FlyBase Allele Report: Dmel\CycA[UAS.cHa]

General Information

Symbol

Dmel\CycA^UAS.cHa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Hayashi

FlyBase ID

FBal0097377

Feature type

allele

Associated gene

Dmel\CycA

Associated Insertion(s)

Carried in Construct

P{UAS-CycA.H}

Key Links

Transgenic product class

cDNA

(Hayashi and Yamaguchi, 1999)

wild_type

(Hayashi and Yamaguchi, 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Hayashi and Yamaguchi, 1999)

wild_type

(Hayashi and Yamaguchi, 1999)

Mutagen

in vitro construct

(Hayashi and Yamaguchi, 1999)

Progenitor genotype

Carried in construct

P{UAS-CycA.H}

Cytology

Description

A CycA cDNA is expressed under the control of UASt regulatory sequences.

(Hayashi and Yamaguchi, 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

CycA

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of CycA^Scer\UAS.cHa in the salivary glands under the control of Scer\GAL4^l(1)BP-1 has very little effect on endoreplication at 25^oC.

(Hayashi and Yamaguchi, 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

Cdk1^UAS.cHa, CycA^UAS.cHa, Scer\GAL4^l(1)BP-1 has embryonic/larval salivary gland | embryonic stage phenotype, suppressible by E2f1^hs.PA

(Hayashi and Yamaguchi, 1999)

Suppressor of

Statement

Reference

Scer\GAL4^P127, CycA^UAS.cHa, Xlae\cdc2^K33R.UAS is a suppressor of histoblast & nucleus phenotype of Cdk1^E1-24/Cdk1^B47

(Hayashi and Yamaguchi, 1999)

Scer\GAL4^P127/CycA^UAS.cHa is a suppressor of histoblast & nucleus phenotype of Cdk1^E1-24/Cdk1^B47

(Hayashi and Yamaguchi, 1999)

NOT Suppressor of

Statement

Reference

Scer\GAL4^P127, CycA^UAS.cHa, Xlae\cdc2^K33R.UAS is a non-suppressor of imaginal disc phenotype of Cdk1^E1-24/Cdk1^B47

(Hayashi and Yamaguchi, 1999)

Other

Statement

Reference

CycA^UAS.cHa, Scer\GAL4^l(1)BP-1, Xlae\cdc2^K33R.UAS has embryonic/larval salivary gland phenotype

(Hayashi and Yamaguchi, 1999)

CycA^UAS.cHa, Scer\GAL4^l(1)BP-1, Xlae\cdc2^UAS.cHa has embryonic/larval salivary gland phenotype

(Hayashi and Yamaguchi, 1999)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of both CycA^Scer\UAS.cHa and cdc2^Scer\UAS.cHa in the salivary glands under the control of Scer\GAL4^l(1)BP-1 strongly prevents endoreplication and growth in the salivary glands. The defects in the abdominal histoblasts seen in cdc2^B47/cdc2^E1-24 larvae at the restrictive temperature are partially rescued by CycA^Scer\UAS.cHa expressed under the control of Scer\GAL4^P127; the increase in DNA content is limited to 8C. The inhibition of endoreplication in the salivary gland caused by coexpression of both CycA^Scer\UAS.cHa and cdc2^Scer\UAS.cHa under the control of Scer\GAL4^l(1)BP-1 is reversed if the larvae are also expressing E2f^hs.PA.

(Hayashi and Yamaguchi, 1999)

Xenogenetic Interactions

Statement

Reference

Coexpression of CycA^Scer\UAS.cHa and Xlae\cdc2^{K33R.Scer\UAS} under the control of Scer\GAL4^P127 completely rescues the abdominal histoblast defects seen in cdc2^B47/cdc2^E1-24 larvae at the restrictive temperature. The imaginal disc defects seen in these larvae are not rescued. Expression of both CycA^Scer\UAS.cHa and Xlae\cdc2^Scer\UAS.cHa in the salivary glands under the control of Scer\GAL4^l(1)BP-1 strongly inhibits endoreplication. Expression of both CycA^Scer\UAS.cHa and Xlae\cdc2^{K33R.Scer\UAS} in the salivary glands under the control of Scer\GAL4^l(1)BP-1 strongly inhibits endoreplication.

(Hayashi and Yamaguchi, 1999)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

83154

w^*; P{UAS-CycA.H}3

Notes on Origin

Discoverer

Comments

Carried in a plasmid, transfected into Kc cells and used in transcription activation assays.

(Hayashi and Yamaguchi, 1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

CycA^Scer\UAS.cHa

CycA^UAS.cHa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Hayashi

(Hayashi and Yamaguchi, 1999)

Secondary FlyBase IDs

References (2)

Report Sections

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