The DNA binding residues of the PD and HD domains have been disrupted (amino acid replacements S92A, S94A, S95A, N97A, S281A and N282A).
Unlike overexpression of wild-type eyg, expression of eygmPD.mHD.Scer\UAS, under the control of Scer\GAL4dpp.blk1, does not cause extra ventral eye formation. Expression of eygmPD.mHD.Scer\UAS in a eyg22-2/eygM3-12 background causes a headless phenotype with 100% penetrance.
Scer\GAL4eyg-22-2/eygmPD.mHD.UAS fails to rescue eyg22-2/eygM3-12
Expression of eygmPD.mHD.Scer\UAS, under the control of Scer\GAL4eyg-22-2, in a eyg22-2/eygM3-12 background causes a headless phenotype with 100% penetrance.