UASt regulatory sequences drive expression of an inverted repeat.
lethal, with Scer\GAL4c179
Expression of pgant5GD903 under the control of Scer\GAL4αTub84B.PL results in complete loss of viability.
Expression of pgant5GD903 in the digestive system under the control of Scer\GAL4c135 results in complete lethality.
Expression of pgant5GD903 in the embryonic mesoderm under the control of Scer\GAL4c179 results in complete lethality.
Expression of pgant5GD903 in the respiratory system under the control of Scer\GAL4btl.PS results in lethality.
The digestive tracts of larvae expressing pgant5GD903 under the control of Scer\GAL4c135 show an unusually basic pH compared with controls.
Wild-type copper cells display abundant O-glycoproteins throughout the apical and luminal region of copper cells. Larvae expressing pgant5c03193 under the control of Scer\GAL4c135 display a dramatic loss O-glycosylation normally found on copper cells and apical cytoskeletal abnormalities.
Expression of pgant5GD903 under the control of Scer\GAL4Bx-MS1096 does not result in wing blistering.
Adults expressing pgant5GD903 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.