FB2024_03 , released June 25, 2024
Allele: Dmel\CamUAS.cWa
Open Close
General Information
Symbol
Dmel\CamUAS.cWa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Wang
FlyBase ID
FBal0141860
Feature type
allele
Associated gene
Dmel\Cam
Associated Insertion(s)
Carried in Construct
P{UAS-Cam.W}
Also Known As
UAS-CaM
Key Links
Transgenic product class
wild_type
(Wang et al., 2002)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Wang et al., 2002)
Mutagen
in vitro construct
(Wang et al., 2002)
Progenitor genotype
Carried in construct
P{UAS-Cam.W}
Cytology
Description

UASt regulatory sequences drive expression of wild type Cam.

(Wang et al., 2002)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Cam
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Cam7/Camn339 animals rescued to adulthood by expression of CamScer\UAS.cWa under the control of Scer\GAL4how-24B are behaviourally abnormal; they fail to climb after being gently knocked to the bottom of a vial, and they show poor flight and a reduced ability to right themselves after a brief vortexing. The initial stages of male courtship are normal, but the rescued males cannot bend their abdomens sufficiently to achieve penetration.

      (Wang et al., 2003)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of CamScer\UAS.cWa and NosScer\UAS.cMa under the control of Scer\GAL4ey-OK107 does not significantly affect axon regrowth in the mushroom body gamma-lobe.

      (Rabinovich et al., 2016)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Scer\GAL4how-24B/CamUAS.cWa rescues Cam7/Camn339

      (Wang et al., 2002)
      Partially rescues

      Scer\GAL4how-24B/CamUAS.cWa partially rescues Cam7/Camn339

      (Wang et al., 2003)

      CamUAS.cWa/Scer\GAL4elav.PLu partially rescues Camn339

      (Wang et al., 2003)
      Fails to rescue

      CamUAS.cWa/Scer\GAL4elav.PLu fails to rescue Cam7/Camn339

      (Wang et al., 2003)

      Scer\GAL4sca.PU/CamUAS.cWa fails to rescue Cam7/Camn339

      (Wang et al., 2003)

      Scer\GAL4how-24B/CamUAS.cWa fails to rescue Camn339

      (Wang et al., 2003)

      Scer\GAL4sca.PU/CamUAS.cWa fails to rescue Camn339

      (Wang et al., 2003)
      Comments

      The lethality and pupariation defects seen in Cam7/Camn339 animals are rescued by expression of CamScer\UAS.cWa under the control of Scer\GAL4how-24B; the pupal cases are morphologically wild type and have more normal length:width ratios, almost all of the rescued pupae eclose and no aberrant pharate head structures are seen. The lethality and pupariation defects seen in Cam7/Camn339 animals are not rescued by expression of CamScer\UAS.cWa under the control of Scer\GAL4elav.PLu or Scer\GAL4sca.PU. The sluggish larval locomotion of Cam7/Camn339 animals is incompletely rescued by expression of CamScer\UAS.cWa under the control of Scer\GAL4how-24B or Scer\GAL4elav.PLu. The spontaneous backward movement seen in homozygous Camn339 larvae is not rescued by expression of CamScer\UAS.cWa under the control of Scer\GAL4how-24B or Scer\GAL4sca.PU. The spontaneous backward movement seen in homozygous Camn339 larvae is rescued by expression of CamScer\UAS.cWa under the control of Scer\GAL4elav.PLu, although the larvae are still sluggish.

      (Wang et al., 2003)

      Expression of CamScer\UAS.cWa under the control of Scer\GAL4how-24B rescues the developmental defects and lethality of Cam7/Camn339 mutants.

      (Wang et al., 2002)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      CamScer\UAS.cWa
      CamUAS.cWa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Wang
      (Wang et al., 2002)
      Secondary FlyBase IDs
        References (4)