FB2024_03 , released June 25, 2024
Allele: Dmel\CamB12Q.UAS
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General Information
Symbol
Dmel\CamB12Q.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0141862
Feature type
allele
Associated gene
Dmel\Cam
Associated Insertion(s)
Carried in Construct
P{UAS-Cam.B12Q}
Key Links
Transgenic product class
missense_variant
(Wang et al., 2002)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Wang et al., 2002)
Mutagen
in vitro construct
(Wang et al., 2002)
Progenitor genotype
Carried in construct
P{UAS-Cam.B12Q}
Cytology
Description

UASt regulatory sequences drive expression of a mutated form of Cam in which the two N-terminal Ca[2+] binding sites have been inactivated by E to Q changes.

(Wang et al., 2002)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Cam
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of CamB12Q.Scer\UAS under the control of Scer\GAL4how-24B in Cam7/+ animals (which are normally phenotypically wild type) has no effect on pupal case structure or survival. Expression of both CamB12Q.Scer\UAS and CamB34Q.Scer\UAS under the control of Scer\GAL4how-24B in Cam7/Camn339 animals results in 100% larval lethality.

      (Wang et al., 2002)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      Scer\GAL4how-24B/CamB12Q.UAS partially rescues Cam7/Camn339

      (Wang et al., 2002)
      Fails to rescue

      CamB12Q.UAS/Scer\GAL4elav.PLu fails to rescue Camn339

      (Wang et al., 2003)
      Comments

      (with CamB34Q.Scer\UAS) Fails to rescue: Camn339 { Scer\GAL4elav.PLu } The spontaneous backward movement seen in homozygous Camn339 larvae is not rescued by expression of CamB12Q.Scer\UAS under the control of Scer\GAL4elav.PLu. The spontaneous backward movement seen in homozygous Camn339 larvae is not rescued by co-expression of CamB12Q.Scer\UAS and CamB34Q.Scer\UAS under the control of Scer\GAL4elav.PLu.

      (Wang et al., 2003)

      Expression of CamB12Q.Scer\UAS under the control of Scer\GAL4how-24B rescues the overcontraction of the larval body seen during pupariation in Cam7/Camn339 animals and produces smooth pupal cases, but the pupal cases are longer and narrower than normal and the anterior larval segments do not retract fully during pupariation. Most of the pupae fail to develop recognisable adult structures and only 9.4% of the pupae eclose.

      (Wang et al., 2002)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      CamB12Q.Scer\UAS
      CamB12Q.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (2)