FlyBase Allele Report: Dmel\Trl[Hsp83.GAGA-519]

General Information

Symbol

Dmel\Trl^{Hsp83.GAGA-519}

Species

D. melanogaster

Name

FlyBase ID

FBal0280396

Feature type

allele

Associated gene

Dmel\Trl

Associated Insertion(s)

Carried in Construct

P{Hsp83-Trl.GAGA-519}

Also Known As

hsp83:GAGA-519

Key Links

Transgenic product class

wild_type

(Greenberg and Schedl, 2001)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Greenberg and Schedl, 2001)

Mutagen

in vitro construct

(Greenberg and Schedl, 2001)

Progenitor genotype

Carried in construct

P{Hsp83-Trl.GAGA-519}

Cytology

Description

A Hsp83 promoter drives expression of the GAGA-519 isoform of Trl.

(Greenberg and Schedl, 2001)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp83

Encoded product / tool

Trl

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

abdominal segment 5 | ectopic, with Trl^81.1/Trl^109.2

(Greenberg and Schedl, 2001)

abdominal segment 5 | ectopic, with Trl^R67/Trl^109.2

(Greenberg and Schedl, 2001)

abdominal segment 6, with Trl^81.1/Trl^109.2

(Greenberg and Schedl, 2001)

abdominal segment 6, with Trl^R67/Trl^109.2

(Greenberg and Schedl, 2001)

Detailed Description

Statement

Reference

Approximately 20% of surviving male flies expressing Trl^{Hsp83.GAGA-519} in a Trl^109.2/Trl^18.1 mutant background show a transformation of the sixth abdominal segment towards the fifth.

Trl^109.2/Trl^18.1 mutant females expressing four copies of Trl^{Hsp83.GAGA-519} exhibit egg laying defects, laying approximately 12 eggs per 24 hours, compared to around 25 in wild type.

Approximately 70% of embryos produced by Trl^109.2/Trl^18.1 mutant females expressing four copies of Trl^{Hsp83.GAGA-519} are able to hatch.

(Greenberg and Schedl, 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Adding at least one copy of the Trl^{Hsp83.GAGA-519} transgene allows the recovery of a much higher frequency of homozygous Trl^13C escapers that are also heterozygous for lolal^k02512 (up to approximately 80% when four copies of the transgene are added).

(Faucheux et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Trl^{Hsp83.GAGA-519} rescues Trl^R85/Trl^ex15

(Dorogova et al., 2014)

Trl^{Hsp83.GAGA-519} rescues Trl³⁶²/Trl^R85

(Dorogova et al., 2014)

Trl^{Hsp83.GAGA-519} rescues Trl^R85/Trl^en82

(Ogienko et al., 2008)

Partially rescues

Trl^{Hsp83.GAGA-519} partially rescues Trl^R67/Trl^13C

(Greenberg and Schedl, 2001)

Trl^{Hsp83.GAGA-519}/Trl^{Hsp83.GAGA-581} partially rescues Trl^R67/Trl^109.2

(Greenberg and Schedl, 2001)

Trl^{Hsp83.GAGA-519} partially rescues Trl^81.1/Trl^109.2

(Greenberg and Schedl, 2001)

Trl^{Hsp83.GAGA-519}/Trl^{Hsp83.GAGA-581} partially rescues Trl^81.1/Trl^109.2

(Greenberg and Schedl, 2001)

Trl^{Hsp83.GAGA-519} partially rescues Trl^13C

(Greenberg and Schedl, 2001)

Comments

Expression of Trl^{Hsp83.GAGA-519} rescues the egg chamber phenotypes seen in Trl^en82/Trl^R85 mutant females.

(Ogienko et al., 2008)

Expression of Trl^{Hsp83.GAGA-519} partially rescues the lethality seen in Trl^13C/Trl^R67 mutants, with a survival rate of between 10 and 20% depending on the insertion line.

Expression of one or two copies of Trl^{Hsp83.GAGA-519} has no effect on the lethality associated with Trl^109.2/Trl^18.1 mutants. However expression of four copies is able to partially rescue the phenotype, with 10% of flies surviving.

Expression of two copies of Trl^{Hsp83.GAGA-519} and two copies of Trl^{Hsp83.GAGA-581} partially rescues the lethality associated with Trl^109.2/Trl^18.1 mutants, with approximately 40% of flies surviving.

Expression of two copies of Trl^{Hsp83.GAGA-519} and two copies of Trl^{Hsp83.GAGA-581} partially rescues the lethality associated with Trl^109.2/Trl^R67 mutants, with approximately 70% of flies surviving.

Expression of a single copy of Trl^{Hsp83.GAGA-519} partially rescues the abdominal segment transformation seen in Trl^13C mutant males.

Expression of a single copy of Trl^{Hsp83.GAGA-519} partially rescues the reduction in egg production seen in Trl^13C mutant females.

Expression of Trl^{Hsp83.GAGA-519} partially rescues the lethality seen in embryos produced by homozygous Trl^13C mutant mothers.

(Greenberg and Schedl, 2001)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Trl^{Hsp83.GAGA-519}

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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