FlyBase Allele Report: Dmel\Cbl[Dv.UAS]

General Information

Symbol

Dmel\Cbl^Dv.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0243527

Feature type

allele

Associated gene

Dmel\Cbl

Associated Insertion(s)

Carried in Construct

P{UAS-Cbl.Dv}

Key Links

Transgenic product class

inframe_deletion

(Robertson et al., 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Robertson et al., 2000)

Mutagen

in vitro construct

(Robertson et al., 2000)

Progenitor genotype

Carried in construct

P{UAS-Cbl.Dv}

Cytology

Description

UASt regulatory sequences drive expression of a 1.5kb fragment of Cbl coding amino acids 1-345 (truncated at conserved residue 306)

(Robertson et al., 2000)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Cbl

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

eye, with Cbl¹, Scer\GAL4^GMR.PF

(Robertson et al., 2000)

eye, with Scer\GAL4^GMR.PF

(Robertson et al., 2000)

eye (with Cbl¹), with Scer\GAL4^GMR.PF

(Robertson et al., 2000)

ommatidium, with Cbl¹, Scer\GAL4^GMR.PF

(Robertson et al., 2000)

ommatidium (with Cbl¹), with Scer\GAL4^GMR.PF

(Robertson et al., 2000)

photoreceptor cell R7, with Scer\GAL4^GMR.PF

(Robertson et al., 2000)

photoreceptor neuron | ectopic, with Cbl¹, Scer\GAL4^GMR.PF

(Robertson et al., 2000)

photoreceptor neuron | ectopic (with Cbl¹), with Scer\GAL4^GMR.PF

(Robertson et al., 2000)

wing vein | ectopic, with Scer\GAL4^GMR.PF

(Robertson et al., 2000)

Detailed Description

Statement

Reference

Expression of Cbl^Dv.Scer\UAS in the eye under the control of Scer\GAL4^GMR.PF generates rough eyes, with some ectopic R7-like photoreceptors, but does not generate tumours.

Expression of Cbl^Dv.Scer\UAS under the control of Scer\GAL4^en-e16E causes hyperplasia of the humeral region in 5% of the progeny.

Expression of Cbl^Dv.Scer\UAS under the control of Scer\GAL4^GMR.PF causes some ectopic wing vein formation.

Flies heterozygous for both Cbl¹ and Scer\GAL4^GMR.PF;Cbl^Dv.Scer\UAS exhibit a significantly enhanced Scer\GAL4^GMR.PF;Cbl^Dv.Scer\UAS phenotype, with pronounced disruption of the ommatidial array and an increase in the number of ommatidia with ectopic photoreceptors.

(Robertson et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Cbl^Dv.UAS, Ras85D^V12.sev, Scer\GAL4^en-e16E has hyperplasia phenotype

(Robertson et al., 2000)

Cbl^Dv.UAS, Scer\GAL4^en-e16E, sev^S11.Tag:MYC has hyperplasia phenotype

(Robertson et al., 2000)

Phenotype Manifest In

Enhanced by

Statement

Reference

Cbl^Dv.UAS, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by Egfr^UAS.cBa, Scer\GAL4^GMR.PF

(Robertson et al., 2000)

Cbl^Dv.UAS, Scer\GAL4^GMR.PF has photoreceptor cell R7 phenotype, enhanceable by Egfr^UAS.cBa, Scer\GAL4^GMR.PF

(Robertson et al., 2000)

Cbl^Dv.UAS, Scer\GAL4^GMR.PF has wing vein | ectopic phenotype, enhanceable by Dap160^EP2543, Scer\GAL4^GMR.PF

(Robertson et al., 2000)

Cbl^Dv.UAS, Scer\GAL4^GMR.PF has wing vein | ectopic phenotype, enhanceable by hyd¹⁵

(Robertson et al., 2000)

Cbl^Dv.UAS, Scer\GAL4^GMR.PF has wing vein phenotype, enhanceable by Egfr^UAS.cBa, Scer\GAL4^GMR.PF

(Robertson et al., 2000)

Cbl^Dv.UAS, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by aos^Δ7

(Robertson et al., 2000)

Cbl^Dv.UAS, Scer\GAL4^GMR.PF has photoreceptor cell R7 phenotype, enhanceable by aos^Δ7

(Robertson et al., 2000)

Cbl^Dv.UAS, Scer\GAL4^GMR.PF has wing vein | ectopic phenotype, enhanceable by aos^Δ7

(Robertson et al., 2000)

Cbl^Dv.UAS, Scer\GAL4^GMR.PF has wing vein | ectopic phenotype, enhanceable by shi¹

(Robertson et al., 2000)

Cbl^Dv.UAS, Scer\GAL4^GMR.PF has wing vein | ectopic phenotype, enhanceable by shi²

(Robertson et al., 2000)

Cbl^Dv.UAS, Scer\GAL4^GMR.PF has wing vein | ectopic phenotype, enhanceable by shi⁴

(Robertson et al., 2000)

Cbl^Dv.UAS, Scer\GAL4^GMR.PF has wing vein | ectopic phenotype, enhanceable by Dap160^EP2468, Scer\GAL4^GMR.PF

(Robertson et al., 2000)

NOT Enhanced by

Statement

Reference

Cbl^Dv.UAS, Scer\GAL4^GMR.PF has wing vein | ectopic phenotype, non-enhanceable by lwr⁰²⁸⁵⁸

(Robertson et al., 2000)

Cbl^Dv.UAS, Scer\GAL4^GMR.PF has wing vein | ectopic phenotype, non-enhanceable by lwr⁰⁵⁴⁸⁶

(Robertson et al., 2000)

Suppressed by

Statement

Reference

Cbl^Dv.UAS, Scer\GAL4^GMR.PF has eye phenotype, suppressible by Egfr^DN.UAS, Scer\GAL4^GMR.PF

(Robertson et al., 2000)

Cbl^Dv.UAS, Scer\GAL4^GMR.PF has photoreceptor cell R7 phenotype, suppressible by Egfr^DN.UAS, Scer\GAL4^GMR.PF

(Robertson et al., 2000)

Cbl^Dv.UAS, Scer\GAL4^GMR.PF has wing vein phenotype, suppressible by Egfr^DN.UAS, Scer\GAL4^GMR.PF

(Robertson et al., 2000)

NOT suppressed by

Statement

Reference

Cbl^Dv.UAS, Scer\GAL4^GMR.PF has wing vein | ectopic phenotype, non-suppressible by lwr⁰²⁸⁵⁸

(Robertson et al., 2000)

Cbl^Dv.UAS, Scer\GAL4^GMR.PF has wing vein | ectopic phenotype, non-suppressible by lwr⁰⁵⁴⁸⁶

(Robertson et al., 2000)

Suppressor of

Statement

Reference

Cbl^Dv.UAS, Scer\GAL4^GMR.PF is a suppressor of retina phenotype of Egfr^DN.UAS, Scer\GAL4^GMR.PF

(Robertson et al., 2000)

Cbl^Dv.UAS, Scer\GAL4^GMR.PF is a suppressor of wing vein L2 phenotype of Egfr^DN.UAS, Scer\GAL4^GMR.PF

(Robertson et al., 2000)

Cbl^Dv.UAS, Scer\GAL4^GMR.PF is a suppressor of wing vein L3 phenotype of Egfr^DN.UAS, Scer\GAL4^GMR.PF

(Robertson et al., 2000)

Cbl^Dv.UAS, Scer\GAL4^GMR.PF is a suppressor of wing vein L4 phenotype of Egfr^DN.UAS, Scer\GAL4^GMR.PF

(Robertson et al., 2000)

Cbl^Dv.UAS, Scer\GAL4^GMR.PF is a suppressor of wing vein L5 phenotype of Egfr^DN.UAS, Scer\GAL4^GMR.PF

(Robertson et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Cbl^Dv.Scer\UAS in the eye under the control of Scer\GAL4^GMR.PF in combination with Ras85D^V12.sev at 25[o]C or sev^{S11.T:Hsap\MYC} at 29[o]C produces melanotic tumours in the eyes in approximately 30 and 40% of progeny, respectively.

Expression of both Cbl^Dv.Scer\UAS and Egfr^Scer\UAS.cBa under the control of Scer\GAL4^GMR.PF significantly enhances both the rough eye and extent of ectopic wing veins.

Co-expression of Cbl^Dv.Scer\UAS with Egfr^DN.Scer\UAS under the control of Scer\GAL4^GMR.PF suppresses the phenotypes of both mutants. Retinal development is restored although the eye is smaller than wild-type and ommatidial orientation is irregular. At 29[o]C, veins L2, L3 and L5 are completely rescued, with partial rescue of L4.

A argos^Δ7 background enhances the eye and wing vein phenotype of Scer\GAL4^GMR.PF Cbl^Dv.Scer\UAS mutants.

Thirty-seven percent of shi¹; Scer\GAL4^GMR.PF Cbl^Dv.Scer\UAS wings exhibit an ectopic vein phenotype, moreso than in Scer\GAL4^GMR.PF Cbl^Dv.Scer\UAS mutants.

shi²; Scer\GAL4^GMR.PF Cbl^Dv.Scer\UAS wings exhibit an ectopic vein phenotype, moreso than in Scer\GAL4^GMR.PF Cbl^Dv.Scer\UAS mutants.

shi⁴; Scer\GAL4^GMR.PF Cbl^Dv.Scer\UAS wings exhibit an ectopic vein phenotype, moreso than in Scer\GAL4^GMR.PF Cbl^Dv.Scer\UAS mutants.

Co-expression of Cbl^Dv.Scer\UAS with Dap160^EP2468, both under the control of Scer\GAL4^GMR.PF results in an increase in ectopic wing veins compared to expression of Cbl^Dv.Scer\UAS alone.

Co-expression of Cbl^Dv.Scer\UAS with Dap160^EP2543, both under the control of Scer\GAL4^GMR.PF results in an increase in ectopic wing veins compared to expression of Cbl^Dv.Scer\UAS alone.

The extent of ectopic wing vein formation found in Scer\GAL4^GMR.PF Cbl^Dv.Scer\UAS flies is enhanced in a hyd¹⁵ mutant background but does not affect the eye roughening phenotype.

A lwr⁰²⁸⁵⁸ mutant background does not affect the extent of ectopic wing veins or eye roughening seen in Scer\GAL4^GMR.PF Cbl^Dv.Scer\UAS mutants.

A lwr⁰⁵⁴⁸⁶ mutant background does not affect the extent of ectopic wing veins or eye roughening seen in Scer\GAL4^GMR.PF Cbl^Dv.Scer\UAS mutants.

(Robertson et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

(Robertson et al., 2000)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Cbl^Dv.Scer\UAS

Cbl^Dv.UAS

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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