FlyBase Allele Report: Scer\GAL4[path-c135]

ScerGAL4^path-c135 drives strong expression in carpet glial cells of the eye disc in third instar larvae; this expression is gradually diminished during pupal development.

(Yeh et al., 2018)

Drives expression in a number of tissues, including posterior follicle cells (stretch, polar, centripetal); the embryonic/larval proventriculus; embryonic/larval carpet glial cells; copper cells and Dh31 cell of the embryonic/larval midgut; and in several somatic tissues of the adult male reproductive system.

(FlyBase Curators, 2017)

Expressed in embryonic proventriculus, larval brain, eye disc, gut, fat body, adult ovarian squamous and posterior terminal follicle cells, male accessory glands, seminal vesicles, ejaculatory duct, cyst cells and spermatocytes.

(Kester and Nambu, 2011)

Expression in the midgut is found in a distinct group of Dh31-expressing enteroendocrine cells located at the junction region of the anterior midgut and the acid-secreting portion of the larval midgut. This junction region occurs at a conspicuous and characteristic U-shaped bend in the gut. These are referred to as the "midgut junction Dh31-expressing cells".

(Lajeunesse et al., 2010)

During oogenesis, ScerGAL4^c135 drives expression in the follicle cells at the posterior of the egg chamber at oogenesis stage 9-10, and in squamous nurse follicle cells and in centripetally migrating follicle cells at stage 10, and in posterior terminal cells. It does not drive expression in oocyte associated follicle cells. In the male reproductive tract, ScerGAL4^c135 drives expression in the male accessory gland secondary secretory cells, the seminal vesicle, the ejaculatory duct, cyst cells, and spermatocytes. In embryos, ScerGAL4^c135 drives expression in a ring-shaped pattern in the proventriculus. At larval stages, ScerGAL4^c135 drives expression in the brain, in glial cells in the eye disc, in the gut, and in the fat bodies.

(Hrdlicka et al., 2002)

Expression during embryogenesis has not yet been described. However, when used to drive expression of UAS constructs that are usually lethal when expressed in embryos, animals can be recovered. This suggests there is little or no embryonic expression.

(Perrimon, 1997.6.4)

Expression in the lymph gland is uniform.

(Harrison et al., 1995)

Marker for

antennal mechanosensory and motor center B1a projection neuron

(Lai et al., 2012)

Reflects expression of

path

(Kester and Nambu, 2011)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage, with Rho1^RNAi.UAS.cBa

(Ho et al., 2019)

abnormal neuroanatomy | third instar larval stage, with path^KK104735

(Ho et al., 2019)

abnormal neuroanatomy | third instar larval stage, with Rho1^RNAi.UAS.cBa

(Ho et al., 2019)

abnormal size | third instar larval stage, with upd3^UAS.cUa

(Ho et al., 2019)

lethal, with Pgant4^RNAi.UAS.WIZ

(Tran et al., 2012)

lethal, with Pgant5^GD903

(Tran et al., 2012)

lethal - all die before end of P-stage, with Ivir\M2^UAS.cAa

(Adamson et al., 2011)

male sterile, with Iswi^GD1467

(Doyen et al., 2015)

partially lethal - majority die, with Pgant9^RNAi.UAS.WIZ

(Tran et al., 2012)

some die during P-stage, with Ivir\M2^UAS.cAa

(Adamson et al., 2011)

Phenotype Manifest In

embryonic/larval carpet glial cell | third instar larval stage, with path^KK104735

(Ho et al., 2019)

embryonic/larval carpet glial cell | third instar larval stage, with Rho1^RNAi.UAS.cBa

(Ho et al., 2019)

embryonic/larval copper cell, with Pgant5^GD903

(Tran et al., 2012)

embryonic/larval gut, with Pgant5^GD903

(Tran et al., 2012)

embryonic/larval optic stalk surface glial cell | third instar larval stage, with Rho1^RNAi.UAS.cBa

(Ho et al., 2019)

eye disc | third instar larval stage, with path^KK104735

(Ho et al., 2019)

eye disc | third instar larval stage, with Rho1^RNAi.UAS.cBa

(Ho et al., 2019)

eye disc | third instar larval stage, with upd3^UAS.cUa

(Ho et al., 2019)

male germ cell nucleus, with mil^GD10622

(Doyen et al., 2015)

male germ cell nucleus, with Mst77F^GD10945

(Doyen et al., 2015)

perineurium | adult stage, with Rho1^RNAi.UAS.cBa

(Ho et al., 2019)

plasma membrane | third instar larval stage, with Rho1^RNAi.UAS.cBa

(Ho et al., 2019)

salivary gland | larval stage, with Pgant9^RNAi.UAS

(Ji et al., 2018)

secretory granule, with Pgant9^RNAi.UAS

(Ji et al., 2018)

spermatid, with Iswi^GD1467

(Doyen et al., 2015)

spermatid, with mil^GD10622

(Doyen et al., 2015)

spermatid, with Mst77F^GD10945

(Doyen et al., 2015)

spermatocyte | spermatogenesis, with aub^GD11831

(Bozzetti et al., 2015)

spermatocyte | spermatogenesis, with Fmr1^GD1288

(Bozzetti et al., 2015)

spermatozoon, with Iswi^GD1467

(Doyen et al., 2015)

spermatozoon, with mil^GD10622

(Doyen et al., 2015)

spermatozoon, with Mst77F^GD10945

(Doyen et al., 2015)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^path-c135, aub^GD11831 has spermatocyte | spermatogenesis phenotype, suppressible by Fmr1^UAS.cZa, Scer\GAL4^path-c135

(Bozzetti et al., 2015)

Pgant4^GD870, Scer\GAL4^path-c135 has secretory granule phenotype, suppressible by Tango1^UAS.cZa, Scer\GAL4^path-c135

(Zhang et al., 2014)

Pgant4^GD870, Scer\GAL4^path-c135 has embryonic/larval proventriculus outer layer phenotype, suppressible by Fur2^KK104000, Scer\GAL4^path-c135

(Zhang et al., 2014)

Pgant4^GD870, Scer\GAL4^path-c135 has secretory granule phenotype, suppressible by Fur2^KK104000, Scer\GAL4^path-c135

(Zhang et al., 2014)

Pgant4^RNAi.UAS.WIZ, Scer\GAL4^path-c135 has embryonic/larval proventriculus outer layer phenotype, suppressible by Tango1^UAS.cZa, Scer\GAL4^path-c135

(Zhang et al., 2014)

Pgant4^RNAi.UAS.WIZ, Scer\GAL4^path-c135 has secretory granule phenotype, suppressible by Tango1^UAS.cZa, Scer\GAL4^path-c135

(Zhang et al., 2014)

Pgant4^RNAi.UAS.WIZ, Scer\GAL4^path-c135 has embryonic/larval proventriculus outer layer phenotype, suppressible by Fur2^KK104000, Scer\GAL4^path-c135

(Zhang et al., 2014)

Pgant4^RNAi.UAS.WIZ, Scer\GAL4^path-c135 has secretory granule phenotype, suppressible by Fur2^KK104000, Scer\GAL4^path-c135

(Zhang et al., 2014)

Pgant4^GD870, Scer\GAL4^path-c135 has embryonic/larval proventriculus outer layer phenotype, suppressible by Tango1^UAS.cZa, Scer\GAL4^path-c135

(Zhang et al., 2014)

NOT suppressed by

Statement

Reference

Pgant4^GD870, Scer\GAL4^path-c135 has embryonic/larval proventriculus outer layer phenotype, non-suppressible by Fur1^GD12608, Scer\GAL4^path-c135

(Zhang et al., 2014)

Pgant4^GD870, Scer\GAL4^path-c135 has secretory granule phenotype, non-suppressible by Fur1^GD12608, Scer\GAL4^path-c135

(Zhang et al., 2014)

Pgant4^RNAi.UAS.WIZ, Scer\GAL4^path-c135 has embryonic/larval proventriculus outer layer phenotype, non-suppressible by Fur1^GD12608, Scer\GAL4^path-c135

(Zhang et al., 2014)

Pgant4^RNAi.UAS.WIZ, Scer\GAL4^path-c135 has secretory granule phenotype, non-suppressible by Fur1^GD12608, Scer\GAL4^path-c135

(Zhang et al., 2014)

Suppressor of

Statement

Reference

Fmr1^UAS.cZa, Scer\GAL4^path-c135 is a suppressor of spermatocyte | spermatogenesis phenotype of Scer\GAL4^path-c135, aub^GD11831

(Bozzetti et al., 2015)

aub^UAS.cBa/Scer\GAL4^path-c135 is a suppressor of spermatocyte | spermatogenesis phenotype of Fmr1^Δ113M

(Bozzetti et al., 2015)

Tango1^UAS.cZa, Scer\GAL4^path-c135 is a suppressor of secretory granule phenotype of Pgant4^GD870, Scer\GAL4^path-c135

(Zhang et al., 2014)

Fur2^KK104000, Scer\GAL4^path-c135 is a suppressor of embryonic/larval proventriculus outer layer phenotype of Pgant4^GD870, Scer\GAL4^path-c135

(Zhang et al., 2014)

Fur2^KK104000, Scer\GAL4^path-c135 is a suppressor of secretory granule phenotype of Pgant4^GD870, Scer\GAL4^path-c135

(Zhang et al., 2014)

Tango1^UAS.cZa, Scer\GAL4^path-c135 is a suppressor of embryonic/larval proventriculus outer layer phenotype of Pgant4^RNAi.UAS.WIZ, Scer\GAL4^path-c135

(Zhang et al., 2014)

Tango1^UAS.cZa, Scer\GAL4^path-c135 is a suppressor of secretory granule phenotype of Pgant4^RNAi.UAS.WIZ, Scer\GAL4^path-c135

(Zhang et al., 2014)

Fur2^KK104000, Scer\GAL4^path-c135 is a suppressor of embryonic/larval proventriculus outer layer phenotype of Pgant4^RNAi.UAS.WIZ, Scer\GAL4^path-c135

(Zhang et al., 2014)

Fur2^KK104000, Scer\GAL4^path-c135 is a suppressor of secretory granule phenotype of Pgant4^RNAi.UAS.WIZ, Scer\GAL4^path-c135

(Zhang et al., 2014)

Tango1^UAS.cZa, Scer\GAL4^path-c135 is a suppressor of embryonic/larval proventriculus outer layer phenotype of Pgant4^GD870, Scer\GAL4^path-c135

(Zhang et al., 2014)

NOT Suppressor of

Statement

Reference

Fur1^GD12608, Scer\GAL4^path-c135 is a non-suppressor of embryonic/larval proventriculus outer layer phenotype of Pgant4^GD870, Scer\GAL4^path-c135

(Zhang et al., 2014)

Fur1^GD12608, Scer\GAL4^path-c135 is a non-suppressor of secretory granule phenotype of Pgant4^GD870, Scer\GAL4^path-c135

(Zhang et al., 2014)

Fur1^GD12608, Scer\GAL4^path-c135 is a non-suppressor of embryonic/larval proventriculus outer layer phenotype of Pgant4^RNAi.UAS.WIZ, Scer\GAL4^path-c135

(Zhang et al., 2014)

Fur1^GD12608, Scer\GAL4^path-c135 is a non-suppressor of secretory granule phenotype of Pgant4^RNAi.UAS.WIZ, Scer\GAL4^path-c135

(Zhang et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Pgant9^B.UAS/Scer\GAL4^path-c135 rescues Pgant9^RNAi.UAS

(Ji et al., 2018)

Fmr1^UAS.cZa/Scer\GAL4^path-c135 rescues Fmr1^Δ113M/Fmr1[+]

(Bozzetti et al., 2015)

porin^UAS.cGa/Scer\GAL4^path-c135 rescues porin^Rev8

(Graham et al., 2010)

Fails to rescue

Pgant9^A.UAS/Scer\GAL4^path-c135 fails to rescue Pgant9^RNAi.UAS

(Ji et al., 2018)

Manf^UAS.cPa/Scer\GAL4^path-c135 fails to rescue Manf^Δ96

(Palgi et al., 2009)

tef^UAS.EGFP/Scer\GAL4^path-c135 fails to rescue tef^Z5864

(Arya et al., 2006)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

6978

w¹¹¹⁸; P{GawB}path^c135

Kyoto

108995

w¹¹¹⁸; P{GawB}path^c135

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

GAL4^c135

Scer\GAL4^c135

Scer\GAL4^path-c135

Name Synonyms

Secondary FlyBase IDs

References (25)

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