FlyBase Allele Report: Dmel\Trl[hs.PG]

General Information

Symbol

Dmel\Trl^hs.PG

Species

D. melanogaster

Name

heat shock construct of Granok

FlyBase ID

FBal0130559

Feature type

allele

Associated gene

Dmel\Trl

Associated Insertion(s)

Carried in Construct

P{hs-Trl.G}

Key Links

Transgenic product class

cDNA

(Granok et al., 2001)

wild_type

(Granok et al., 2001)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Granok et al., 2001)

wild_type

(Granok et al., 2001)

Mutagen

in vitro construct

(Granok et al., 2001)

Progenitor genotype

Carried in construct

P{hs-Trl.G}

Cytology

Description

An Hsp70 promoter drives expression of a cDNA encoding the 519 isoform of Trl.

(Granok et al., 2001)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

Encoded product / tool

Trl

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

non-suppressor of variegation, with In(1)w^m4

(Granok et al., 2001)

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

Trl^hs.PG partially rescues Trl^R67/Trl^13C

(Granok et al., 2001)

Trl^hs.PG partially rescues Trl^13C

(Granok et al., 2001)

Fails to rescue

Trl^hs.PG fails to rescue Trl⁶²/Trl^13C

(Granok et al., 2001)

Trl^hs.PG fails to rescue Trl⁶²/Trl^R67

(Granok et al., 2001)

Trl^hs.PG fails to rescue Trl^R85/Trl⁶²

(Granok et al., 2001)

Trl^hs.PG fails to rescue Trl⁶²

(Granok et al., 2001)

Trl^hs.PG fails to rescue Trl^R67

(Granok et al., 2001)

Trl^hs.PG fails to rescue Trl^R85

(Granok et al., 2001)

Comments

Expression of Trl^hs.PG using heat shock at 37^oC fails to rescue the lethality of Trl⁶², Trl^R85 or Trl^R67 homozygotes or of Trl⁶²/Trl^R85, Trl⁶²/Trl^R67 or Trl⁶²/Trl^13C flies. Expression of Trl^hs.PG using heat shock at 37^oC slightly rescues the lethality of Trl^13C homozygotes; 0.41% homozygotes out of the expected 33% of total progeny are recovered. Expression of Trl^hs.PG using heat shock at 37^oC slightly rescues the lethality of Trl^13C/Trl^R67 flies, approximately 5% of the expected progeny are produced. Trl^13C/Trl^13C and Trl^13C/Trl^R67 rescued adults can survive at room temperature for no longer than 3 days and are sterile. Expression of Trl^hs.PG using heat shock at 37^oC slightly shifts the lethal phase of Trl⁶²/Trl⁶² homozygotes; the percentage of homozygous pupae is increased to 12% with heat shock treatment compared to 7% without heat shock.

(Granok et al., 2001)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Trl^hs.PG

Name Synonyms

heat shock construct of Granok

(Granok et al., 2001)

Secondary FlyBase IDs

References (1)

Report Sections

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