FB2024_03 , released June 25, 2024
Allele: Dmel\Cdk1WT.UASp.Venus
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General Information
Symbol
Dmel\Cdk1WT.UASp.Venus
Species
D. melanogaster
Name
FlyBase ID
FBal0294232
Feature type
allele
Associated gene
Dmel\Cdk1
Associated Insertion(s)
Carried in Construct
P{UASp-Cdk1.WT.VFP}
Also Known As
Cdk1WT, Cdk1(WT)-VFP
Key Links
Transgenic product class
wild_type
(Ayeni et al., 2014)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Ayeni et al., 2014)
Mutagen
in vitro construct
(Ayeni et al., 2014)
Progenitor genotype
Carried in construct
P{UASp-Cdk1.WT.VFP}
Cytology
Description

UASp sequences regulate expression of wild type Cdk1 tagged at the C terminus with Venus.

(Ayeni et al., 2014)
Allele components
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
Cdk1
Tagged with
Venus
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Adult males expressing Cdk1WT.Scer\UAS.P\T.T:Avic\GFP-YFP.Venus under the control of Scer\GAL4bam.T:Hsim\VP16 are fertile and their spermatocytes expressing have normal bipolar meiotic spindles with engaged centrioles.

      (Varadarajan et al., 2016)

      Adult-specific expression of Cdk1WT.Scer\UAS.P\T.T:Avic\GFP-YFP.Venus (under the control of RU486-dependent Scer\GAL4elav.Switch.PO) has little effect on sleep.

      (Afonso et al., 2015)

      Wing development is not notably affected by expression of cdc2WT.Scer\UAS.P\T.T:Avic\GFP-YFP.Venus via Scer\GAL4sd.PU.

      Eye development is unaffected by expression of cdc2WT.Scer\UAS.P\T.T:Avic\GFP-YFP.Venus via Scer\GAL4ey.PU.

      Scer\GAL4en.PU-mediated expression of cdc2WT.Scer\UAS.P\T.T:Avic\GFP-YFP.Venus does not alter apoptosis or the mitotic index in the posterior of the wing imaginal disc.

      One hour after exposure to 40-Gy ionizing radiation, Scer\GAL4en.PU cdc2WT.Scer\UAS.P\T.T:Avic\GFP-YFP.Venus wing discs do not show mitotic cells, indicating a normal checkpoint response.

      (Ayeni et al., 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Wing development is not notably affected by co-expression of cdc2WT.Scer\UAS.P\T.T:Avic\GFP-YFP.Venus and CycBScer\UAS.T:Ivir\HA1 via Scer\GAL4sd.PU.

      (Ayeni et al., 2014)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Cdk1WT.UASp.Venus/Scer\GAL4Tub.PU rescues Cdk1E1-24/Cdk1B47

      (Ayeni et al., 2014)
      Comments

      Scer\GAL4tub.PU-mediated expression of cdc2WT.Scer\UAS.P\T.T:Avic\GFP-YFP.Venus rescues the pupal lethality of cdc2B47/cdc2E1-24 animals grown at the restrictive temperature of 25[o]C.

      (Ayeni et al., 2014)
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Cdk1WT.Scer\UAS.P\T.T:Avic\GFP-YFP.Venus
      Cdk1WT.UASp.Venus
      cdc2WT.Scer\UAS.P\T.T:Avic\GFP-YFP.Venus
      Name Synonyms
      Secondary FlyBase IDs
        References (4)