FB2024_03 , released June 25, 2024
Allele: Dmel\TieDN.UAS
Open Close
General Information
Symbol
Dmel\TieDN.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0280304
Feature type
allele
Associated gene
Dmel\Tie
Associated Insertion(s)
Carried in Construct
P{UAS-Tie.DN}
Key Links
Transgenic product class
dominant_negative_variant
(Wang et al., 2006)
inframe_deletion
(Wang et al., 2006)
Mutagen
Nature of the Allele
Transgenic product class
dominant_negative_variant
(Wang et al., 2006)
inframe_deletion
(Wang et al., 2006)
Mutagen
in vitro construct
(Wang et al., 2006)
Progenitor genotype
Carried in construct
P{UAS-Tie.DN}
Cytology
Description

UASt regulatory sequences drive expression of a dominant negative form of Tie which contains the signal sequence and extracellular and transmembrane domains, but lacks the intracellular kinase domain.

(Wang et al., 2006)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Tie
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of TieDN.Scer\UAS under the control of Scer\GAL4slbo.2.6 does not significantly alter the protrusion and retraction morphodynamics in border cell cluster migration.

      (Cai et al., 2014)

      Expression of TieDN.Scer\UAS under the control of Scer\GAL4c306 does not result in defects in border follicle cell migration.

      (Wang et al., 2006)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The addition of a dominant-negative TieDN.Scer\UAS to border cells expressing PvrDN.Scer\UAS and EgfrDN.Scer\UAS alters the protrusion and retraction morphodynamics of border cell cluster migration.

      (Cai et al., 2014)

      Co-expression of TieDN.Scer\UAS enhances the border cell migration defects caused by expression of PvrDN.Scer\UAS under the control of Scer\GAL4slbo.2.6, such that 37-58% of stage 10 egg chambers show border cell migration defects.

      Co-expression of PvrDN.Scer\UAS and EgfrDN.Scer\UAS under the control of Scer\GAL4c306 results in border cell migration defects. The severity of the migration defects is enhanced if the females also express TieDN.Scer\UAS.

      (Wang et al., 2006)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      TieDN.Scer\UAS
      TieDN.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (2)