FlyBase Allele Report: Dmel\tsr[UAS.cNa]

General Information

Symbol

Dmel\tsr^UAS.cNa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Ng

FlyBase ID

FBal0177368

Feature type

allele

Associated gene

Dmel\tsr

Associated Insertion(s)

Carried in Construct

P{UAS-tsr.N}

Also Known As

UAS-tsr WT

Key Links

Transgenic product class

wild_type

(Ng and Luo, 2004)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Ng and Luo, 2004)

Mutagen

in vitro construct

(Ng and Luo, 2004)

Progenitor genotype

Carried in construct

P{UAS-tsr.N}

Cytology

Description

UASt regulatory sequences drive expression of the entire tsr ORF.

(Ng and Luo, 2004)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

tsr

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^109(2)80

(Medina et al., 2008)

Phenotype Manifest In

dendrite, with Scer\GAL4^109(2)80

(Medina et al., 2008)

filamentous actin | larval stage | somatic clone, with Scer\GAL4^Act.PU

(Farrell et al., 2022)

wing disc | larval stage | somatic clone, with Scer\GAL4^Act.PU

(Farrell et al., 2022)

Detailed Description

Statement

Reference

Expression of tsr^Scer\UAS.cNa (using tub-Gal80[ts] to limit the expression to the adult stage) under the control of Scer\GAL4^elav.PU does not significantly alter 3 hr memory of adult flies in an aversive olfactory assay compared to controls.

(Shuai et al., 2010)

Expression of tsr^Scer\UAS.cNa under the control of Scer\GAL4^109(2)80 results in the formation of Act5C^{Scer\UAS.T:Avic\GFP} accumulations in neuronal dendrites, with occasional rod-like structures forming.

(Medina et al., 2008)

Overexpression of tsr^Scer\UAS.cNa in mushroom body neurons under the control of Scer\GAL4^OK107 does not result in gross axon or cell proliferation defects.

(Ng and Luo, 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^109(2)80, tsr^UAS.cNa has abnormal neuroanatomy phenotype, enhanceable by capt^K304/capt^K304

(Medina et al., 2008)

Suppressed by

Statement

Reference

Scer\GAL4^109(2)80, tsr^UAS.cNa has abnormal neuroanatomy phenotype, suppressible by Scer\GAL4^109(2)80/capt^UAS.cBa

(Medina et al., 2008)

Suppressor of

Statement

Reference

tsr^UAS.cNa, Scer\GAL4^ey.PU is a suppressor | partially of visible | adult stage phenotype of BacA\p35^UAS.cUa, Scer\GAL4^ey.PU, hid^UAS.cUa

(Farrell et al., 2022)

tsr^UAS.cNa, Scer\GAL4^ey.PU is a suppressor of increased occurrence of cell division | larval stage phenotype of BacA\p35^UAS.cUa, Scer\GAL4^ey.PU, hid^UAS.cUa

(Farrell et al., 2022)

Scer\GAL4^NP7175/tsr^UAS.cNa is a suppressor | partially of abnormal neuroanatomy phenotype of sick^A384/sick^Δ

(Abe et al., 2014)

Scer\GAL4^ey-OK107/tsr^UAS.cNa is a suppressor of abnormal neuroanatomy | somatic clone phenotype of ssh^1-11

(Ng and Luo, 2004)

tsr^UAS.cNa, Scer\GAL4^ey-OK107 is a suppressor of abnormal neuroanatomy phenotype of LIMK1^UAS.Tag:HA, Scer\GAL4^ey-OK107

(Ng and Luo, 2004)

tsr^UAS.cNa, Scer\GAL4^ey-OK107 is a suppressor | partially of abnormal neuroanatomy phenotype of Pak^UAS.Tag:MYC, Scer\GAL4^ey-OK107

(Ng and Luo, 2004)

NOT Suppressor of

Statement

Reference

Scer\GAL4^mirr-DE/tsr^UAS.cNa is a non-suppressor of decreased size | adult stage phenotype of hid^GMR.PG

(Farrell et al., 2022)

Scer\GAL4^mirr-DE/tsr^UAS.cNa is a non-suppressor of increased cell death | larval stage phenotype of hid^GMR.PG

(Farrell et al., 2022)

Other

Statement

Reference

Scer\GAL4^mirr-DE, hid^UAS.cUa, tsr^UAS.cNa has abnormal size | adult stage phenotype

(Farrell et al., 2022)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^109(2)80, tsr^UAS.cNa has dendrite phenotype, enhanceable by capt^K304/capt^K304

(Medina et al., 2008)

Suppressed by

Statement

Reference

Scer\GAL4^109(2)80, tsr^UAS.cNa has dendrite phenotype, suppressible by Scer\GAL4^109(2)80/capt^UAS.cBa

(Medina et al., 2008)

Suppressor of

Statement

Reference

tsr^UAS.cNa, Scer\GAL4^ey.PU is a suppressor | partially of adult head phenotype of BacA\p35^UAS.cUa, Scer\GAL4^ey.PU, hid^UAS.cUa

(Farrell et al., 2022)

tsr^UAS.cNa, Scer\GAL4^ey.PU is a suppressor of eye disc | larval stage phenotype of BacA\p35^UAS.cUa, Scer\GAL4^ey.PU, hid^UAS.cUa

(Farrell et al., 2022)

tsr^UAS.cNa, Scer\GAL4^ey.PU is a suppressor of filamentous actin | larval stage phenotype of BacA\p35^UAS.cUa, Scer\GAL4^ey.PU, hid^UAS.cUa

(Farrell et al., 2022)

tsr^UAS.cNa, Scer\GAL4^Act.PU is a suppressor of eye disc | somatic clone | larval stage phenotype of BacA\p35^UAS.cUa, Scer\GAL4^Act.PU, hid^UAS.cUa

(Farrell et al., 2022)

tsr^UAS.cNa, Scer\GAL4^Act.PU is a suppressor of filamentous actin | somatic clone | larval stage phenotype of BacA\p35^UAS.cUa, Scer\GAL4^Act.PU, hid^UAS.cUa

(Farrell et al., 2022)

Scer\GAL4^NP7175/tsr^UAS.cNa is a suppressor | partially of adult mushroom body alpha-lobe phenotype of sick^A384/sick^Δ

(Abe et al., 2014)

Scer\GAL4^NP7175/tsr^UAS.cNa is a suppressor | partially of adult mushroom body beta-lobe phenotype of sick^A384/sick^Δ

(Abe et al., 2014)

Scer\GAL4^ey-OK107/tsr^UAS.cNa is a suppressor of adult mushroom body & axon | somatic clone phenotype of ssh^1-11

(Ng and Luo, 2004)

Scer\GAL4^ey-OK107/tsr^UAS.cNa is a suppressor of antennal lobe & axon | somatic clone phenotype of ssh^1-11

(Ng and Luo, 2004)

Scer\GAL4^ey-OK107/tsr^UAS.cNa is a suppressor of optic lobe neuron & axon | somatic clone phenotype of ssh^1-11

(Ng and Luo, 2004)

tsr^UAS.cNa, Scer\GAL4^ey-OK107 is a suppressor of adult mushroom body phenotype of LIMK1^UAS.Tag:HA, Scer\GAL4^ey-OK107

(Ng and Luo, 2004)

tsr^UAS.cNa, Scer\GAL4^ey-OK107 is a suppressor | partially of adult mushroom body phenotype of Pak^UAS.Tag:MYC, Scer\GAL4^ey-OK107

(Ng and Luo, 2004)

NOT Suppressor of

Statement

Reference

Scer\GAL4^mirr-DE/tsr^UAS.cNa is a non-suppressor of eye disc | larval stage phenotype of hid^GMR.PG

(Farrell et al., 2022)

Scer\GAL4^mirr-DE/tsr^UAS.cNa is a non-suppressor of eye | adult stage phenotype of hid^GMR.PG

(Farrell et al., 2022)

Other

Statement

Reference

Scer\GAL4^mirr-DE, hid^UAS.cUa, tsr^UAS.cNa has eye disc | larval stage phenotype

(Farrell et al., 2022)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of tsr^Scer\UAS.cNa under the control of Scer\GAL4^NP7175 significantly suppresses the axon growth phenotype seen in the α/β mushroom body lobes of sick^A384/sick^Δ mutants.

(Abe et al., 2014)

Longer rod-like structures of Act5C^{Scer\UAS.T:Avic\GFP} accumulation are formed in the dendrites of animals expressing tsr^Scer\UAS.cNa under the control of Scer\GAL4^109(2)80 when the animals are also homozygous for capt^K304.

No rod-like structures of Act5C^{Scer\UAS.T:Avic\GFP} accumulation are formed in the dendrites of animals expressing tsr^Scer\UAS.cNa under the control of Scer\GAL4^109(2)80 is they are also co-expressing capt^Scer\UAS.cBa.

(Medina et al., 2008)

The axon growth and guidance defects seen in homozygous ssh^1-11 adult mushroom body clones (which are generated as neuroblast clones in newly hatched larvae) are almost completely suppressed by co-expression of tsr^Scer\UAS.cNa under the control of Scer\GAL4^OK107. The axon growth defects seen in neuroblast clones of optic lobe and antennal lobe contralateral projection neurons are partially suppressed by co-expression of tsr^Scer\UAS.cNa under the control of Scer\GAL4^OK107.

(Ng and Luo, 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^ey-OK107/tsr^UAS.cNa rescues tsr^N96A

(Ng and Luo, 2004)

Comments

Expression of tsr^Scer\UAS.cNa under the control of Scer\GAL4^OK107 fully rescues the cell proliferation defects seen in homozygous tsr^N96A adult mushroom body clones (which are generated as neuroblast clones in newly hatched larvae). Expression of tsr^Scer\UAS.cNa under the control of Scer\GAL4^OK107 effectively rescues the axon growth defects seen in single cell tsr^N96A γ neuron clones in the mushroom body.

(Ng and Luo, 2004)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

9234

P{UAS-tsr.N}1.7, y¹ w^*; βTub60D^Pin-1/CyO

9235

y¹ w^*; P{UAS-tsr.N}2.2.1/TM6B, P{Car20y}TPN1, Tb¹

Notes on Origin

Discoverer