FlyBase Allele Report: Dmel\sick[Δ]

General Information

Symbol

Dmel\sick^Δ

Species

D. melanogaster

Name

FlyBase ID

FBal0301618

Feature type

allele

Associated gene

Dmel\sick

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

FLPase

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Abe et al., 2014)

Mutagen

FLPase

(Abe et al., 2014)

Progenitor genotype

P{XP}sick^d09251

(Abe et al., 2014)

P{XP}sick^d07725

(Abe et al., 2014)

Caused by aberration

Df(2L)sick^Δ

Cytology

Description

The 520 amino acids corresponding to the third proline-rich region and the first CC motif of sick have been deleted. The non-coding genes CR43828, CR43827 and CR45183 are also deleted.

(Abe et al., 2014)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2L:19,918,015..19,935,139 [+]

Comment:

Scer\FRT-mediated recombination between the two progenitor insertions has resulted in the deletion of the genomic sequence between them.

(Abe et al., 2014)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy

(Abe et al., 2014)

abnormal neuroanatomy (with Df(2L)ED1303)

(Abe et al., 2014)

abnormal neuroanatomy (with sick^A384)

(Abe et al., 2014)

abnormal neuroanatomy (with sick^A384), with Scer\GAL4^elav.PU, sick^UAS.cAa

(Abe et al., 2014)

abnormal neuroanatomy (with sick^Δ-2)

(Abe et al., 2014)

abnormal neuroanatomy (with sick^Δ-L1)

(Abe et al., 2014)

abnormal neuroanatomy | somatic clone

(Abe et al., 2014)

Phenotype Manifest In

adult mushroom body alpha-lobe

(Abe et al., 2014)

adult mushroom body alpha-lobe (with Df(2L)ED1303)

(Abe et al., 2014)

adult mushroom body alpha-lobe (with sick^A384)

(Abe et al., 2014)

adult mushroom body alpha-lobe (with sick^Δ-2)

(Abe et al., 2014)

adult mushroom body alpha-lobe (with sick^Δ-L1)

(Abe et al., 2014)

adult mushroom body alpha-lobe (with sick^Δ-L2)

(Abe et al., 2014)

adult mushroom body alpha-lobe (with sick^Δ-L3)

(Abe et al., 2014)

adult mushroom body alpha-lobe | somatic clone

(Abe et al., 2014)

adult mushroom body beta-lobe

(Abe et al., 2014)

adult mushroom body beta-lobe (with Df(2L)ED1303)

(Abe et al., 2014)

adult mushroom body beta-lobe (with sick^A384)

(Abe et al., 2014)

adult mushroom body beta-lobe (with sick^A384), with Scer\GAL4^elav.PU, sick^UAS.cAa

(Abe et al., 2014)

adult mushroom body beta-lobe (with sick^Δ-2)

(Abe et al., 2014)

adult mushroom body beta-lobe (with sick^Δ-L1)

(Abe et al., 2014)

adult mushroom body beta-lobe (with sick^Δ-L2)

(Abe et al., 2014)

adult mushroom body beta-lobe (with sick^Δ-L3)

(Abe et al., 2014)

adult mushroom body beta-lobe | somatic clone

(Abe et al., 2014)

Detailed Description

Statement

Reference

Homozygous sick^Δ mutant mushroom bodies display short α/β axonal lobes compared with those of controls. No axonal lobe phenotypes are observed in the mushroom bodies of sick^Δ/+ mutants.

The axons of single sick^Δ mutant α/β mushroom body neurons fail to extend their branched axons to the lobe terminus.

sick^Δ mutant clones in the core region of the α/β lobe of the mushroom body show slightly increased F-actin levels compared with wild type controls.

sick^A384/sick^Δ mutant mushroom bodies display short α/β axonal lobes compared with those of controls, although the morphology of the peduncle appears normal. In addition, sick^A384/sick^Δ mutants display defective ellipsoid body ring structures in each hemisphere of the central brain. A prominent increase in F-actin signal is detected in the core region of the defective α/β axonal lobe compared to controls.

sick^Δ/Df(2L)ED1303 mutant mushroom bodies display short α/β axonal lobes compared with those of controls.

sick^Δ/sick^Δ-L1 mutant mushroom bodies display short α/β axonal lobes compared with those of controls.

sick^Δ/sick^Δ-L2 mutant mushroom bodies display short α/β axonal lobes compared with those of controls.

sick^Δ/sick^Δ-L3 mutant mushroom bodies display short α/β axonal lobes compared with those of controls.

sick^Δ/sick^Δ-2 mutant mushroom bodies display short α/β axonal lobes compared with those of controls.

(Abe et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

sick^A384/sick^Δ has abnormal neuroanatomy phenotype, suppressible | partially by Scer\GAL4^NP7175/tsr^UAS.cNa

(Abe et al., 2014)

sick^A384/sick^Δ has abnormal neuroanatomy phenotype, suppressible | partially by Scer\GAL4^NP7175/tsr^S3A.UAS

(Abe et al., 2014)

sick^A384/sick^Δ has abnormal neuroanatomy phenotype, suppressible | partially by ssh^UAS.Tag:HA/Scer\GAL4^NP7175

(Abe et al., 2014)

NOT suppressed by

Statement

Reference

sick^A384/sick^Δ has abnormal neuroanatomy phenotype, non-suppressible by Scer\GAL4^NP7175/tsr^S3E.UAS

(Abe et al., 2014)

sick^A384/sick^Δ has abnormal neuroanatomy phenotype, non-suppressible by Scer\GAL4^NP7175/ssh^{CS.UAS.Tag:HA}

(Abe et al., 2014)

NOT Suppressor of

Statement

Reference

Rac1^{Y40C.UAS.Tag:MYC}, sick^A384, sick^Δ, Scer\GAL4^ey-OK107 is a non-suppressor of abnormal neuroanatomy phenotype of LIMK1^UAS.Tag:HA, Scer\GAL4^ey-OK107

(Abe et al., 2014)

ssh^UAS.Tag:HA, sick^A384, sick^Δ, Scer\GAL4^ey-OK107 is a non-suppressor of abnormal neuroanatomy phenotype of LIMK1^UAS.Tag:HA, Scer\GAL4^ey-OK107

(Abe et al., 2014)

Other

Statement

Reference

Mtl[+]/Mtl^Δ, Rac1^J11, Rac2^Δ/Rac2[+], sick^Δ, tsr[+]/tsr^N96A has abnormal neuroanatomy phenotype

(Abe et al., 2014)

Phenotype Manifest In

Suppressed by

Statement

Reference

sick^A384/sick^Δ has adult mushroom body alpha-lobe phenotype, suppressible | partially by Scer\GAL4^NP7175/tsr^UAS.cNa

(Abe et al., 2014)

sick^A384/sick^Δ has adult mushroom body beta-lobe phenotype, suppressible | partially by Scer\GAL4^NP7175/tsr^UAS.cNa

(Abe et al., 2014)

sick^A384/sick^Δ has adult mushroom body alpha-lobe phenotype, suppressible | partially by Scer\GAL4^NP7175/tsr^S3A.UAS

(Abe et al., 2014)

sick^A384/sick^Δ has adult mushroom body beta-lobe phenotype, suppressible | partially by Scer\GAL4^NP7175/tsr^S3A.UAS

(Abe et al., 2014)

sick^A384/sick^Δ has adult mushroom body alpha-lobe phenotype, suppressible | partially by ssh^UAS.Tag:HA/Scer\GAL4^NP7175

(Abe et al., 2014)

sick^A384/sick^Δ has adult mushroom body beta-lobe phenotype, suppressible | partially by ssh^UAS.Tag:HA/Scer\GAL4^NP7175

(Abe et al., 2014)

NOT suppressed by

Statement

Reference

sick^A384/sick^Δ has adult mushroom body alpha-lobe phenotype, non-suppressible by Scer\GAL4^NP7175/tsr^S3E.UAS

(Abe et al., 2014)

sick^A384/sick^Δ has adult mushroom body beta-lobe phenotype, non-suppressible by Scer\GAL4^NP7175/tsr^S3E.UAS

(Abe et al., 2014)

sick^A384/sick^Δ has adult mushroom body alpha-lobe phenotype, non-suppressible by Scer\GAL4^NP7175/ssh^{CS.UAS.Tag:HA}

(Abe et al., 2014)

sick^A384/sick^Δ has adult mushroom body beta-lobe phenotype, non-suppressible by Scer\GAL4^NP7175/ssh^{CS.UAS.Tag:HA}

(Abe et al., 2014)

NOT Suppressor of

Statement

Reference

Rac1^{Y40C.UAS.Tag:MYC}, sick^A384, sick^Δ, Scer\GAL4^ey-OK107 is a non-suppressor of adult mushroom body beta-lobe phenotype of LIMK1^UAS.Tag:HA, Scer\GAL4^ey-OK107

(Abe et al., 2014)

ssh^UAS.Tag:HA, sick^A384, sick^Δ, Scer\GAL4^ey-OK107 is a non-suppressor of adult mushroom body alpha-lobe phenotype of LIMK1^UAS.Tag:HA, Scer\GAL4^ey-OK107

(Abe et al., 2014)

ssh^UAS.Tag:HA, sick^A384, sick^Δ, Scer\GAL4^ey-OK107 is a non-suppressor of adult mushroom body beta-lobe phenotype of LIMK1^UAS.Tag:HA, Scer\GAL4^ey-OK107

(Abe et al., 2014)

Rac1^{Y40C.UAS.Tag:MYC}, sick^A384, sick^Δ, Scer\GAL4^ey-OK107 is a non-suppressor of mushroom body phenotype of LIMK1^UAS.Tag:HA, Scer\GAL4^ey-OK107

(Abe et al., 2014)

Rac1^{Y40C.UAS.Tag:MYC}, sick^A384, sick^Δ, Scer\GAL4^ey-OK107 is a non-suppressor of adult mushroom body alpha-lobe phenotype of LIMK1^UAS.Tag:HA, Scer\GAL4^ey-OK107

(Abe et al., 2014)

Other

Statement

Reference

Mtl[+]/Mtl^Δ, Rac1^J11, Rac2^Δ/Rac2[+], sick^Δ, tsr[+]/tsr^N96A has mushroom body pedunculus phenotype

(Abe et al., 2014)

Mtl[+]/Mtl^Δ, Rac1^J11, Rac2^Δ/Rac2[+], sick^Δ, tsr[+]/tsr^N96A has adult mushroom body beta-lobe phenotype

(Abe et al., 2014)

Mtl[+]/Mtl^Δ, Rac1^J11, Rac2^Δ/Rac2[+], sick^Δ, tsr[+]/tsr^N96A has adult mushroom body alpha-lobe phenotype

(Abe et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

sick^Δ tsr^N96A double heterozygotes do not show obvious axonal growth defects in α/β axonal lobes, peduncle and ellipsoid body.

The mushroom body axons of flies that are heterozygous for Rac1^J11, Rac2^Δ tsr^N96A sick^Δ and Mtl^Δ fail to extend to form peduncles and lobe structures (the 'posterior arrest' phenotype).

Expression of tsr^Scer\UAS.cNa under the control of Scer\GAL4^NP7175 significantly suppresses the axon growth phenotype seen in the α/β mushroom body lobes of sick^A384/sick^Δ mutants.

Expression of tsr^S3A.Scer\UAS under the control of Scer\GAL4^NP7175 significantly suppresses the axon growth phenotype seen in the α/β mushroom body lobes of sick^A384/sick^Δ mutants.

Expression of tsr^S3E.Scer\UAS under the control of Scer\GAL4^NP7175 does not significantly suppresses the axon growth phenotype seen in the α/β mushroom body lobes of sick^A384/sick^Δ mutants.

Expression of ssh^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^NP7175 partially suppresses the axon growth phenotype seen in the α/β mushroom body lobes of sick^A384/sick^Δ mutants.

Expression of ssh^{CS.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^NP7175 does not significantly suppress the axon growth phenotype seen in the α/β mushroom body lobes of sick^A384/sick^Δ mutants.

Expression of ssh^{Scer\UAS.T:Ivir\HA1} suppresses the axon growth defect and F-actin elevation seen in the α/β mushroom body lobes when LIMK1^{Scer\UAS.T:Ivir\HA1} is expressed under the control of Scer\GAL4^ey-OK107. This suppression does not occur in a sick^Δ/sick^A384 mutant background.

Expression of Rac1^{Y40C.Scer\UAS.T:Hsap\MYC} suppresses the axon growth defect and F-actin elevation seen in the α/β mushroom body lobes when LIMK1^{Scer\UAS.T:Ivir\HA1} is expressed under the control of Scer\GAL4^ey-OK107. This suppression does not occur in a sick^Δ/sick^A384 mutant background.

(Abe et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

sick^A384/sick^Δ is partially rescued by Scer\GAL4^elav.PU/sick^UAS.cAa

(Abe et al., 2014)

sick^A384/sick^Δ is partially rescued by Scer\GAL4^ey-OK107/sick^UAS.cAa

(Abe et al., 2014)

sick^A384/sick^Δ is partially rescued by Scer\GAL4^NP7175/sick^UAS.cAa

(Abe et al., 2014)

sick^A384/sick^Δ is partially rescued by Scer\GAL4^elav.PU/sick^UAS.mCherry

(Abe et al., 2014)

sick^A384/sick^Δ is partially rescued by Scer\GAL4^ey-OK107/sick^UAS.mCherry

(Abe et al., 2014)

sick^A384/sick^Δ is partially rescued by Scer\GAL4^NP7175/sick^UAS.mCherry

(Abe et al., 2014)

Not rescued by

sick^A384/sick^Δ is not rescued by Scer\GAL4^ey-OK107/sick^{K1881A.UAS.mCherry}

(Abe et al., 2014)

sick^A384/sick^Δ is not rescued by Scer\GAL4^NP7175/sick^{K1881A.UAS.mCherry}

(Abe et al., 2014)

Comments

Expression of sick^Scer\UAS.cAa under the control of Scer\GAL4^elav.PU almost fully rescues the axonal growth defects seen in the α/β mushroom body lobes of sick^Δ/sick^A384 mutants. The rescued flies exhibit a β lobe fusion phenotype.

Expression of sick^Scer\UAS.cAa under the control of Scer\GAL4^ey-OK107 significantly rescues the axonal growth defects seen in the α/β mushroom body lobes of sick^Δ/sick^A384 mutants.

Expression of sick^Scer\UAS.cAa under the control of Scer\GAL4^NP7175 significantly rescues the axonal growth defects seen in the α/β mushroom body lobes of sick^Δ/sick^A384 mutants.

Expression of sick^{Scer\UAS.T:Disc\RFP-mCherry} under the control of Scer\GAL4^elav.PU almost fully rescues the axonal growth defects seen in the α/β mushroom body lobes of sick^Δ/sick^A384 mutants.

Expression of sick^{Scer\UAS.T:Disc\RFP-mCherry} under the control of Scer\GAL4^ey-OK107 significantly rescues the axonal growth defects seen in the α/β mushroom body lobes of sick^Δ/sick^A384 mutants.

Expression of sick^{Scer\UAS.T:Disc\RFP-mCherry} under the control of Scer\GAL4^NP7175 significantly rescues the axonal growth defects seen in the α/β mushroom body lobes of sick^Δ/sick^A384 mutants.

Expression of sick^{K1881A.Scer\UAS.T:Disc\RFP-mCherry} under the control of Scer\GAL4^ey-OK107 fails to rescue the axonal growth defects seen in the α/β mushroom body lobes of sick^Δ/sick^A384 mutants.

Expression of sick^{K1881A.Scer\UAS.T:Disc\RFP-mCherry} under the control of Scer\GAL4^NP7175 fails to rescue the axonal growth defects seen in the α/β mushroom body lobes of sick^Δ/sick^A384 mutants.

(Abe et al., 2014)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

sick^Δ

Name Synonyms

sickieΔ

(Abe et al., 2014)

Secondary FlyBase IDs

References (1)

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