FB2024_03 , released June 25, 2024
Allele: Dmel\stghs.PE2
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General Information
Symbol
Dmel\stghs.PE2
Species
D. melanogaster
Name
heat shock construct of Edgar
FlyBase ID
FBal0035720
Feature type
allele
Associated gene
Dmel\stg
Associated Insertion(s)
Carried in Construct
P{hs-stg.RK}
Also Known As
Hs-stg
Key Links
Transgenic product class
wild_type
(Edgar and O'Farrell, 1990)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Edgar and O'Farrell, 1990)
Mutagen
in vitro construct
(Edgar and O'Farrell, 1990)
Progenitor genotype
Carried in construct
P{hs-stg.RK}
Cytology
Description

stg gene expression is under the control of Hsp70 promoter.

(Edgar and O'Farrell, 1990)
Allele components
Component
Use(s)
Regulatory region(s)
Hsp70
Encoded product / tool
stg
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      There is a delay in GMC-1 entering G2-phase in embryos that are ectopically expressing stg.

      (Wai et al., 1999)

      stg overproduction induces mitoses in the dorsal and ventral anterior domains of the ZNC in the developing wing.

      (Johnston and Edgar, 1998)

      Heat induced expression causes precocious mitoses and the formation of sensory mother cells is disturbed, resulting in the loss of macrochaetes in the adult notum.

      (Kimura et al., 1997)

      Heat shock of wandering stage third instar larvae causes cells, across all developmental compartments of the wing disk, to enter premature mitoses. Ectopically induced mitoses display the normal progression of mitotic events. Ectopic induction results in mitoses in 30% of wing disc cells. Complete mitoses occur among the bristle progenitor cells as a consequence of ectopic stg expression. The minimum heat shock time required to induce mitoses is approximately 10 minutes. Abdominal histoblasts are refractory to ectopic stg expression. Ectopic stg expression results in mitoses just posterior to the furrow in the eye disc, no mitoses are observed in the furrow itself. Induced mitoses in eye discs show normal progression of mitotic events. Heat induced third instar larvae develop into adults indistinguishable from wild-type.

      (Kylsten and Saint, 1997)

      Heat shock 160-185 minutes after egg deposition causes the mitotic times of all embryonic cells, except those in the amnioserosa and mitotic domain B that are normally nondividing, to advance. The length of the S phase is unaltered. Heat shocked stg proteins can only induce mitotic divisions in G2 cells. Heat shock of G2 cells of cycle 14 causes all cells, including those from the amnioserosa, to enter S phase. By varying the time of heat pulses cells can only be induced to divide very early in G2 of interphase 16.

      (Edgar and O'Farrell, 1990)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressor of
      NOT Suppressor of
      Statement
      Reference

      CycBΔ90, CycB3Δ, stghs.PE2 is a non-suppressor of abnormal mitotic cell cycle | embryonic stage phenotype of CycAC8LR1

      (Reber et al., 2006)
      Phenotype Manifest In
      Suppressor of
      Statement
      Reference

      stghs.PE2/CycEUAS.cRa, Scer\GAL4en.PU is a suppressor of wing disc phenotype of Ras85DV12.UAS, Scer\GAL4en.PU, dlg1GD4689

      (Willecke et al., 2011)

      stghs.PE2 is a suppressor of wing hair phenotype of Myb2

      (Katzen et al., 1998)
      NOT Suppressor of
      Statement
      Reference

      CycBΔ90, CycB3Δ, stghs.PE2 is a non-suppressor of embryonic epidermis phenotype of CycAC8LR1

      (Reber et al., 2006)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Wing disc compartments expressing Ras85DV12.Scer\UAS, dlg1GD4689, Pi3K92EGD11228, together with CycEScer\UAS.cRa and stghs.PE2 show a rescue in compartment size and high levels of His3-positive cells.

      (Willecke et al., 2011)

      Coexpression of the transgenes, cdc2hs.PS, CycBΔ90, and CycB3Δ, does not restore the entry of epidermal cells in CycAC8LR1 embryos into mitosis 16.

      (Reber et al., 2006)

      Myb2 mutant pupae carrying stghs.PE2 raised at 25oC exhibit partial suppression of the wing phenotype, the number of hairs is increased by 30-35% and the orientation of the hairs is corrected.

      (Katzen et al., 1998)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      stghs.PE2 rescues stghwy

      (Mozer and Easwarachandran, 1999)
      Partially rescues

      stghs.PE2 partially rescues stg2

      (Edgar and O'Farrell, 1990)
      Comments

      Rescues the retinal extra photoreceptor phenotype of stghwy mutants.

      (Mozer and Easwarachandran, 1999)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      stghs.PE2
      Name Synonyms
      heat shock construct of Edgar
      (Edgar and O'Farrell, 1990)
      Secondary FlyBase IDs
        References (12)