FlyBase Allele Report: Dmel\Pgant4[RNAi.UAS.WIZ]

General Information

Symbol

Dmel\Pgant4^RNAi.UAS.WIZ

Species

D. melanogaster

Name

FlyBase ID

FBal0277671

Feature type

allele

Associated gene

Dmel\Pgant4

Associated Insertion(s)

Carried in Construct

P{UAS-Pgant4.RNAi.WIZ}

Also Known As

UAS-pgant4IR

Key Links

Transgenic product class

RNAi_reagent

(Tran et al., 2012)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Tran et al., 2012)

Mutagen

in vitro construct

(Tran et al., 2012)

Progenitor genotype

Carried in construct

P{UAS-Pgant4.RNAi.WIZ}

Cytology

Description

UAS regulatory sequences drive expression of double-stranded RNA (dsRNA) as a snapback hairpin corresponding to an approximately 500 bp fragment of Pgant4 using a pWIZ vector.

(Tran et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Pgant4

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal, with Scer\GAL4^path-c135

(Tran et al., 2012)

lethal, with Scer\GAL4^αTub84B.PL

(Tran et al., 2012)

Phenotype Manifest In

embryonic/larval proventriculus outer layer, with Scer\GAL4^unspecified

(Zhang et al., 2014)

secretory granule, with Scer\GAL4^unspecified

(Zhang et al., 2014)

Detailed Description

Statement

Reference

The secretory cells (PR cells) of the proventriculus of larvae expressing pgant4^{dsRNA.Scer\UAS.WIZ} show disrupted morphology and secretion, are enlarged, and display loss of apical secretory components and secretory granules, compared to wild type.

(Zhang et al., 2014)

Expression of pgant4^{dsRNA.Scer\UAS.WIZ} under the control of Scer\GAL4^αTub84B.PL results in complete loss of viability.

Expression of pgant4^{dsRNA.Scer\UAS.WIZ} in the digestive system under the control of Scer\GAL4^c135 results in almost complete lethality.

Expression of pgant4^{dsRNA.Scer\UAS.WIZ} under the control of Scer\GAL4^Bx-MS1096 does not result in wing blistering.

(Tran et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

Pgant4^RNAi.UAS.WIZ, Scer\GAL4^path-c135 has embryonic/larval proventriculus outer layer phenotype, suppressible by Tango1^UAS.cZa, Scer\GAL4^path-c135

(Zhang et al., 2014)

Pgant4^RNAi.UAS.WIZ, Scer\GAL4^path-c135 has secretory granule phenotype, suppressible by Tango1^UAS.cZa, Scer\GAL4^path-c135

(Zhang et al., 2014)

Pgant4^RNAi.UAS.WIZ, Scer\GAL4^path-c135 has embryonic/larval proventriculus outer layer phenotype, suppressible by Fur2^KK104000, Scer\GAL4^path-c135

(Zhang et al., 2014)

Pgant4^RNAi.UAS.WIZ, Scer\GAL4^path-c135 has secretory granule phenotype, suppressible by Fur2^KK104000, Scer\GAL4^path-c135

(Zhang et al., 2014)

NOT suppressed by

Statement

Reference

Pgant4^RNAi.UAS.WIZ, Scer\GAL4^path-c135 has embryonic/larval proventriculus outer layer phenotype, non-suppressible by Fur1^GD12608, Scer\GAL4^path-c135

(Zhang et al., 2014)

Pgant4^RNAi.UAS.WIZ, Scer\GAL4^path-c135 has secretory granule phenotype, non-suppressible by Fur1^GD12608, Scer\GAL4^path-c135

(Zhang et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Tango1^Scer\UAS.cZa in PR cells via Scer\GAL4^c135 rescues the aberrant secretory apparatus structure and defective secretion seen in the PR cells of the proventriculus of pgant4^{dsRNA.Scer\UAS.WIZ}-expressing larvae.

Expression of Fur2^KK104000, but not Fur1^GD12608, in PR cells via Scer\GAL4^c135 rescues the aberrant secretory apparatus structure and defective secretion seen in the PR cells of the proventriculus of pgant4^{dsRNA.Scer\UAS.WIZ}-expressing larvae.

(Zhang et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Expression of pgant4^Scer\UAS.cZa, but not pgant4^m4.Scer\UAS, rescues the aberrant secretory apparatus structure and defective secretion seen in the PR cells of the proventriculus of pgant4 mutant larvae.

(Zhang et al., 2014)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Pgant4^RNAi.UAS.WIZ

Pgant4^{dsRNA.Scer\UAS.WIZ}

Pgant4^{dsRNA.UAS.WIZ}

pgant4^{dsRNA.Scer\UAS.WIZ}

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

Open Close