UASt regulatory sequences drive expression of an inverted repeat.
The secretory cells (PR cells) of the proventriculus of larvae expressing pgant4GD870 show disrupted morphology and secretion, are enlarged, and display loss of apical secretory components and secretory granules, compared to wild type.
Adults expressing pgant4GD870 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.
Expression under the control of Scer\GAL4Mef2.PR results in late pupal lethality.
Pgant4GD870, Scer\GAL4path-c135 has embryonic/larval proventriculus outer layer phenotype, suppressible by Tango1UAS.cZa, Scer\GAL4path-c135
Pgant4GD870, Scer\GAL4path-c135 has secretory granule phenotype, suppressible by Tango1UAS.cZa, Scer\GAL4path-c135
Pgant4GD870, Scer\GAL4path-c135 has embryonic/larval proventriculus outer layer phenotype, suppressible by Fur2KK104000, Scer\GAL4path-c135
Pgant4GD870, Scer\GAL4path-c135 has secretory granule phenotype, suppressible by Fur2KK104000, Scer\GAL4path-c135
Pgant4GD870, Scer\GAL4path-c135 has embryonic/larval proventriculus outer layer phenotype, non-suppressible by Fur1GD12608, Scer\GAL4path-c135
Pgant4GD870, Scer\GAL4path-c135 has secretory granule phenotype, non-suppressible by Fur1GD12608, Scer\GAL4path-c135
Expression of Tango1Scer\UAS.cZa in PR cells via Scer\GAL4c135 rescues the aberrant secretory apparatus structure and defective secretion seen in the PR cells of the proventriculus of pgant4GD870-expressing larvae.
Expression of Fur2KK104000, but not Fur1GD12608, in PR cells via Scer\GAL4c135 rescues the aberrant secretory apparatus structure and defective secretion seen in the PR cells of the proventriculus of pgant4GD870-expressing larvae.
Pgant4GD870 is rescued by Pgant4UAS.cZa/Scer\GAL4unspecified
Pgant4GD870 is not rescued by Scer\GAL4unspecified/Pgant4m2.UAS
Expression of pgant4Scer\UAS.cZa, but not pgant4m4.Scer\UAS, rescues the aberrant secretory apparatus structure and defective secretion seen in the PR cells of the proventriculus of pgant4 mutant larvae.