FB2024_04 , released June 25, 2024
Allele: Scer\GAL4Dll-md23
Open Close
General Information
Symbol
Scer\GAL4Dll-md23
Species
S. cerevisiae
Name
FlyBase ID
FBal0052388
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
Dll-GAL4, DllGal4, dllmd23-Gal4, Dllmd23, Dll-GAL4md23, Dll-GAL4 (MD23), dll.Gal4, P[Dll-Gal4], dll, MD23
Allele class
Nature of the Allele
Allele class
Progenitor genotype
Associated Insertion(s)
Cytology
Description
Allele components
Component
Use(s)
Inserted element
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DNA sequence
Protein sequence
 
Expression Data
Reporter Expression
No Assay Recorded
Stage
Tissue/Position (including subcellular localization)
Reference
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference

Drives expression in presumptive distal structures in imaginal discs, including the presumptive tarsal segments of leg discs, the presumptive wing margin, the presumptive second and third segments and arista of the antennal disc, and distal structures of male and female genital discs.

Comparing ScerGAL4Dll-md23 with anti-Dll staining shows that the Gal4 driver faithfully recapitulates Dll expression with two exceptions: (1) the embryonic ventral nerve cord glia that express Dll lack ScerGAL4Dll-md23 expression; and (2) several cells in thebrain that express Dll, also lack ScerGAL4Dll-md23 expression.

Dll reporter is expressed in a small population of neurons located at the most proximal part of the outer optic lobe (neurons generated by the oldest neuroblasts to have emerged from the neuroepithelium) but not in the neuroblasts themselves. Expression persists in pupae.

ScerGAL4Dll-md23 drives expression in 20% of cells in the medulla cortex and medulla rim. Neurons in the medulla rim have processes in the lamina. Cells labeled with ScerGAL4Dll-md23 do not overlap with cells expressing EcollacZap-rK568 or ScerGAL4ey-OK107.

Reporter expression was used to trace the movement of wing primordial cells in the embryo. In embryonic stages 12-14, expressing cell clusters in each thoracic segment extend in the dorsoventral direction. By stage 15, wing and haltere cells are well separated from leg primordia. A dorsal cluster of expressing cells is also observed in the prothoracic segment.

Larval expression is observed in the genital and anal primordia of the male and female genital discs. Adult expression is observed in the vaginal plates in females, in the claspers in males, and in the anal plates of both sexes.

Assayed in adult cuticle using y+ marker. Expressed in distal portions of leg, wing, and antenna; expression in femur relatively weak.

Expression in distal portions of leg and antenna; along dorsal/ventral boundary of wing. Observed as adult y+ phenotype; expression assumed to be earlier.

 
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

adult apodeme & leg, with aosUAS.cHa

antennal segment 3 & bract | ectopic, with ftzLRAAA.UAS

antennal segment 3 & bract | ectopic, with Tcas\ftzFNWS.UAS

antennal segment 3 & macrochaeta | ectopic, with danRNAi.UAS.cSa

antennal segment 3 & macrochaeta | ectopic, with danrRNAi.UAS

leg & joint, with fngUAS.cKa

mesothoracic tarsal segment 1 & bract, with aosUAS.cHa

mesothoracic tarsal segment 1 & bract | dorsal, with aosUAS.cHa

taste bristle & leg | ectopic, with aosUAS.cHa

taste bristle & leg | ectopic, with EgfrUAS.cBa

taste bristle & leg | ectopic, with spis.UAS

wing & microchaeta | distal, with fzUAS.cAa

wing sensillum & sensillum campaniformium | ectopic, with vgUAS.cZa

Detailed Description
Statement
Reference
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
Enhancer of
NOT Enhancer of
Suppressor of
Statement
Reference
NOT Suppressor of
Phenotype Manifest In
Enhanced by
Suppressed by
Enhancer of
NOT Enhancer of
Suppressor of
Statement
Reference

fzUAS.cAa/Scer\GAL4Dll-md23 is a suppressor of wing hair & 1st posterior cell phenotype of in1

NOT Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (3)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Scer\GAL4Dll-md23
Name Synonyms
Secondary FlyBase IDs
    References (127)