FlyBase Allele Report: Dmel\Cdk1[E1-24]

When cdc2^B47/cdc2^E1-24 animals are raiseds at a semi-restrictive temperature (26^oC), flies develop until the end of pupariation, but fail to hatch. These animals exhibit a severe loss of bristles. In addition, at 26h APF about 50% of sensory organs are composed of only two cells, lacking shaft and socket cells.

(Fichelson and Gho, 2004)

The average size of adult wing cells in homozygous clones which reach differentiation (after passing different times at the restrictive temperature) is twice (102.4%) that of control cells. Wing sectors carrying a mutant clone show an average reduction in area of 10.6% compared to controls. The average reduction in cell number for a sector carrying a mutant clone is 39.6% compared to controls. In addition to the reduction in area of the sector containing a mutant clone (autonomous effect), there is generally a reduction in the area of non-mutant sectors of the same wing (non-autonomous effect), the average reduction being 5.1% compared to controls. In 9/72 cases studied, the non-mutant territories show a slight increase in size, particularly sector C of the wing when the clone is in the P compartment. Homozygous clones in the notum fail to differentiate into chaetae, but they can differentiate to produce several trichomes per cell.

(Resino and Garcia-Bellido, 2004)

Embryos derived from homozygous females and raised at the restrictive temperature of 29^oC have severe cell cycle defects.

(Tadros et al., 2003)

cdc2^B47/cdc2^E1-24 larvae at the restrictive temperature show mitotic defects resulting in a reduction in the size of the imaginal discs and a reduction in the cell density in the optic lobe. The nuclei of the abdominal histoblasts are enlarged, and the DNA content is increased to about 16C.

(Hayashi and Yamaguchi, 1999)

cdc2^B47/cdc2^E1-24 females cease to lay eggs when shifted to the restrictive temperature of 29^oC. Dividing cells in the ovary disappear over a period of 5 days after shifting to 29^oC, while nurse cell nuclei persist and increase in size.

(Reed and Orr-Weaver, 1997)

Mitotic figures are not present in the wings of homozygous pupae 16 hours after being shifted to the restrictive temperature of 30^oC. Wings of homozygous pupae shifted to the restrictive temperature at the beginning of pupariation are of normal size 30 hours after being shifted to the restrictive temperature, and they are composed of cells which are larger than those of wild-type pupal wings raised under the same conditions. The pattern of DNA replication is normal in the pupal wings of homozygotes shifted to the restrictive temperature at pupariation. Homozygous clones in the wing induced during the second larval instar stage and shifted to the restrictive temperature at pupariation consist of cells with larger nuclei than the surrounding wild-type cells. The homozygous cdc2^E1-24 cells appear to contribute normally to the adult wing, although they sometimes produce large, clustered trichomes. cdc2^E1-24 flies raised at the restrictive temperature from pupariation onwards and expressing cdc2^Scer\UAS.cWa under the control of Scer\GAL4^en-e16E do not eclose, but the pattern of vein and intervein regions is normal 30 hours after shifting to the restrictive temperature. The anterior compartment of the wing disc is normal in size, but is composed of fewer and larger cells compared to the posterior compartment. cdc2^E1-24 larvae in which cdc2^Scer\UAS.cWa is expressed under the control of Scer\GAL4^en-e16E shortly after the moult to third instar and then the larvae are raised at the restrictive temperature of 30^oC have wing discs with fewer but very much larger cells in the anterior compartment compared to the posterior compartment.

(Weigmann et al., 1997)

The cdc2^B47/cdc2^E1-24 combination is temperature sensitive. cdc2^B47/cdc2^E1-24 third instar mutants the size of the central nervous system and imaginal discs is severly reduced. Many of the mutants cells in the CNS, salivary gland and imaginal discs are larger and more intensely DAPI-staining than wild type. Cell division is reduced but BrdU incorporation occurs in the salivary gland imaginal ring and CNS during the third instar. Abdominal histoblasts in cdc2^B47/cdc2^E1-24 larvae accumulate BrdU without increasing cell number, suggesting they are in endocycle.

(Hayashi, 1996)

The cdc2^E1-24/cdc2^216P combination shows temperature sensitivity: at 29^oC flies fail to eclose, while at 25^oC viable adults with abdominal defects eclose. Mutations at Cdc37 dominantly enhance this abdominal phenotype, causing profound derangement of abdominal structures, missing bristles, and fused segments. Mutations at Cdc37 also reduced the temperature at which the inviability of cdc2^E1-24/cdc2^216P takes effect.

(Cutforth and Rubin, 1994)

At the restrictive temperature they die after pupariation at the larval-pupal interface. Larvae take 1-2 days longer than wild type to reach the wandering stage. Imaginal discs are missing or rudimentary and larval brains are reduced in size. Endoreplication and polytenization of larval cells is normal, as are salivary gland size and intensity of DNA staining of their polytene nuclei. This ts allele was used to demonstrate that the maternal contribution of cdc2 is required for embryonic syncytial nuclear divisions: persistent cyclin A expression in PNS is observed.

(Stern et al., 1993)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Cdk1^E1-24 is an enhancer of visible phenotype of Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH

(Gupta et al., 2008)

Cdk1^E1-24 is an enhancer of abnormal size phenotype of Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH

(Gupta et al., 2008)

Suppressor of

Statement

Reference

cdc2[+]/Cdk1^E1-24 is a suppressor of increased cell death phenotype of shtd¹

(Tanaka-Matakatsu et al., 2007)

Phenotype Manifest In

Suppressed by

Statement

Reference

Cdk1^E1-24/Cdk1^B47 has histoblast & nucleus phenotype, suppressible by Xlae\cdc2^UAS.cHa/Scer\GAL4^P127

(Hayashi and Yamaguchi, 1999)

Cdk1^E1-24/Cdk1^B47 has histoblast & nucleus phenotype, suppressible by Scer\GAL4^P127/CycA^UAS.cHa/Xlae\cdc2^K33R.UAS

(Hayashi and Yamaguchi, 1999)

Cdk1^E1-24/Cdk1^B47 has histoblast & nucleus phenotype, suppressible by Scer\GAL4^P127/CycA^UAS.cHa

(Hayashi and Yamaguchi, 1999)

Cdk1^E1-24/Cdk1^B47 has imaginal disc phenotype, suppressible by Xlae\cdc2^UAS.cHa/Scer\GAL4^P127

(Hayashi and Yamaguchi, 1999)

Cdk1^E1-24/Cdk1^B47 has embryonic/larval optic lobe phenotype, suppressible by Xlae\cdc2^UAS.cHa/Scer\GAL4^P127

(Hayashi and Yamaguchi, 1999)

NOT suppressed by

Statement

Reference

Cdk1^E1-24/Cdk1^B47 has imaginal disc phenotype, non-suppressible by Scer\GAL4^P127/Xlae\cdc2^K33R.UAS

(Hayashi and Yamaguchi, 1999)

Cdk1^E1-24/Cdk1^B47 has embryonic/larval optic lobe phenotype, non-suppressible by Scer\GAL4^P127/Xlae\cdc2^K33R.UAS

(Hayashi and Yamaguchi, 1999)

Cdk1^E1-24/Cdk1^B47 has histoblast & nucleus phenotype, non-suppressible by Scer\GAL4^P127/Xlae\cdc2^K33R.UAS

(Hayashi and Yamaguchi, 1999)

Cdk1^E1-24/Cdk1^B47 has imaginal disc phenotype, non-suppressible by Scer\GAL4^P127/CycA^UAS.cHa/Xlae\cdc2^K33R.UAS

(Hayashi and Yamaguchi, 1999)

Enhancer of

Statement

Reference

Cdk1^E1-24 is an enhancer of eye phenotype of Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH

(Gupta et al., 2008)

Suppressor of

Statement

Reference

cdc2[+]/Cdk1^E1-24 is a suppressor of eye phenotype of shtd¹

(Tanaka-Matakatsu et al., 2007)

cdc2[+]/Cdk1^E1-24 is a suppressor of ommatidium phenotype of shtd¹

(Tanaka-Matakatsu et al., 2007)

NOT Suppressor of

Statement

Reference

cdc2[+]/Cdk1^E1-24 is a non-suppressor of phenotype of CycB^+t10

(Ji et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

The defects in the abdominal histoblasts are partially rescued by CycA^Scer\UAS.cHa expressed under the control of Scer\GAL4^P127; the increase in DNA content is limited to 8C.

(Hayashi and Yamaguchi, 1999)

The disc overproliferation phenotype of wts^e26-1 homozygotes is suppressed by two copies of cdc2^E1-24 at room temperature.

(Tao et al., 1999)

Mutations at esg have no further effect on the cdc2^B47/cdc2^E1-24 mutant phenotypes.

(Hayashi, 1996)

Xenogenetic Interactions

Statement

Reference

cdc2^E1-24 enhances the eye phenotypes seen when Mmus\Shisa5^Scer\UAS.cGa is expressed under the control of Scer\GAL4^dpp.PH.

(Gupta et al., 2008)

The defects of cdc2^B47/cdc2^E1-24 larvae are rescued by Xlae\cdc2^Scer\UAS.cHa expressed under the control of Scer\GAL4^P127, but not by Xlae\cdc2^{K33R.Scer\UAS} expressed under the control of Scer\GAL4^P127. Coexpression of CycA^Scer\UAS.cHa and Xlae\cdc2^{K33R.Scer\UAS} under the control of Scer\GAL4^P127 completely rescues the abdominal histoblast defects seen in cdc2^B47/cdc2^E1-24 larvae at the restrictive temperature. The imaginal disc defects seen in these larvae are not rescued.

(Hayashi and Yamaguchi, 1999)

Complementation and Rescue Data

Rescued by

Cdk1^E1-24/Cdk1^B47 is rescued by Cdk1^{WT.UASp.Venus}/Scer\GAL4^Tub.PU

(Ayeni et al., 2014)

Cdk1^E1-24/Cdk1^B47 is rescued by Cdk1^{T14A.UASp.Venus}/Scer\GAL4^Tub.PU

(Ayeni et al., 2014)

Not rescued by

Cdk1^E1-24/Cdk1^B47 is not rescued by Scer\GAL4^Tub.PU/Cdk1^{Y15F.UASp.Venus}

(Ayeni et al., 2014)

Cdk1^E1-24/Cdk1^B47 is not rescued by Scer\GAL4^Tub.PU/Cdk1^{T14A.Y15F.UASp.Venus}

(Ayeni et al., 2014)

Comments

Scer\GAL4^tub.PU-mediated expression of cdc2^{WT.Scer\UAS.P\T.T:Avic\GFP-YFP.Venus} rescues the pupal lethality of cdc2^B47/cdc2^E1-24 animals grown at the restrictive temperature of 25[o]C.

Scer\GAL4^tub.PU-mediated expression of cdc2^{T14A.Scer\UAS.P\T.T:Avic\GFP-YFP.Venus} rescues the pupal lethality of cdc2^B47/cdc2^E1-24 animals grown at the restrictive temperature of 25[o]C.

Scer\GAL4^tub.PU-mediated expression of cdc2^{Y15F.Scer\UAS.P\T.T:Avic\GFP-YFP.Venus} does not rescue the pupal lethality of cdc2^B47/cdc2^E1-24 animals grown at the restrictive temperature of 25[o]C.

Scer\GAL4^tub.PU-mediated expression of cdc2^{T14A.Y15F.Scer\UAS.P\T.T:Avic\GFP-YFP.Venus} does not rescue the pupal lethality of cdc2^B47/cdc2^E1-24 animals grown at the restrictive temperature of 25[o]C.

(Ayeni et al., 2014)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

6641

Cdk1^E1-24 b¹ pr¹ cn¹/CyO, P{ftz-lacB}E3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Crossreferences

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

S66805

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

Cdc2^E1-24

(Das et al., 2016, Weigmann et al., 1997)

Cdk1^E1-24

(Swan et al., 2005)

Dmcdc2^E1-24

(Nakajima et al., 2011, Resino and Garcia-Bellido, 2004, Reed and Orr-Weaver, 1997, Cutforth and Rubin, 1994)

cdc2^E1-24

(Gupta et al., 2008, Von Stetina et al., 2008, Tanaka-Matakatsu et al., 2007)

cdc2^E1-E24

(Ayeni et al., 2014, Fichelson and Gho, 2004)

cdc2^ts

(Ayeni et al., 2014)

cdk1^E1-24

(Tadros et al., 2003, Ji et al., 2002)

Name Synonyms

Secondary FlyBase IDs

References (19)

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