FlyBase Allele Report: Dmel\Egfr[DN.UAS]

General Information

Symbol

Dmel\Egfr^DN.UAS

Species

D. melanogaster

Name

dominant negative

FlyBase ID

FBal0058990

Feature type

allele

Associated gene

Dmel\Egfr

Associated Insertion(s)

Carried in Construct

P{UAS-Egfr.DN}

Also Known As

UAS-EGFR^DN, UAS-DN-DER, UAS-DN-EGFR, UAS-EgfrDN, EGFR^DN, UAS-DER^DN, UAS-EGFR.DN, UAS-DNDER, DER^DN, UAS-DNEgfr

Key Links

Transgenic product class

dominant_negative_variant

(O'Keefe et al., 1997)

inframe_deletion

(O'Keefe et al., 1997)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

dominant_negative_variant

(O'Keefe et al., 1997)

inframe_deletion

(O'Keefe et al., 1997)

Mutagen

in vitro construct

(O'Keefe et al., 1997, Szuts et al., 1997, Freeman, 1996)

Progenitor genotype

Carried in construct

P{UAS-Egfr.DN}

Cytology

Description

UASt regulatory sequences drive expression of a dominant-negative form of Egfr that consists of a type I Egfr cDNA with a termination codon 13 amino acids C-terminal to the transmembrane domain.

(O'Keefe et al., 1997)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Egfr

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates gastrointestinal system cancer

modeled by Apc2^g10, Apc^Q8

(Wang et al., 2013)

ameliorates colorectal cancer

modeled by Src64B^UAS.cCa

(Cordero et al., 2014)

ameliorates carcinoma

modeled by scrib¹

(Brumby and Richardson, 2003)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell polarity, with Scer\GAL4^hs.PB

(Brown and Freeman, 2003)

abnormal cell size, with Scer\GAL4^ccb-796

(Martin-Pena et al., 2006)

abnormal neuroanatomy, with Scer\GAL4^gcm-rA87.C

(Ting et al., 2005)

abnormal neuroanatomy | embryonic stage, with Scer\GAL4^repo

(Sepp and Auld, 2003)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^C855a

(Morante et al., 2013)

abnormal size, with Scer\GAL4^Bx-MS1096

(Lyulcheva et al., 2008)

abnormal size, with Scer\GAL4^T155

(Kopp et al., 1999)

chemical sensitive, with Scer\GAL4^Ilp2.PR

(Corl et al., 2009)

decreased cell number | embryonic stage, with Scer\GAL4^en-e16E

(Parker, 2006)

increased cell death, with Scer\GAL4^hs.PB

(Kurada and White, 1998)

increased cell death, with Scer\GAL4^prd.RG1

(Urban et al., 2004)

increased cell death | embryonic stage, with Scer\GAL4^en-e16E

(Parker, 2006)

increased cell death | second instar larval stage, with Scer\GAL4^C587

(Gilboa and Lehmann, 2006)

increased cell number, with Scer\GAL4^C587

(Gilboa and Lehmann, 2006)

increased cell number | male | third instar larval stage, with Scer\GAL4^C587

(La Marca et al., 2019)

increased cell size | embryonic stage, with Scer\GAL4^en-e16E

(Parker, 2006)

lethal, with Scer\GAL4^ple.PF

(Corl et al., 2009)

lethal, with Scer\GAL4^sca-537.4

(Held, 2002)

lethal - all die during embryonic stage, with Scer\GAL4^sca-537.4

(Chen and Chien, 1999)

partially lethal - majority die, with Scer\GAL4^GMR.PF

(Corl et al., 2009)

some die during embryonic stage, with Scer\GAL4^sca-537.4

(Chen and Chien, 1999)

visible, with Scer\GAL4^Act5C.PI

(Kumar and Moses, 2001)

visible, with Scer\GAL4^Bx-MS1096

(Egoz-Matia et al., 2011)

visible, with Scer\GAL4^GMR.PF

(Zettl et al., 2011, Lin et al., 2003)

visible, with Scer\GAL4^T155

(Kopp et al., 1999)

Phenotype Manifest In

abdominal lateral pentascolopidial chordotonal organ lch5, with Scer\GAL4^en-e16E

(Elstob et al., 2001)

antenna, with Scer\GAL4^Act5C.PI

(Kumar and Moses, 2001)

antenna & neuron | conditional ts, with Scer\GAL4^hs.PB

(Sen et al., 2004)

basiconic sensillum, with Scer\GAL4^lz-gal4

(Jhaveri et al., 2000)

basiconic sensillum, with Scer\GAL4^sca-P309

(Jhaveri et al., 2000)

Bolwig organ, with Scer\GAL4^so-7.GAL4

(Sprecher et al., 2007)

border follicle cell, with Scer\GAL4^slbo.2.6

(Duchek and Rorth, 2001, Duchek et al., 2001)

border follicle cell & filopodium, with Scer\GAL4^slbo.2.6

(Fulga and Rorth, 2002)

chordotonal organ precursor cell & ventral thoracic disc, with Scer\GAL4^sca-109-68

(zur Lage and Jarman, 1999)

coeloconic sensillum | ectopic, with Scer\GAL4^lz-gal4

(Jhaveri et al., 2000)

coeloconic sensillum | ectopic, with Scer\GAL4^sca-P309

(Jhaveri et al., 2000)

cone cell, with Scer\GAL4^hs.PB

(Freeman, 1996)

cyst cell of testis | third instar larval stage, with Scer\GAL4^C587

(La Marca et al., 2019)

denticle row 1, with Scer\GAL4^en-e16E

(O'Keefe et al., 1997)

dorsal acute muscle cell, with Scer\GAL4^twi.PB

(Carmena et al., 2002)

dorsal oblique muscle cell, with Scer\GAL4^twi.PB

(Carmena et al., 2002)

egg chorion, with Scer\GAL4^T155

(Ghiglione et al., 1999)

embryo, with Scer\GAL4^hs.PB

(Kurada and White, 1998)

embryonic/larval brain | third instar larval stage, with Scer\GAL4^C855a

(Morante et al., 2013)

embryonic/larval ganglionic tracheal branch, with Scer\GAL4^bs-23.26

(Gallio et al., 2004)

embryonic/larval oenocyte, with Scer\GAL4^en-e16E

(Elstob et al., 2001)

embryonic/larval oenocyte, with Scer\GAL4^salm-459.2

(Elstob et al., 2001)

embryonic epidermis, with Scer\GAL4^69B

(Payre et al., 1999)

embryonic epidermis, with Scer\GAL4^e22c

(Payre et al., 1999)

embryonic longitudinal glial cell, with Scer\GAL4^htl.POS

(Hidalgo et al., 2001)

embryonic midgut constriction 1, with Scer\GAL4^how-24B

(Szuts et al., 1998)

embryonic midgut constriction 2, with Scer\GAL4^how-24B

(Szuts et al., 1998)

embryonic midgut constriction 3, with Scer\GAL4^how-24B

(Szuts et al., 1998)

embryonic segment, with Scer\GAL4^en-e16E

(Parker, 2006)

embryonic trachea & cortical actin cytoskeleton, with Scer\GAL4^btl.PS

(Cela and Llimargas, 2006)

endoderm, with Scer\GAL4^48Y

(Szuts et al., 1998)

eye, with Scer\GAL4^Act5C.PI

(Kumar and Moses, 2001)

eye, with Scer\GAL4^GMR.PF

(Zettl et al., 2011, Mallik and Lakhotia, 2009, Wang et al., 2008, Lin et al., 2003)

eye-antennal disc, with Scer\GAL4^GMR.PF

(Freeman, 1996)

eye disc morphogenetic furrow, with Scer\GAL4^dpp.blk1

(Kumar and Moses, 2001)

eye photoreceptor cell, with Scer\GAL4^GMR.PF

(Freeman, 1996)

fascicle | embryonic stage, with Scer\GAL4^repo

(Sepp and Auld, 2003)

gastric caecum, with Scer\GAL4^how-24B

(Szuts et al., 1998)

germline stem cell | increased number, with Scer\GAL4^C587

(Gilboa and Lehmann, 2006)

gonad | male | third instar larval stage, with Scer\GAL4^C587

(La Marca et al., 2019)

lamina monopolar neuron L1, with Scer\GAL4^gcm-rA87.C

(Ting et al., 2005)

lamina monopolar neuron L2, with Scer\GAL4^gcm-rA87.C

(Ting et al., 2005)

lamina monopolar neuron L3, with Scer\GAL4^gcm-rA87.C

(Ting et al., 2005)

lamina monopolar neuron L4, with Scer\GAL4^gcm-rA87.C

(Ting et al., 2005)

lamina monopolar neuron L5, with Scer\GAL4^gcm-rA87.C

(Ting et al., 2005)

larval head, with Scer\GAL4^arm.PS

(Szuts et al., 1997)

larval neuroblast | third instar larval stage, with Scer\GAL4^C855a

(Morante et al., 2013)

lch5 ligament attachment cell, with Scer\GAL4^69B

(Inbal et al., 2004)

lch5 ligament attachment cell, with Scer\GAL4^sr-md710

(Inbal et al., 2004)

male germline stem cell | third instar larval stage, with Scer\GAL4^C587

(La Marca et al., 2019)

medial longitudinal fascicle, with Scer\GAL4^htl.POS

(Hidalgo et al., 2001)

mesothoracic tergum, with Scer\GAL4^{Ubx-lac1-Gal4}

(Pallavi and Shashidhara, 2003)

mesothoracic ventral denticle belt, with Scer\GAL4^arm.PS

(Szuts et al., 1997)

metathoracic ventral denticle belt, with Scer\GAL4^arm.PS

(Szuts et al., 1997)

midline glial cell, with Scer\GAL4^sli.PS

(Bergmann et al., 2002)

motor neuron | embryonic stage, with Scer\GAL4^repo

(Sepp and Auld, 2003)

ommatidium, with Scer\GAL4^hs.PB

(Brown and Freeman, 2003)

optic cartridge, with Scer\GAL4^αTub84B.PP

(Huang et al., 1998)

photoreceptor cell R1, with Scer\GAL4^hs.2sev

(Freeman, 1996)

photoreceptor cell R3, with Scer\GAL4^hs.2sev

(Freeman, 1996)

photoreceptor cell R4, with Scer\GAL4^hs.2sev

(Freeman, 1996)

photoreceptor cell R6, with Scer\GAL4^hs.2sev

(Freeman, 1996)

photoreceptor cell R7, with Scer\GAL4^hs.2sev

(Freeman, 1996)

photoreceptor cell R7, with Scer\GAL4^hs.PB

(Freeman, 1996)

photoreceptor neuron, with Scer\GAL4^GMR.PF

(Li and Carthew, 2005)

pleurum, with Scer\GAL4^T155

(Kopp et al., 1999)

presumptive embryonic/larval peripheral nervous system, with Scer\GAL4^repo

(Sepp and Auld, 2003)

primary pigment cell, with Scer\GAL4^hs.PB

(Freeman, 1996)

retina, with Scer\GAL4^GMR.PF

(Robertson et al., 2000, Freeman, 1996)

secondary pigment cell, with Scer\GAL4^hs.PB

(Freeman, 1996)

sensory neuron & axon & embryo, with Scer\GAL4^repo

(Sepp and Auld, 2003)

sternite, with Scer\GAL4^T155

(Kopp et al., 1999)

symmetrical commissure, with Scer\GAL4^sim.PS

(Scholz et al., 1997, Scholz et al., 1997)

tarsal segment, with Scer\GAL4^Dll-md23

(Held, 2002)

tergite, with Scer\GAL4^T155

(Kopp et al., 1999)

tertiary pigment cell, with Scer\GAL4^hs.PB

(Freeman, 1996)

tibia, with Scer\GAL4^Dll-md23

(Held, 2002)

tracheal branch primordium, with Scer\GAL4^btl.PS

(Cela and Llimargas, 2006)

trichoid sensillum, with Scer\GAL4^lz-gal4

(Jhaveri et al., 2000)

trichoid sensillum, with Scer\GAL4^sca-P309

(Jhaveri et al., 2000)

ventral denticle belt, with Scer\GAL4^69B

(Payre et al., 1999)

ventral denticle belt, with Scer\GAL4^arm.PS

(Szuts et al., 1997)

ventral denticle belt, with Scer\GAL4^e22c

(Payre et al., 1999)

ventral denticle belt, with Scer\GAL4^prd.RG1

(Urban et al., 2004)

ventral thoracic disc primordium, with Scer\GAL4^Dll-md23

(Kubota et al., 2000)

wing, with Scer\GAL4^Bx-MS1096

(Lyulcheva et al., 2008)

wing, with Scer\GAL4^{Ubx-lac1-Gal4}

(Pallavi and Shashidhara, 2003)

wing cell

(Yan et al., 2009)

wing disc dorsal compartment, with Scer\GAL4^A9

(Nagaraj et al., 1999)

wing vein

(Yan et al., 2009)

wing vein, with Scer\GAL4^GMR.PF

(Charroux et al., 2006)

wing vein L2, with Scer\GAL4^GMR.PF

(Robertson et al., 2000)

wing vein L3, with Scer\GAL4^Bx-MS1096

(Egoz-Matia et al., 2011)

wing vein L3, with Scer\GAL4^GMR.PF

(Robertson et al., 2000)

wing vein L4, with Scer\GAL4^Bx-MS1096

(Egoz-Matia et al., 2011)

wing vein L4, with Scer\GAL4^GMR.PF

(Robertson et al., 2000)

wing vein L5, with Scer\GAL4^Bx-MS1096

(Egoz-Matia et al., 2011)

wing vein L5, with Scer\GAL4^GMR.PF

(Robertson et al., 2000)

Detailed Description

Statement

Reference

Expressing Egfr^DN.UAS under the control of Scer\GAL4^C587 induces non-enclosing somatic cells and supernumerary early germline cells in L3 male gonads.

(La Marca et al., 2019)

Expression of Egfr^DN.Scer\UAS under the control of Scer\GAL4^slbo.2.6 does not significantly alter the protrusion and retraction morphodynamics in border cell cluster migration.

(Cai et al., 2014)

Scer\GAL4^C855a-mediated expression of Egfr^DN.Scer\UAS results in reduction in neuroepithelium size and formation of fewer neuroblasts.

(Morante et al., 2013)

Expression of Egfr^DN.Scer\UAS under the control of Scer\GAL4^Bx-MS1096 results in a partial loss of wing veins L3, L4 and L5.

(Egoz-Matia et al., 2011)

Expression of Egfr^DN.Scer\UAS under the control of Scer\GAL4^slbo.2.6 has little effect on the speed of net cluster movement in the early phase of posterior border cell migration, but movement in the late phase is reduced compared to controls, enhancing the difference between the two phases. The mutant cells show increased tumbling in the late phase compared to controls and the number of cellular extensions from the front of the border cell cluster is reduced compared to wild type.

(Poukkula et al., 2011)

Expression of Egfr^DN.Scer\UAS under the control of Scer\GAL4^GMR.PF produces a rough eye phenotype.

(Zettl et al., 2011)

Expression of Egfr^DN.Scer\UAS under the control of Scer\GAL4^Ilp2.PR results in flies with increased sensitivity to ethanol-induced sedation compared to control flies.

(Corl et al., 2009)

Expression of Egfr^DN.Scer\UAS under the control of Scer\GAL4^P2.4.Pdf prevents increases in sleep after social enrichment.

(Donlea et al., 2009)

Scer\GAL4^GMR.PF-mediated expression of Egfr^DN.Scer\UAS results in eyes that are severely reduced in size and mostly devoid of ommatidial organization.

(Mallik and Lakhotia, 2009)

Flip-out clones expressing Egfr^DN.Scer\UAS in the wing, under the control of Scer\GAL4^Act5C.PI interrupts wing veins LE, L3, and L4.

(Yan et al., 2009)

Expression of Egfr^DN.Scer\UAS in the wing using Scer\GAL4^Bx-MS1096 results in dramatically reduced, narrow wings and loss of wing veins.

(Lyulcheva et al., 2008)

Expression of Egfr^DN.Scer\UAS under the control of Scer\GAL4^GMR.PF results in a small eye phenotype.

(Wang et al., 2008)

Animals expressing Egfr^DN.Scer\UAS under the control of Scer\GAL4^so-7.GAL4 develop only 3 or 4 immature photoreceptors in the Bolwig's organ compared to approximately 12 cells in the wild type. The remaining photoreceptors in the mutant larvae are all of the Rh5 subtype, no Rh6 photoreceptors are present.

(Sprecher et al., 2007)

The tracheal systems of embryos that express Egfr^DN.Scer\UAS under the control of Scer\GAL4^btl.PS show branch interruptions and branches with cells only connected by cytoplasmic extensions. This phenotype has a 96% penetrance. The general pattern and outgrowth of dorsal and ganglionic branches is not grossly affected.

The tracheal tubes of stage 16 Scer\GAL4^btl.PS>Egfr^DN.Scer\UAS embryos show a thinner accumulation of cortical actin compared to controls.

(Cela and Llimargas, 2006)

Expression of Egfr^DN.Scer\UAS, under the control of Scer\GAL4^GMR.PF, suppresses wing vein formation in the wing.

(Charroux et al., 2006)

There is a large increase in primordial germ cell numbers when the dominant negative Egfr^DN.Scer\UAS is expressed in somatic gonadal cells by Scer\GAL4^C587.

Fewer intermingled cells (somatic cells adjacent to primordial germ cells) are present in Egfr^DN.Scer\UAS mutants (driven by Scer\GAL4^C587) gonads compared to wild-type.

There is a significant increase in apoptosis in late 2nd instar larva expressing Egfr^DN.Scer\UAS under the control of Scer\GAL4^C587, compared to larva expressing Scer\GAL4^C587 alone. Other aspects of somatic differentiation and morphogenesis seem normal in these mutants.

(Gilboa and Lehmann, 2006)

Overexpression of Egfr^DN.Scer\UAS under the control of Scer\GAL4⁷⁹⁶ decreases cell size but does not affect synapse number.

(Martin-Pena et al., 2006)

Expression of Egfr^DN.Scer\UAS under the control of Scer\GAL4^slbo.2.6 does not affect border cell migration.

(McDonald et al., 2006)

Expression of Egfr^DN.Scer\UAS, under the control of Scer\GAL4^en-e16E, in the posterior compartment cells of embryos results in apoptosis of these cells, beginning at stage 12. This increased cell death reduces the number of cells per posterior compartment from 44 to 30 and compartment size is reduced by 15%. The increased apoptosis occurs mainly at the front of the posterior compartment. Surviving cells are larger than normal.

(Parker, 2006)

Expression of Egfr^DN.Scer\UAS under the control of Scer\GAL4^ato.3.6 does not affect dorsal cluster neuron axon extension across the optic chiasm in adult flies.

(Srahna et al., 2006)

Ommatidia that express Egfr^DN.Scer\UAS, under the control of Scer\GAL4^GMR.PF, show differentiation of only the first two or three photoreceptors (most likely to be R8 and R2 or R5), with others failing to differentiate.

(Li and Carthew, 2005)

Expression of Egfr^DN.Scer\UAS under the control of Scer\GAL4^gcm-rA87.C blocks the differentiation of L1-L5 lamina neurons.

(Ting et al., 2005)

Hemocytes in Egfr^DN.Scer\UAS; Scer\GAL4^srp.Hemo late embryos do not exhibit abnormal aggregation.

(Bruckner et al., 2004)

Migration many ganglionic branches approaching the midline during stage 16 is defective in Egfr^DN.Scer\UAS; Scer\GAL4^bs-23.26 embryos: 9% of branches are stalled and 25% misrouted, 5% cross the midline and 3% arrest upon reaching it.

(Gallio et al., 2004)

lch5 ligament attachment cells are not formed in Egfr^DN.Scer\UAS; Scer\GAL4^69B or Egfr^DN.Scer\UAS; Scer\GAL4^sr-md710 embryos. lch5 cap attachment cells, however, are unaffected.

(Inbal et al., 2004)

When Egfr^DN.Scer\UAS is driven by Scer\GAL4^hs.PB and heatshocked an alteration of the fascicular pattern of the sensory afferents from the antenna are seen.

(Sen et al., 2004)

P{UAS-Egfr.DN}29-77-1; Scer\GAL4^prd.RG1 embryos do not have obvious cuticle phenotypes. However, P{UAS-Egfr.DN}1-7; Scer\GAL4^prd.RG1 embryo cuticles have in strong denticle belt fusions in alternating parasegments, centred on the midline and extending up to 2/3 of the width of each denticle belt. From stages 10-12 there is a dramatic increase in apoptosis in prd expression domains in the developing epidermis of these animals. Increased apoptosis persists at the midline through stages 13 and 14.

(Urban et al., 2004)

When Egfr^DN.Scer\UAS is driven by Scer\GAL4^hs.PB adults exhibit misrotated ommatidia.

(Brown and Freeman, 2003)

Expression of Egfr^DN.Scer\UAS under the control of Scer\GAL4^GMR.PF results in a small eye phenotype.

(Lin et al., 2003)

Egfr^DN.Scer\UAS; Scer\GAL4^{Ubx-lac1-Gal4} adults exhibit wing duplication in the posterior compartment and partial loss of notum and/or hinge. Occasionally pharate adults die within the pupal case, with severe loss of notum tissue.

(Pallavi and Shashidhara, 2003)

Expression of Egfr^DN.Scer\UAS in embryonic glia, under the control of Scer\GAL4^repo, causes PNS patterning defects in embryonic hemisegments. In hemisegments that are moderately affected, sensory axons can be misdirected and nerves in the CNS-PNS transition zone show incorrect fasciculation-bundling. In severely-affected hemisegments, there is a loss of sensory neurons and those remaining have misplaced cell bodies and misdirected sensory axons. Additionally, motor neuron patterning is disrupted.

(Sepp and Auld, 2003)

In Egfr^DN.Scer\UAS; Scer\GAL4^sli.PS embryos midline glial cells form normally, but only a few survive to the end of embryogenesis.

(Bergmann et al., 2002)

When Egfr^DN.Scer\UAS is driven by Scer\GAL4^twi.PB a reduction in the number of DA1 and DO2 muscles.

(Carmena et al., 2002)

Border cells in the ovaries of Egfr^DN.Scer\UAS; Scer\GAL4^slbo.2.6 animals form significantly fewer long cellular extensions (20-40 μm) and intermediate cellular extensions (10-20 μm).

(Fulga and Rorth, 2002)

Expression of btl::Egfr^{Scer\UAS.T:λ\cI-DD} under the control of Scer\GAL4^sca-537.4 or Scer\GAL4^Dll-md23 has no detectable effect on bract formation. When driven by Scer\GAL4^sca-537.4 other defects are seen. Tarsal segments 2-4 are shortened and fused, and the entire tarsus is reduced to a bump on the end of a swollen tibia. When driven by Scer\GAL4^Dll-md23, no adult survivors are seen.

(Held, 2002)

Dorsal migration of border cells is severely inhibited when Egfr^DN.Scer\UAS is expressed under the control of either Scer\GAL4^slbo.2.6, with only minor effects on the initial posterior migration. Most eggs derived from these females do not hatch.

(Duchek and Rorth, 2001)

When Egfr^DN.Scer\UAS is driven by Scer\GAL4^slbo.2.6 a mild effect on border cell migration is seen.

(Duchek et al., 2001)

Expression of Egfr^DN.Scer\UAS under the control of Scer\GAL4^en-e16E at 25^oC or 29^oC results in a complete loss of oenocytes. At 25^oC, the number of chordotonal organs in the lateral cluster is reduced from 5 to 4 in these embryos, while at 29^oC the number is reduced from 5 to 3. Expression of Egfr^DN.Scer\UAS under the control of Scer\GAL4^salm-459.2 at 29^oC has no effect on chordotonal organ number but does result in a significant reduction in the number of oenocytes per cluster.

(Elstob et al., 2001)

The first longitudinal fascicle in the embryonic central nervous system of Egfr^DN.Scer\UAS; Scer\GAL4^elav.PLu embryos is misrouted across the midline, but the second and third longitudinal fascicles are normal. Development of the longitudinal (interface) glial cells is normal. Egfr^DN.Scer\UAS; Scer\GAL4^htl.POS embryos exhibit increased apoptosis of longitudinal (interface) glial cells.

(Hidalgo et al., 2001)

Flies expressing Egfr^DN.Scer\UAS under the control of Scer\GAL4^Act5C.PI lack the eye and antenna.

(Kumar and Moses, 2001)

Expression of Egfr^DN.Scer\UAS under the control of Scer\GAL4^dpp.blk1 inhibits furrow reincarnation along the lateral margins, but not furrow birth at the posterior of the eye disc.

(Kumar and Moses, 2001)

Neuronal patterning in the ommatidial preclusters is maintained in flies expressing Egfr^DN.Scer\UAS under the control of Scer\GAL4^lz-gal4.

(Flores et al., 2000)

When Egfr^DN.Scer\UAS is driven by Scer\GAL4^sca-P309 or Scer\GAL4^lz-gal4 a significant increase in coeloconic sensilla and a concomitant reduction of basiconica and trichoidea is seen on the antennal surface of these animals.

(Jhaveri et al., 2000)

Expression of Egfr^DN.Scer\UAS under the control of Scer\GAL4^Dll-md23 interferes with leg disc development in embryos, while leaving the wing disc intact.

(Kubota et al., 2000)

Expression of Egfr^DN.Scer\UAS under the control of Scer\GAL4^15J2 does not result in consistent errors in axon trajectory or longitudinal tract establishment.

(Lanoue et al., 2000)

Expression of Egfr^DN.Scer\UAS under the control of Scer\GAL4^GMR.PF leads to complete loss of the adult retina. In animals raised at 29[o]C, veins L2, L3, L4 and L5 are disrupted.

(Robertson et al., 2000)

Most animals expressing Egfr^DN.Scer\UAS under the control of Scer\GAL4^sca-537.4 die as embryos, except for a few survivors to the larval stage.

(Chen and Chien, 1999)

Eggs derived from females expressing Egfr^DN.Scer\UAS under the control of Scer\GAL4^T155 show a ventralisation of the eggshell.

(Ghiglione et al., 1999)

When expression is driven by Scer\GAL4^T155 the pleura expands at the expense of the sternites and tergites.

(Kopp et al., 1999)

Expression of Egfr^DN.Scer\UAS under the control of Scer\GAL4^A9 results in a dramatic reduction in the dorsal compartment of the wing pouch in the wing disc.

(Nagaraj et al., 1999)

Egfr^DN.Scer\UAS driven by either Scer\GAL4^e22c or Scer\GAL4^69B results in extra naked cuticle in otherwise wild-type embryos.

(Payre et al., 1999)

Expression of Egfr^DN.Scer\UAS under the control of Scer\GAL4^sca-109-68 results in a dramatic loss of chordotonal sensory organ precursors (SOPs) in late third instar imaginal leg discs. The appearance of bristle SOPs is unaffected. Expression of Egfr^DN.Scer\UAS under the control of Scer\GAL4^ase.PZ has no effect on the number of ase-expressing SOPs in the leg disc.

(zur Lage and Jarman, 1999)

When expression is driven by Scer\GAL4^αTub84B.PP in clones in the lamina, timed to permit imaginal cell proliferation and the formation of photoreceptor clusters at the posterior of the eye, an essentially normal array of photoreceptors innervate the lamin target field. However neuronal differentiation in the LPCs fails.

(Huang et al., 1998)

When expression is driven in the embryo with Scer\GAL4^hs.PB ectopic apoptosis is induced.

(Kurada and White, 1998)

When expressed under the influence of Scer\GAL4^48Y, cells in the endoderm are missing or unhealthy in the lab domain and the anterior midgut in the ps3/ps4 region. The midgut epithelium is largely intact. When expressed under the influence of Scer\GAL4^how-24B, midgut constrictions are abnormal and the gastric caeca are stunted.

(Szuts et al., 1998)

DA1 muscle precursor formation is either normal or affected in not more than one segment in embryos expressing Egfr^DN.Scer\UAS under the control of Scer\GAL4^how-24B at 22^oC.

(Yarnitzky et al., 1998)

Embryos expressing Egfr^DN.Scer\UAS under the control of Scer\GAL4^en-e16E lack the first denticle row.

(O'Keefe et al., 1997)

Scer\GAL4^sli.PS-mediated expression in wild type embryos does not result in an abnormal CNS phenotype. Scer\GAL4^sim.PS-mediated expression causes a weak fused commissure phenotype.

(Scholz et al., 1997, Scholz et al., 1997)

Produces severely mutant larvae that do not hatch from their vitelline membranes when expressed ubiquitously in embryos using Scer\GAL4^arm.PS. These larvae have head defects and very narrow denticle belts. The abdominal belts usually consist of approximately 4 disordered rows of denticles, which are tapered and are less hooked compared to wild-type. The belts normally contain one or two anterior rows with a few largish denticles, followed by a strip of tiny denticles. The denticles correspond to row 5 and 6 denticles. The second and third thoracic denticle belts also lack most of their denticles, although the prothoracic denticle belt and beard appear normal.

(Szuts et al., 1997)

Scer\GAL4^GMR.PF induced expression causes complete loss of the adult retina, some interommatidial bristles remain. Photoreceptor development is disrupted and the eye-antennal disc shows an overall disorganisation. Scer\GAL4^hs.PB induced expression prevents receptor cell R7 in some ommatidia from forming. Early pupal heat shocks causes ommatidia that have 2, instead of 4 cone cells, and are depleted of primary, secondary and tertiary pigment cells, independent of the number of photoreceptor cells. Scer\GAL4^hs.2sev induced expression prevents receptor cells R1, R3, R4, R6 and R7 from forming.

(Freeman, 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Egfr^DN.UAS, Scer\GAL4^GMR.PF has partially lethal - majority die phenotype, enhanceable by hppy^RB.UAS, Scer\GAL4^GMR.PF

(Corl et al., 2009)

NOT Enhanced by

Statement

Reference

Egfr^DN.UAS, Scer\GAL4^C587 has increased cell number | third instar larval stage | male phenotype, non-enhanceable by Strip[+]/Strip^jam

(La Marca et al., 2019)

Suppressed by

Statement

Reference

Egfr^DN.UAS, Scer\GAL4^GMR.PF has visible phenotype, suppressible | partially by rho-5^KO1

(Zettl et al., 2011)

Egfr^DN.UAS, Scer\GAL4^GMR.PF has visible phenotype, suppressible by spen^DN.UAS, Scer\GAL4^GMR.PF

(Lin et al., 2003)

Enhancer of

Statement

Reference

Egfr^DN.UAS, Scer\GAL4^en-e16E is an enhancer of increased cell death | embryonic stage phenotype of CycE^UAS.cLa, Scer\GAL4^en-e16E

(Parker, 2006)

Suppressor of

Statement

Reference

Egfr^DN.UAS, Scer\GAL4^NP5130 is a suppressor of hyperplasia phenotype of Scer\GAL4^NP5130, Src64B^UAS.cCa

(Cordero et al., 2014)

Scer\GAL4^Act.PU/Egfr^DN.UAS is a suppressor of hyperplasia | somatic clone phenotype of Apc2^g10, Apc^Q8

(Wang et al., 2013)

Other

Statement

Reference

Egfr^DN.UAS, Pvr^DN.UASp, Scer\GAL4^slbo.2.6 has abnormal cell migration phenotype

(Cai et al., 2014)

Egfr^DN.UAS, Pvr^DN.UASp, Rac1^N17.UAS, Scer\GAL4^slbo.2.6 has abnormal cell migration phenotype

(Cai et al., 2014)

Egfr^DN.UAS, Rac1^N17.UAS, Scer\GAL4^slbo.2.6 has abnormal cell migration phenotype

(Cai et al., 2014)

Egfr^DN.UAS, Pvr^DN.UASp, Scer\GAL4^slbo.2.6, Tie^DN.UAS has abnormal cell migration phenotype

(Cai et al., 2014)

BacA\p35^UAS.cHa, Egfr^DN.UAS, Scer\GAL4^en-e16E has decreased cell death phenotype

(Parker, 2006)

Egfr^DN.UAS, Scer\GAL4^T155, pan^ΔN.UAS has visible phenotype

(Kopp et al., 1999)

Egfr^DN.UAS, Scer\GAL4^T155, dpp^UAS.cSa has visible phenotype

(Kopp et al., 1999)

Egfr^DN.UAS, Scer\GAL4^T155, dpp^UAS.cSa has abnormal size phenotype

(Kopp et al., 1999)

Phenotype Manifest In

Enhanced by

Statement

Reference

Egfr^DN.UAS, Pvr^DN.UASp, Scer\GAL4^slbo.2.6 has border follicle cell phenotype, enhanceable by Tie^DN.UAS, Scer\GAL4^slbo.2.6

(Wang et al., 2006)

Egfr^DN.UAS, Scer\GAL4^slbo.2.6 has border follicle cell & filopodium phenotype, enhanceable by Pvr^DN.UASp, Scer\GAL4^slbo.2.6

(Fulga and Rorth, 2002)

Egfr^DN.UAS, Scer\GAL4^slbo.2.6 has border follicle cell phenotype, enhanceable by Pvr^DN.UASp, Scer\GAL4^slbo.2.6

(Duchek et al., 2001)

Egfr^DN.UAS, Scer\GAL4^slbo.2.6 has border follicle cell phenotype, enhanceable by Pvf1^EP1624, Scer\GAL4^slbo.2.6

(Duchek et al., 2001)

NOT Enhanced by

Statement

Reference

Egfr^DN.UAS, Scer\GAL4^C587 has gonad | third instar larval stage | male phenotype, non-enhanceable by Strip[+]/Strip^jam

(La Marca et al., 2019)

Egfr^DN.UAS, Scer\GAL4^C587 has male germline stem cell | third instar larval stage phenotype, non-enhanceable by Strip[+]/Strip^jam

(La Marca et al., 2019)

Egfr^DN.UAS, Scer\GAL4^C587 has cyst cell of testis | third instar larval stage phenotype, non-enhanceable by Strip[+]/Strip^jam

(La Marca et al., 2019)

Suppressed by

Statement

Reference

Egfr^DN.UAS, Scer\GAL4^GMR.PF has eye phenotype, suppressible | partially by rho-5^KO1

(Zettl et al., 2011)

Egfr^DN.UAS, Scer\GAL4^hs.PB has wing vein phenotype, suppressible by Egfr^DN.UAS, Scer\GAL4^hs.PB

(Yan et al., 2009)

Egfr^DN.UAS, Scer\GAL4^hs.PB has wing cell phenotype, suppressible by Egfr^DN.UAS, Scer\GAL4^hs.PB

(Yan et al., 2009)

Egfr^DN.UAS, Scer\GAL4^GMR.PF has eye phenotype, suppressible by Cbl^K26

(Wang et al., 2008)

Egfr^DN.UAS, Scer\GAL4^GMR.PF has eye phenotype, suppressible by spen^DN.UAS, Scer\GAL4^GMR.PF

(Lin et al., 2003)

Egfr^DN.UAS, Scer\GAL4^sli.PS has midline glial cell phenotype, suppressible by hid^rvX1/hid^rvX1

(Bergmann et al., 2002)

Egfr^DN.UAS, Scer\GAL4^twi.PB has dorsal oblique muscle cell phenotype, suppressible by Delta^X

(Carmena et al., 2002)

Egfr^DN.UAS, Scer\GAL4^twi.PB has pericardial cell phenotype, suppressible by Delta^X

(Carmena et al., 2002)

Egfr^DN.UAS, Scer\GAL4^twi.PB has pericardial cell phenotype, suppressible by aos^Δ7

(Carmena et al., 2002)

Egfr^DN.UAS, Scer\GAL4^twi.PB has dorsal oblique muscle cell phenotype, suppressible by aos^Δ7

(Carmena et al., 2002)

Egfr^DN.UAS, Scer\GAL4^GMR.PF has retina phenotype, suppressible by Cbl^Dv.UAS, Scer\GAL4^GMR.PF

(Robertson et al., 2000)

Egfr^DN.UAS, Scer\GAL4^GMR.PF has wing vein L2 phenotype, suppressible by Cbl^Dv.UAS, Scer\GAL4^GMR.PF

(Robertson et al., 2000)

Egfr^DN.UAS, Scer\GAL4^GMR.PF has wing vein L3 phenotype, suppressible by Cbl^Dv.UAS, Scer\GAL4^GMR.PF

(Robertson et al., 2000)

Egfr^DN.UAS, Scer\GAL4^GMR.PF has wing vein L4 phenotype, suppressible by Cbl^Dv.UAS, Scer\GAL4^GMR.PF

(Robertson et al., 2000)

Egfr^DN.UAS, Scer\GAL4^GMR.PF has wing vein L5 phenotype, suppressible by Cbl^Dv.UAS, Scer\GAL4^GMR.PF

(Robertson et al., 2000)

Egfr^DN.UAS, Scer\GAL4^69B has embryonic epidermis phenotype, suppressible by ovo^UAS.cPa, Scer\GAL4^69B

(Payre et al., 1999)

Egfr^DN.UAS, Scer\GAL4^69B has ventral denticle belt phenotype, suppressible by ovo^UAS.cPa, Scer\GAL4^69B

(Payre et al., 1999)

Egfr^DN.UAS, Scer\GAL4^e22c has embryonic epidermis phenotype, suppressible by ovo^UAS.cPa, Scer\GAL4^e22c

(Payre et al., 1999)

Egfr^DN.UAS, Scer\GAL4^e22c has ventral denticle belt phenotype, suppressible by ovo^UAS.cPa, Scer\GAL4^e22c

(Payre et al., 1999)

NOT suppressed by

Statement

Reference

Egfr^DN.UAS, Scer\GAL4^GMR.PF has eye phenotype, non-suppressible by lncRNA:Hsrω^RNAi.Sym.UAS, Scer\GAL4^GMR.PF

(Mallik and Lakhotia, 2009)

Egfr^DN.UAS, Scer\GAL4^btl.PS has tracheal branch primordium phenotype, non-suppressible by BacA\p35^UAS.cHa, Scer\GAL4^btl.PS

(Cela and Llimargas, 2006)

Enhancer of

Statement

Reference

Egfr^DN.UAS, Scer\GAL4^slbo.2.6 is an enhancer of border follicle cell phenotype of Pvr^DN.UASp, Scer\GAL4^slbo.2.6

(McDonald et al., 2006, Duchek et al., 2001)

Egfr^DN.UAS, Scer\GAL4^slbo.2.6 is an enhancer of border follicle cell & filopodium phenotype of Pvr^DN.UASp, Scer\GAL4^slbo.2.6

(Fulga and Rorth, 2002)

Scer\GAL4^how-24B/Egfr^DN.UAS is an enhancer of muscle cell of abdominal 6 dorsal acute muscle 1 | precursor phenotype of vn¹⁰⁵⁶⁷

(Yarnitzky et al., 1998)

Scer\GAL4^how-24B/Egfr^DN.UAS is an enhancer of muscle cell of abdominal 7 dorsal acute muscle 1 | precursor phenotype of vn¹⁰⁵⁶⁷

(Yarnitzky et al., 1998)

Scer\GAL4^how-24B/Egfr^DN.UAS is an enhancer of muscle cell of abdominal 2 dorsal acute muscle 1 | precursor phenotype of vn^L6/Df(3L)XAS96

(Yarnitzky et al., 1998)

Scer\GAL4^how-24B/Egfr^DN.UAS is an enhancer of muscle cell of abdominal 3 dorsal acute muscle 1 | precursor phenotype of vn^L6/Df(3L)XAS96

(Yarnitzky et al., 1998)

Scer\GAL4^how-24B/Egfr^DN.UAS is an enhancer of muscle cell of abdominal 4 dorsal acute muscle 1 | precursor phenotype of vn^L6/Df(3L)XAS96

(Yarnitzky et al., 1998)

Scer\GAL4^how-24B/Egfr^DN.UAS is an enhancer of muscle cell of abdominal 5 dorsal acute muscle 1 | precursor phenotype of vn^L6/Df(3L)XAS96

(Yarnitzky et al., 1998)

Scer\GAL4^how-24B/Egfr^DN.UAS is an enhancer of muscle cell of abdominal 6 dorsal acute muscle 1 | precursor phenotype of vn^L6/Df(3L)XAS96

(Yarnitzky et al., 1998)

Scer\GAL4^how-24B/Egfr^DN.UAS is an enhancer of muscle cell of abdominal 7 dorsal acute muscle 1 | precursor phenotype of vn^L6/Df(3L)XAS96

(Yarnitzky et al., 1998)

Scer\GAL4^how-24B/Egfr^DN.UAS is an enhancer of muscle cell of abdominal 2 dorsal acute muscle 1 | precursor phenotype of vn¹⁰⁵⁶⁷

(Yarnitzky et al., 1998)

Scer\GAL4^how-24B/Egfr^DN.UAS is an enhancer of muscle cell of abdominal 3 dorsal acute muscle 1 | precursor phenotype of vn¹⁰⁵⁶⁷

(Yarnitzky et al., 1998)

Scer\GAL4^how-24B/Egfr^DN.UAS is an enhancer of muscle cell of abdominal 4 dorsal acute muscle 1 | precursor phenotype of vn¹⁰⁵⁶⁷

(Yarnitzky et al., 1998)

Scer\GAL4^how-24B/Egfr^DN.UAS is an enhancer of muscle cell of abdominal 5 dorsal acute muscle 1 | precursor phenotype of vn¹⁰⁵⁶⁷

(Yarnitzky et al., 1998)

Suppressor of

Statement

Reference

Egfr^DN.UAS, Scer\GAL4^NP5130 is a suppressor of intestinal stem cell of posterior adult midgut epithelium phenotype of Scer\GAL4^NP5130, Src64B^UAS.cCa

(Cordero et al., 2014)

Egfr^DN.UAS/Scer\GAL4^hs.2sev is a suppressor of eye phenotype of Scer\GAL4^GMR.PU, miple1^UAS.Tag:HA

(Muñoz-Soriano et al., 2013)

Egfr^DN.UAS, Scer\GAL4^hs.2sev is a suppressor of eye phenotype of Scer\GAL4^hs.2sev, miple1^UAS.Tag:HA

(Muñoz-Soriano et al., 2013)

Scer\GAL4^Act.PU/Egfr^DN.UAS is a suppressor of adult midgut | somatic clone phenotype of Apc2^g10, Apc^Q8

(Wang et al., 2013)

Scer\GAL4^Act.PU/Egfr^DN.UAS is a suppressor of intestinal stem cell | somatic clone phenotype of Apc2^g10, Apc^Q8

(Wang et al., 2013)

Scer\GAL4^Mef2.PR/Egfr^DN.UAS is a suppressor of muscle attachment site | ectopic phenotype of sli²

(Deng et al., 2010)

Egfr^DN.UAS, Scer\GAL4^e22c is a suppressor of wing vein phenotype of Scer\GAL4^e22c, tkv^{Q253D.UAS.cNb}

(Yan et al., 2009)

Egfr^DN.UAS, Scer\GAL4^e22c is a suppressor of wing cell phenotype of Scer\GAL4^e22c, tkv^{Q253D.UAS.cNb}

(Yan et al., 2009)

Egfr^DN.UAS, Scer\GAL4^hs.PB is a suppressor of wing vein phenotype of Scer\GAL4^hs.PB, tkv^{Q253D.UAS.cNb}

(Yan et al., 2009)

Egfr^DN.UAS, Scer\GAL4^hs.PB is a suppressor of wing cell phenotype of Scer\GAL4^hs.PB, tkv^{Q253D.UAS.cNb}

(Yan et al., 2009)

Egfr^DN.UAS, Scer\GAL4^hs.PB is a suppressor of wing vein phenotype of Egfr^DN.UAS, Scer\GAL4^hs.PB

(Yan et al., 2009)

Egfr^DN.UAS, Scer\GAL4^hs.PB is a suppressor of wing cell phenotype of Egfr^DN.UAS, Scer\GAL4^hs.PB

(Yan et al., 2009)

Egfr^DN.UAS, Scer\GAL4^Bx-MS1096 is a suppressor of wing phenotype of Scer\GAL4^Bx-MS1096, pico^{UAS.Tag:polyHis,Tag:MYC}

(Lyulcheva et al., 2008)

Egfr^DN.UAS, Scer\GAL4^GMR.PF is a suppressor of wing vein | ectopic phenotype of Hsap\ATN1^N917.65Q.UAS, Scer\GAL4^GMR.PF

(Charroux et al., 2006)

Scer\GAL4^twi.PB/Egfr^DN.UAS is a suppressor of dorsal acute muscle cell | ectopic phenotype of aos^Δ7

(Carmena et al., 2002)

Scer\GAL4^twi.PB/Egfr^DN.UAS is a suppressor of dorsal acute muscle cell | precursor | ectopic phenotype of aos^Δ7

(Carmena et al., 2002)

Scer\GAL4^twi.PB/Egfr^DN.UAS is a suppressor of dorsal oblique muscle cell | ectopic phenotype of aos^Δ7

(Carmena et al., 2002)

Scer\GAL4^twi.PB/Egfr^DN.UAS is a suppressor of dorsal oblique muscle cell | precursor | ectopic phenotype of aos^Δ7

(Carmena et al., 2002)

Scer\GAL4^twi.PB/Egfr^DN.UAS is a suppressor of pericardial cell | ectopic phenotype of aos^Δ7

(Carmena et al., 2002)

Scer\GAL4^twi.PB/Egfr^DN.UAS is a suppressor of pericardial cell | precursor | ectopic phenotype of aos^Δ7

(Carmena et al., 2002)

Egfr^DN.UAS, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Cbl^Dv.UAS, Scer\GAL4^GMR.PF

(Robertson et al., 2000)

Egfr^DN.UAS, Scer\GAL4^GMR.PF is a suppressor of photoreceptor cell R7 phenotype of Cbl^Dv.UAS, Scer\GAL4^GMR.PF

(Robertson et al., 2000)

Egfr^DN.UAS, Scer\GAL4^GMR.PF is a suppressor of wing vein phenotype of Cbl^Dv.UAS, Scer\GAL4^GMR.PF

(Robertson et al., 2000)

Egfr^DN.UAS, Scer\GAL4^how-24B is a suppressor | partially of embryonic midgut constriction phenotype of Scer\GAL4^how-24B, dpp^UAS.cSa

(Szuts et al., 1998)

NOT Suppressor of

Statement

Reference

Scer\GAL4^arm.PS/Egfr^DN.UAS is a non-suppressor of denticle phenotype of wg^l-17

(Szuts et al., 1997)

Other

Statement

Reference

Egfr^DN.UAS, Pvr^DN.UASp, Rac1^N17.UAS, Scer\GAL4^slbo.2.6 has border follicle cell phenotype

(Cai et al., 2014)

Egfr^DN.UAS, Rac1^N17.UAS, Scer\GAL4^slbo.2.6 has border follicle cell phenotype

(Cai et al., 2014)

Egfr^DN.UAS, Pvr^DN.UASp, Scer\GAL4^slbo.2.6, Tie^DN.UAS has border follicle cell phenotype

(Cai et al., 2014)

Egfr^DN.UAS, Pvr^DN.UASp, Scer\GAL4^slbo.2.6 has border follicle cell phenotype

(Cai et al., 2014, Inaki et al., 2012, Wang et al., 2006)

BacA\p35^UAS.cHa, Egfr^DN.UAS, Scer\GAL4^en-e16E has embryonic segment phenotype

(Parker, 2006)

Egfr^DN.UAS, Scer\GAL4^GMR.PF, mir-7^UAS.cLb has photoreceptor neuron | ectopic phenotype

(Li and Carthew, 2005)

Egfr^DN.UAS, Scer\GAL4^T155, pan^ΔN.UAS has tergite phenotype

(Kopp et al., 1999)

Egfr^DN.UAS, Scer\GAL4^T155, dpp^UAS.cSa has tergite phenotype

(Kopp et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

Flies expressing Egfr^DN.Scer\UAS and Pvr^DN.Scer\UAS under the control of Scer\GAL4^slbo.2.6 show differences in the migration behaviour of border cell clusters. First, a distinct front-back polarity is evident in wild-type clusters, which show spatially segregated protrusion and retraction with high protrusion velocities predominating at the front and high retraction velocities at the back. This spatial segregation is lost in clusters lacking guidance-receptor activities. Second, these double mutant clusters display overall slower protrusion and retraction velocities.

Flies expressing Rac1^N17.Scer\UAS, Pvr^DN.Scer\UAS and Egfr^DN.Scer\UAS exhibit a change in border cell migration, with changes in local protrusion and retraction behaviour that are different to those found in Rac1^N17.Scer\UAS single mutants.

Flies expressing Rac1^N17.Scer\UAS and Egfr^DN.Scer\UAS exhibit a change in border cell migration, with changes in local protrusion and retraction behaviour that are different to those found in Rac1^N17.Scer\UAS single mutants.

The addition of a dominant-negative Tie^DN.Scer\UAS to border cells expressing Pvr^DN.Scer\UAS and Egfr^DN.Scer\UAS alters the protrusion and retraction morphodynamics of border cell cluster migration.

(Cai et al., 2014)

Expression of Egfr^DN.Scer\UAS under the control of Scer\GAL4^esg.PU suppresses the intestinal stem cell hyperproliferation seen in Burs^Z5569/Burs^Z5569 mutants.

(Scopelliti et al., 2014)

Downregulation of Egfr, through expression of Egfr^DN.Scer\UAS, suppresses the Scer\GAL4^GMR.PU>miple1^{Scer\UAS.T:Ivir\HA1} eye phenotype.

Downregulation of Egfr, through expression of Egfr^DN.Scer\UAS, does not alter the ommatidial rotation phenotype seen in flies expressing miple1^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^hs.2sev.

(Muñoz-Soriano et al., 2013)

Expression of Egfr^DN.Scer\UAS under the control of Scer\GAL4^Act.PU suppresses the increased size of Apc2^g10 Apc^Q8 mutant intestinal stem cell clones 7 and 14 days after clone induction (ACI). The multilayering phenotype often seen at 21 days ACI is also suppressed.

(Wang et al., 2013)

Co-expression of Pvr^DN.Scer\UAS and Egfr^DN.Scer\UAS under the control of Scer\GAL4^slbo.2.6 results in defects in border cell migration.

(Inaki et al., 2012)

Border cell clusters show severe delays in initiating posterior migration and show strongly reduced forward speed once migratory in females co-expressing Pvr^DN.Scer\UAS and Egfr^DN.Scer\UAS under the control of Scer\GAL4^slbo.2.6. The number of cellular extensions from the front of the border cell cluster is reduced compared to wild type and an increase in side and back extensions.

(Poukkula et al., 2011)

A homozygous rho-5^KO1 background suppresses the rough eye phenotype seen when Egfr^DN.Scer\UAS is expressed under the control of Scer\GAL4^GMR.PF.

(Zettl et al., 2011)

Coexpression of tkv^{Q253D.Scer\UAS.cNb} with Egfr^DN.Scer\UAS, under the control of Scer\GAL4^e22c, not only fails to induce ectopic vein cells, but also inhibits endogenous vein formation, suggesting that activation of the dpp pathway alone is not sufficient for vein differentiation.

(Yan et al., 2009)

A Cbl^K26 heterozygous background rescues the rough eye phenotype found in mutants expressing Egfr^DN.Scer\UAS under the control of Scer\GAL4^GMR.PF.

(Wang et al., 2008)

Coexpression of Egfr^DN.Scer\UAS and Pvr^DN.Scer\UAS, under the control of Scer\GAL4^slbo.2.6, results in a strong enhancement of the border cell misguidance phenotype seen when Pvr^DN.Scer\UAS is expressed alone; the penetrance of the phenotype is increased from 16 to 90%.

(McDonald et al., 2006)

Coexpression of Egfr^DN.Scer\UAS and CycE^Scer\UAS.cLa, under the control of Scer\GAL4^en-e16E, show approximately double the amount of apoptosis in cells of posterior compartments relative to expressing CycE^Scer\UAS.cLa alone.

(Parker, 2006)

Co-expression of Pvr^DN.Scer\UAS and Egfr^DN.Scer\UAS under the control of Scer\GAL4^c306 results in border cell migration defects. The severity of the migration defects is enhanced if the females also express Tie^DN.Scer\UAS.

(Wang et al., 2006)

Coexpression of the Egfr^DN.Scer\UAS and mir-7^Scer\UAS.cLb transgenes, under the control of Scer\GAL4^GMR.PF, results in ommatidia with ectopic photoreceptor cells. The positions of these cells suggest they are R3 and R4.

(Li and Carthew, 2005)

Denticle belt fusions in the cuticles of rho^7M43; ru¹ double homozygous embryos are enhanced by P{UAS-Egfr.DN}29-77-1; Scer\GAL4^prd.RG1.

(Urban et al., 2004)

Co-expression of spen^DN.Scer\UAS suppresses the small eye phenotype caused by expression of Egfr^DN.Scer\UAS under the control of Scer\GAL4^GMR.PF.

(Lin et al., 2003)

The loss of midline glial (MG) cells in Egfr^DN.Scer\UAS; Scer\GAL4^sli.PS embryos is suppressed by W^rvX1/W^rvX1. Approximately twice as many MG cells survive as in wild-type (average of 6.1 per segment n=201, compared to average = 2.8 for wild-type).

(Bergmann et al., 2002)

The addition of argos^Δ7 or Dl^X to Egfr^DN.Scer\UAS, Scer\GAL4^twi.PB embryos suppresses their DO2 phenotype. The DA1 phenotype is also suppressed, by homozygous argos^Δ7.

(Carmena et al., 2002)

Border cells in the ovaries of Egfr^DN.Scer\UAS; Pvr^DN.Scer\UAS; Scer\GAL4^slbo.2.6 animals form lack long cellular extensions (20-40 μm) and form only very few intermediate cellular extensions (10-20 μm).

(Fulga and Rorth, 2002)

Egfr^DN.Scer\UAS and Pvr^DN.Scer\UAS, when driven by Scer\GAL4^slbo.2.6, leads to a dramatic effect on border cell migration. 90% of border cell clusters migrate less than half way towards the oocyte. In 5% of egg chambers, border cell clusters are found off the direct track to the oocyte. The combination of Pvf1^EP1624, and Egfr^DN.Scer\UAS (driven by Scer\GAL4^slbo.2.6) leads to strong effect. Border cells are not able to reach the oocyte by stage 10, and they also show a low level of "off track" migration.

(Duchek et al., 2001)

Co-expression of Cbl^Dv.Scer\UAS with Egfr^DN.Scer\UAS under the control of Scer\GAL4^GMR.PF suppresses the phenotypes of both mutants. Retinal development is restored although the eye is smaller than wild-type and ommatidial orientation is irregular. At 29[o]C, veins L2, L3 and L5 are completely rescued, with partial rescue of L4.

(Robertson et al., 2000)

When co-expression of Egfr^DN.Scer\UAS and pan^ΔN.Scer\UAS is driven by Scer\GAL4^T155 the entire tergite is transformed to pleura. When co-expression of Egfr^DN.Scer\UAS and dpp^Scer\UAS.cSa is driven by Scer\GAL4^T155 the tergite is reduced to a very small patch near the dorsal midline and the lateral tergite is completely transformed to pleura. These effects are not due to reduced histoblast proliferation.

(Kopp et al., 1999)

The addition of ovo^Scer\UAS.cPa suppresses the extra naked cuticle seen in embryos expressing Egfr^DN.Scer\UAS driven by either Scer\GAL4^e22c or Scer\GAL4^69B.

(Payre et al., 1999)

When Egfr^DN.Scer\UAS is expressed in combination with dpp^Scer\UAS.cSa under the influence of Scer\GAL4^how-24B, gut morphology is more normal than for dpp^Scer\UAS.cSa, Scer\GAL4^how-24B mutants alone.

(Szuts et al., 1998)

The loss of the DA1 muscle precursor in vn¹⁰⁵⁶⁷ embryos is enhanced if they are also carrying Egfr^DN.Scer\UAS expressed under the control of Scer\GAL4^how-24B.

(Yarnitzky et al., 1998)

Homozygous wg^l-12 larvae carrying Egfr^DN.Scer\UAS which have been shifted to the restrictive temperature after 8 hours of development have denticle belts with almost exclusively large denticles of the row 5 type (although their hooks somewhat resemble the row 1-4 type) and an apparently normal strip of row 6 denticles most posteriorly. Almost all the denticles point posteriorly. Does not alter the denticle phenotype of wg^l-17 larvae when expressed in embryos under the control of Scer\GAL4^arm.PS.

(Szuts et al., 1997)

Xenogenetic Interactions

Statement

Reference

The effect on border cell cluster migration when all border cells co-express Pvr^DN.Scer\UAS and Egfr^DN.Scer\UAS under the control of Scer\GAL4^slbo.2.6 and a single border cell expresses Egfr::Hsap\EGFR^{hE-E.Scer\UAS.T:Avic\GFP} have been studied. In those border cell clusters where the single Egfr::Hsap\EGFR^{hE-E.Scer\UAS.T:Avic\GFP}-expressing cell is located at the front of the cluster, the cluster on average moves forward, when the Egfr::Hsap\EGFR^{hE-E.Scer\UAS.T:Avic\GFP}-expressing cell is in the back, the cluster on average moves relatively more backwards.

(Inaki et al., 2012)

In the absence of illumination, border cells expressing Egfr^DN.Scer\UAS and Pvr^DN.Scer\UAS under the control of Scer\GAL4^slbo.2.6 and carrying Hsap\RAC1^{PA.Q61L.Scer\UAS.T:Disc\RFP-mCherry,T:Zzzz\PT1} fail to move forward. When Hsap\RAC1^{PA.Q61L.Scer\UAS.T:Disc\RFP-mCherry,T:Zzzz\PT1} is photoactivated at the rear of the border cell cluster, rearward movement is seen. When photoactivation is stopped, the border cell cluster stops moving.

Photo-inactivation of Hsap\RAC1^{PA.T17N.Scer\UAS.T:Disc\RFP-mCherry,T:Zzzz\PT1} at the front of border cell clusters expressing Hsap\RAC1^{PA.T17N.Scer\UAS.T:Disc\RFP-mCherry,T:Zzzz\PT1}, Egfr^DN.Scer\UAS and Pvr^DN.Scer\UAS under the control of Scer\GAL4^slbo.2.6 results in local retraction.

(Wang et al., 2010)

Prevention of cell death in Scer\GAL4^btl.PS>Egfr^DN.Scer\UAS embryos, by coexpression of BacA\p35^Scer\UAS.cHa, does not suppress the tracheal branching integrity phenotype.

(Cela and Llimargas, 2006)

The ectopic wing vein phenotype of Scer\GAL4^GMR.PF>Hsap\ATN1^{N917.65Q.Scer\UAS} flies is suppressed when Egfr^DN.Scer\UAS is coexpressed. Wings of flies expressing both transgenes resemble wings that express only Egfr^DN.Scer\UAS.

(Charroux et al., 2006)

Coexpression of BacA\p35^Scer\UAS.cHa and Egfr^DN.Scer\UAS, under the control of Scer\GAL4^en-e16E, in the posterior compartment cells of the embryo leads to a 14% reduction in the size of these cells.

(Parker, 2006)

Complementation and Rescue Data

Comments

Phenotype, when driven by Scer\GAL4^GMR.PF can be rescued by overexpression of wild type Egfr (Egfr^Scer\UAS.cBa).

(Freeman, 1996)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

DER^DN

(Srahna et al., 2006, Zhao et al., 2000)

EGFR^DN

(Wang et al., 2010, Jang et al., 2009)

Egfr^DN.Scer\UAS

Egfr^DN.UAS

Name Synonyms

dominant negative

(Freeman, 1996)

Secondary FlyBase IDs

References (107)

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