FlyBase Insertion Report: Dmel\P{GawB}path[c135]

General Information

Symbol

Dmel\P{GawB}path^c135

Species

D. melanogaster

Name

FlyBase ID

FBti0002589

Feature type

transposable_element_insertion_site

Also Known As

C135-GAL4, c135, P{GawB}c135

Description

Inserted Element

P{GawB}

LINE ID

Affected gene(s)

path, Scer\GAL4

Viability / fertility

viable

Associated allele(s)

path^c135, Scer\GAL4^path-c135

Stock availability

2 publicly available

Genomic Location

Chromosomal Location

3L ( 67B10 )

Sequence Location

3L:9,504,820..9,504,820

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

Description

Detailed Mapping Data

Chromosome (arm)

Sequence Location

3L:9,504,820..9,504,820

(Ho et al., 2019)

Orientation

Cytological location (computed by FlyBase)

67B10 (inferred by FlyBase from sequence location)

Cytological location (reported)

Insertion into Natural transposon

Comments concerning location

The insertion was mapped to 5642bp upstream of the path translation start site.

(Ho et al., 2019)

Sequence Data

Flanking sequence

Inserted Element

Construct

P{GawB}

Size (Kb)

11.279

Component Allele(s)

Scer\GAL4^wB

w^+mW.hs

Molecular map

Affected Gene(s)

Insertion may affect gene

path

(Ho et al., 2019)

Scer\GAL4

(Lai et al., 2012, Tran et al., 2012, Adamson et al., 2011, Graham et al., 2010, Palgi et al., 2009, Arya et al., 2006, Hrdlicka et al., 2002, Harrison et al., 1995)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

path^c135

Phenotypic Class Phenotype Manifest in Detailed Description

Scer\GAL4^path-c135

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

embryonic/larval proventriculus

(Kester and Nambu, 2011)

embryonic/larval gut

(Kester and Nambu, 2011)

embryonic stage 16

embryonic proventriculus | restricted

(Hrdlicka et al., 2002)

third instar larval stage -- pupal stage P7

embryonic/larval carpet glial cell

(Yeh et al., 2018)

late third instar larval stage

embryonic/larval brain

(Hrdlicka et al., 2002)

embryonic/larval gut

(Hrdlicka et al., 2002)

glial cell of eye disc

(Hrdlicka et al., 2002)

embryonic/larval fat body

(Hrdlicka et al., 2002)

adult stage

neuron | subset of adult brain

(Lai et al., 2012)

antennal mechanosensory and motor center B1a projection neuron

(Lai et al., 2012)

adult stage | male

seminal vesicle

(Kester and Nambu, 2011, Hrdlicka et al., 2002)

male accessory gland secondary cell

(Hrdlicka et al., 2002)

ejaculatory duct

(Kester and Nambu, 2011, Hrdlicka et al., 2002)

cyst cell of testis

(Kester and Nambu, 2011, Hrdlicka et al., 2002)

spermatocyte

(Kester and Nambu, 2011, Hrdlicka et al., 2002)

male accessory gland

(Kester and Nambu, 2011)

adult stage | female

stretch follicle cell

(Kester and Nambu, 2011)

polar follicle cell

Comment: posterior

(Kester and Nambu, 2011)

adult stage | female & oogenesis

follicle cell

(Hartman et al., 2015)

oogenesis & adult stage | female

border follicle cell

(Hartman et al., 2015)

adult stage | female & oogenesis stage S10

stretch follicle cell

(Hrdlicka et al., 2002)

centripetal follicle cell

(Hrdlicka et al., 2002)

adult stage | female oogenesis stage S9 -- S10

follicle cell | posterior | subset

(Hrdlicka et al., 2002)

Additional Information

Statement

Reference

ScerGAL4^path-c135 drives strong expression in carpet glial cells of the eye disc in third instar larvae; this expression is gradually diminished during pupal development.

(Yeh et al., 2018)

Drives expression in a number of tissues, including posterior follicle cells (stretch, polar, centripetal); the embryonic/larval proventriculus; embryonic/larval carpet glial cells; copper cells and Dh31 cell of the embryonic/larval midgut; and in several somatic tissues of the adult male reproductive system.

(FlyBase Curators, 2017)

Expressed in embryonic proventriculus, larval brain, eye disc, gut, fat body, adult ovarian squamous and posterior terminal follicle cells, male accessory glands, seminal vesicles, ejaculatory duct, cyst cells and spermatocytes.

(Kester and Nambu, 2011)

Expression in the midgut is found in a distinct group of Dh31-expressing enteroendocrine cells located at the junction region of the anterior midgut and the acid-secreting portion of the larval midgut. This junction region occurs at a conspicuous and characteristic U-shaped bend in the gut. These are referred to as the "midgut junction Dh31-expressing cells".

(Lajeunesse et al., 2010)

During oogenesis, ScerGAL4^c135 drives expression in the follicle cells at the posterior of the egg chamber at oogenesis stage 9-10, and in squamous nurse follicle cells and in centripetally migrating follicle cells at stage 10, and in posterior terminal cells. It does not drive expression in oocyte associated follicle cells. In the male reproductive tract, ScerGAL4^c135 drives expression in the male accessory gland secondary secretory cells, the seminal vesicle, the ejaculatory duct, cyst cells, and spermatocytes. In embryos, ScerGAL4^c135 drives expression in a ring-shaped pattern in the proventriculus. At larval stages, ScerGAL4^c135 drives expression in the brain, in glial cells in the eye disc, in the gut, and in the fat bodies.

(Hrdlicka et al., 2002)

Expression during embryogenesis has not yet been described. However, when used to drive expression of UAS constructs that are usually lethal when expressed in embryos, animals can be recovered. This suggests there is little or no embryonic expression.

(Perrimon, 1997.6.4)

Expression in the lymph gland is uniform.

(Harrison et al., 1995)

Marker for

antennal mechanosensory and motor center B1a projection neuron

(Lai et al., 2012)

Reflects expression of

path

(Kester and Nambu, 2011)

Reporter construct used in assay

Mmus\Cd8a^UAS.cLa.EGFP

(Lai et al., 2012, Hrdlicka et al., 2002)

Data on Genetic Line

Line ID

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (2)

Bloomington

6978

w¹¹¹⁸; P{GawB}path^c135

Kyoto

108995

w¹¹¹⁸; P{GawB}path^c135

External Crossreferences and Linkouts ( 0 )

Comments

The orientation of the insertion was not reported.

(Ho et al., 2019)

Generated by K. Kaiser.

(Perrimon, 1997.6.4)

When directing expression of P{UAS-hop.Tum} or P{UAS-hop.H}, capable of producing melanotic tumors in larvae.

(Harrison et al., 1995)

Synonyms and Secondary IDs (12)

Reported As

Symbol Synonym

C135

(Lajeunesse et al., 2010, Arya et al., 2006)

C135-GAL4

(Chang et al., 2018, Yeh et al., 2018, Doyen et al., 2015, Palgi et al., 2009)

C135-Gal4

(Bestetti et al., 2021, Ho et al., 2019, Adamson et al., 2011, Lajeunesse et al., 2010)

C135c135-Gal4

(Hrdlicka et al., 2002)

E0583-Gal4

(Lai et al., 2012)

P{GawB}C135

(Perrimon, 1997.6.4)

P{GawB}c135

(Hartman et al., 2015, Kester and Nambu, 2011, FlyBase, 1992-)

P{GawB}path^c135

c135

(Zhang et al., 2017, Tran et al., 2012, Harrison et al., 1995)

c135-GAL4

(Biton et al., 2023, Graham et al., 2010)

c135-Gal4

(Reynolds et al., 2019, Ji et al., 2018, Bozzetti et al., 2015)

w¹¹¹⁸;P{w^+mW.hs=GawB}c135

(Lai et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (25)

Report Sections

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