FB2024_03 , released June 25, 2024
Allele: Dmel\TrlHsp83.GAGA-581
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General Information
Symbol
Dmel\TrlHsp83.GAGA-581
Species
D. melanogaster
Name
FlyBase ID
FBal0280395
Feature type
allele
Associated gene
Dmel\Trl
Associated Insertion(s)
Carried in Construct
P{Hsp83-Trl.GAGA-581}
Also Known As
hsp83:GAGA-581
Key Links
Transgenic product class
wild_type
(Greenberg and Schedl, 2001)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Greenberg and Schedl, 2001)
Mutagen
in vitro construct
(Greenberg and Schedl, 2001)
Progenitor genotype
Carried in construct
P{Hsp83-Trl.GAGA-581}
Cytology
Description

A Hsp83 promoter drives expression of the GAGA-581 isoform of Trl.

(Greenberg and Schedl, 2001)
Allele components
Component
Use(s)
Regulatory region(s)
Hsp83
Encoded product / tool
Trl
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      All surviving male flies expressing TrlHsp83.GAGA-581 in a Trl109.2/TrlR67 mutant background show a transformation of the sixth abdominal segment towards the fifth.

      Trl109.2/TrlR67 mutant females expressing four copies of TrlHsp83.GAGA-581 exhibit egg laying defects, laying approximately 12 eggs per 24 hours, compared to around 25 in wild type.

      Approximately 40% of embryos produced by Trl109.2/TrlR67 mutant females expressing four copies of TrlHsp83.GAGA-581 are able to hatch. None of the embryos show any defects in nuclear division and the nuclear monolayer is intact.

      (Greenberg and Schedl, 2001)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Adding at least one copy of the TrlHsp83.GAGA-581 transgene allows the recovery of a much higher frequency of homozygous Trl13C escapers that are also heterozygous for lolalk02512 (up to approximately 80% when four copies of the transgene are added)

      (Faucheux et al., 2003)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      TrlHsp83.GAGA-581 rescues TrlR85/Trlen82

      (Ogienko et al., 2008)
      Partially rescues

      TrlHsp83.GAGA-581 partially rescues TrlR67/Trl13C

      (Greenberg and Schedl, 2001)

      TrlHsp83.GAGA-581 partially rescues TrlR67/Trl109.2

      (Greenberg and Schedl, 2001)

      TrlHsp83.GAGA-519/TrlHsp83.GAGA-581 partially rescues TrlR67/Trl109.2

      (Greenberg and Schedl, 2001)

      TrlHsp83.GAGA-519/TrlHsp83.GAGA-581 partially rescues Trl81.1/Trl109.2

      (Greenberg and Schedl, 2001)
      Fails to rescue

      TrlHsp83.GAGA-581 fails to rescue Trl13C

      (Greenberg and Schedl, 2001)
      Comments

      Expression of TrlHsp83.GAGA-581 rescues the egg chamber phenotypes seen in Trlen82/TrlR85 mutant females.

      (Ogienko et al., 2008)

      Expression of TrlHsp83.GAGA-581 partially rescues the lethality seen in Trl13C/TrlR67 mutants, with a survival rate of between 25 and 60% depending on the insertion line.

      Expression of one or two copies of TrlHsp83.GAGA-581 has no effect on the lethality associated with Trl109.2/TrlR67 mutants. However expression of four copies is able to partially rescue the phenotype, with 80% of flies surviving.

      Expression of two copies of TrlHsp83.GAGA-519 and two copies of TrlHsp83.GAGA-581 partially rescues the lethality associated with Trl109.2/Trl18.1 mutants, with approximately 40% of flies surviving.

      Expression of two copies of TrlHsp83.GAGA-519 and two copies of TrlHsp83.GAGA-581 partially rescues the lethality associated with Trl109.2/TrlR67 mutants, with approximately 70% of flies surviving.

      Expression of a single copy of TrlHsp83.GAGA-581 does not rescue the abdominal segment transformation seen in Trl13C mutant males.

      Expression of a single copy of TrlHsp83.GAGA-581 does not rescue the reduction in egg production seen in Trl13C mutant females.

      Expression of TrlHsp83.GAGA-581 does not rescue the lethality seen in embryos produced by homozygous Trl13C mutant mothers.

      (Greenberg and Schedl, 2001)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      TrlHsp83.GAGA-581
      Name Synonyms
      Secondary FlyBase IDs
        References (3)