Amino acid replacement: R?H. The amino acid replacement is at position 5 with respect to the beginning of the homeobox.
Nucleotide substitution: G118A. Amino acid replacement: R202H. In exon 4.
Point mutation (CGC to CAC), changes the Arg at the N terminus (5th residue) of the homeodomain to His.
G6711911A
G118A
R202H | pb-PA; R197H | pb-PB; R197H | pb-PC; R192H | pb-PD
R202H
2 copies of pbRev3.HSPB leads to flies with a slightly reduced eye. Four copies leads to a complete deletion of the eye.
Flies carrying pbRev3.HSPB show eye loss.
Flies carrying one copy of pbRev3.HSPB are wild type. Flies carrying two copies of pbRev3.HSPB have a single ectopic fine bristle on antennal segment 3. Heat shock causes marked reductions in the eye in flies carrying two copies of pbRev3.HSPB.
Flies appear wild type in the absence of heat shock. One copy of the transgene causes no visible phenotype. Increasing the copies to four causes reduction in eye tissue until flies have no eyes and antenna take on a more rectangular form carrying a single fine bristle.
pbRev3.HSPB has visible phenotype, enhanceable by ey[+]/eyD1Da
pbRev3.HSPB has visible phenotype, enhanceable by ey[+]/eyJD
pbRev3.HSPB has eye phenotype, enhanceable by eyJD
pbRev3.HSPB has eye phenotype, enhanceable by eygM3-12
pbRev3.HSPB has eye phenotype, enhanceable by Df(4)BA
pbRev3.HSPB has eye phenotype, enhanceable by ey2
pbRev3.HSPB has eye phenotype, enhanceable by eyD1Da
pbRev3.HSPB has eye phenotype, enhanceable by eyEH
pbRev3.HSPB has eye phenotype, enhanceable by eyl1
pbRev3.HSPB has eye phenotype, enhanceable by ey[+]/eyD1Da
pbRev3.HSPB has eye phenotype, enhanceable by ey[+]/eyJD
pbRev3.HSPB has eye phenotype, non-enhanceable by eya2
pbRev3.HSPB has eye phenotype, non-enhanceable by eyacli-IID
pbRev3.HSPB has eye phenotype, non-enhanceable by eygc1
pbRev3.HSPB has eye phenotype, non-enhanceable by so1
pbRev3.HSPB has eye phenotype, non-enhanceable by soL31
eyJD, pbRev3.HSPB has antenna phenotype
eyJD, pbRev3.HSPB has maxillary palp phenotype
The combination of 2 copies of eyJD with pbRev3.HSPB often leads to flies with complete eye loss and the fusion of the antenna and maxillary palp. In some cases, the maxillary palp, remains adjoined to the antennal appendage. However differentiation od the proboscis is not affected. The addition of eyEH, or eyD1Da to flies with 2 copies of pbRev3.HSPB, leads to a strong enhancement of the eye loss phenotype. The addition of ey2, or Df(4)BA to flies with 2 copies of pbRev3.HSPB, leads to an enhancement of the eye loss phenotype. The addition of eyl1, or eygM3-12 to flies with 2 copies of pbRev3.HSPB, leads to a weak enhancement of the eye loss phenotype. The addition of so1, soL31, eya2, eyacli-IID or eygc1 to flies with 2 copies of pbRev3.HSPB, does not enhance the eye loss phenotype.
eyJD and eyD1Da dominantly enhance the eye loss phenotype of pbRev3.HSPB flies.
Loss of function allele in the mouthparts and aristae and gain of function in the proximal antenna and eyes.