FB2024_03 , released June 25, 2024
Allele: Xlae\cdc2K33R.UAS
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General Information
Symbol
Xlae\cdc2K33R.UAS
Species
X. laevis
Name
FlyBase ID
FBal0097087
Feature type
allele
Associated gene
Xlae\cdc2
Associated Insertion(s)
Carried in Construct
P{UAS-Xlae\cdc2.K33R}
Transgenic product class
missense_variant
(Hayashi and Yamaguchi, 1999)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Hayashi and Yamaguchi, 1999)
Mutagen
in vitro construct
(Hayashi and Yamaguchi, 1999)
Progenitor genotype
Carried in construct
P{UAS-Xlae\cdc2.K33R}
Cytology
Description

A kinase defective form of Xlae\cdc2 (with the amino acid replacement: K33R) is expressed under the control of UAS regulatory sequences.

(Hayashi and Yamaguchi, 1999)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Xlae\cdc2
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressor of
      Statement
      Reference

      Scer\GAL4P127, CycAUAS.cHa, Xlae\cdc2K33R.UAS is a suppressor of histoblast & nucleus phenotype of Cdk1E1-24/Cdk1B47

      (Hayashi and Yamaguchi, 1999)
      NOT Suppressor of
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      Does not suppress the mitotic defects seen in cdc2B47/cdc2E1-24 larvae at the restrictive temperature when expressed under the control of Scer\GAL4P127. Coexpression of CycAScer\UAS.cHa and Xlae\cdc2K33R.Scer\UAS under the control of Scer\GAL4P127 completely rescues the abdominal histoblast defects seen in cdc2B47/cdc2E1-24 larvae at the restrictive temperature. The imaginal disc defects seen in these larvae are not rescued. Expression of both CycAScer\UAS.cHa and Xlae\cdc2K33R.Scer\UAS in the salivary glands under the control of Scer\GAL4l(1)BP-1 strongly inhibits endoreplication.

      (Hayashi and Yamaguchi, 1999)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Xlae\cdc2K33R.Scer\UAS
      Xlae\cdc2K33R.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (1)