FlyBase Allele Report: Dmel\Cam[7]

General Information

Symbol

Dmel\Cam⁷

Species

D. melanogaster

Name

FlyBase ID

FBal0083596

Feature type

allele

Associated gene

Dmel\Cam

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Harrisingh et al., 2007)

Mutagen

ethyl methanesulfonate

(Nelson et al., 1997)

Progenitor genotype

Cytology

Description

Amino acid replacement: V91G.

(Wang et al., 2000)

Amino acid replacement: V91G. Alters a residue in the N-terminal helix of binding site 3.

(Nelson et al., 1997)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:12,269,289..12,269,289 [+]

Nucleotide change:

T12269289G

Amino acid change:

Reported amino acid change:

V91G

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Nelson et al., 1997)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal behavior (with Camⁿ³³⁹), with Cam^UAS.cWa, Scer\GAL4^how-24B

(Wang et al., 2003)

abnormal behavior | larval stage

(Wang et al., 2000)

abnormal mating (with Camⁿ³³⁹), with Cam^UAS.cWa, Scer\GAL4^how-24B

(Wang et al., 2003)

decreased rate of movement | larval stage (with Camⁿ³³⁹)

(Wang et al., 2003)

lethal

(Dean et al., 2015, Wang et al., 2000)

lethal (with Camⁿ³³⁹)

(Wang et al., 2002)

lethal - all die before end of larval stage, with Cam^B34Q.UAS, Scer\GAL4^how-24B

(Wang et al., 2002)

lethal - all die before end of larval stage (with Camⁿ³³⁹), with Cam^B34Q.UAS, Scer\GAL4^how-24B

(Wang et al., 2002)

lethal - all die before end of P-stage (with Camⁿ³³⁹)

(Wang et al., 2003)

lethal - all die before end of pupal stage | recessive

(Nelson et al., 1997)

some die during larval stage, with Cam^B34Q.UAS, Scer\GAL4^how-24B

(Wang et al., 2002)

some die during larval stage (with Camⁿ³³⁹), with Cam^B34Q.UAS, Scer\GAL4^how-24B

(Wang et al., 2002)

some die during pharate adult stage (with Camⁿ³³⁹)

(Wang et al., 2003)

some die during pupal stage | recessive

(Nelson et al., 1997)

Phenotype Manifest In

adult head (with Camⁿ³³⁹)

(Wang et al., 2003)

adult head | P-stage

(Wang et al., 2000, Nelson et al., 1997)

adult head | P-stage (with Camⁿ³³⁹)

(Wang et al., 2002)

longitudinal muscle cell (with Camⁿ³³⁹)

(Wang et al., 2003)

pupa

(Nelson et al., 1997)

pupa (with Camⁿ³³⁹)

(Wang et al., 2003, Wang et al., 2002)

pupal cuticle

(Wang et al., 2000)

pupal cuticle (with Camⁿ³³⁹)

(Wang et al., 2003)

wing vein | ectopic

(Nelson et al., 1997)

Detailed Description

Statement

Reference

Cam⁷/+ flies have normal wing structure.

(Dean et al., 2015)

Cam⁷ heterozygotes exhibit little effect on the average period of free-running.

(Harrisingh et al., 2007)

Cam⁷/Camⁿ³³⁹ larvae are morphologically normal and show normal forward locomotion with no spontaneous backward movement. However, they are sluggish with a body wall contraction (BWC) rate about one-third that of controls. Cam⁷/Camⁿ³³⁹ wandering third instar larvae become progressively incapable of relaxing at the end of each body-wall contraction and show increasing stiffness during locomotion. Third instar body wall muscles that have been treated with ether (to artificially relax them) all show longitudinal muscles that have a "bunched" appearance, with structural disorganisation and misaligned myofibrils. Cam⁷/Camⁿ³³⁹ animals form pupal cases with deep indentations at the larval segment boundaries. On average the mutant larvae decrease their body length by 50% during pupariation, compared to a decrease of one-third in wild-type larvae. Only the long axis is abnormally compressed in the mutant animals. Cam⁷/Camⁿ³³⁹ pupae never eclose, but most develop into pharate adults with head defects. Three types of heads are seen; normal heads with no obvious defects, malformed heads that are partially everted and "inside-out" heads where head eversion has completely failed and head development proceeds in a noneverted head sac buried in the thorax. There is a correlation between the pupal length:width ratio and the degree of head abnormality; the more severe head defects are associated with shorter pupal cases. Cam⁷/Camⁿ³³⁹ animals rescued to adulthood by expression of Cam^Scer\UAS.cWa under the control of Scer\GAL4^how-24B are behaviourally abnormal; they fail to climb after being gently knocked to the bottom of a vial, and they show poor flight and a reduced ability to right themselves after a brief vortexing. The initial stages of male courtship are normal, but the rescued males cannot bend their abdomens sufficiently to achieve penetration.

(Wang et al., 2003)

Cam⁷/Camⁿ³³⁹ pupal cases are shorter than wild type and have deeply indented rings at the larval segment boundaries. Many animals have head eversion defects and none eclose. Expression of Cam^{B34Q.Scer\UAS} under the control of Scer\GAL4^how-24B in Cam⁷/+ animals (which are normally phenotypically wild type) results in less than half the animals pupariating and some pupae have body wrinkles similar to that seen in Cam⁷ hemizygotes. Expression of both Cam^{B12Q.Scer\UAS} and Cam^{B34Q.Scer\UAS} under the control of Scer\GAL4^how-24B in Cam⁷/Camⁿ³³⁹ animals results in 100% larval lethality.

(Wang et al., 2002)

Mutant larvae are sluggish and form highly indented pupal cases as a result of excessive longitudinal contraction during pupariation. Many pupae have problems with head eversion and formation and no adults eclose.

(Wang et al., 2000)

Hemizygotes survive to pupate but never emerge from the pupal cases. The pupal cases are smaller than normal with distinctive 'Michelin-man' phenotype of deeply indented rings corresponding to junctions of the larval body segments. Most individuals the develop within the pupal case are headless or have partial head structures due to a failure of head eversion. Inverted head structures, including fully formed eyes, can be dissected out of the thoraces. Some individuals have fully everted heads. Cam⁷/Cam^8t mutants also die in the pupal case but few develop far enough to show failed head eversion. Cam^hs.PN restores good viability, about 50%. Transheterozygous combinations with other Cam mutations produces an incompletely penetrant ectopic wing vein phenotype.

(Nelson et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Cam⁷/Camⁿ³³⁹ has lethal phenotype, suppressible | partially by Ca-α1D^X7/Ca-alpha1D[+]

(Wang et al., 2003)

Cam⁷/Camⁿ³³⁹ has lethal phenotype, suppressible by RyR¹⁶/Rya-r44F[+]

(Wang et al., 2003)

NOT suppressed by

Statement

Reference

Cam⁷/Camⁿ³³⁹ has lethal phenotype, non-suppressible by Ca-α1D^AR66/Ca-alpha1D[+]

(Wang et al., 2003)

Cam⁷/Camⁿ³³⁹ has lethal phenotype, non-suppressible by Df(2R)H3E1/+

(Wang et al., 2003)

Cam⁷/Camⁿ³³⁹ has lethal phenotype, non-suppressible by cn[+]/cn¹

(Wang et al., 2003)

Enhancer of

Statement

Reference

Cam⁷/Cam[+] is an enhancer of abnormal circadian rhythm phenotype of Mmmm\PV^UAS.Tag:MYC, Scer\GAL4^P2.4.Pdf

(Harrisingh et al., 2007)

NOT Suppressor of

Statement

Reference

Cam⁷/Cam[+] is a non-suppressor of visible phenotype of IP3K2^wy-2

(Dean et al., 2015)

Other

Statement

Reference

Cam⁷/Camⁿ³³⁹, RyR¹⁶ has abnormal locomotor behavior phenotype

(Wang et al., 2003)

Cam⁷/Camⁿ³³⁹, RyR¹⁶/Rya-r44F[+] has abnormal locomotor behavior phenotype

(Wang et al., 2003)

Cam⁷, l(2)C43^C43 has partially lethal phenotype

(Nelson et al., 1997)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Cam⁷/Camⁿ³³⁹ has adult head phenotype, non-enhanceable by cn[+]/cn¹

(Wang et al., 2003)

Cam⁷/Camⁿ³³⁹ has pupa phenotype, non-enhanceable by cn[+]/cn¹

(Wang et al., 2003)

Suppressed by

Statement

Reference

Cam⁷/Camⁿ³³⁹ has adult head phenotype, suppressible | partially by Df(2R)H3E1/+

(Wang et al., 2003)

Cam⁷/Camⁿ³³⁹ has pupa phenotype, suppressible by RyR¹⁶/Rya-r44F[+]

(Wang et al., 2003)

Cam⁷/Camⁿ³³⁹ has pupa phenotype, suppressible | partially by Ca-α1D^AR66/Ca-alpha1D[+]

(Wang et al., 2003)

Cam⁷/Camⁿ³³⁹ has pupa phenotype, suppressible | partially by Ca-α1D^X7/Ca-alpha1D[+]

(Wang et al., 2003)

Cam⁷/Camⁿ³³⁹ has pupa phenotype, suppressible | partially by Df(2R)H3E1/+

(Wang et al., 2003)

Cam⁷/Camⁿ³³⁹ has pupal cuticle phenotype, suppressible by RyR¹⁶/Rya-r44F[+]

(Wang et al., 2003)

Cam⁷/Camⁿ³³⁹ has adult head phenotype, suppressible by RyR¹⁶/Rya-r44F[+]

(Wang et al., 2003)

NOT suppressed by

Statement

Reference

Cam⁷/Camⁿ³³⁹ has adult head phenotype, non-suppressible by cn[+]/cn¹

(Wang et al., 2003)

Cam⁷/Camⁿ³³⁹ has pupa phenotype, non-suppressible by cn[+]/cn¹

(Wang et al., 2003)

NOT Suppressor of

Statement

Reference

Cam⁷/Cam[+] is a non-suppressor of wing phenotype of IP3K2^wy-2

(Dean et al., 2015)

Other

Statement

Reference

Ca-α1D^X7, Cam⁷/Camⁿ³³⁹ has wing phenotype

(Wang et al., 2003)

Cam⁷/Camⁿ³³⁹, RyR¹⁶ has wing phenotype

(Wang et al., 2003)

Ca-α1D^X7/Ca-alpha1D[+], Cam⁷/Camⁿ³³⁹ has wing phenotype

(Wang et al., 2003)

Cam⁷/Camⁿ³³⁹, RyR¹⁶/Rya-r44F[+] has wing phenotype

(Wang et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

Cam⁷/+ does not modify wing phenotypes in IP3K2^wy-2/IP3K2^wy-2 flies.

(Dean et al., 2015)

Rya-r44F¹⁶/+ rescues the pupal defects and lethality of Cam⁷/Camⁿ³³⁹ animals; the pupae are smooth and indentation free in the anterior and only have mild ridges in the posterior, their length:width ratios are actually higher than wild type, more than 40% of the pupae eclose and those that do not eclose do not show head defects. Some of the eclosed animals do not show wing expansion and they all perform poorly in a climb test. Df(2R)H3E1/+ has a slight suppressing effect on the Cam⁷/Camⁿ³³⁹ phenotype; pupae show some increase in the pupal length:width ratio, but very few pupal cases have decreased indentations, and fewer head defects are seen in pupae, but they fail to eclose. Ca-α1D^X7/+ has a slight suppressing effect on the Cam⁷/Camⁿ³³⁹ phenotype; there is a small but significant increase in the pupal length:width ratio, but no obvious effect on pupal case indentations, and a small fraction of the animals eclose as weak, uncoordinated adults with no wing expansion. Ca-α1D^AR66/+ has a slight suppressing effect on the Cam⁷/Camⁿ³³⁹ phenotype; there is an increase in the pupal length:width ratio, but none of the pupae eclose.

(Wang et al., 2003)

In combination with l(2)C43^C43 mutants exhibit markedly reduced viability.

(Nelson et al., 1997)

Xenogenetic Interactions

Statement

Reference

A heterozygous Cam⁷ background in flies expressing Mmmm\PV^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^P2.4.Pdf can result in lengthening of the free-running period, compared to controls, after 3-5 weeks in constant darkness.

(Harrisingh et al., 2007)

Complementation and Rescue Data

Partially complements

Cam^3c1

(Nelson et al., 1997)

Cam^4c1

(Nelson et al., 1997)

Cam⁵

(Nelson et al., 1997)

Cam^6c4

(Nelson et al., 1997)

Fails to complement

Camⁿ³³⁹

(Nelson et al., 1997)

Cam^8t

(Nelson et al., 1997)

Rescued by

Cam⁷/Camⁿ³³⁹ is rescued by Scer\GAL4^how-24B/Cam^UAS.cWa

(Wang et al., 2002)

Partially rescued by

Cam⁷/Camⁿ³³⁹ is partially rescued by Scer\GAL4^how-24B/Cam^UAS.cWa

(Wang et al., 2003)

Cam⁷/Camⁿ³³⁹ is partially rescued by Scer\GAL4^how-24B/Cam^B12Q.UAS

(Wang et al., 2002)

Cam⁷ is partially rescued by Cam^hs.PN

(Nelson et al., 1997)

Not rescued by

Cam⁷/Camⁿ³³⁹ is not rescued by Cam^UAS.cWa/Scer\GAL4^elav.PLu

(Wang et al., 2003)

Cam⁷/Camⁿ³³⁹ is not rescued by Scer\GAL4^sca.PU/Cam^UAS.cWa

(Wang et al., 2003)

Cam⁷/Camⁿ³³⁹ is not rescued by Cam^B1234Q.UAS/Scer\GAL4^how-24B

(Wang et al., 2002)

Cam⁷/Camⁿ³³⁹ is not rescued by Cam^B34Q.UAS/Scer\GAL4^how-24B

(Wang et al., 2002)

Comments

The lethality and pupariation defects seen in Cam⁷/Camⁿ³³⁹ animals are rescued by expression of Cam^Scer\UAS.cWa under the control of Scer\GAL4^how-24B; the pupal cases are morphologically wild type and have more normal length:width ratios, almost all of the rescued pupae eclose and no aberrant pharate head structures are seen. The lethality and pupariation defects seen in Cam⁷/Camⁿ³³⁹ animals are not rescued by expression of Cam^Scer\UAS.cWa under the control of Scer\GAL4^elav.PLu or Scer\GAL4^sca.PU. The sluggish larval locomotion of Cam⁷/Camⁿ³³⁹ animals is incompletely rescued by expression of Cam^Scer\UAS.cWa under the control of Scer\GAL4^how-24B or Scer\GAL4^elav.PLu.

(Wang et al., 2003)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

8140

y¹ w^*; J¹ Cam⁷/CyO, y⁺

Notes on Origin

Discoverer

Comments

Allelic series of the relative strength of the lethal mutations: Cam^8t > Cam⁷ > Cam^6c4 > Cam⁵ > Cam^4c1 > Cam^3c1.

(Nelson et al., 1997)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Cam⁷

(Dean et al., 2015, Harrisingh et al., 2007)

Cam^V91G

V91G

(Wang et al., 2002, Wang et al., 2000)

Name Synonyms

Secondary FlyBase IDs

FBal0104882

References (7)

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