FlyBase Allele Report: Dmel\Src64B[UAS.cCa]

General Information

Symbol

Dmel\Src64B^UAS.cCa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Cooper

FlyBase ID

FBal0097107

Feature type

allele

Associated gene

Dmel\Src64B

Associated Insertion(s)

Carried in Construct

P{UAS-Src64B.C}

Also Known As

UAS-Src64B, UAS-Src64

Key Links

Transgenic product class

wild_type

(Wills et al., 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Wills et al., 1999)

Mutagen

in vitro construct

(Wills et al., 1999)

Progenitor genotype

Carried in construct

P{UAS-Src64B.C}

Cytology

Description

Wild-type Src64B is expressed under the control of UAS regulatory sequences.

(Wills et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Src64B

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of cancer

CEA

(Enomoto et al., 2021)

CEA with Ras85D^V12.UAS

(Enomoto et al., 2021)

model of colorectal cancer

CEA

(Cordero et al., 2014)

model of intestinal cancer

CEA

(Nászai et al., 2021)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of cancer

is exacerbated by N^ICN.UAS

(Enomoto et al., 2021)

is ameliorated by Delta^DN.UAS

(Enomoto et al., 2021)

is ameliorated by Delta^RevF10

(Enomoto et al., 2021)

is ameliorated by N^ECN.UAS

(Enomoto et al., 2021)

is ameliorated by N^GD14477

(Enomoto et al., 2021)

is ameliorated by shg^UAS.sgGFP

(Enomoto et al., 2021)

is exacerbated by zfh1^UAS.cPa

(Enomoto et al., 2021)

is ameliorated by Delta^DN.UAS

(Enomoto et al., 2021)

model of colorectal cancer

is ameliorated by Egfr^DN.UAS

(Cordero et al., 2014)

is ameliorated by Ras85D^KK108029

(Cordero et al., 2014)

is ameliorated by Ras85D^GD12553

(Cordero et al., 2014)

is ameliorated by Hsap\MATK^UAS.cVa

(Cordero et al., 2014)

model of intestinal cancer

is ameliorated by Rala^KK108989

(Nászai et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adherens junction | wandering third instar larval stage, with Scer\GAL4^GMR.PF

(Pütz, 2019)

adult mushroom body alpha-lobe, with Scer\GAL4^7B

(Nicolai et al., 2003)

adult mushroom body alpha-lobe, with Scer\GAL4^ey-OK107

(Nicolai et al., 2003)

adult mushroom body beta-lobe, with Scer\GAL4^7B

(Nicolai et al., 2003)

adult mushroom body beta-lobe, with Scer\GAL4^ey-OK107

(Nicolai et al., 2003)

border follicle cell, with Scer\GAL4^slbo.2.6

(Rorth et al., 2000)

centripetal follicle cell, with Scer\GAL4^slbo.2.6

(Rorth et al., 2000)

eye disc | wandering third instar larval stage, with Scer\GAL4^GMR.PF

(Pütz, 2019)

intestinal stem cell of posterior adult midgut epithelium, with Scer\GAL4^NP5130

(Cordero et al., 2014)

Kenyon cell, with Scer\GAL4^7B

(Nicolai et al., 2003)

Kenyon cell, with Scer\GAL4^ey-OK107

(Nicolai et al., 2003)

mushroom body & axon, with Scer\GAL4^7B

(Nicolai et al., 2003)

mushroom body & axon, with Scer\GAL4^ey-OK107

(Nicolai et al., 2003)

photoreceptor R3 precursor cell | wandering third instar larval stage, with Scer\GAL4^GMR.PF

(Pütz, 2019)

photoreceptor R4 precursor cell | wandering third instar larval stage, with Scer\GAL4^GMR.PF

(Pütz, 2019)

Detailed Description

Statement

Reference

Expressing Src64B^UAS.cCa under the control of Scer\GAL4^GMR.PF leads to massive defects in eye development when flies are raised at 25[o]C, but only mild defects at 18[o]C. At 18[o]C a significant proportion of CadN-immunopositive adherens junctions between photoreceptor precursor cells R3 and R4 in row 5 ommatidia of wandering third instar larvae are shorter compared to controls.

(Pütz, 2019)

Scer\GAL4^esg-NP5130-mediated expression of Src64B^Scer\UAS.cCa (restricted to adult stages by using P{tubP-GAL80^ts}) results in substantial intestinal stem cell hyperproliferation and hyperplasia of the adult intestine.

(Cordero et al., 2014)

When expression is driven by Scer\GAL4^7B, Src64B^Scer\UAS.cCa shows a mushroom body Class III phenotype; axonal growth and guidance defects. The beta lobes are abortive and the alpha lobes enlarged. Phenotype is similar though more dramatic - all axons stop at the peduncle - when Src64B^Scer\UAS.cCa is driven by Scer\GAL4^OK107.

(Nicolai et al., 2003)

Expression of Src64B^Scer\UAS.cCa under the control of Scer\GAL4^slbo.2.6 impairs border cell and centripetal cell migration.

(Rorth et al., 2000)

ISNb bypass phenotypes are not produced when Src64B^Scer\UAS.cCa is expressed under the control of Scer\GAL4^elav.PLu.

(Wills et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement