FlyBase Allele Report: Dmel\pb[hs.PB]

General Information

Symbol

Dmel\pb^hs.PB

Species

D. melanogaster

Name

heat shock construct of Boube

FlyBase ID

FBal0039734

Feature type

allele

Associated gene

Dmel\pb

Associated Insertion(s)

Carried in Construct

P{HSPB}

Key Links

Transgenic product class

wild_type

(Cribbs et al., 1995)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Cribbs et al., 1995)

Mutagen

in vitro construct

(Boube et al., 1998, Benassayag et al., 1997, Benassayag et al., 1997, Boube et al., 1997, Boube et al., 1995, Cribbs et al., 1995)

Progenitor genotype

Carried in construct

P{HSPB}

Cytology

Description

Hsp70 promoter governing expression of pb minigene. Includes all the pb coding region apart from the 15bp alternatively spliced microexon.

(Cribbs et al., 1995)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible

(Boube et al., 1998)

visible | dominant

(Benassayag et al., 1997, Benassayag et al., 1997, Boube et al., 1997, Cribbs et al., 1995)

visible | heat sensitive

(Boube et al., 1995)

wild-type

(Benassayag et al., 1997, Benassayag et al., 1997, Cribbs et al., 1995)

Phenotype Manifest In

antenna

(Boube et al., 1998, Benassayag et al., 1997, Benassayag et al., 1997, Boube et al., 1997, Boube et al., 1995)

arista

(Boube et al., 1997, Kuzin et al., 1997, Cribbs et al., 1995)

dorsal row of wing sensilla

(Cribbs et al., 1995)

eye | anterior

(Cribbs et al., 1995)

eye | ventral

(Benassayag et al., 1997)

fourth segment of antenna

(Boube et al., 1997)

head

(Cribbs et al., 1995)

head | posterior

(Benassayag et al., 1997)

interommatidial bristle

(Benassayag et al., 1997)

macrochaeta | ectopic

(Boube et al., 1997)

maxillary palp | ectopic

(Cribbs et al., 1995)

microchaeta & wing

(Cribbs et al., 1995)

ommatidium

(Benassayag et al., 1997)

prothoracic leg

(Benassayag et al., 1997)

prothoracic leg & sex comb

(Cribbs et al., 1995)

prothoracic leg | distal

(Boube et al., 2000)

second segment of antenna

(Cribbs et al., 1995)

sex comb

(Cribbs et al., 1995)

sex comb | ectopic

(Boube et al., 2000, Cribbs et al., 1995)

third segment of antenna

(Boube et al., 1997, Cribbs et al., 1995)

ventral row of wing sensilla

(Cribbs et al., 1995)

vertical bristle 2

(Cribbs et al., 1995)

wing

(Benassayag et al., 1997)

wing sensillum

(Boube et al., 1998)

wing vein L3

(Boube et al., 1997)

wing vein L3 & macrochaeta

(Boube et al., 1997)

Detailed Description

Statement

Reference

The induction of pb^hs.PB occasionally exhibit an ectopic distal sex comb on the T1 leg.

(Boube et al., 2000)

Flies expressing pb^hs.PB show a progressive transformation of antennae to maxillary palps as the copy number of P{HSPB} elements is increased. The transformation does not affect the most proximal antennal tissues, and acts differentially along the proximo-distal axis, from proximal toward distal. Flies carrying two copies of pb^hs.PB show loss of many distal, but not proximal, wing margin mechanosensory bristles. This phenotype is enhanced by H^DMu4.

(Boube et al., 1998)

Flies carrying pb^hs.PB show a fully penetrant, dose-sensitive dominant transformation of antennae towards maxillary palps in the absence of heat shock.

(Benassayag et al., 1997)

Uninduced expression causes antenna to maxillary palps transformation. Subtle dose-sensitive defects are also observed in the eyes, posterior head, prothoracic legs and wings. Two copies of the insertion cause reduction or loss of the ventral aspect of the adult eye, a frequent change in ommatidial geometry, from hexagonal to tetragonal, in remaining ommatidia and duplication of some interommatidial bristles. Hemizygous males have normal eyes.

(Benassayag et al., 1997)

Flies expressing a single copy of pb^hs.PB at a basal level (in the absence of heat shock) show a partial transformation of antennae to maxillary palps. Approximately 10% of females and 2% of males have an ectopic bristle on the distal end of wing vein L3. Flies carrying two copies of pb^hs.PB show a nearly complete antenna to maxillary transformation.

(Boube et al., 1997)

Heat induced expression does not affect tarsal leg structures but causes some thickening of the arista.

(Kuzin et al., 1997)

Dose sensitive dominant transformation of antenna towards maxillary palps with 100% penetrance, this phenotype can be induced in the absence of heat shock.

(Boube et al., 1995)

Uninduced expression of pb^hs.PB causes a novel, fully penetrant dominant transformation of antennal segment 3 and the aristae towards maxillary palps. Antennal segment 2 is perturbed but not explicitly transformed. Transformation of the arista can be augmented by altering the culture temperature from 22^oC to 29^oC. In general flies exhibit reduced viability and mobility. Dose sensitive effects were noticed for prothoracic legs (ectopic and perturbed sex combs), head capsule (malformed outer vertical bristles, reduction of the anterior aspect of the eye) and wings (loss or alteration of the anterior wing margin bristles, and formation of microchaetae at the distal extremity of wing vein L3). Overexpression by heat induction does not generate marked increase in adult cuticular phenotypes.

(Cribbs et al., 1995)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

pb^hs.PB has visible phenotype, enhanceable by RasGAP1^sxt-BJ61

(Boube et al., 1997)

Suppressed by

Statement

Reference

RasGAP1^sxt-BJ61, pb^hs.PB/pb[+] has visible phenotype, suppressible by Ras85D^e1B/Ras85D[+]

(Boube et al., 1997)

RasGAP1^sxt-BJ61, pb^hs.PB has visible phenotype, suppressible by Ras85D^e1B/Ras85D[+]

(Boube et al., 1997)

Enhancer of

Statement

Reference

pb^hs.PB is an enhancer of visible phenotype of RasGAP1^sxt-BJ61

(Boube et al., 1997)

Other

Statement

Reference

pb^hs.PB, tara^L4 has visible | dominant phenotype

(Calgaro et al., 2002)

H^E31, Ras85D^e1F, pb^hs.PB has visible | dominant phenotype

(Boube et al., 1998)

Ras85D^e1B, RasGAP1^sxt-BJ61, pb^hs.PB/pb[+] has visible phenotype

(Boube et al., 1997)

Ras85D^e1B, pb^hs.PB has visible phenotype

(Boube et al., 1997)

Ras85D^e1B, pb^hs.PB has visible | dominant phenotype

(Boube et al., 1997)

Df(3R)by10, pb^hs.PB has visible | dominant phenotype

(Boube et al., 1997)

Df(3R)by416, pb^hs.PB has visible | dominant phenotype

(Boube et al., 1997)

Df(3R)GB104, pb^hs.PB has visible | dominant phenotype

(Boube et al., 1997)

pb^hs.PB, ss^a40aw has visible phenotype

(Kuzin et al., 1997)

Phenotype Manifest In

Enhanced by

Statement

Reference

pb^hs.PB has prothoracic leg | distal phenotype, enhanceable by skd^pap-53

(Boube et al., 2000)

pb^hs.PB has sex comb | ectopic phenotype, enhanceable by skd^pap-53

(Boube et al., 2000)

pb^hs.PB has wing sensillum phenotype, enhanceable by H^DMu4

(Boube et al., 1998)

pb^hs.PB has antenna phenotype, enhanceable by RasGAP1^sxt-BJ61

(Boube et al., 1997)

Suppressed by

Statement

Reference

pb^hs.PB, skd^pap-53 has prothoracic leg | distal phenotype, suppressible by Scr⁴

(Boube et al., 2000)

pb^hs.PB, skd^pap-53 has sex comb | ectopic phenotype, suppressible by Scr⁴

(Boube et al., 2000)

pb^hs.PB has prothoracic leg | distal phenotype, suppressible by Scr⁴

(Boube et al., 2000)

pb^hs.PB has sex comb | ectopic phenotype, suppressible by Scr⁴

(Boube et al., 2000)

RasGAP1^sxt-BJ61, pb^hs.PB/pb[+] has antenna phenotype, suppressible by Ras85D^e1B/Ras85D[+]

(Boube et al., 1997)

RasGAP1^sxt-BJ61, pb^hs.PB/pb[+] has wing vein phenotype, suppressible by Ras85D^e1B/Ras85D[+]

(Boube et al., 1997)

RasGAP1^sxt-BJ61, pb^hs.PB has antenna phenotype, suppressible by Ras85D^e1B/Ras85D[+]

(Boube et al., 1997)

RasGAP1^sxt-BJ61, pb^hs.PB has wing vein phenotype, suppressible by Ras85D^e1B/Ras85D[+]

(Boube et al., 1997)

Enhancer of

Statement

Reference

pb^hs.PB is an enhancer of wing vein phenotype of RasGAP1^sxt-BJ61

(Boube et al., 1997)

Other

Statement

Reference

pb^hs.PB, tara^L4 has wing vein L5 phenotype

(Calgaro et al., 2002)

pb^hs.PB, tara^L4 has wing vein L4 phenotype

(Calgaro et al., 2002)

pb^hs.PB, skd^pap-53 has sex comb | ectopic phenotype

(Boube et al., 2000)

pb^hs.PB, skd^pap-53 has prothoracic leg | distal phenotype

(Boube et al., 2000)

H^E31, Ras85D^e1F, pb^hs.PB has wing vein L5 phenotype

(Boube et al., 1998)

H^E31, Ras85D^e1F, pb^hs.PB has wing vein L4 phenotype

(Boube et al., 1998)

H^E31, Ras85D^e1F, pb^hs.PB has campaniform sensillum of dorsal radius phenotype

(Boube et al., 1998)

Ras85D^e1B, RasGAP1^sxt-BJ61, pb^hs.PB/pb[+] has wing vein phenotype

(Boube et al., 1997)

Ras85D^e1B, RasGAP1^sxt-BJ61, pb^hs.PB/pb[+] has antenna phenotype

(Boube et al., 1997)

Ras85D^e1B, pb^hs.PB has wing vein L5 phenotype

(Boube et al., 1997)

Ras85D^e1B, pb^hs.PB has wing vein L3 phenotype

(Boube et al., 1997)

Ras85D^e1B, pb^hs.PB has wing vein L4 phenotype

(Boube et al., 1997)

Df(3R)by10, pb^hs.PB has antenna phenotype

(Boube et al., 1997)

Df(3R)by416, pb^hs.PB has antenna phenotype

(Boube et al., 1997)

Df(3R)GB104, pb^hs.PB has antenna phenotype

(Boube et al., 1997)

pb^hs.PB, ss^a40aw has tarsal segment phenotype

(Kuzin et al., 1997)

pb^hs.PB, ss^a40aw has antennal segment phenotype

(Kuzin et al., 1997)

Antp^B, pb^hs.PB has antenna phenotype

(Cribbs et al., 1995)

Antp^B, pb^hs.PB has maxillary palp | ectopic phenotype

(Cribbs et al., 1995)

Antp^R, pb^hs.PB has antenna phenotype

(Cribbs et al., 1995)

Antp^R, pb^hs.PB has maxillary palp | ectopic phenotype

(Cribbs et al., 1995)

Antp^RM, pb^hs.PB has antenna phenotype

(Cribbs et al., 1995)

Antp^RM, pb^hs.PB has maxillary palp | ectopic phenotype

(Cribbs et al., 1995)

Antp^Wu, pb^hs.PB has antenna phenotype

(Cribbs et al., 1995)

Antp^Wu, pb^hs.PB has maxillary palp | ectopic phenotype

(Cribbs et al., 1995)

Antp^CB, pb^hs.PB has antenna phenotype

(Cribbs et al., 1995)

Antp^CB, pb^hs.PB has maxillary palp | ectopic phenotype

(Cribbs et al., 1995)

Antp^Ctx, pb^hs.PB has antenna phenotype

(Cribbs et al., 1995)

Antp^Ctx, pb^hs.PB has maxillary palp | ectopic phenotype

(Cribbs et al., 1995)

Antp^W, pb^hs.PB has antenna phenotype

(Cribbs et al., 1995)

Antp^W, pb^hs.PB has maxillary palp | ectopic phenotype

(Cribbs et al., 1995)

Antp^DC, pb^hs.PB has antenna phenotype

(Cribbs et al., 1995)

Antp^DC, pb^hs.PB has maxillary palp | ectopic phenotype

(Cribbs et al., 1995)

Antp^PW, pb^hs.PB has antenna phenotype

(Cribbs et al., 1995)

Antp^PW, pb^hs.PB has maxillary palp | ectopic phenotype

(Cribbs et al., 1995)

Additional Comments

Genetic Interactions

Statement

Reference

60% of flies carrying pb^hs.PB that are also heterozygous for tara^L4 show partial truncation of the distal part of wing vein L4. This phenotype is not seen in either pb^hs.PB single mutant or tara^L4/+ single mutant flies. Deletions of wing vein L5 are also seen in the double mutant flies.

(Calgaro et al., 2002)

The addition of Scr⁴ suppresses and the addition of skd^pap-53 enhances the ectopic distal sex comb phenotype. The addition of Scr⁴ to pb^hs.PB, skd^pap-53 flies also suppresses the distal sex comb phenotype.

(Boube et al., 2000)

pb^hs.PB H^E31/Ras85D^e1F triple heterozygotes have deletions of the distal portions of wing veins L4 and L5, up to and including the posterior crossvein. The spacing and position of the campaniform sensilla along the proximo-distal axis of wing vein L3 is altered.

(Boube et al., 1998)

Shows a dose-sensitive interaction with Df(2L)net-PMF, Df(2L)30A-C, Df(2L)TW50, Df(2R)en30, Df(2R)PC4, Df(3L)GN24, Df(3L)AC1, Df(3R)by10, Df(3R)by416, Df(3R)GB104, Df(3R)P115 and Df(3R)L127. Does not interact with Df(3R)by62. Df(3R)by10, Df(3R)by416 and Df(3R)GB104 reduce the antennal to maxillary transformation caused by pb^hs.PB. pb^hs.PB Ras85D^e1B double heterozygotes have a partially penetrant truncation of wing veins L4 and L5, and the frequency of ectopic bristle formation on the distal end of wing vein L3 (seen in pb^hs.PB heterozygotes) is increased to 90% for females and 50% for males. pb^hs.PB Df(3L)AC1 double heterozygotes show a very weak modification of the pb^hs.PB wing phenotype. Gap1^sxt-BJ61 enhances the antenna to maxillary transformation in pb^hs.PB/+ Gap1^sxt-BJ61/Gap1^sxt-BJ61 flies. This enhancement is reduced if the flies also carry one copy of Ras85D^e1B. Enhances the ectopic wing vein phenotype seen in Gap1^sxt-BJ61 homozygotes. This enhancement is reduced if the flies also carry one copy of Ras85D^e1B.

(Boube et al., 1997)

Induction of expression in ss^a40aw flies causes shortening of the distal parts of the tarsus, mostly in the antennae, legs are less affected.

(Kuzin et al., 1997)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

pb^hs.PB rescues pb⁴

(Benassayag et al., 1997)

pb^hs.PB rescues pb⁴

(Benassayag et al., 1997)

pb^hs.PB rescues pb^unspecified

(Cribbs et al., 1995)

pb^hs.PB rescues pb⁴

(Cribbs et al., 1995)

Partially rescues

pb^hs.PB partially rescues pb⁵

(Benassayag et al., 1997)

pb^hs.PB partially rescues pb⁵

(Cribbs et al., 1995)

Comments

Rescues the phenotype of recessive pb mutations.

(Cribbs et al., 1995)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Elevated temperatures supplement the level of expression of this allele. Attempts to generate a fly containing three copies have been unsuccessful.

(Cribbs et al., 1995)

Flies carrying pb^hs.PB have been used in a genetic screen for dominant modifiers of pb function.

(Boube et al., 1997)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

pb^hs.PB

Name Synonyms

heat shock construct of Boube

Secondary FlyBase IDs

References (11)

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