FlyBase Allele Report: Dmel\y[UAS.cCa]

General Information

Symbol

Dmel\y^UAS.cCa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Calleja

FlyBase ID

FBal0050836

Feature type

allele

Associated gene

Dmel\y

Associated Insertion(s)

Carried in Construct

P{UAS-y.C}

Also Known As

UAS-y, UAS-y⁺, UAS-yellow

Key Links

Transgenic product class

cDNA

(Calleja et al., 1996)

wild_type

(Calleja et al., 1996)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Calleja et al., 1996)

wild_type

(Calleja et al., 1996)

Mutagen

in vitro construct

(Gorfinkiel et al., 1997, Calleja, 1996.10.16, Calleja et al., 1996, Heitzler et al., 1996, Lecuit et al., 1996)

Progenitor genotype

Carried in construct

P{UAS-y.C}

Cytology

Description

UASt regulatory sequences drive expression of a full length y cDNA.

(Calleja et al., 1996)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal body color, with Scer\GAL4^en-e16E

(Riedel et al., 2011)

abnormal body color, with Scer\GAL4^pnr-MD237

(Wittkopp et al., 2002)

Phenotype Manifest In

adult cuticle, with Scer\GAL4^hs.PB

(Calleja et al., 1996)

adult cuticle, with Scer\GAL4^pnr-MD237

(Calleja et al., 1996)

analia, with Scer\GAL4^cad-em459

(Calleja et al., 1996)

antennal segment, with Scer\GAL4^Dll-md23

(Calleja et al., 1996)

metatarsus, with Scer\GAL4^Dll-md23

(Calleja et al., 1996)

pretarsus, with Scer\GAL4^Dll-md23

(Calleja et al., 1996)

tarsal segment 2, with Scer\GAL4^Dll-md23

(Calleja et al., 1996)

tarsal segment 3, with Scer\GAL4^Dll-md23

(Calleja et al., 1996)

tarsal segment 4, with Scer\GAL4^Dll-md23

(Calleja et al., 1996)

tibia, with Scer\GAL4^Dll-md23

(Calleja et al., 1996)

wing, with Scer\GAL4^Dll-md23

(Calleja et al., 1996)

wing blade posterior compartment, with Scer\GAL4^en-e16E

(Riedel et al., 2011)

Detailed Description

Statement

Reference

The posterior compartment of the pupal wing undergoes excess pigmentation (showing black rather than brown pigmentation) in the presence of dopamine in an in vitro melanisation assay in animals expressing y^Scer\UAS.cCa under the control of Scer\GAL4^en-e16E.

(Riedel et al., 2011)

When y^Scer\UAS.cCa is driven by Scer\GAL4^pnr-MD237, pigmentation in the thorax is slightly darkened compared to wild-type flies. The colour intensity and the anterior posterior width of the abdominal pigmentation is mildly increased. The lateral width of the pigment along the dorsal midline also increases. However in many cells, such as those anterior to the pigment stripe, no black pigemtn is induced.

(Wittkopp et al., 2002)

When expressed under the influence of Scer\GAL4^hs.PB, heat shocks given at or after the second half of the third larval period are able to produce y⁺ rescue. When expressed via Scer\GAL4^Dll-md23 the tibia, basitarsus and tarsal segments and a small region at the trochanter-femur joint are pigmented, as well as along the dorsoventral boundary in the wing and in the distal antennal segments. When expressed via Scer\GAL4^cad-em459 the analia are pigmented. When expressed via Scer\GAL4^pnr-MD237 a dorsal band of pigment extends the length of the body from the occipital head region to the end of the abdomen but excluding the terminalia. In the thorax the piment extends from the midline to a longitudinal straight line defined by the dorsocentral bristles. When expressed via Scer\GAL4^ost-em462 pigment is expressed in a band adjacent to the pnr domain revealed by Scer\GAL4^pnr-MD237.

(Calleja et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^pnr-MD237, y^UAS.cCa has abnormal body color phenotype, enhanceable by e¹

(Wittkopp et al., 2002)

Suppressor of

Statement

Reference

y^UAS.cCa/Scer\GAL4^pnr-MD237 is a suppressor | partially of abnormal body color phenotype of Scer\GAL4^pnr-MD237, e^UAS.cWa

(Wittkopp et al., 2002)

Other

Statement

Reference

Scer\GAL4^pnr-MD237, t^UAS.cTa, y^UAS.cCa has abnormal body color phenotype

(Jeong et al., 2008)

Phenotype Manifest In

Other

Statement

Reference

Scer\GAL4^pnr-MD237, t^UAS.cTa, y^UAS.cCa has adult cuticle phenotype

(Jeong et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of both y^Scer\UAS.cCa and t^Scer\UAS.cTa under the control of Scer\GAL4^pnr-MD237 produces dramatic black pigmentation in the Scer\GAL4^pnr-MD237 expression domain of the adult abdomen in males.

(Jeong et al., 2008)

When y^Scer\UAS.cCa is driven by Scer\GAL4^pnr-MD237 in a e¹ background, a dramatic increase in black pigment in the thorax and in the abdomen. When y^Scer\UAS.cCa and e^Scer\UAS.cWa are driven together by Scer\GAL4^pnr-MD237 induces the formation of more black melanin than e^Scer\UAS.cWa alone, though the wild-type level of pigmentation is not restored.

(Wittkopp et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

y^UAS.cCa/Scer\GAL4^dsx-GAL4 rescues y¹

(Massey et al., 2019)

Scer\GAL4^dsx.KI/y^UAS.cCa rescues y¹

(Massey et al., 2019)

y^UAS.cCa/Scer\GAL4^c81 rescues y¹

(Drapeau et al., 2003)

y^UAS.cCa/Scer\GAL4^Act5C.PI rescues y¹

(Drapeau et al., 2003)

y^UAS.cCa/Scer\GAL4^hs.PB rescues y^unspecified

(Calleja et al., 1996)

Partially rescues

y^UAS.cCa, Scer\GAL80^nSyb.PS, Scer\GAL4^dsx-GAL4 partially rescues y¹

(Massey et al., 2019)

y^UAS.cCa/Scer\GAL4^y.PG partially rescues y¹

(Massey et al., 2019)

y^UAS.cCa/Scer\GAL4^pnr-MD237 partially rescues y^unspecified

(Jeong et al., 2008)

Fails to rescue

Scer\GAL4^fru.P1.D/y^UAS.cCa fails to rescue y¹

(Massey et al., 2019)

Comments

The expression of y^UAS.cCa under the control of Scer\GAL4^y.PG suppresses the yellow body color but not the male mating defects of y¹ mutants.

The expression of y^UAS.cCa under the control of Scer\GAL4^dsx-GAL4 alone or under the combined control of Scer\GAL4^dsx-GAL4 and Scer\GAL80^nSyb.PS fully and partially rescues, respectively, the male mating defects of y¹ mutants.

The expression of y^UAS.cCa under the control of Scer\GAL4^dsx.KI rescues both the body color and male mating defects of y¹ mutants.

(Massey et al., 2019)

Expression of y^Scer\UAS.cCa under the control of Scer\GAL4^pnr-MD237 in y^unspecified males rescues the y mutant phenotype only in the overlapping domain of Scer\GAL4^pnr-MD237 expression and the endogenous brown pigmentation.

(Jeong et al., 2008)

The reduced wing extension index (the percentage of each courtship ritual during which a male's wing is extended and vibrating) seen in y¹ males is rescued to roughly that of wild-type males by expression of y^Scer\UAS.cCa under the control of Scer\GAL4^Act5C.PI or Scer\GAL4^c81.

(Drapeau et al., 2003)

Images (0)

Mutant

Wild-type

Stocks (7)

Bloomington

3043

y¹ w¹¹¹⁸; P{UAS-y.C}MC1

36520

y¹ w^* P{GawB}fz3^MD810; P{UAS-y.C}MC1

3040

y¹ w¹¹¹⁸; P{GawB}tsh^md621/CyO; P{UAS-y.C}MC1/TM2