FB2024_03 , released June 25, 2024
Allele: Dmel\shtd1
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General Information
Symbol
Dmel\shtd1
Species
D. melanogaster
Name
shattered1
FlyBase ID
FBal0140936
Feature type
allele
Associated gene
Dmel\shtd
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Tanaka-Matakatsu et al., 2007)
Progenitor genotype
Cytology
Description

Amino acid replacement: E1184K.

(Tanaka-Matakatsu et al., 2007)

Single amino acid substitution.

(Tanaka-Matakatsu and Thomas, 2003)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
X:15,458,551..15,458,551 [-]
Nucleotide change:

G15458551A

Amino acid change:

E1184K | shtd-PA

Reported amino acid change:

E1184K

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Tanaka-Matakatsu et al., 2007)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      shtd1 mutant flies exhibit small eyes with highly disorganised ommatidia.

      shtd1 mutants fail to undergo G1 cell cycle arrest in the morphogenetic furrow and exhibit additional cell divisions of the differentiating photoreceptor cells. In adiition the shtd1 mutant is also defective in progression through mitosis and shows significantly increased apoptosis.

      shtd1 mutant eye discs show disruptions in both the numbers of neuronal cells in the developing clusters and in spacing of the clusters. Two R8-positive nuclei are seen in each photoreceptor cell cluster in shtd1 mutants, instead of a single nucleus as in wild-type. More posteriorly, after the morphogenetic furrow, wild-type eye discs contain single R8 cells, while shtd1 mutant discs contain R8 doublets.

      Multiple types of photoreceptor cells undergo additional rounds of cell division in shtd1 mutants. Despite the observed additional mitosis, a decreased number of R3 and R4 photoreceptor cells are observed.

      Cells enter S phase precociously in the G1 arrest domain in shtd1 eye discs.

      There is a dramatic increase in apoptosis in the posterior of developing shtd1 eye discs.

      An average of approximately 59 mitotic cells are seen in and behind the morphogenetic furrow in shtd1 mutant eye discs as compared to approximately 17 in wild-type. The number of mitotic cells anterior to the morphogenetic furrow is also slightly increased in shtd1 mutants as compared to that in wild-type (18 versus 10, respectively) while no difference is observed in the number of S phase cells in the mutant as compared to wild-type anterior to the morphogenetic furrow.

      (Tanaka-Matakatsu et al., 2007)

      Mutants have a rough eye phenotype and a severe photoreceptor differentiation defect. Mutants show M phase arrest and premature S phase entry phenotypes.

      (Tanaka-Matakatsu and Thomas, 2003)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Statement
      Reference

      shtd1 has increased cell death phenotype, enhanceable by CycH[+]/CycHEP3658

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has increased cell death phenotype, enhanceable by Diap14

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has increased cell death phenotype, enhanceable by Diap15

      (Tanaka-Matakatsu et al., 2007)
      Suppressed by
      Statement
      Reference

      shtd1 has increased cell death phenotype, suppressible by stgAR2/stg[+]

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has increased cell death phenotype, suppressible by stgRXT13/stg[+]

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has increased cell death phenotype, suppressible by stg4/stg[+]

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has increased cell death phenotype, suppressible by stg2/stg[+]

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has increased cell death phenotype, suppressible by CycA[+]/CycA5

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has increased cell death phenotype, suppressible by CycAC8LR1/CycA[+]

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has increased cell death phenotype, suppressible by CycA[+]/CycA03946

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has increased cell death phenotype, suppressible by cdc2[+]/Cdk1B47

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has increased cell death phenotype, suppressible by cdc2[+]/Cdk1E10

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has increased cell death phenotype, suppressible by cdc2[+]/Cdk1E1-24

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has increased cell death phenotype, suppressible by cdc2[+]/Cdk1E51Q

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has increased cell death phenotype, suppressible by mr[+]/mr1

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has increased cell death phenotype, suppressible by mr[+]/mr2

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has increased cell death phenotype, suppressible by lmg[+]/lmgA03424

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has increased cell death phenotype, suppressible by ida[+]/idaB4

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has increased cell death phenotype, suppressible by ida[+]/idaD14

      (Tanaka-Matakatsu et al., 2007)
      NOT suppressed by
      Statement
      Reference

      shtd1 has increased cell death phenotype, non-suppressible by CycA[+]/CycAhari

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has increased cell death phenotype, non-suppressible by CycA3/CycA[+]

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has increased cell death phenotype, non-suppressible by CycB3L6540/CycB3[+]

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has increased cell death phenotype, non-suppressible by CycEAR95/CycE[+]

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has increased cell death phenotype, non-suppressible by CycE[+]/CycE05206

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has increased cell death phenotype, non-suppressible by CycE[+]/CycEJP

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has increased cell death phenotype, non-suppressible by Cdk4k06503/Cdk4[+]

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has increased cell death phenotype, non-suppressible by Cdk4[+]/Cdk405428

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has increased cell death phenotype, non-suppressible by Cdk4[+]/Cdk4s4639

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has increased cell death phenotype, non-suppressible by Cdc27[+]/Cdc27L7123

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has increased cell death phenotype, non-suppressible by fzy[+]/fzy1

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has increased cell death phenotype, non-suppressible by fzy[+]/fzy4

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has increased cell death phenotype, non-suppressible by fzy[+]/fzy5

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has increased cell death phenotype, non-suppressible by fzy[+]/fzy6

      (Tanaka-Matakatsu et al., 2007)
      Phenotype Manifest In
      Enhanced by
      Statement
      Reference

      shtd1 has eye phenotype, enhanceable by CycH[+]/CycHEP3658

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has ommatidium phenotype, enhanceable by CycH[+]/CycHEP3658

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has eye phenotype, enhanceable by Diap14

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has ommatidium phenotype, enhanceable by Diap14

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has eye phenotype, enhanceable by Diap15

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has ommatidium phenotype, enhanceable by Diap15

      (Tanaka-Matakatsu et al., 2007)
      Suppressed by
      Statement
      Reference

      shtd1 has eye phenotype, suppressible by stgAR2/stg[+]

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has eye phenotype, suppressible by stgRXT13/stg[+]

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has eye phenotype, suppressible by stg4/stg[+]

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has eye phenotype, suppressible by stg2/stg[+]

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has ommatidium phenotype, suppressible by stgAR2/stg[+]

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has ommatidium phenotype, suppressible by stgRXT13/stg[+]

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has ommatidium phenotype, suppressible by stg4/stg[+]

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has ommatidium phenotype, suppressible by stg2/stg[+]

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has eye phenotype, suppressible by CycA[+]/CycA5

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has eye phenotype, suppressible by CycAC8LR1/CycA[+]

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has eye phenotype, suppressible by CycA[+]/CycA03946

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has ommatidium phenotype, suppressible by CycA[+]/CycA5

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has ommatidium phenotype, suppressible by CycAC8LR1/CycA[+]

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has ommatidium phenotype, suppressible by CycA[+]/CycA03946

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has eye phenotype, suppressible by cdc2[+]/Cdk1B47

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has ommatidium phenotype, suppressible by cdc2[+]/Cdk1B47

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has eye phenotype, suppressible by cdc2[+]/Cdk1E10

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has ommatidium phenotype, suppressible by cdc2[+]/Cdk1E10

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has eye phenotype, suppressible by cdc2[+]/Cdk1E1-24

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has ommatidium phenotype, suppressible by cdc2[+]/Cdk1E1-24

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has eye phenotype, suppressible by cdc2[+]/Cdk1E51Q

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has ommatidium phenotype, suppressible by cdc2[+]/Cdk1E51Q

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has eye phenotype, suppressible by mr[+]/mr1

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has ommatidium phenotype, suppressible by mr[+]/mr2

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has eye phenotype, suppressible by lmg[+]/lmgA03424

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has ommatidium phenotype, suppressible by lmg[+]/lmgA03424

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has eye phenotype, suppressible by ida[+]/idaB4

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has ommatidium phenotype, suppressible by ida[+]/idaB4

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has eye phenotype, suppressible by ida[+]/idaD14

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has ommatidium phenotype, suppressible by ida[+]/idaD14

      (Tanaka-Matakatsu et al., 2007)
      NOT suppressed by
      Statement
      Reference

      shtd1 has eye phenotype, non-suppressible by CycA[+]/CycAhari

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has eye phenotype, non-suppressible by CycA3/CycA[+]

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has eye phenotype, non-suppressible by CycE[+]/CycEJP

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has ommatidium phenotype, non-suppressible by CycE[+]/CycEJP

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has eye phenotype, non-suppressible by Cdk4k06503/Cdk4[+]

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has ommatidium phenotype, non-suppressible by Cdk4k06503/Cdk4[+]

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has eye phenotype, non-suppressible by Cdk4[+]/Cdk405428

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has ommatidium phenotype, non-suppressible by Cdk4[+]/Cdk405428

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has eye phenotype, non-suppressible by Cdk4[+]/Cdk4s4639

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has ommatidium phenotype, non-suppressible by Cdk4[+]/Cdk4s4639

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has eye phenotype, non-suppressible by Cdc27[+]/Cdc27L7123

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has ommatidium phenotype, non-suppressible by Cdc27[+]/Cdc27L7123

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has ommatidium phenotype, non-suppressible by CycA[+]/CycAhari

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has eye phenotype, non-suppressible by fzy[+]/fzy1

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has eye phenotype, non-suppressible by fzy[+]/fzy4

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has eye phenotype, non-suppressible by fzy[+]/fzy5

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has eye phenotype, non-suppressible by fzy[+]/fzy6

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has ommatidium phenotype, non-suppressible by fzy[+]/fzy1

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has ommatidium phenotype, non-suppressible by fzy[+]/fzy4

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has ommatidium phenotype, non-suppressible by fzy[+]/fzy5

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has ommatidium phenotype, non-suppressible by fzy[+]/fzy6

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has ommatidium phenotype, non-suppressible by CycA3/CycA[+]

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has eye phenotype, non-suppressible by CycB3L6540/CycB3[+]

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has ommatidium phenotype, non-suppressible by CycB3L6540/CycB3[+]

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has eye phenotype, non-suppressible by CycEAR95/CycE[+]

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has ommatidium phenotype, non-suppressible by CycEAR95/CycE[+]

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has eye phenotype, non-suppressible by CycE[+]/CycE05206

      (Tanaka-Matakatsu et al., 2007)

      shtd1 has ommatidium phenotype, non-suppressible by CycE[+]/CycE05206

      (Tanaka-Matakatsu et al., 2007)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      rux3 shtd1 double mutant eye discs exhibit more severe defects of aberrant mitotic figures and abnormal ommatidial spacing that that seen in either rux3 or shtd1 alone.

      (Tanaka-Matakatsu et al., 2007)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      shtd1
      (Tanaka-Matakatsu and Thomas, 2003)
      shtd1
      (Weber and Mlodzik, 2017, Popodi et al., 2010-, Tanaka-Matakatsu et al., 2007)
      Name Synonyms
      shattered1
      (Tanaka-Matakatsu et al., 2007)
      Secondary FlyBase IDs
        References (4)