22-2/eyg^M3-12 background leads to the full rescue of eyes in 21% of mutants. The mutant thorax is fully rescued, although there are still many extra macrochaetes at lateral regions. Coexpression of the transgenes eyg^mHD.Scer\UAS and eyg^mPD.Scer\UAS in a eyg^22-2/eyg^M3-12 background fully rescues the mutant eye and thorax phenotypes.

(Yao and Sun, 2005)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

58458

w^*; P{UAS-GFP.S65T}Myo31DF^T2; P{GawB}eyg^22-2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

eyg^22-2

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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