FlyBase Allele Report: Dmel\hppy[RB.UAS]

General Information

Symbol

Dmel\hppy^RB.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0230292

Feature type

allele

Associated gene

Dmel\hppy

Associated Insertion(s)

Carried in Construct

P{UAS-hppy.RB}

Key Links

Transgenic product class

cDNA

(Corl et al., 2009)

wild_type

(Corl et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Corl et al., 2009)

wild_type

(Corl et al., 2009)

Mutagen

in vitro construct

(Corl et al., 2009)

Progenitor genotype

Carried in construct

P{UAS-hppy.RB}

Cytology

Description

UASt regulatory sequences drive expression of the hppy-RB isoform (from cDNA clone RH10407).

(Corl et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

hppy

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of alcohol use disorder

CEA

(Corl et al., 2009)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

chemical sensitive, with Scer\GAL4^elav-C155

(Corl et al., 2009)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of hppy^RB.Scer\UAS under the control of Scer\GAL4^elav-C155 results in flies with an increased sensitivity to ethanol-induced sedation compared to control flies.

Expression of hppy^RB.Scer\UAS under the control of Scer\GAL4^GMR.PF has little, if any, effect on eye morphology.

(Corl et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

hppy^RB.UAS, Scer\GAL4^GMR.PF is an enhancer of visible phenotype of Egfr^GD1654, Scer\GAL4^GMR.PF

(Corl et al., 2009)

hppy^RB.UAS, Scer\GAL4^GMR.PF is an enhancer of partially lethal - majority die phenotype of Egfr^DN.UAS, Scer\GAL4^GMR.PF

(Corl et al., 2009)

hppy^RB.UAS/Scer\GAL4^GMR.PF is an enhancer of partially lethal - majority die phenotype of Scer\GAL4^GMR.PF, aop^ACT.UAS

(Corl et al., 2009)

Suppressor of

Statement

Reference

hppy^RB.UAS, Scer\GAL4^GMR.PF is a suppressor of visible phenotype of Egfr^UAS.cBa, Scer\GAL4^GMR.PF

(Corl et al., 2009)

NOT Suppressor of

Statement

Reference

hppy^RB.UAS/Scer\GAL4^GMR.PF is a non-suppressor of visible phenotype of Scer\GAL4^GMR.PF, rl^Sem.C.UAS

(Corl et al., 2009)

Other

Statement

Reference

Scer\GAL4^GMR.PF, aop^UAS.cRa, hppy^RB.UAS has visible phenotype

(Corl et al., 2009)

Phenotype Manifest In

Enhancer of

Statement

Reference

hppy^RB.UAS, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Egfr^GD1654, Scer\GAL4^GMR.PF

(Corl et al., 2009)

Suppressor of

Statement

Reference

hppy^RB.UAS, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Egfr^UAS.cBa, Scer\GAL4^GMR.PF

(Corl et al., 2009)

NOT Suppressor of

Statement

Reference

hppy^RB.UAS/Scer\GAL4^GMR.PF is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PF, rl^Sem.C.UAS

(Corl et al., 2009)

Other

Statement

Reference

Scer\GAL4^GMR.PF, aop^UAS.cRa, hppy^RB.UAS has eye phenotype

(Corl et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of hppy^RB.Scer\UAS has no effect on the rough eye phenotype caused by expression of rl^{Sem.C.Scer\UAS} under the control of Scer\GAL4^GMR.PF.

Co-expression of hppy^RB.Scer\UAS suppresses the rough eye and blistering phenotypes caused by expression of Egfr^Scer\UAS.cBa under the control of Scer\GAL4^GMR.PF.

Co-expression of both aop^Scer\UAS.cRa and hppy^RB.Scer\UAS under the control of Scer\GAL4^GMR.PF results in a severe rough and "glossy" eye phenotype.

The mild rough eye phenotype caused by expression of Egfr^GD1654 under the control of Scer\GAL4^GMR.PF is potently enhanced by co-expression of hppy^RB.Scer\UAS.

(Corl et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

hppy^RB.UAS/Scer\GAL4^hppy-17-51 rescues hppy^17-51

(Corl et al., 2009)

Scer\GAL4^elav-C155/hppy^RB.UAS rescues hppy^KG05537

(Corl et al., 2009)

Comments

The phenotype of increased resistance to ethanol-induced sedation that is seen in hppy^17-51 flies is rescued by expression of hppy^RB.Scer\UAS under the control of Scer\GAL4^hppy-17-51, such that the rescued flies have normal resistance to ethanol.

The phenotype of increased resistance to ethanol-induced sedation that is seen in hppy^KG05537 flies is rescued by expression of hppy^RB.Scer\UAS under the control of Scer\GAL4^elav-C155, such that the rescued flies have normal resistance to ethanol.

(Corl et al., 2009)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

hppy^RB.Scer\UAS

hppy^RB.UAS

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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