FlyBase Allele Report: Dmel\stg[2]

General Information

Symbol

Dmel\stg²

Species

D. melanogaster

Name

FlyBase ID

FBal0247234

Feature type

allele

Associated gene

Dmel\stg

Associated Insertion(s)

Carried in Construct

Also Known As

stg^7B, stg^7B69, stg⁷

Key Links

Allele class

loss of function allele

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Reuter et al., 1993)

loss of function allele

(Edgar and Datar, 1996)

Mutagen

ethyl methanesulfonate

(Mozer and Easwarachandran, 1999, Tearle and Nusslein-Volhard, 1987)

Progenitor genotype

Cytology

Description

Point mutant.

(Mozer and Easwarachandran, 1999)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

abdominal neuroblast NB6-4

(Akiyama-Oda et al., 2000)

cardioblast

(Han and Bodmer, 2003)

central nervous system | embryonic stage

(Edgar and O'Farrell, 1990)

centriole

(Vidwans et al., 1999)

chordotonal organ

(Vervoort et al., 1997)

ectoderm | embryonic stage

(Hartenstein and Posakony, 1990)

embryo

(Han and Bodmer, 2003, Edgar and O'Farrell, 1990)

embryonic/larval crystal cell | ectopic (with Df(2L)200)

(Bataille et al., 2005)

embryonic neuroblast

(Tio et al., 2001)

external sensory organ

(Vervoort et al., 1997)

follicle cell | somatic clone

(Deng et al., 2001)

imaginal disc

(Lehman et al., 1999)

macrochaeta

(Mozer and Easwarachandran, 1999)

macrochaeta (with stg^hwy)

(Mozer and Easwarachandran, 1999)

mitotic domain 1 | embryonic cycle 14

(Vidwans et al., 1999)

multidendritic neuron

(Vervoort et al., 1997)

pericardial cell

(Han and Bodmer, 2003)

thoracic neuroblast NB6-4

(Akiyama-Oda et al., 2000)

wing disc

(Neufeld et al., 1998)

Detailed Description

Statement

Reference

In a stg² mutant context, Df(2L)200 mutant embryos induce a twofold increase in the number of crystal cells.

(Bataille et al., 2005)

In some segments in mutant embryos tin or eve expressing cells fail to form. Mutants also show severe segmentation and other patterning defects.

(Han and Bodmer, 2003)

Homozygous follicle cell clones have far fewer cells than their wild-type sister clones. The mean ratio of the number of cells in the mutant clone/number of cells in the wild-type sister clone is 0.1 (compared to a ratio of 1.0 in control experiments).

(Deng et al., 2001)

In embryos lacking zygotic stg⁺, neuroblasts form but arrest at G2 during interphase of cell-cycle stage 14 and fail to enter mitosis.

(Tio et al., 2001)

In homozygous mutant stg² embryos, single large mononucleated cells with NB6-4 identity are seen in most of the thoracic segments and 90% of abdominal hemisegments. This is in contrast to wild-type where NB6-4T divides into 5 to 6 cells, and NB6-4A divides into 2 cells.

(Akiyama-Oda et al., 2000)

Postblastoderm cell divisions are completely blocked in stg² mutants. Imaginal disc cells that are homozygous for stg² divide only once, producing 2-celled clones that are eliminated by cell competition.

(Lehman et al., 1999)

Eye of adults heterozygous with stg^hwy are normal, though missing macrochaetae phenotype remains.

(Mozer and Easwarachandran, 1999)

The number of eve-expressing mesodermal cells per hemisegment is similar in stg² single mutant and stg²; zfh1² double mutant embryos.

(Su et al., 1999)

Mutant embryos arrest in G2 of cycle 14, while development continues well after the time of mitosis 14. Two centriole pairs are seen per cell. More than 95% of the pairs have incompletely assembled daughters (the daughter centrioles are significantly shorter than their mother centrioles and their tubular fine structure is not obvious).

(Vidwans et al., 1999)

Cells in homozygous clones induced in the wing pouch of the wing disc divide only once and become enlarged. These cells are gradually lost from the disc epithelium.

(Neufeld et al., 1998)

All cell divisions are blocked after the blastoderm stage. In embryos sense organ precursors do not divide but do differentiate into neurons, several md but only a few es neurons are observed.

(Vervoort et al., 1997)

Embryos derived from germ line clones proceed through cycles 1-13 normally and always transverse the maternal/zygotic transition (MZT) during interphase.

(Edgar and Datar, 1996)

Cell division arrested.

(Li and Kaufman, 1996)

Lethal interaction with noose¹, noose², noose³ and noose⁴. Transheterozygotes with stg^knot are viable and fertile.

(White-Cooper et al., 1996)

Expression of dap^GMR.PdN transgene in stg² flies has no effect on the eye phenotype.

(de Nooij et al., 1996)

Neuroblast formation occurs normally in stg mutant embryos.

(Cui and Doe, 1995)

Malpighian tubule phenotype examined in embryos and found to be similar to that of wg mutants: tubules remain as clusters of approximately 20 cells. However in stg² all four primordia appear. Tip cells are present and tubules go on to elongate and produce uric acid.

(Skaer and Martinez Arias, 1992)

No mitoses after mitosis 13 but individuals still develop to secrete a cuticle that is virtually devoid of differentiated pattern elements. Failure of head involution, few thoracic or abdominal denticles develop and tail structures are rudimentary. Introduction of the stg^hs.PE2 construct without heat shock causes differentiation of more cuticular structure due to leaky expression. Heat shock 2 hours after egg deposition synchronous cell divisions that bore no resemblance to the normal mitotic pattern. Greater number of heat pulses increased the extent of normal cuticle differentiation, 3 pulses gave normal cuticle. These embryos do not hatch as the there are too few CNS cells.

(Edgar and O'Farrell, 1990)

No cell division occurs in the ectoderm after the blastoderm stage in homozygotes.

(Hartenstein and Posakony, 1990)

stg² embryos first deviate from normal development during the early stages of gastrulation, when they fail to initiate the 14th mitosis. There is no evidence of chromosome condensation, nuclear envelope breakdown or spindle formation between 3 and 12 hours after egg deposition (at 25^oC), in contrast to wild type embryos. The cells are arrested in G2. Mutants show a nonspecific reduction in the number of differentiated cuticular structures.

(Edgar and O'Farrell, 1989)

strong allele

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

stg² is an enhancer of visible phenotype of Scer\GAL4^en-e16E, arm^UAS.cWa

(Greaves et al., 1999)

NOT Enhancer of

Statement

Reference

stg² is a non-enhancer of visible phenotype of Dp^GMR.PD, E2f1^GMR.PD

(Staehling-Hampton et al., 1999)

Suppressor of

Statement

Reference

stg²/stg[+] is a suppressor of increased cell death phenotype of shtd¹

(Tanaka-Matakatsu et al., 2007)

stg² is a suppressor of visible phenotype of Scer\GAL4^en-e16E, shg^{i.UAS.Tag:SS(aos),Tag:MYC}

(Greaves et al., 1999)

NOT Suppressor of

Statement

Reference

stg² is a non-suppressor of visible phenotype of Dp^GMR.PD, E2f1^GMR.PD

(Staehling-Hampton et al., 1999)

Other

Statement

Reference

stg², twe¹ has decreased cell number | oogenesis phenotype

(Edgar and Datar, 1996)

stg², twe¹ has decreased occurrence of cell division | oogenesis phenotype

(Edgar and Datar, 1996)

Phenotype Manifest In

Enhancer of

Statement

Reference

stg² is an enhancer of wing margin bristle | ectopic phenotype of Scer\GAL4^en-e16E, arm^UAS.cWa

(Marygold and Vincent, 2003)

stg² is an enhancer of wing phenotype of Scer\GAL4^en-e16E, arm^UAS.cWa

(Greaves et al., 1999)

NOT Enhancer of

Statement

Reference

stg² is a non-enhancer of phenotype of Myb^unspecified

(Fung et al., 2003)

Suppressor of

Statement

Reference

stg²/stg[+] is a suppressor of eye phenotype of shtd¹

(Tanaka-Matakatsu et al., 2007)

stg²/stg[+] is a suppressor of ommatidium phenotype of shtd¹

(Tanaka-Matakatsu et al., 2007)

stg² is a suppressor of wing phenotype of Scer\GAL4^en-e16E, shg^{i.UAS.Tag:SS(aos),Tag:MYC}

(Greaves et al., 1999)

NOT Suppressor of

Statement

Reference

stg² is a non-suppressor of phenotype of Myb^unspecified

(Fung et al., 2003)

stg²/stg[+] is a non-suppressor of lamina anlage & mitotic cell cycle phenotype of dally⁰⁶⁴⁶⁴

(Nakato et al., 2002)

Other

Statement

Reference

stg², twe¹ has ovariole phenotype

(Edgar and Datar, 1996)

stg², twe¹ has female germline cell | oogenesis phenotype

(Edgar and Datar, 1996)

Additional Comments

Genetic Interactions

Statement

Reference

The addition of stg² has no effect on Myb^unspecified viability.

(Fung et al., 2003)

One copy of stg² does not rescue the second division in dally⁰⁶⁴⁶⁴ lamina precursor neurons and appears to expand the first G2/M domain of lamina precursor neuron division.

(Nakato et al., 2002)

Expression of cdc2^AF.hs in a stg² mutant background drives the G2-arrested stg mutant cells into mitosis.

(Su et al., 2001)

In stg²/stg⁴,Df(3L)ri-79c double mutant embryos no post-blastoderm cell divisions occur and gastrulation is normal.

(Seher and Leptin, 2000)

Simultaneous deletion of stg²/stg² twe¹/twe¹ causes a block in germ cell proliferation, no ovarioles or eggs are produced.

(Edgar and Datar, 1996)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially complements

stg^hwy

(Mozer and Easwarachandran, 1999)

Fails to complement

stg^Dr-L2

(Mozer, 2001)

Rescued by

stg² is rescued by stg^t31.6

(Deng et al., 2001)

stg² is rescued by Scer\GAL4^eg-Mz360/stg^UAS.cNa

(Akiyama-Oda et al., 2000)

stg² is rescued by Scer\GAL4^en-e16E/stg^UAS.cNa

(Akiyama-Oda et al., 2000)

Partially rescued by

stg² is partially rescued by stg^BCD

(Deng et al., 2001)

stg² is partially rescued by stg^BCD

(Lehman et al., 1999)

stg² is partially rescued by stg^t31.6

(Lehman et al., 1999)

stg² is partially rescued by stg^CD

(Lehman et al., 1999)

stg² is partially rescued by stg^t6.0

(Lehman et al., 1999)

stg² is partially rescued by stg^hs.PE2

(Edgar and O'Farrell, 1990)

Comments

stg^BCD partially rescues the clone size of stg² follicle cell clones; mutant clones half the size of wild-type sister clones are detected. The nuclei of the follicle cells are twice the size of the nuclei of wild-type sister clone cells.

(Deng et al., 2001)

The cell division phenotype is partially rescued by stg^t31.6; cell cycle progression is seen in a subset of the wild-type division pattern, and the embryos die with mild cuticular defects which can be attributed to a partial loss of cell division in mitotic domain 11 (the dorsolateral epidermis). The imaginal disc phenotype is rescued by stg^t31.6, stg^BCD, stg^CD or stg^t6.0; stg² cells in the imaginal disc carrying one of these transgenes divide many times and produce large clones of cells. stg² clones rescued by stg^t31.6 are equal in size to their wild-type sister clones, while stg² clones rescued by any of the other transgenes are smaller than their wild-type sister clones and show increased cell size. stg² cells rescued by stg^t6.0 have an increased G₂ phase.

(Lehman et al., 1999)

Complements stg^hwy for ommatidial phenotype, fails to complement stg^hwy for bristle phenotype.

(Mozer and Easwarachandran, 1999)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Strong stg allele.

(Li and Kaufman, 1996)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

(Shermoen and O'Farrell, 1991)

stg²

stg⁷

(Han and Bodmer, 2003, Akiyama-Oda et al., 2000)

stg^7B69

(Akiyama-Oda et al., 2000, Seher and Leptin, 2000, Crozatier et al., 1999, Su et al., 1999, Carmena et al., 1998, Lu et al., 1998, Vervoort et al., 1997, Crozatier et al., 1996, Li and Kaufman, 1996, Duronio and O'Farrell, 1994, Reuter et al., 1993, Skaer and Martinez Arias, 1992, Gould et al., 1990, Hartenstein and Posakony, 1990, Edgar and O'Farrell, 1989)

string7B

(Vidwans et al., 1999)

string^7b69

(Fung et al., 2003)

Name Synonyms

Secondary FlyBase IDs

FBal0016174
FBal0099824
FBal0244503

References (61)

Report Sections

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