FlyBase Allele Report: Dmel\rho-5[KO1]

General Information

Symbol

Dmel\rho-5^KO1

Species

D. melanogaster

Name

FlyBase ID

FBal0265931

Feature type

allele

Associated gene

Dmel\rho-5

Associated Insertion(s)

TI{TI}rho-5^KO1

Carried in Construct

Also Known As

iRhom^KO1

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Zettl et al., 2011)

Mutagen

ends-out gene targeting

(Zettl et al., 2011)

FLPase

(Zettl et al., 2011)

I-SceI

(Zettl et al., 2011)

Progenitor genotype

Associated Insertion(s)

TI{TI}rho-5^KO1

Cytology

Description

The rho-5 coding region has been replaced by a w^+mW.hs marker.

(Zettl et al., 2011)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Huntington's disease

modeled by Hsap\HTT^Q128.UAS

(Lee et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal sleep (with Df(2L)J17)

(Zettl et al., 2011)

abnormal sleep | recessive

(Zettl et al., 2011)

viable

(Zettl et al., 2011)

Phenotype Manifest In

Detailed Description

Statement

Reference

Homozygous rho-5^KO1 flies do not exhibit any discernible developmental defects. However, a severe decrease in daytime activity is seen in adult flies compared to wild type or heterozygotes. rho-5^KO1 mutant flies sometimes do not move for periods of 1 hr, which is highly unusual, and have a reduced number of active periods over a given time. However, when the flies are active they move as much if not more than control flies and have a similar walking speed. The number of periods of sleep (defined as a period of activity of more than 5 minutes) is increased in rho-5^KO1 mutants.

rho-5^KO1/Df(2L)J17 flies do not exhibit any discernible developmental defects. However, a severe decrease in daytime activity is seen in adult flies compared to wild type or heterozygotes.

(Zettl et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

rho-5[+]/rho-5^KO1, sty^S73 has visible | dominant phenotype, enhanceable by aos[+]/aos^Δ7

(Zettl et al., 2011)

Suppressed by

Statement

Reference

rho-5^KO1 has abnormal sleep | recessive phenotype, suppressible | partially by Scer\GAL4^elav-C155/Egfr^GD1654

(Zettl et al., 2011)

rho-5^KO1 has abnormal sleep | recessive phenotype, suppressible | partially by Scer\GAL4^elav-C155/spi^GD1779

(Zettl et al., 2011)

Enhancer of

Statement

Reference

rho-5[+]/rho-5^KO1 is an enhancer of visible phenotype of Hsap\HTT^Q128.UAS, Scer\GAL4^GMR.PU

(Lee et al., 2015)

rho-5[+]/rho-5^KO1 is an enhancer of visible | dominant phenotype of sty^S73

(Zettl et al., 2011)

rho-5[+]/rho-5^KO1 is an enhancer of visible | dominant phenotype of aos[+]/aos^Δ7, sty^S73

(Zettl et al., 2011)

Suppressor of

Statement

Reference

rho-5^KO1 is a suppressor | partially of visible phenotype of Scer\GAL4^GMR.PF, aos^UAS.cHa

(Zettl et al., 2011)

rho-5^KO1 is a suppressor | partially of visible phenotype of Scer\GAL4^GMR.PF, sty^UAS.N.Tag:HA

(Zettl et al., 2011)

rho-5^KO1 is a suppressor | partially of visible phenotype of Egfr^DN.UAS, Scer\GAL4^GMR.PF

(Zettl et al., 2011)

Phenotype Manifest In

Enhanced by

Statement

Reference

rho-5[+]/rho-5^KO1, sty^S73 has wing vein phenotype, enhanceable by aos[+]/aos^Δ7

(Zettl et al., 2011)

Enhancer of

Statement

Reference

rho-5[+]/rho-5^KO1 is an enhancer of eye phenotype of Hsap\HTT^Q128.UAS, Scer\GAL4^GMR.PU

(Lee et al., 2015)

rho-5[+]/rho-5^KO1 is an enhancer of ommatidium phenotype of Hsap\HTT^Q128.UAS, Scer\GAL4^GMR.PU

(Lee et al., 2015)

rho-5[+]/rho-5^KO1 is an enhancer of wing vein phenotype of sty^S73

(Zettl et al., 2011)

rho-5[+]/rho-5^KO1 is an enhancer of wing vein phenotype of aos[+]/aos^Δ7, sty^S73

(Zettl et al., 2011)

Suppressor of

Statement

Reference

rho-5^KO1 is a suppressor | partially of eye phenotype of Scer\GAL4^GMR.PF, aos^UAS.cHa

(Zettl et al., 2011)

rho-5^KO1 is a suppressor | partially of eye phenotype of Scer\GAL4^GMR.PF, sty^UAS.N.Tag:HA

(Zettl et al., 2011)

rho-5^KO1 is a suppressor | partially of eye phenotype of Egfr^DN.UAS, Scer\GAL4^GMR.PF

(Zettl et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

rho-5^KO1/+ strongly enhances the rough eye phenotype seen in rho^hs.sev.

A homozygous rho-5^KO1 background suppresses the rough eye phenotype seen when aos^Scer\UAS.cHa is expressed under the control of Scer\GAL4^GMR.PF.

One copy of rho-5^KO1 enhances the extra wing vein phenotype seen in sty^S73 heterozygotes at 29[o]C.

One copy of aos^Δ7 enhances the extra vein phenotype seen in rho-5^KO1 sty^S73 double heterozygotes at 29[o]C.

Expression of Egfr^GD1654 under the control of Scer\GAL4^elav-C155 significantly suppresses the sleep defects seen in homozygous rho-5^KO1 mutants at 29[o]C.

Expression of spi^GD1779 under the control of Scer\GAL4^elav-C155 significantly suppresses the sleep defects seen in homozygous rho-5^KO1 mutants at 29[o]C.

(Zettl et al., 2011)

Xenogenetic Interactions

Statement

Reference

rho-5^KO1/+ enhances the rough eye phenotype and reduced numbers of ommatidia seen in flies with Hsap\HTT^{Q128.Scer\UAS} driven by Scer\GAL4^GMR.PU.

(Lee et al., 2015)

Complementation and Rescue Data

Rescued by

rho-5^KO1 is rescued by rho-5^UAS.cZa/Scer\GAL4^elav-C155

(Zettl et al., 2011)

Not rescued by

rho-5^KO1 is not rescued by rho-5^UAS.cZa/Scer\GAL4^how-24B

(Zettl et al., 2011)

Comments

Expression of rho-5^Scer\UAS.cZa under the control of Scer\GAL4^elav-C155 fully rescues the reduction in daytime activity seen in rho-5^KO1 mutants. Expression of rho-5^Scer\UAS.cZa under the control of the muscle driver Scer\GAL4^how-24B is unable to rescue the phenotype.

(Zettl et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

iRhom^KO1

(Lee et al., 2015, Zettl et al., 2011)

rho-5^KO1

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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