FB2024_03 , released June 25, 2024
Allele: Dmel\TrlHsp83.GAGA.lacZ
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General Information
Symbol
Dmel\TrlHsp83.GAGA.lacZ
Species
D. melanogaster
Name
FlyBase ID
FBal0280397
Feature type
allele
Associated gene
Dmel\Trl
Associated Insertion(s)
Carried in Construct
P{Hsp83-Trl.GAGA.lacZ}
Key Links
Transgenic product class
inframe_deletion
(Greenberg and Schedl, 2001)
Mutagen
Nature of the Allele
Transgenic product class
inframe_deletion
(Greenberg and Schedl, 2001)
Mutagen
in vitro construct
(Greenberg and Schedl, 2001)
Progenitor genotype
Carried in construct
P{Hsp83-Trl.GAGA.lacZ}
Cytology
Description

The first 495 amino acids of the Trl GAGA-519 isoform are fused to lacZ. The resulting fusion protein retains only 13 of the 30 glutamine residues found in the Q domain.

(Greenberg and Schedl, 2001)
Allele components
Component
Use(s)
Regulatory region(s)
Hsp83
Encoded product / tool
Trl
Tagged with
lacZ
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      While expression of a single copy of TrlHsp83.GAGA.T:Ecol\lacZ has little effect on viability, expression of two copies at high temperatures significantly reduced viability. The surviving flies exhibit phenotypes including loss of the wing margin. Adding one copy of TrlR67 does not enhance the wing margin defects.

      One copy of TrlR67 does not enhance the wing margin defects seen when two copies of TrlHsp83.GAGA.T:Ecol\lacZ are expressed at high temperatures.

      (Greenberg and Schedl, 2001)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments

      Expression of a single copy of TrlHsp83.GAGA.T:Ecol\lacZ rescues the zygotic lethality in Trl13C/TrlR67 mutants. However the A6 to A5 abdominal transformation or the maternal-effect lethality are rescued.

      (Greenberg and Schedl, 2001)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      TrlHsp83.GAGA.T:Ecol\lacZ
      TrlHsp83.GAGA.lacZ
      Name Synonyms
      Secondary FlyBase IDs
        References (1)