Imprecise excision of the progenitor P{lacW}Trl362 insertion, resulting in a deletion (nucleotides deleted correspond to positions 1574-3075 of GenBank accession AJ225042). 15bp of the P-element 3' end remains.
TrlR85/Trlex15 males are unable to produce viable offspring. The testes are 2-4 times smaller than normal and in 90% of cases only early stage (2- and 4-cell) germline cysts are present. Most of the mutant males have empty seminal vesicles, but 5% have a single mature, motile sperm. Mitochondrial defects are seen starting from the spermatogonia stage, with the mitochondria being much larger than normal, having a pale matrix and lacking cristae in many areas. At later stages of spermatogenesis autophagosome formation and massive lysis of the cytoplasm is seen.
Defects in primordial germ cell (PGC) migration are seen in TrlR85/Trlex15 embryos. Some of the PGCs fail to migrate, and those that do are scattered throughout the embryo.
TrlR85/Trlex15 is rescued by TrlHsp83.GAGA-519
