FlyBase Allele Report: Dmel\Trl[R67]

General Information

Symbol

Dmel\Trl^R67

Species

D. melanogaster

Name

FlyBase ID

FBal0034116

Feature type

allele

Associated gene

Dmel\Trl

Associated Insertion(s)

Carried in Construct

Also Known As

Trl⁶⁷

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Farkas et al., 1994)

Progenitor genotype

Trl^13C

(Farkas et al., 1994)

Cytology

Description

Imprecise excision of the P-element and deletion of flanking DNA removing a section of the Trl transcription unit.

(Farkas et al., 1994)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

delins

3L:14,754,443..14,755,322 [-]

Comment:

Deletion resulting from imprecise P element excision. 17 base pairs remain from the 5' end of the transposon.

(Katokhin et al., 2001, Katokhin et al., 2001)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

homeotic (with Trl^109.2), with Trl^{Hsp83.GAGA-519}

(Greenberg and Schedl, 2001)

lethal (with Trl^13C)

(Greenberg and Schedl, 2001)

lethal (with Trl^109.2)

(Greenberg and Schedl, 2001)

lethal | recessive

(Granok et al., 2001, Katokhin et al., 2001)

lethal - all die before end of larval stage | recessive

(Farkas et al., 1994)

partially lethal - majority die | maternal effect (with Trl^109.2), with Trl^{Hsp83.GAGA-519}

(Greenberg and Schedl, 2001)

semi-fertile (with Trl^109.2), with Trl^{Hsp83.GAGA-519}

(Greenberg and Schedl, 2001)

some die during embryonic stage | maternal effect (with Trl^109.2), with Trl^{Hsp83.GAGA-519}

(Greenberg and Schedl, 2001)

some die during larval stage | recessive

(Farkas et al., 1994)

Phenotype Manifest In

abdominal segment 5 | ectopic (with Trl^109.2), with Trl^{Hsp83.GAGA-519}

(Greenberg and Schedl, 2001)

abdominal segment 6 (with Trl^109.2), with Trl^{Hsp83.GAGA-519}

(Greenberg and Schedl, 2001)

wing margin, with Trl^{Hsp83.GAGA.lacZ}

(Greenberg and Schedl, 2001)

Detailed Description

Statement

Reference

Homozygous Trl^13C mutant flies have reduced viability and this lethality is enhanced in Trl^13C/Trl^R67 heterozygotes, with only a few percent of the mutant flies surviving to the adult stage.

All surviving male flies expressing Trl^{Hsp83.GAGA-581} in a Trl^109.2/Trl^R67 mutant background show a transformation of the sixth abdominal segment towards the fifth.

Trl^109.2/Trl^R67 mutant females expressing four copies of Trl^{Hsp83.GAGA-581} exhibit egg laying defects, laying approximately 12 eggs per 24 hours, compared to around 25 in wild type.

Approximately 40% of embryos produced by Trl^109.2/Trl^R67 mutant females expressing four copies of Trl^{Hsp83.GAGA-581} are able to hatch. None of the embryos show any defects in nuclear division and the nuclear monolayer is intact.

Approximately 80% of Trl^13C/Trl^R67 mutant males show a transformation of the sixth abdominal segment towards the fifth.

Fewer than 20% of the embryos produced by homozygous Trl^13C/Trl^R67 mutant mothers survive to the larval stages, even when fertilised by wild type males.

While expression of a single copy of Trl^{Hsp83.GAGA.T:Ecol\lacZ} has little effect on viability, expression of two copies at high temperatures significantly reduced viability. The surviving flies exhibit phenotypes including loss of the wing margin. Adding one copy of Trl^R67 does not enhance the wing margin defects.

One copy of Trl^R67 does not enhance the wing margin defects seen when two copies of Trl^{Hsp83.GAGA.T:Ecol\lacZ} are expressed at high temperatures.

(Greenberg and Schedl, 2001)

Lethality occurs at metamorphosis.

(Katokhin et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Trl[+]/Trl^R67 is a suppressor of visible | recessive phenotype of sd^Fab-X

(Canudas et al., 2005)

Other

Statement

Reference

Df(3R)sbd45/+, Trl^R67 has visible | dominant phenotype

(Canudas et al., 2005)

Df(3R)sbd26/+, Trl^R67 has visible | dominant | homeotic phenotype

(Espinas et al., 2000)

Phenotype Manifest In

Suppressor of

Statement

Reference

Trl[+]/Trl^R67 is a suppressor of wing blade phenotype of sd^Fab-X

(Canudas et al., 2005)

Trl^R67 is a suppressor of eye phenotype of CycE^JP

(Brumby et al., 2002)

Other

Statement

Reference

Df(3R)sbd45/+, Trl^R67 has adult abdominal segment 6 phenotype

(Canudas et al., 2005)

Df(3R)sbd45/+, Trl^R67 has abdominal sternite bristle | ectopic phenotype

(Canudas et al., 2005)

Df(3R)sbd26/+, Trl^R67 has adult abdominal segment 6 phenotype

(Espinas et al., 2000)

Df(3R)sbd26/+, Trl^R67 has adult abdominal segment 5 | ectopic phenotype

(Espinas et al., 2000)

Df(3R)sbd26/+, Trl^R67 has abdominal sternite bristle | ectopic phenotype

(Espinas et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

56% of Trl^R67/Df(3R)sbd45 transheterozygous males show transformation of A6 to A5, as judged by the presence of at least one bristle on the sixth sternite.

Trl^R67/Bin1^R7-18 transheterozygous males do not show transformation of A6 to A5.

The wing blade destruction phenotype of homozygous sd^Fab-X females is significantly suppressed by Trl^R67/+ (32% show wing blade destruction in one wing, 51% show wing blade destruction in both wings).

(Canudas et al., 2005)

Mutant weakly suppresses the CycE^JP rough eye phenotype.

(Brumby et al., 2002)

Trl^R67/Df(3R)sbd26 double heterozygous males show a homeotic transformation of the sixth abdominal segment into the fifth, as shown by the presence of 6-8 bristles in the sixth sternite in most flies. This transformation is very infrequent in either single heterozygote, where no males are detected to have more than two bristles and many have no bristles at all on the sixth sternite.

(Espinas et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

Trl^R67/Trl^13C is partially rescued by Trl^hs.PG

(Granok et al., 2001)

Trl^R67/Trl^13C is partially rescued by Trl^{Hsp83.GAGA-581}

(Greenberg and Schedl, 2001)

Trl^R67/Trl^13C is partially rescued by Trl^{Hsp83.GAGA-519}

(Greenberg and Schedl, 2001)

Trl^R67/Trl^109.2 is partially rescued by Trl^{Hsp83.GAGA-581}

(Greenberg and Schedl, 2001)

Trl^R67/Trl^109.2 is partially rescued by Trl^{Hsp83.GAGA-519}/Trl^{Hsp83.GAGA-581}

(Greenberg and Schedl, 2001)

Not rescued by

Trl⁶²/Trl^R67 is not rescued by Trl^hs.PG

(Granok et al., 2001)

Trl^R67 is not rescued by Trl^hs.PG

(Granok et al., 2001)

Comments

Expression of Trl^{Hsp83.GAGA-519} partially rescues the lethality seen in Trl^13C/Trl^R67 mutants, with a survival rate of between 10 and 20% depending on the insertion line.

Expression of one or two copies of Trl^{Hsp83.GAGA-581} has no effect on the lethality associated with Trl^109.2/Trl^R67 mutants. However expression of four copies is able to partially rescue the phenotype, with 80% of flies surviving.

Expression of two copies of Trl^{Hsp83.GAGA-519} and two copies of Trl^{Hsp83.GAGA-581} partially rescues the lethality associated with Trl^109.2/Trl^R67 mutants, with approximately 70% of flies surviving.

Expression of Trl^{Hsp83.GAGA-581} partially rescues the lethality seen in Trl^13C/Trl^R67 mutants, with a survival rate of between 25 and 60% depending on the insertion line.

Expression of a single copy of Trl^{Hsp83.GAGA.T:Ecol\lacZ} rescues the zygotic lethality in Trl^13C/Trl^R67 mutants. However the A6 to A5 abdominal transformation or the maternal-effect lethality are rescued.

(Greenberg and Schedl, 2001)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

DfTrlR67

(Katokhin et al., 2001)

Trl⁶⁷

(Canudas et al., 2005, Espinas et al., 2000)

Trl^R67

(Liaw, 2022, Tang et al., 2022, Quijano et al., 2016, Greenberg and Schedl, 2001)

Name Synonyms

Secondary FlyBase IDs

References (11)

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