FB2024_03 , released June 25, 2024
Allele: Dmel\spenDN.UAS
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General Information
Symbol
Dmel\spenDN.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0151449
Feature type
allele
Associated gene
Dmel\spen
Associated Insertion(s)
Carried in Construct
P{UAS-spen.DN}
Key Links
Transgenic product class
inframe_deletion
(Lin et al., 2003)
Mutagen
Nature of the Allele
Transgenic product class
inframe_deletion
(Lin et al., 2003)
Mutagen
in vitro construct
(Lin et al., 2003)
Progenitor genotype
Carried in construct
P{UAS-spen.DN}
Cytology
Description

UASt regulatory sequences drive expression of the C-terminal 936 amino acids of spen.

(Lin et al., 2003)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
spen
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of spenDN.Scer\UAS under the control of Scer\GAL4sev.PR results in a rough eye phenotype.

      (Jemc and Rebay, 2006)

      Expression of spenDN.Scer\UAS under the control of Scer\GAL4ptc-559.1 in the leg disc results in a complete breakdown of disc morphology at 25oC. At 18-22oC, small leg discs are seen. Expression of spenDN.Scer\UAS under the control of Scer\GAL4da.G32 at 25oC results in embryos with cuticle phenotypes ranging from wild type to moderate reduction of denticle formation. At 29oC, complete disruption of cuticle formation is seen. Expression of spenDN.Scer\UAS under the control of Scer\GAL4da.G32 does not result in ventral cuticle defects, even at 29oC.

      (Lin et al., 2003)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Statement
      Reference

      Scer\GAL4sev.PR, spenDN.UAS has visible phenotype, enhanceable by nitoUAS.Tag:MYC/Scer\GAL4sev.PR

      (Jemc and Rebay, 2006)
      Suppressed by
      Statement
      Reference

      Scer\GAL4sev.PR, spenDN.UAS has visible phenotype, suppressible | partially by nitoRNAi.UAS/Scer\GAL4sev.PR

      (Jemc and Rebay, 2006)
      Suppressor of
      Statement
      Reference

      spenDN.UAS/Scer\GAL4GMR.PF is a suppressor of visible phenotype of armS56F.GMR

      (Lin et al., 2003)

      spenDN.UAS, Scer\GAL4GMR.PF is a suppressor of visible phenotype of EgfrDN.UAS, Scer\GAL4GMR.PF

      (Lin et al., 2003)
      NOT Suppressor of
      Statement
      Reference

      spenDN.UAS/Scer\GAL4GMR.PF is a non-suppressor of visible phenotype of hidGMR.PG

      (Lin et al., 2003)

      spenDN.UAS/Scer\GAL4GMR.PF is a non-suppressor of visible phenotype of rprGMR.PH

      (Lin et al., 2003)
      Phenotype Manifest In
      Enhanced by
      Suppressed by
      Suppressor of
      Statement
      Reference

      spenDN.UAS/Scer\GAL4GMR.PF is a suppressor of eye phenotype of armS56F.GMR

      (Lin et al., 2003)

      spenDN.UAS, Scer\GAL4GMR.PF is a suppressor of eye phenotype of EgfrDN.UAS, Scer\GAL4GMR.PF

      (Lin et al., 2003)
      NOT Suppressor of
      Statement
      Reference

      spenDN.UAS/Scer\GAL4GMR.PF is a non-suppressor of eye phenotype of hidGMR.PG

      (Lin et al., 2003)

      spenDN.UAS/Scer\GAL4GMR.PF is a non-suppressor of eye phenotype of rprGMR.PH

      (Lin et al., 2003)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of nitoScer\UAS.T:Hsap\MYC enhances the rough eye phenotype caused by expression of spenDN.Scer\UAS under the control of Scer\GAL4sev.PR, resulting in complete loss of organisation in the eye and an increase in necroses in the eye.

      Co-expression of nitodsRNA.Scer\UAS partially suppresses the rough eye phenotype caused by expression of spenDN.Scer\UAS under the control of Scer\GAL4sev.PR, with the eyes having fewer missing ommatidia, fewer missing photoreceptor cells in ommatidia lacking the full complement of photoreceptor cells and more normal rhabdomere morphology.

      (Jemc and Rebay, 2006)

      Co-expression of spenDN.Scer\UAS suppresses the reduced eye phenotype caused by expression of armS56F.GMR under the control of Scer\GAL4GMR.PF. Co-expression of spenDN.Scer\UAS suppresses the small eye phenotype caused by expression of EgfrDN.Scer\UAS under the control of Scer\GAL4GMR.PF.

      (Lin et al., 2003)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      spenDN.Scer\UAS
      spenDN.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (2)