FlyBase Allele Report: Dmel\sty[UAS.N.Tag:HA]

General Information

Symbol

Dmel\sty^UAS.N.Tag:HA

Species

D. melanogaster

Name

FlyBase ID

FBal0096669

Feature type

allele

Associated gene

Dmel\sty

Associated Insertion(s)

Carried in Construct

P{UAS-sty.N.T:HA1}

Key Links

Transgenic product class

wild_type

(Casci et al., 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Casci et al., 1999)

Mutagen

in vitro construct

(Casci et al., 1999)

Progenitor genotype

Carried in construct

P{UAS-sty.N.T:HA1}

Cytology

Description

UASt regulatory sequences drive expression of sty, tagged between residues 81 and 82 with three copies of Tag:HA.

(Casci et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

sty

Tagged with

Tag:HA

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^Bx-MS1096

(Casci et al., 1999)

visible, with Scer\GAL4^GMR.PF

(Zettl et al., 2011)

visible, with Scer\GAL4^hs.2sev

(Casci et al., 1999)

Phenotype Manifest In

cone cell, with Scer\GAL4^hs.2sev

(Casci et al., 1999)

eye, with Scer\GAL4^GMR.PF

(Zettl et al., 2011)

eye, with Scer\GAL4^hs.2sev

(Casci et al., 1999)

eye photoreceptor cell, with Scer\GAL4^hs.2sev

(Casci et al., 1999)

five-cell ommatidial precursor cluster, with Scer\GAL4^GMR.PF

(Casci et al., 1999)

ommatidial precursor cluster, with Scer\GAL4^GMR.PF

(Casci et al., 1999)

pigment cell, with Scer\GAL4^hs.2sev

(Casci et al., 1999)

wing, with Scer\GAL4^Bx-MS1096

(Casci et al., 1999)

wing vein, with Scer\GAL4^Bx-MS1096

(Casci et al., 1999)

Detailed Description

Statement

Reference

Expression of sty^{Scer\UAS.N.T:Ivir\HA1} under the control of Scer\GAL4^GMR.PF produces a rough eye phenotype.

(Zettl et al., 2011)

Flies expressing sty^{Scer\UAS.N.T:Ivir\HA1} under the control of Scer\GAL4^hs.2sev have extremely rough eyes. There is a reduction in the number of photoreceptor, cone and pigment cells. Initial recruitment of photoreceptor cells is normal in eye discs in which sty^{Scer\UAS.N.T:Ivir\HA1} is expressed under the control of Scer\GAL4^GMR.PF, but never proceeds beyond the three- or five-cell stage. Flies expressing sty^{Scer\UAS.N.T:Ivir\HA1} under the control of Scer\GAL4^Bx-MS1096 show a reduction in wing size of about 50% and a substantial loss of wing veins.

(Casci et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, sty^UAS.N.Tag:HA has visible phenotype, suppressible | partially by rho-5^KO1

(Zettl et al., 2011)

Scer\GAL4^GMR.PF, sty^UAS.N.Tag:HA has visible phenotype, suppressible | partially by sip3^GD3145/Scer\GAL4^GMR.PF

(Zettl et al., 2011)

Scer\GAL4^GMR.PF, sty^UAS.N.Tag:HA has visible phenotype, suppressible | partially by Edem2^RNAi.UAS, Scer\GAL4^GMR.PF

(Zettl et al., 2011)

Suppressor of

Statement

Reference

sty^UAS.N.Tag:HA, Scer\GAL4^hs.2sev is a suppressor of visible phenotype of Egfr::tor^{t4021E.hs.sev}, Scer\GAL4^hs.2sev

(Casci et al., 1999)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, sty^UAS.N.Tag:HA has eye phenotype, suppressible | partially by rho-5^KO1

(Zettl et al., 2011)

Scer\GAL4^GMR.PF, sty^UAS.N.Tag:HA has eye phenotype, suppressible | partially by sip3^GD3145/Scer\GAL4^GMR.PF

(Zettl et al., 2011)

Scer\GAL4^GMR.PF, sty^UAS.N.Tag:HA has eye phenotype, suppressible | partially by Edem2^RNAi.UAS, Scer\GAL4^GMR.PF

(Zettl et al., 2011)

Suppressor of

Statement

Reference

sty^UAS.N.Tag:HA, Scer\GAL4^hs.2sev is a suppressor of eye phenotype of Egfr::tor^{t4021E.hs.sev}, Scer\GAL4^hs.2sev

(Casci et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

A homozygous rho-5^KO1 background suppresses the rough eye phenotype seen when sty^{Scer\UAS.N.T:Ivir\HA1} is expressed under the control of Scer\GAL4^GMR.PF.

Expression of sip3^GD3145 suppresses the rough eye phenotype seen when sty^{Scer\UAS.N.T:Ivir\HA1} is expressed under the control of Scer\GAL4^GMR.PF.

Expression of Edem2^{dsRNA.Scer\UAS} suppresses the rough eye phenotype seen when sty^{Scer\UAS.N.T:Ivir\HA1} is expressed under the control of Scer\GAL4^GMR.PF.

(Zettl et al., 2011)

The eye phenotype of flies expressing Egfr::tor^{t4021E.hs.sev} is suppressed by sty^{Scer\UAS.N.T:Ivir\HA1} expressed under the control of Scer\GAL4^hs.2sev.

(Casci et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

sty^{Scer\UAS.N.T:Ivir\HA1}

sty^UAS.N.Tag:HA

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

Open Close