FB2024_03 , released April 23, 2024
Allele: Scer\GAL4NP7175
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General Information
Symbol
Scer\GAL4NP7175
Species
S. cerevisiae
Name
FlyBase ID
FBal0159793
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
P{GawB}hfwNP7175
Carried in Construct
Also Known As
NP7175-GAL4, NP7175
Genomic Maps

Allele class
Mutagen
    Nature of the Allele
    Expression Data
    Reporter Expression
    distribution deduced from reporter (Gal4 UAS)
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    third instar larval stage
    inner layer of the larval mushroom body
    (Pauls et al., 2010, Pauls et al., 2010)
    outer layer of the larval mushroom body
    (Pauls et al., 2010, Pauls et al., 2010)
    core layer of the larval mushroom body
    (Pauls et al., 2010, Pauls et al., 2010)
    adult stage
    adult antennal lobe projection neuron | subset
    (Tanaka et al., 2004)
    adult mushroom body alpha-lobe | restricted

    Comment: core of mushroom body alpha-lobe

    (Tanaka et al., 2004)
    adult mushroom body beta-lobe | restricted

    Comment: core of mushroom body beta-lobe

    (Tanaka et al., 2004)
    calyx of adult mushroom body
    (Tanaka et al., 2008)
    mushroom body beta lobe core layer | subset
    (Tanaka et al., 2008)
    mushroom body alpha lobe core layer | subset
    (Tanaka et al., 2008)
    adult mushroom body | restricted
    (Tanaka et al., 2004)
    alpha/beta Kenyon cell
    (Pauls et al., 2010)
    alpha/beta core Kenyon cell
    (Flybrain Neuron Database et al., 2018.6.28, Perisse et al., 2013)
    Kenyon cell
    (Flybrain Neuron Database et al., 2018.6.28)
    adult mushroom body alpha-lobe
    (Pauls et al., 2010)
    adult mushroom body beta-lobe
    (Pauls et al., 2010)
    adult stage | female
    mushroom body beta lobe core layer
    (Aso et al., 2009)
    mushroom body alpha lobe core layer
    (Aso et al., 2009)
    medulla
    (Aso et al., 2009)
    adult tritocerebrum
    (Aso et al., 2009)
    adult gnathal ganglion | faint
    (Aso et al., 2009)
    glial cell of adult brain | faint
    (Aso et al., 2009)
    adult stage | male
    mushroom body beta lobe core layer | intense
    (Aso et al., 2009)
    mushroom body alpha lobe core layer | intense
    (Aso et al., 2009)
    medulla | intense
    (Aso et al., 2009)
    adult tritocerebrum
    (Aso et al., 2009)
    adult gnathal ganglion | faint
    (Aso et al., 2009)
    glial cell of adult brain
    (Aso et al., 2009)
    Additional Information
    Statement
    Reference

    Expression of ScerGAL4NP7175 is found within the inner regions of the alpha and beta core stratum and not the outer core.

    (Tanaka et al., 2008)

    Expression is observed in 46-70 projection neurons in the cores of the α and β lobes of the mushroom body.

    (Tanaka et al., 2004)
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Disease-implicated variant(s)
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    Detailed Description
    Statement
    Reference

    Blocking synaptic transmission (using shi1.Scer\UAS at restrictive temperatures) from Scer\GAL4NP7175 neurons significantly impairs sucrose-reinforced appetitive memory performance (at 3 or 24 hours since training) compared to controls. Blocking synaptic transmission from Scer\GAL4NP7175 neurons does not significantly impair electric-shock-reinforced aversive memory performance (at 3hrs) compared to controls. Blocking synaptic transmission from Scer\GAL4NP7175 neurons significantly impairs memory (30 minutes after conditioning) in a differential aversive conditioning paradigm (in the Y[[60]] vs Z[[30]] relative condition but not the X[[0]] vs Y[[60]] absolute condition). Flies undergo conditioning at a permissive temperature (23[o]C), whereas testing is at a restrictive temperature (33[o]C); there are no significant memory differences when training and testing is at permissive temperatures.

    (Perisse et al., 2013)

    Blocking synaptic transmission either during acquisition (during spaced training) or during retrieval (at the time of testing, 24 hours after training) in neurons expressing Scer\GAL4NP7175 has no effect on aversive long term memory. Long term memory is impaired when synaptic transmission is blocked over the whole consolidation period or in the 12 hour interval 6 hours after completion of spaced training.

    Sensorimotor abilities are impaired when synaptic transmission is blocked in Scer\GAL4NP7175-expressing neurons when compared to controls without the driver, but is not significantly different from the driver only controls.

    Blocking synaptic transmission in Scer\GAL4NP7175-expressing neurons for the 12 hour interval 6 hours after massed training does not impair anesthesia-resistant (ARM) memory.

    Blocking synaptic transmission in Scer\GAL4NP7175-expressing neurons during the post-training period has no effect on appetitive long term memory (association of an odor with sucrose).

    (Huang et al., 2012)
    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    Suppressor of
    NOT Suppressor of
    Phenotype Manifest In
    Additional Comments
    Genetic Interactions
    Statement
    Reference
    Xenogenetic Interactions
    Statement
    Reference
    Complementation and Rescue Data
    Images (0)
    Mutant
    Wild-type
    Stocks (1)
    Notes on Origin
    Discoverer
    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (1)
    Reported As
    Symbol Synonym
    Scer\GAL4NP7175
    Name Synonyms
    Secondary FlyBase IDs
      References (20)